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The map label for this gene is amiA [H]

Identifier: 89055027

GI number: 89055027

Start: 2537283

End: 2538488

Strand: Reverse

Name: amiA [H]

Synonym: Jann_2536

Alternate gene names: 89055027

Gene position: 2538488-2537283 (Counterclockwise)

Preceding gene: 89055028

Following gene: 89055025

Centisome position: 58.79

GC content: 66.09

Gene sequence:

>1206_bases
GTGTTTCGAGCCGTCCTGATCATCGCCCTCCTGTGGTTTGGCGCGCCGTCGATGGCGCAGACGCTTTCGGCTGCCGCCCG
GGTCTTGCCCGAAGGGTCGGTGCTGGAGGGCAATCGCAGCGCGACCGAATTGCGGCTGACCCTCAGCCAGGCGGTGCCGT
TCCGCGTCTTCACCCTGACCGACCCGATGCGCGTGGTGCTGGACTTCCGAACCGTCGATTTCAGCGCGCTGCCTGCGGCA
TTCGGCAACGCACCGGATGTGGTGGGGGTTCAGATGGGCGGGGCCTCCACCCCGGGCTGGTCCCGGATGATCCTGACGCT
CGGCGCGCCCTTATCGCTGGATGTGGCCGCGATGGAGACGGATACCATTACCGGAGAAGCCGTTGTTTCGCTGACCCTTT
CGCCCATTGATGCCGATATGTTTGCACAGGCCGCGGGTGCACCGCCGGGCCTTGATGCGATGCTCCTGCCCACGGTCACC
CAGGCCCTGCCGACGGAGGATGACACGTTTGTGGTGATGCTCGACCCCGGCCATGGCGGTATCGATCCCGGTGCCCTGCG
GGAGGGGTTCTCGGAGGCCGAGCTGATCCTGACCTTTGCCCGGGAGCTGCGCGAAGTCATGCGCCGCACTGGTCAAATCG
AGGTTCAGATGACCCGCGATGCCGATATTTTCGTGCCGCTGCCGACCCGCGTGACGCTGGCCCGGGCGGTGGGGGCCGAC
CTGTTCATCTCGCTCCACGCCGACGCGATTGCCGAGGGGCGCGCGCAGGGCGCCACGGTCTATACGCTGTCCGATGAGGC
CACGGATGCCGCCACCGCAGCGCTGGCGGAACAGCACGACCGCGCGGATCTGTTGCAGGGGATCGACCTGTCCGGCAGCG
ATGATGAGGTCGTTGGTGTGTTGCTGGATCTTGCCCGGATCGAGACCGCGCCGCGCAGCCGTGCCTTCGCCGATACCCTC
GTGCAAGCCATTGATATGGCGGGGTTGGATTTGCACGCGCGGCCCCGGGGCGAAGGGGCGTTTTCCGTGCTGAAGGCGGC
AGATTTTCCGGCCGTGTTGCTGGAAATCGGGTTTCTCAGCGAAGGCGGAGACCTGGAGAACATTCAGAACCCGGAGTGGC
GGGCGCAGATGCAGGCGGCCATTACCGATGCGGTGTTGTCTTGGTCGCAGGATGATGCCGCTGCGGCGGCGCTCAGGCGT
CAGTAA

Upstream 100 bases:

>100_bases
AGGGCGTCGCGCGCCTGATGACGTTCCTCAACGCCCTCTAGGCCCGCCCGTCCCGATCCGCTAACCTGCGCGCAACCAAT
CAGAGGGAAGCAGCCCCCCC

Downstream 100 bases:

>100_bases
ACCGCGCGAGCCAGACGCTGTGGCCGCGCAATTCCGCATCTTCGGGGCGAAACCAGACGAGTTCAAACCCATGGGTGGCC
AGAAGGTTGCGGTATTCATC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MFRAVLIIALLWFGAPSMAQTLSAAARVLPEGSVLEGNRSATELRLTLSQAVPFRVFTLTDPMRVVLDFRTVDFSALPAA
FGNAPDVVGVQMGGASTPGWSRMILTLGAPLSLDVAAMETDTITGEAVVSLTLSPIDADMFAQAAGAPPGLDAMLLPTVT
QALPTEDDTFVVMLDPGHGGIDPGALREGFSEAELILTFARELREVMRRTGQIEVQMTRDADIFVPLPTRVTLARAVGAD
LFISLHADAIAEGRAQGATVYTLSDEATDAATAALAEQHDRADLLQGIDLSGSDDEVVGVLLDLARIETAPRSRAFADTL
VQAIDMAGLDLHARPRGEGAFSVLKAADFPAVLLEIGFLSEGGDLENIQNPEWRAQMQAAITDAVLSWSQDDAAAAALRR
Q

Sequences:

>Translated_401_residues
MFRAVLIIALLWFGAPSMAQTLSAAARVLPEGSVLEGNRSATELRLTLSQAVPFRVFTLTDPMRVVLDFRTVDFSALPAA
FGNAPDVVGVQMGGASTPGWSRMILTLGAPLSLDVAAMETDTITGEAVVSLTLSPIDADMFAQAAGAPPGLDAMLLPTVT
QALPTEDDTFVVMLDPGHGGIDPGALREGFSEAELILTFARELREVMRRTGQIEVQMTRDADIFVPLPTRVTLARAVGAD
LFISLHADAIAEGRAQGATVYTLSDEATDAATAALAEQHDRADLLQGIDLSGSDDEVVGVLLDLARIETAPRSRAFADTL
VQAIDMAGLDLHARPRGEGAFSVLKAADFPAVLLEIGFLSEGGDLENIQNPEWRAQMQAAITDAVLSWSQDDAAAAALRR
Q
>Mature_401_residues
MFRAVLIIALLWFGAPSMAQTLSAAARVLPEGSVLEGNRSATELRLTLSQAVPFRVFTLTDPMRVVLDFRTVDFSALPAA
FGNAPDVVGVQMGGASTPGWSRMILTLGAPLSLDVAAMETDTITGEAVVSLTLSPIDADMFAQAAGAPPGLDAMLLPTVT
QALPTEDDTFVVMLDPGHGGIDPGALREGFSEAELILTFARELREVMRRTGQIEVQMTRDADIFVPLPTRVTLARAVGAD
LFISLHADAIAEGRAQGATVYTLSDEATDAATAALAEQHDRADLLQGIDLSGSDDEVVGVLLDLARIETAPRSRAFADTL
VQAIDMAGLDLHARPRGEGAFSVLKAADFPAVLLEIGFLSEGGDLENIQNPEWRAQMQAAITDAVLSWSQDDAAAAALRR
Q

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=35.4700854700855, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87082163, Length=373, Percent_Identity=28.1501340482574, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1790611, Length=227, Percent_Identity=29.0748898678414, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 42419; Mature: 42419

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRAVLIIALLWFGAPSMAQTLSAAARVLPEGSVLEGNRSATELRLTLSQAVPFRVFTLT
CHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEEECCCCEEEEEEC
DPMRVVLDFRTVDFSALPAAFGNAPDVVGVQMGGASTPGWSRMILTLGAPLSLDVAAMET
CCEEEEEEEEECCCHHHHHHCCCCCCEEEEEECCCCCCCHHEEEEEECCCCEEEEEEEEC
DTITGEAVVSLTLSPIDADMFAQAAGAPPGLDAMLLPTVTQALPTEDDTFVVMLDPGHGG
CCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCC
IDPGALREGFSEAELILTFARELREVMRRTGQIEVQMTRDADIFVPLPTRVTLARAVGAD
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHCCE
LFISLHADAIAEGRAQGATVYTLSDEATDAATAALAEQHDRADLLQGIDLSGSDDEVVGV
EEEEECHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHH
LLDLARIETAPRSRAFADTLVQAIDMAGLDLHARPRGEGAFSVLKAADFPAVLLEIGFLS
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHCCCCEEEHEEECCC
EGGDLENIQNPEWRAQMQAAITDAVLSWSQDDAAAAALRRQ
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MFRAVLIIALLWFGAPSMAQTLSAAARVLPEGSVLEGNRSATELRLTLSQAVPFRVFTLT
CHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEECCCCCCEEEEEEEECCCCEEEEEEC
DPMRVVLDFRTVDFSALPAAFGNAPDVVGVQMGGASTPGWSRMILTLGAPLSLDVAAMET
CCEEEEEEEEECCCHHHHHHCCCCCCEEEEEECCCCCCCHHEEEEEECCCCEEEEEEEEC
DTITGEAVVSLTLSPIDADMFAQAAGAPPGLDAMLLPTVTQALPTEDDTFVVMLDPGHGG
CCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCC
IDPGALREGFSEAELILTFARELREVMRRTGQIEVQMTRDADIFVPLPTRVTLARAVGAD
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHCCE
LFISLHADAIAEGRAQGATVYTLSDEATDAATAALAEQHDRADLLQGIDLSGSDDEVVGV
EEEEECHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHH
LLDLARIETAPRSRAFADTLVQAIDMAGLDLHARPRGEGAFSVLKAADFPAVLLEIGFLS
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHCCCCEEEHEEECCC
EGGDLENIQNPEWRAQMQAAITDAVLSWSQDDAAAAALRRQ
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]