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The map label for this gene is amtB [C]

Identifier: 89054723

GI number: 89054723

Start: 2237171

End: 2238355

Strand: Reverse

Name: amtB [C]

Synonym: Jann_2232

Alternate gene names: 89054723

Gene position: 2238355-2237171 (Counterclockwise)

Preceding gene: 89054724

Following gene: 89054721

Centisome position: 51.84

GC content: 60.93

Gene sequence:

>1185_bases
ATGAACGGAGCGGACACCGCATGGATCATCGTCGCCACGGCGCTGGTCCTCTTTATGTCATTGCCAGGACTGGCGTTATT
TTACGGCGGCCTGGTGCGCGCACGAAACGTGTTGAGCGTCTTCATGCACGTCTATGCGATTGCCGCATTGATGAGCCTCC
TGTGGCTCGCGGTCGGGTATTCCATCGCGTTTGGGCCGGGTGACAGCGGCTATTGGGGCGGGCTGGAGCGCGCATTTCTG
ATCGGTGTAACCGCCGAAACGCTATCGGGCACCCTGCCCGAGGTGTTGTTTTTCGCCTTCCAGATGACCTTCGCGATCAT
CACGCCCGCGCTGATCGTGGGCGCATATGTTGAACGGATCGGCTTCGGCTTCGTTCTGTTGTTTTCCGCGCTTTGGATGC
TGATTGTCTACGCACCCGTCGTGCATTGGATCTGGGGCGGAGGCATGTTGTCGGATGGCGGCCTTTTGGGTGAAATTGGC
GTGCGCGACTTCGCGGGCGGCATCGTGGTCCATGAGACGGCAGGTCTGGCTGCACTGGTTCTTGCGGTGATGTTGGGCGC
ACGCAAGAACAACACCACGCCGCCCCACAATCCCGGCATGGTGATGATCGGCGCGGCGATGCTGTGGGTCGGTTGGTTCG
GCTTCAATGGCGGCAGCCAGCTGGCCGCGGATGGCGGGGCCGCGATGGCGCTGACCGTCACCCATATCTCGGCCGCGGCG
GCAACGCTGACCTGGGGACTTTGGGAGAAGGTCAAATACGGCAAGGTGTCTCTCGTGGGCATCGTGACGGGCACGATTGC
GGGGCTCGCCTCGATCACACCGGCCTCCGGCTTTGTCGGACCCGTGGCTGCGCTAATCATCGGCTCAGTCGCGGGCATCC
TGTGCCAGGAAGCGGTGAACCTGATCCGCAACAAAGCACGGATCGACGACACGCTGGACGTGTTTGCCGTCCACGGCGTC
GGCGGCATTTTCGGGACGATCATGATTATTGTATTCGGAACCGACGTCAGCGCAGTCGCACAATTCGGCGGTCTGCTGAT
CGTGGGCGTCTATACGGTTGTTTTGACGGTCATCATTGCCAAGGTCGTGGGTCTGATCACACCTCTTCGGGTCGATGAAG
AGGCCGAGGTGACGGGACTGAATATCACCGTTCACGGCGAGCGGGCCTATGACATGAGCTCGTAG

Upstream 100 bases:

>100_bases
CAAATCGCCCACATTTTGACCATGCAATTCACCTGCCGCCTCTTTTTTCCACGGTCCCAACCCGCTTTGTCAGCCCCAAT
CAAGGGAGAGGGACATCCAA

Downstream 100 bases:

>100_bases
ACGTTAAGTCCGTTAAGACCTTGCACAAACGCCCGGGCCATGGCTCGGGCGTCTTGCGTGTGCCACGGGCTATGTGATCT
TGCGTGGCAATGGAAACGGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNGADTAWIIVATALVLFMSLPGLALFYGGLVRARNVLSVFMHVYAIAALMSLLWLAVGYSIAFGPGDSGYWGGLERAFL
IGVTAETLSGTLPEVLFFAFQMTFAIITPALIVGAYVERIGFGFVLLFSALWMLIVYAPVVHWIWGGGMLSDGGLLGEIG
VRDFAGGIVVHETAGLAALVLAVMLGARKNNTTPPHNPGMVMIGAAMLWVGWFGFNGGSQLAADGGAAMALTVTHISAAA
ATLTWGLWEKVKYGKVSLVGIVTGTIAGLASITPASGFVGPVAALIIGSVAGILCQEAVNLIRNKARIDDTLDVFAVHGV
GGIFGTIMIIVFGTDVSAVAQFGGLLIVGVYTVVLTVIIAKVVGLITPLRVDEEAEVTGLNITVHGERAYDMSS

Sequences:

>Translated_394_residues
MNGADTAWIIVATALVLFMSLPGLALFYGGLVRARNVLSVFMHVYAIAALMSLLWLAVGYSIAFGPGDSGYWGGLERAFL
IGVTAETLSGTLPEVLFFAFQMTFAIITPALIVGAYVERIGFGFVLLFSALWMLIVYAPVVHWIWGGGMLSDGGLLGEIG
VRDFAGGIVVHETAGLAALVLAVMLGARKNNTTPPHNPGMVMIGAAMLWVGWFGFNGGSQLAADGGAAMALTVTHISAAA
ATLTWGLWEKVKYGKVSLVGIVTGTIAGLASITPASGFVGPVAALIIGSVAGILCQEAVNLIRNKARIDDTLDVFAVHGV
GGIFGTIMIIVFGTDVSAVAQFGGLLIVGVYTVVLTVIIAKVVGLITPLRVDEEAEVTGLNITVHGERAYDMSS
>Mature_394_residues
MNGADTAWIIVATALVLFMSLPGLALFYGGLVRARNVLSVFMHVYAIAALMSLLWLAVGYSIAFGPGDSGYWGGLERAFL
IGVTAETLSGTLPEVLFFAFQMTFAIITPALIVGAYVERIGFGFVLLFSALWMLIVYAPVVHWIWGGGMLSDGGLLGEIG
VRDFAGGIVVHETAGLAALVLAVMLGARKNNTTPPHNPGMVMIGAAMLWVGWFGFNGGSQLAADGGAAMALTVTHISAAA
ATLTWGLWEKVKYGKVSLVGIVTGTIAGLASITPASGFVGPVAALIIGSVAGILCQEAVNLIRNKARIDDTLDVFAVHGV
GGIFGTIMIIVFGTDVSAVAQFGGLLIVGVYTVVLTVIIAKVVGLITPLRVDEEAEVTGLNITVHGERAYDMSS

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=407, Percent_Identity=42.2604422604423, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17550182, Length=435, Percent_Identity=26.4367816091954, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17550180, Length=431, Percent_Identity=26.9141531322506, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI32564042, Length=421, Percent_Identity=25.6532066508314, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI133931100, Length=429, Percent_Identity=23.5431235431235, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324187, Length=426, Percent_Identity=34.9765258215962, Blast_Score=199, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6325396, Length=419, Percent_Identity=33.6515513126492, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=32.5358851674641, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI161078314, Length=415, Percent_Identity=27.2289156626506, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047
- InterPro:   IPR002229 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 40962; Mature: 40962

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGADTAWIIVATALVLFMSLPGLALFYGGLVRARNVLSVFMHVYAIAALMSLLWLAVGY
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIAFGPGDSGYWGGLERAFLIGVTAETLSGTLPEVLFFAFQMTFAIITPALIVGAYVERI
EEEECCCCCCCCCCCCHHEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFGFVLLFSALWMLIVYAPVVHWIWGGGMLSDGGLLGEIGVRDFAGGIVVHETAGLAALV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEECCCHHHHCCCEEEECCCCHHHHH
LAVMLGARKNNTTPPHNPGMVMIGAAMLWVGWFGFNGGSQLAADGGAAMALTVTHISAAA
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHH
ATLTWGLWEKVKYGKVSLVGIVTGTIAGLASITPASGFVGPVAALIIGSVAGILCQEAVN
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIRNKARIDDTLDVFAVHGVGGIFGTIMIIVFGTDVSAVAQFGGLLIVGVYTVVLTVIIA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHH
KVVGLITPLRVDEEAEVTGLNITVHGERAYDMSS
HHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MNGADTAWIIVATALVLFMSLPGLALFYGGLVRARNVLSVFMHVYAIAALMSLLWLAVGY
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIAFGPGDSGYWGGLERAFLIGVTAETLSGTLPEVLFFAFQMTFAIITPALIVGAYVERI
EEEECCCCCCCCCCCCHHEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFGFVLLFSALWMLIVYAPVVHWIWGGGMLSDGGLLGEIGVRDFAGGIVVHETAGLAALV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEECCCHHHHCCCEEEECCCCHHHHH
LAVMLGARKNNTTPPHNPGMVMIGAAMLWVGWFGFNGGSQLAADGGAAMALTVTHISAAA
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHH
ATLTWGLWEKVKYGKVSLVGIVTGTIAGLASITPASGFVGPVAALIIGSVAGILCQEAVN
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIRNKARIDDTLDVFAVHGVGGIFGTIMIIVFGTDVSAVAQFGGLLIVGVYTVVLTVIIA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHH
KVVGLITPLRVDEEAEVTGLNITVHGERAYDMSS
HHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]