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The map label for this gene is gluQ [H]

Identifier: 89054991

GI number: 89054991

Start: 2498565

End: 2499449

Strand: Direct

Name: gluQ [H]

Synonym: Jann_2500

Alternate gene names: 89054991

Gene position: 2498565-2499449 (Clockwise)

Preceding gene: 89054990

Following gene: 89054993

Centisome position: 57.86

GC content: 62.94

Gene sequence:

>885_bases
ATGATCACCCGCTTCGCGCCGTCGCCTACGGGGCCCTTGCACCTGGGTCATGCCTATTCGGCGATCCTGGCCCACGACAT
GGCATTGGCTGAGGGGGGCCACTTTCTTCTGCGGATCGAAGACATTGACCGCCAGCGCTCCAAACCGGATTGGGAAGCGC
AGATCTACGATGATTTGCAATGGCTTGGTTTGCATTGGGACATGCCGGTCCTGCGTCAATCGCAAAGGATGGAGGCCTAC
CGCGCTGCCCTGTCTGATCTTTGGCAGCGCGGGTTTCTGTTTCCCTGCGACTGCTCCCGCCGCGATATCCAGGCGGCCCT
GTCGGCGCCTCAGGAAACGGCCAGCCCCCCACTTGGACCCGACGGGATCATCTATCCCGGCACCTGTCGCCACAGAACGC
CTCATCCCAATCATGCGGATCCCGGCCCGATGCCCGATGGGGTCGCCCTGCGCCTGCGCGTCGACGAACATCTGGGGGTA
TTTGCCACGCCTCGTGTCGGGGGCGACGTGTTCAGTTTTCGCGAAACCGGCCCGGGCCATTCCTCCGATGTCGCCTTCAC
CGTAGATGAGGCGATCGCGTCAATCGGCGATATCGCCCTGGCGCGGCCTGATTTCGGGACATCCTACCACCTGTCGGTCG
TGTTGGATGACGCGGCGCAGGGCGTCACGCATGTCACGCGGGGAGCCGATCTGTTTGAGGCCACGAAAATCCATGTGATG
TTGCAGAAGATCCTGGGCCTGCCCACGCCCCTCTACCATCACCACCGCCTGATCCGCGATGACACAGGCAAGCGCCTGGC
CAAACGCAATGACGCCCGCGCGATTGCCAAATACCGTGCGGATGGTGCCTCGCCCGACGACGTTCGTGCCATGGTCGGGC
TTTGA

Upstream 100 bases:

>100_bases
ATGTTCTGGCTGACAATATTGCCCGTGTGGAATACCGCGCCCACGGGCCACATCTGTCTGAAAAAGTCACAGGCTCCGAC
GTGATCGTACTTCTGCGGCA

Downstream 100 bases:

>100_bases
GCTATCGCCATCTTGGCTGGGGAGGCCGCAGAACGGGGGCAGCGCTCCCTATTCCGGCGCCATCAACTCCACCTCTTCAC
CGCTTGTGACCGAGGTATAG

Product: glutamyl-tRNA synthetase, class Ic

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MITRFAPSPTGPLHLGHAYSAILAHDMALAEGGHFLLRIEDIDRQRSKPDWEAQIYDDLQWLGLHWDMPVLRQSQRMEAY
RAALSDLWQRGFLFPCDCSRRDIQAALSAPQETASPPLGPDGIIYPGTCRHRTPHPNHADPGPMPDGVALRLRVDEHLGV
FATPRVGGDVFSFRETGPGHSSDVAFTVDEAIASIGDIALARPDFGTSYHLSVVLDDAAQGVTHVTRGADLFEATKIHVM
LQKILGLPTPLYHHHRLIRDDTGKRLAKRNDARAIAKYRADGASPDDVRAMVGL

Sequences:

>Translated_294_residues
MITRFAPSPTGPLHLGHAYSAILAHDMALAEGGHFLLRIEDIDRQRSKPDWEAQIYDDLQWLGLHWDMPVLRQSQRMEAY
RAALSDLWQRGFLFPCDCSRRDIQAALSAPQETASPPLGPDGIIYPGTCRHRTPHPNHADPGPMPDGVALRLRVDEHLGV
FATPRVGGDVFSFRETGPGHSSDVAFTVDEAIASIGDIALARPDFGTSYHLSVVLDDAAQGVTHVTRGADLFEATKIHVM
LQKILGLPTPLYHHHRLIRDDTGKRLAKRNDARAIAKYRADGASPDDVRAMVGL
>Mature_294_residues
MITRFAPSPTGPLHLGHAYSAILAHDMALAEGGHFLLRIEDIDRQRSKPDWEAQIYDDLQWLGLHWDMPVLRQSQRMEAY
RAALSDLWQRGFLFPCDCSRRDIQAALSAPQETASPPLGPDGIIYPGTCRHRTPHPNHADPGPMPDGVALRLRVDEHLGV
FATPRVGGDVFSFRETGPGHSSDVAFTVDEAIASIGDIALARPDFGTSYHLSVVLDDAAQGVTHVTRGADLFEATKIHVM
LQKILGLPTPLYHHHRLIRDDTGKRLAKRNDARAIAKYRADGASPDDVRAMVGL

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=300, Percent_Identity=29.3333333333333, Blast_Score=114, Evalue=9e-26,
Organism=Escherichia coli, GI1788743, Length=284, Percent_Identity=34.8591549295775, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI226510925, Length=276, Percent_Identity=36.231884057971, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71993394, Length=303, Percent_Identity=29.3729372937294, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324540, Length=294, Percent_Identity=27.5510204081633, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24665321, Length=296, Percent_Identity=30.0675675675676, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 32406; Mature: 32406

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRFAPSPTGPLHLGHAYSAILAHDMALAEGGHFLLRIEDIDRQRSKPDWEAQIYDDLQ
CCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCCCCHHHHHHCHH
WLGLHWDMPVLRQSQRMEAYRAALSDLWQRGFLFPCDCSRRDIQAALSAPQETASPPLGP
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHCCCCCCC
DGIIYPGTCRHRTPHPNHADPGPMPDGVALRLRVDEHLGVFATPRVGGDVFSFRETGPGH
CCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCEEECCCCCCCC
SSDVAFTVDEAIASIGDIALARPDFGTSYHLSVVLDDAAQGVTHVTRGADLFEATKIHVM
CCCEEEEHHHHHHHHHHHEEECCCCCCCEEEEEEECCHHCCHHHHHCCCHHHHHHHHHHH
LQKILGLPTPLYHHHRLIRDDTGKRLAKRNDARAIAKYRADGASPDDVRAMVGL
HHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MITRFAPSPTGPLHLGHAYSAILAHDMALAEGGHFLLRIEDIDRQRSKPDWEAQIYDDLQ
CCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCEEEEEEECCHHHCCCCCCHHHHHHCHH
WLGLHWDMPVLRQSQRMEAYRAALSDLWQRGFLFPCDCSRRDIQAALSAPQETASPPLGP
EEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHCCCCCCC
DGIIYPGTCRHRTPHPNHADPGPMPDGVALRLRVDEHLGVFATPRVGGDVFSFRETGPGH
CCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCEEECCCCCCCC
SSDVAFTVDEAIASIGDIALARPDFGTSYHLSVVLDDAAQGVTHVTRGADLFEATKIHVM
CCCEEEEHHHHHHHHHHHEEECCCCCCCEEEEEEECCHHCCHHHHHCCCHHHHHHHHHHH
LQKILGLPTPLYHHHRLIRDDTGKRLAKRNDARAIAKYRADGASPDDVRAMVGL
HHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA