Click here to switch to the map view.

The map label for this gene is bcr [H]

Identifier: 89054809

GI number: 89054809

Start: 2321785

End: 2323002

Strand: Direct

Name: bcr [H]

Synonym: Jann_2318

Alternate gene names: 89054809

Gene position: 2321785-2323002 (Clockwise)

Preceding gene: 89054808

Following gene: 89054810

Centisome position: 53.77

GC content: 65.35

Gene sequence:

>1218_bases
ATGACTGACACCACACCCGCTGCCCGCACGCGCCTGTCGACCGGCGAATTCATTGCCCTGATCGGCATGATGTTCGGCAC
GATTGCATTCTCCATTGATGCGATGTTGCCCGCCCTGCCCCAGATCGCGGAAGAACTGACGCCCGATGATCTGACCCTCG
CGCAGTTGGTGCTGACCAGTTTCATCCTGGGAATGGGCGTTGGAACGCTGTTTACCGGGCCCTTGTCCGATGCGTTCGGG
CGCAAGCCGGTGATCTTTGCGGGCGCGATCCTTTATATCGCAGGCTCGATCCTCGCCTGGGCCGCGCCGACGCTGGAATT
GCTGCTGGCCGCCCGTGTCTTGCAGGGGCTGGGGGCCGCCGGGCCGCGCGTCGTGGCGATGGCGTTGGTGCGCGACCTTT
ATACAGGCCGGGCCATGGCGCGGATCATGTCCTATGCGATGCTGGTCTTCACCATCACGCCCGCCATTGCGCCGCTGATC
GGCGAGGGGATCATCACCCTGTTCGACTGGCGCGCGATCTTTCTGACCTATGTCCTGTTCTCCTGCCTCACCGTGGGTTG
GCTGATGCTGCGCCAGCCCGAGACCCTGCCGCGCGGCCTGCGCAAACGGCTGAACCTGTCCTCTCTCTGGGCCGCAACGC
GCGAGGCGATGGCCCTGCGTCAGATGCAGCTGTCGATCCTGGTGCAATCGCTGATTTACGGTGCGCTGTTCGGGACGCTG
TCGTCGGTCCAGCAGATCTTTGATCTGACCTATGACATGGGCGATGCCTTCCCCTGGCTGTTCGGGGCCGTGGCCCTTCT
GGCCGCGCCCGGCGCGCCGATCAACGGACGCCTTGTGGTGCGGTTGGGGATGCGCCCCCTCGTGCGCCGAGCGCTGTTTT
TGCAGGTGGCGGCGGCCGCGCTCTTCGTGTCTGCCCTGATGCTTGGCCTCCCCCCCGGCACCGAGGTCTGGATCTACTTC
GCCTGGACCGTGACGGTCTTCATGATGATGGGCTTCACCATCGGCAACCTCAACGCCCTGGCGCTGGAGCCTCTGGGCCA
TATCGCGGGCATCTCGGCCTCGTTGATGGGGTCCATTTCAACCATTGGAGGGGCCGCGATTGGTGCGCTGATCGGGCAGC
TTTATGACGGGACCGCCCTGCCCCTGGCCGTGGCAACCGCGCTTCTGGCCGGGATCGGCGGGCTGATCATGCGCCGGATG
CCGCGGGAGGGGCGGTAG

Upstream 100 bases:

>100_bases
GGCGTGCCCTGGGTTCTGGCATTTCTGACCCATTGGTCGATCGTCTTTGAGTTGATGATCTGGGCCGTCGCGGCGGCCTT
CTGGATAAGGCGACGTCAGC

Downstream 100 bases:

>100_bases
GTGCACGTGTCACTGTTTGGAGAATGGGTCGGGAGCGGCGTCGATGTCACAAAGTGGAAGCAAGATAATCCTGTTTCATC
ACACATCGCTGGTTACGATC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MTDTTPAARTRLSTGEFIALIGMMFGTIAFSIDAMLPALPQIAEELTPDDLTLAQLVLTSFILGMGVGTLFTGPLSDAFG
RKPVIFAGAILYIAGSILAWAAPTLELLLAARVLQGLGAAGPRVVAMALVRDLYTGRAMARIMSYAMLVFTITPAIAPLI
GEGIITLFDWRAIFLTYVLFSCLTVGWLMLRQPETLPRGLRKRLNLSSLWAATREAMALRQMQLSILVQSLIYGALFGTL
SSVQQIFDLTYDMGDAFPWLFGAVALLAAPGAPINGRLVVRLGMRPLVRRALFLQVAAAALFVSALMLGLPPGTEVWIYF
AWTVTVFMMMGFTIGNLNALALEPLGHIAGISASLMGSISTIGGAAIGALIGQLYDGTALPLAVATALLAGIGGLIMRRM
PREGR

Sequences:

>Translated_405_residues
MTDTTPAARTRLSTGEFIALIGMMFGTIAFSIDAMLPALPQIAEELTPDDLTLAQLVLTSFILGMGVGTLFTGPLSDAFG
RKPVIFAGAILYIAGSILAWAAPTLELLLAARVLQGLGAAGPRVVAMALVRDLYTGRAMARIMSYAMLVFTITPAIAPLI
GEGIITLFDWRAIFLTYVLFSCLTVGWLMLRQPETLPRGLRKRLNLSSLWAATREAMALRQMQLSILVQSLIYGALFGTL
SSVQQIFDLTYDMGDAFPWLFGAVALLAAPGAPINGRLVVRLGMRPLVRRALFLQVAAAALFVSALMLGLPPGTEVWIYF
AWTVTVFMMMGFTIGNLNALALEPLGHIAGISASLMGSISTIGGAAIGALIGQLYDGTALPLAVATALLAGIGGLIMRRM
PREGR
>Mature_404_residues
TDTTPAARTRLSTGEFIALIGMMFGTIAFSIDAMLPALPQIAEELTPDDLTLAQLVLTSFILGMGVGTLFTGPLSDAFGR
KPVIFAGAILYIAGSILAWAAPTLELLLAARVLQGLGAAGPRVVAMALVRDLYTGRAMARIMSYAMLVFTITPAIAPLIG
EGIITLFDWRAIFLTYVLFSCLTVGWLMLRQPETLPRGLRKRLNLSSLWAATREAMALRQMQLSILVQSLIYGALFGTLS
SVQQIFDLTYDMGDAFPWLFGAVALLAAPGAPINGRLVVRLGMRPLVRRALFLQVAAAALFVSALMLGLPPGTEVWIYFA
WTVTVFMMMGFTIGNLNALALEPLGHIAGISASLMGSISTIGGAAIGALIGQLYDGTALPLAVATALLAGIGGLIMRRMP
REGR

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=384, Percent_Identity=29.9479166666667, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790146, Length=143, Percent_Identity=38.4615384615385, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994889, Length=145, Percent_Identity=36.551724137931, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082312, Length=163, Percent_Identity=34.9693251533742, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789312, Length=121, Percent_Identity=37.1900826446281, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=161, Percent_Identity=27.9503105590062, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43081; Mature: 42950

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTTPAARTRLSTGEFIALIGMMFGTIAFSIDAMLPALPQIAEELTPDDLTLAQLVLTS
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FILGMGVGTLFTGPLSDAFGRKPVIFAGAILYIAGSILAWAAPTLELLLAARVLQGLGAA
HHHHHCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GPRVVAMALVRDLYTGRAMARIMSYAMLVFTITPAIAPLIGEGIITLFDWRAIFLTYVLF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SCLTVGWLMLRQPETLPRGLRKRLNLSSLWAATREAMALRQMQLSILVQSLIYGALFGTL
HHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVQQIFDLTYDMGDAFPWLFGAVALLAAPGAPINGRLVVRLGMRPLVRRALFLQVAAAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
LFVSALMLGLPPGTEVWIYFAWTVTVFMMMGFTIGNLNALALEPLGHIAGISASLMGSIS
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TIGGAAIGALIGQLYDGTALPLAVATALLAGIGGLIMRRMPREGR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TDTTPAARTRLSTGEFIALIGMMFGTIAFSIDAMLPALPQIAEELTPDDLTLAQLVLTS
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FILGMGVGTLFTGPLSDAFGRKPVIFAGAILYIAGSILAWAAPTLELLLAARVLQGLGAA
HHHHHCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GPRVVAMALVRDLYTGRAMARIMSYAMLVFTITPAIAPLIGEGIITLFDWRAIFLTYVLF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SCLTVGWLMLRQPETLPRGLRKRLNLSSLWAATREAMALRQMQLSILVQSLIYGALFGTL
HHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVQQIFDLTYDMGDAFPWLFGAVALLAAPGAPINGRLVVRLGMRPLVRRALFLQVAAAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
LFVSALMLGLPPGTEVWIYFAWTVTVFMMMGFTIGNLNALALEPLGHIAGISASLMGSIS
HHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TIGGAAIGALIGQLYDGTALPLAVATALLAGIGGLIMRRMPREGR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]