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The map label for this gene is ligA

Identifier: 89054995

GI number: 89054995

Start: 2503013

End: 2505271

Strand: Reverse

Name: ligA

Synonym: Jann_2504

Alternate gene names: 89054995

Gene position: 2505271-2503013 (Counterclockwise)

Preceding gene: 89054996

Following gene: 89054994

Centisome position: 58.02

GC content: 64.14

Gene sequence:

>2259_bases
ATGTCACAACCAGATCAAGAGGCGCAGGCGCTGGCCGCCGGTTTTTCGGCAGACGCGGCCAAAACACGTCTGGCGGAACT
GGCAAAGCGCCTCTCGGACGCCAACACTGCCTACCACGCCGCAGACGCCCCGGATCTGAGCGATGCCGATTACGACGCCC
TGAAGCGAGAGAATGCCGCGATCGAGGCCGCATTTCCGGACCTCAAGCGCGCCGACAGTCCCAGTGATCAGGTCGGTGCG
GCCCCTGCGGAGGGGTTCTCGAAACTCACTCACACCCAACGCATGCTGTCGCTCGCCAACGCGTTTGACGCGGGCGATGT
CCCTGATTTTGTGGCAGGTATTCGTCGCTACCTGAACCTTGGCGCGGAGGCGGATCTTCCCTTCACCGCCGAGCCCAAGA
TTGACGGGCTGTCGCTGTCACTGCGATACGAGGACGGCCAGCTGGTCTCTGCGGCCACAAGGGGCGACGGGGCCATCGGC
GAAAACGTCACGGCAAATGCGCGCACCATCGATGATATTCCGCAAACCCTGACGGATGCGCCAGACGTTCTTGAGGTGCG
GGGCGAGGTCTACATGAGCCACGCGGACTTCGCCGCTCTGAACGACCGCCAGGCAGAGGCCGGGGCCAAGACCTTCGCCA
ATCCGCGCAACGCCGCCGCCGGATCGCTGCGGCAGTTGGATGCCTCCATCACCCGATCCCGCCCGCTGAGGTTCTTCGCC
TACGCCTGGGGAGAGGTGAGTGCGCCCCTTGCGGCCACCCAATCGGGCGCGCTCAACACGCTGAAAACACTTGGATTCCA
GATCAACGACCGCACCAAGACGTGCCATAGCGCCGAGGACATGCTGGCCCATTATGCGAGCATTGAGCAGGACCGTGCCG
ATCTGGGCTATGACATCGACGGCGTGGTCTACAAGATCGACGATCTTGACTATCAGCGCAGACTTGGCATGCGGTCCACG
ACGCCGCGATGGGCGATTGCCCACAAATTCCCGGCTGAACTGGCCTGGACCACGTTGGAAGCCATCGACATTCAGGTTGG
CCGCACCGGTGCGCTCAGCCCCGTGGCGCGCCTGACGCCTGTGACGGTCGGCGGCGTTGTTGTGTCCAACGCCACGCTGC
ACAATGAAGACTATATTGCCGGCCGCGATGCGAACGGAGGTCCCATCCGGGACGGCAAGGACATCCGCGTCGGCGACCGT
GTTCAGGTCTACCGCGCGGGCGACGTGATCCCGAAGGTCGCTGATGTAGACCTCGCAAAACGCCCCGAAAACACAGCGAA
ATATGCCTTTCCGGACCATTGCCCCCAGTGCCAGTCCGAGGCGATCCGGGAGGAGGGCGATGCCGTGCGTCGCTGCACCG
GCGGCCTGATCTGCCCGGCGCAGGCTGTGGAAAAGCTGAAGCATTTCGTCAGTCGCGCGGCGTTTGACATAGATGGGCTC
GGCGCCAAACAGGTGGAGGCCTTCTACCTTGATGGCTGGATTGCCGAACCCGCAGACATCTTCCGCCTGAAGGAGAATTA
TGGCGAGGGCATGAAACAACTCAGGAACCGCGAGGGGTGGGGCGAGAAATCGGCGACCAGCCTGTTCAACGCAATCGACG
ACAAACGCAGCATCGCGTTGCATCGTCTGATCTTCGCGCTCGGTATCCGCCATGTGGGGGAGGTTGCGGCCTCGGACCTT
GCGCGCCATTTCACGACGTGGAAGGCCTTGGCAGAGATCGTGGACCAAGCCGCGCCCGCCGCCGAGGCGCATCGCCGCGC
GGAAGACGCCGTCCTGGCCGAGCGGAAGGCCGCCGCCGATGAGGGCCGCCGCGCCTCCCTGCAACCCCAACGCGACAAGG
CCTGGGCCGACACGCCGGAGCCTGCAAAGGCAGCCTGGGACGACCTGACCGGCATCGACGGCATCGGTGCGACGGTCGCG
GTGTCACTGGTCACGACCTTCCAGCAGGCCCGCGAGCGCGCCTCCATCGACCGTCTGATCACGGAACTCACCGATATCCA
GCCGCCTGAGGCCCGCGCGACCCAAAGTGCGGTTGCGGGCCTCACCGTGGTTTTTACGGGCGCACTGGAGCGCATGACGC
GGGCGGAGGCCAAGGCCCGGGCAGAGGCGATGGGCGCCAAGGTCTCAGGCTCCGTTTCCGCCAAGACAGATATCGTTGTG
GCGGGACCGGGGGCGGGGTCAAAAGAGAAGAAAGCCAAGGATTTAGGGATTGAACTTCTGACCGAAGAACAATGGCTGGA
CCGCATCGGCGATGCCTGA

Upstream 100 bases:

>100_bases
AAACAATCGGAGTTGTGCGCGGCACGAAGGCTCAACTCTGGACCAATCCGCAATCGCCTCCTATCACCTTCCCCATACGG
TTGCAGCTTCGGGGGCGCGC

Downstream 100 bases:

>100_bases
TCACATTCCAAAAGGCCGCCCTGAGATCCTGTTTCCCCTCTTTGCGGGGCTGGAAACCTTGGACGGGATCGGCCCCAAGA
CCGCGAAAAATTACGCGGGC

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 752; Mature: 751

Protein sequence:

>752_residues
MSQPDQEAQALAAGFSADAAKTRLAELAKRLSDANTAYHAADAPDLSDADYDALKRENAAIEAAFPDLKRADSPSDQVGA
APAEGFSKLTHTQRMLSLANAFDAGDVPDFVAGIRRYLNLGAEADLPFTAEPKIDGLSLSLRYEDGQLVSAATRGDGAIG
ENVTANARTIDDIPQTLTDAPDVLEVRGEVYMSHADFAALNDRQAEAGAKTFANPRNAAAGSLRQLDASITRSRPLRFFA
YAWGEVSAPLAATQSGALNTLKTLGFQINDRTKTCHSAEDMLAHYASIEQDRADLGYDIDGVVYKIDDLDYQRRLGMRST
TPRWAIAHKFPAELAWTTLEAIDIQVGRTGALSPVARLTPVTVGGVVVSNATLHNEDYIAGRDANGGPIRDGKDIRVGDR
VQVYRAGDVIPKVADVDLAKRPENTAKYAFPDHCPQCQSEAIREEGDAVRRCTGGLICPAQAVEKLKHFVSRAAFDIDGL
GAKQVEAFYLDGWIAEPADIFRLKENYGEGMKQLRNREGWGEKSATSLFNAIDDKRSIALHRLIFALGIRHVGEVAASDL
ARHFTTWKALAEIVDQAAPAAEAHRRAEDAVLAERKAAADEGRRASLQPQRDKAWADTPEPAKAAWDDLTGIDGIGATVA
VSLVTTFQQARERASIDRLITELTDIQPPEARATQSAVAGLTVVFTGALERMTRAEAKARAEAMGAKVSGSVSAKTDIVV
AGPGAGSKEKKAKDLGIELLTEEQWLDRIGDA

Sequences:

>Translated_752_residues
MSQPDQEAQALAAGFSADAAKTRLAELAKRLSDANTAYHAADAPDLSDADYDALKRENAAIEAAFPDLKRADSPSDQVGA
APAEGFSKLTHTQRMLSLANAFDAGDVPDFVAGIRRYLNLGAEADLPFTAEPKIDGLSLSLRYEDGQLVSAATRGDGAIG
ENVTANARTIDDIPQTLTDAPDVLEVRGEVYMSHADFAALNDRQAEAGAKTFANPRNAAAGSLRQLDASITRSRPLRFFA
YAWGEVSAPLAATQSGALNTLKTLGFQINDRTKTCHSAEDMLAHYASIEQDRADLGYDIDGVVYKIDDLDYQRRLGMRST
TPRWAIAHKFPAELAWTTLEAIDIQVGRTGALSPVARLTPVTVGGVVVSNATLHNEDYIAGRDANGGPIRDGKDIRVGDR
VQVYRAGDVIPKVADVDLAKRPENTAKYAFPDHCPQCQSEAIREEGDAVRRCTGGLICPAQAVEKLKHFVSRAAFDIDGL
GAKQVEAFYLDGWIAEPADIFRLKENYGEGMKQLRNREGWGEKSATSLFNAIDDKRSIALHRLIFALGIRHVGEVAASDL
ARHFTTWKALAEIVDQAAPAAEAHRRAEDAVLAERKAAADEGRRASLQPQRDKAWADTPEPAKAAWDDLTGIDGIGATVA
VSLVTTFQQARERASIDRLITELTDIQPPEARATQSAVAGLTVVFTGALERMTRAEAKARAEAMGAKVSGSVSAKTDIVV
AGPGAGSKEKKAKDLGIELLTEEQWLDRIGDA
>Mature_751_residues
SQPDQEAQALAAGFSADAAKTRLAELAKRLSDANTAYHAADAPDLSDADYDALKRENAAIEAAFPDLKRADSPSDQVGAA
PAEGFSKLTHTQRMLSLANAFDAGDVPDFVAGIRRYLNLGAEADLPFTAEPKIDGLSLSLRYEDGQLVSAATRGDGAIGE
NVTANARTIDDIPQTLTDAPDVLEVRGEVYMSHADFAALNDRQAEAGAKTFANPRNAAAGSLRQLDASITRSRPLRFFAY
AWGEVSAPLAATQSGALNTLKTLGFQINDRTKTCHSAEDMLAHYASIEQDRADLGYDIDGVVYKIDDLDYQRRLGMRSTT
PRWAIAHKFPAELAWTTLEAIDIQVGRTGALSPVARLTPVTVGGVVVSNATLHNEDYIAGRDANGGPIRDGKDIRVGDRV
QVYRAGDVIPKVADVDLAKRPENTAKYAFPDHCPQCQSEAIREEGDAVRRCTGGLICPAQAVEKLKHFVSRAAFDIDGLG
AKQVEAFYLDGWIAEPADIFRLKENYGEGMKQLRNREGWGEKSATSLFNAIDDKRSIALHRLIFALGIRHVGEVAASDLA
RHFTTWKALAEIVDQAAPAAEAHRRAEDAVLAERKAAADEGRRASLQPQRDKAWADTPEPAKAAWDDLTGIDGIGATVAV
SLVTTFQQARERASIDRLITELTDIQPPEARATQSAVAGLTVVFTGALERMTRAEAKARAEAMGAKVSGSVSAKTDIVVA
GPGAGSKEKKAKDLGIELLTEEQWLDRIGDA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=740, Percent_Identity=42.4324324324324, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI87082305, Length=548, Percent_Identity=22.992700729927, Blast_Score=89, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_JANSC (Q28PE1)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510446.1
- ProteinModelPortal:   Q28PE1
- STRING:   Q28PE1
- GeneID:   3934963
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2504
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q28PE1
- ProtClustDB:   CLSK933607
- BioCyc:   JSP290400:JANN_2504-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 80821; Mature: 80690

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 133-133 BINDING 131-131 BINDING 154-154 BINDING 189-189 BINDING 305-305 BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPDQEAQALAAGFSADAAKTRLAELAKRLSDANTAYHAADAPDLSDADYDALKRENAA
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHCCCE
IEAAFPDLKRADSPSDQVGAAPAEGFSKLTHTQRMLSLANAFDAGDVPDFVAGIRRYLNL
EECCCCHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
GAEADLPFTAEPKIDGLSLSLRYEDGQLVSAATRGDGAIGENVTANARTIDDIPQTLTDA
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCC
PDVLEVRGEVYMSHADFAALNDRQAEAGAKTFANPRNAAAGSLRQLDASITRSRPLRFFA
CHHHHHCCHHEEECCCHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEE
YAWGEVSAPLAATQSGALNTLKTLGFQINDRTKTCHSAEDMLAHYASIEQDRADLGYDID
EECCCCCCCHHHCCCCHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVVYKIDDLDYQRRLGMRSTTPRWAIAHKFPAELAWTTLEAIDIQVGRTGALSPVARLTP
CEEEEECCCCHHHHCCCCCCCCCEEEHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHHCC
VTVGGVVVSNATLHNEDYIAGRDANGGPIRDGKDIRVGDRVQVYRAGDVIPKVADVDLAK
EEECCEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHCCCHHC
RPENTAKYAFPDHCPQCQSEAIREEGDAVRRCTGGLICPAQAVEKLKHFVSRAAFDIDGL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GAKQVEAFYLDGWIAEPADIFRLKENYGEGMKQLRNREGWGEKSATSLFNAIDDKRSIAL
CHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HRLIFALGIRHVGEVAASDLARHFTTWKALAEIVDQAAPAAEAHRRAEDAVLAERKAAAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
EGRRASLQPQRDKAWADTPEPAKAAWDDLTGIDGIGATVAVSLVTTFQQARERASIDRLI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TELTDIQPPEARATQSAVAGLTVVFTGALERMTRAEAKARAEAMGAKVSGSVSAKTDIVV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
AGPGAGSKEKKAKDLGIELLTEEQWLDRIGDA
ECCCCCCHHHHHHHCCHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
SQPDQEAQALAAGFSADAAKTRLAELAKRLSDANTAYHAADAPDLSDADYDALKRENAA
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHCCCE
IEAAFPDLKRADSPSDQVGAAPAEGFSKLTHTQRMLSLANAFDAGDVPDFVAGIRRYLNL
EECCCCHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
GAEADLPFTAEPKIDGLSLSLRYEDGQLVSAATRGDGAIGENVTANARTIDDIPQTLTDA
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCC
PDVLEVRGEVYMSHADFAALNDRQAEAGAKTFANPRNAAAGSLRQLDASITRSRPLRFFA
CHHHHHCCHHEEECCCHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEE
YAWGEVSAPLAATQSGALNTLKTLGFQINDRTKTCHSAEDMLAHYASIEQDRADLGYDID
EECCCCCCCHHHCCCCHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVVYKIDDLDYQRRLGMRSTTPRWAIAHKFPAELAWTTLEAIDIQVGRTGALSPVARLTP
CEEEEECCCCHHHHCCCCCCCCCEEEHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHHCC
VTVGGVVVSNATLHNEDYIAGRDANGGPIRDGKDIRVGDRVQVYRAGDVIPKVADVDLAK
EEECCEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHCCCHHC
RPENTAKYAFPDHCPQCQSEAIREEGDAVRRCTGGLICPAQAVEKLKHFVSRAAFDIDGL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GAKQVEAFYLDGWIAEPADIFRLKENYGEGMKQLRNREGWGEKSATSLFNAIDDKRSIAL
CHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HRLIFALGIRHVGEVAASDLARHFTTWKALAEIVDQAAPAAEAHRRAEDAVLAERKAAAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
EGRRASLQPQRDKAWADTPEPAKAAWDDLTGIDGIGATVAVSLVTTFQQARERASIDRLI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TELTDIQPPEARATQSAVAGLTVVFTGALERMTRAEAKARAEAMGAKVSGSVSAKTDIVV
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
AGPGAGSKEKKAKDLGIELLTEEQWLDRIGDA
ECCCCCCHHHHHHHCCHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA