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The map label for this gene is rutF [H]

Identifier: 89054832

GI number: 89054832

Start: 2348034

End: 2348531

Strand: Direct

Name: rutF [H]

Synonym: Jann_2341

Alternate gene names: 89054832

Gene position: 2348034-2348531 (Clockwise)

Preceding gene: 89054823

Following gene: 89054835

Centisome position: 54.38

GC content: 64.46

Gene sequence:

>498_bases
ATGGACGATCTGAAGGCGACGTTTCTGGAAGGCATGAGCCGGGCCGCGGCGTCGGTTTGCGTGGTGACGACCGATGGCCC
CGCTGGCCGGGCTGGCGTCACCGTCTCGGCCATGACGTCGATCTCTGCCGATGGGGAGGCGCCGACAATGCTGACCTGCC
TGAACGCGTCGTCGTCCTGCCTGCCGATCCTGCTGGAGAATGCGTGCTTTTGCATCAATGTCCTCAGCACCGGGCAGACC
GAGATTTCTGATGTCTTTTCCTCCCGCATTCCGGCCCCGGGCGGGGACAAATTCAACGCCGTGGCCCACACCGTTCTGGC
CAGCGGCGCGCCCTATCTCAGCGATGCATTGGTGGGCTTTGATTGCACGCTGGTCTCGGCTGAAAAGCTGGGGACGCACC
ATATCTGCATCGGCGCGGTTCAGGCCGTGCGGGTTGCGCCCGAGGGGGAACCGCTGCTGTACGGCATGCGCAAGTACCTG
CGGGCGGAGACCCACTGA

Upstream 100 bases:

>100_bases
AGAAATCGCGAGACGTAGCCCCGAATTGATCCATCTTGTGCGGGGATCGGTGGTGGGCGATGGTCATGCGCGTCTTTGGC
GTGTCAGTTTGGGGAATGTG

Downstream 100 bases:

>100_bases
AGGTCCTGTTCAGCCGGGCCTGTCATTCGCGGCAGCGGTTGATCCGATCCAGATCCCAATCCGACAGCCCGTCACTGCGC
GCCACGCGGTTATCGAGATC

Product: flavin reductase-like FMN-binding protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MDDLKATFLEGMSRAAASVCVVTTDGPAGRAGVTVSAMTSISADGEAPTMLTCLNASSSCLPILLENACFCINVLSTGQT
EISDVFSSRIPAPGGDKFNAVAHTVLASGAPYLSDALVGFDCTLVSAEKLGTHHICIGAVQAVRVAPEGEPLLYGMRKYL
RAETH

Sequences:

>Translated_165_residues
MDDLKATFLEGMSRAAASVCVVTTDGPAGRAGVTVSAMTSISADGEAPTMLTCLNASSSCLPILLENACFCINVLSTGQT
EISDVFSSRIPAPGGDKFNAVAHTVLASGAPYLSDALVGFDCTLVSAEKLGTHHICIGAVQAVRVAPEGEPLLYGMRKYL
RAETH
>Mature_165_residues
MDDLKATFLEGMSRAAASVCVVTTDGPAGRAGVTVSAMTSISADGEAPTMLTCLNASSSCLPILLENACFCINVLSTGQT
EISDVFSSRIPAPGGDKFNAVAHTVLASGAPYLSDALVGFDCTLVSAEKLGTHHICIGAVQAVRVAPEGEPLLYGMRKYL
RAETH

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=155, Percent_Identity=38.0645161290323, Blast_Score=105, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 17037; Mature: 17037

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDLKATFLEGMSRAAASVCVVTTDGPAGRAGVTVSAMTSISADGEAPTMLTCLNASSSC
CCCHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEHEEECCCCCCCCEEEEEECCCCCH
LPILLENACFCINVLSTGQTEISDVFSSRIPAPGGDKFNAVAHTVLASGAPYLSDALVGF
HHHHHHCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCC
DCTLVSAEKLGTHHICIGAVQAVRVAPEGEPLLYGMRKYLRAETH
CEEEEEHHHCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDDLKATFLEGMSRAAASVCVVTTDGPAGRAGVTVSAMTSISADGEAPTMLTCLNASSSC
CCCHHHHHHHHHHHHHCEEEEEECCCCCCCCCEEEEHEEECCCCCCCCEEEEEECCCCCH
LPILLENACFCINVLSTGQTEISDVFSSRIPAPGGDKFNAVAHTVLASGAPYLSDALVGF
HHHHHHCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCC
DCTLVSAEKLGTHHICIGAVQAVRVAPEGEPLLYGMRKYLRAETH
CEEEEEHHHCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA