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The map label for this gene is metZ [H]

Identifier: 89054345

GI number: 89054345

Start: 1841576

End: 1842769

Strand: Direct

Name: metZ [H]

Synonym: Jann_1854

Alternate gene names: 89054345

Gene position: 1841576-1842769 (Clockwise)

Preceding gene: 89054339

Following gene: 89054346

Centisome position: 42.65

GC content: 63.9

Gene sequence:

>1194_bases
ATGAAAGATACCGCTCGCAAACACTGGTCCAAACGCACCCGCGCGGTGCATGCAGGCTCCCGCCGCAGCCAATACGGAGA
GCTGTCGGAAGCGATGTTCCTGACGCAGGGCTTCGTCTATCCCACCGCCGAGGATGCGGAGGCCCGTTTCATCAAATCCG
GCGAGGATGAGTATATCTACGCCCGCTACGGCAACCCGACCGTGGCGATGTTTGAAGACCGCATCGCGTCGCTGGAAGGG
GCGGAGGCGGGCTTTGCCACGGCCTCGGGCATGGCGGCAGTCAATGGCGCGCTCACGTCGATGCTGCGGGCGGGCGATCA
CGTGGTGTCGTCCCGCGCGCTTTTTGGGTCGTGTCATTATGTCTTGGACGAGATCCTGACCCGGTTTGGCGTGGACGTCA
CCTTCGTGGACGGCCCCGATCTGGACGCGTGGCGCGCCGCCATGCGCCCGGACACCAAGGCGGTGTTCTTCGAATCGCTC
TCCAATCCCACGCTGGAGATGATCGACATTCGCGCCGTGGCTGAGATCGCCCATGCCGTCGGTGCGACGGTCATCTGCGA
TAACGTCTTTGCCACCCCCACGTTCAGCGATGCCATCGCCCAAGGCGTCGATGTCGTTGTCTATTCCACCACCAAACACA
TTGACGGGCAGGGGCGCTGTCTGGGGGGCGTGATCCTGGGGACGGAAGAATTTATCCGCAAAACGGTGGAGCCTTACCTC
AAGCACACCGGCGGCGCGATGTCGCCCTTCAACGCGTGGGTGATGCTGAAGGGGCTGGAGACGATGGACCTGCGGGTGCG
GGCGCAAACTGCGTCGGCTCAGGCGATTGCGGAGGCGCTGCAAGATGCGCCCGGTGTGGCGCGGGTGATTTATCCCGGCC
TCGCCGACCACCCCCAGCACGCGCTCTGCCAGGCGCAGATGGGCGAGGGGGGGACCGTCGTTGCGGTGGAGGCCACGGAT
GGACAGGCGGGGGCGTTCCGCGCGCTCAATGCGCTGGAGATCTTCACGATTTCCAACAATCTTGGCGATGCGAAGTCCAT
TGCCACCCATCCCACGACGACCACCCACCAGCGCCTGACCGATGAGCAGCGCGCGGAGATGGGGATCACGCCGGGCCTGA
TCCGTCTGTCGATCGGCTTGGAAGACACCGATGATCTGGTCGCAGACCTGCTTGATGCGTTGGAACTGGCATGA

Upstream 100 bases:

>100_bases
TACCGCTAAAGAGGTCGAGGCCGCTGAAAGACAGCGCCGTCCCCGACCATGACCTTGGGCAATCGCACGACGCGGTGCCC
CGCAGAATGGAGATAGGGGT

Downstream 100 bases:

>100_bases
ACGGTGTGATCATTCGCCCCTATCGCCCGGCGGACGCGATCCCCCTGGCCACGCTCTACCACCGCGCAGTGAATGAGGGG
GCGGCGTTGAAATATGATGC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKDTARKHWSKRTRAVHAGSRRSQYGELSEAMFLTQGFVYPTAEDAEARFIKSGEDEYIYARYGNPTVAMFEDRIASLEG
AEAGFATASGMAAVNGALTSMLRAGDHVVSSRALFGSCHYVLDEILTRFGVDVTFVDGPDLDAWRAAMRPDTKAVFFESL
SNPTLEMIDIRAVAEIAHAVGATVICDNVFATPTFSDAIAQGVDVVVYSTTKHIDGQGRCLGGVILGTEEFIRKTVEPYL
KHTGGAMSPFNAWVMLKGLETMDLRVRAQTASAQAIAEALQDAPGVARVIYPGLADHPQHALCQAQMGEGGTVVAVEATD
GQAGAFRALNALEIFTISNNLGDAKSIATHPTTTTHQRLTDEQRAEMGITPGLIRLSIGLEDTDDLVADLLDALELA

Sequences:

>Translated_397_residues
MKDTARKHWSKRTRAVHAGSRRSQYGELSEAMFLTQGFVYPTAEDAEARFIKSGEDEYIYARYGNPTVAMFEDRIASLEG
AEAGFATASGMAAVNGALTSMLRAGDHVVSSRALFGSCHYVLDEILTRFGVDVTFVDGPDLDAWRAAMRPDTKAVFFESL
SNPTLEMIDIRAVAEIAHAVGATVICDNVFATPTFSDAIAQGVDVVVYSTTKHIDGQGRCLGGVILGTEEFIRKTVEPYL
KHTGGAMSPFNAWVMLKGLETMDLRVRAQTASAQAIAEALQDAPGVARVIYPGLADHPQHALCQAQMGEGGTVVAVEATD
GQAGAFRALNALEIFTISNNLGDAKSIATHPTTTTHQRLTDEQRAEMGITPGLIRLSIGLEDTDDLVADLLDALELA
>Mature_397_residues
MKDTARKHWSKRTRAVHAGSRRSQYGELSEAMFLTQGFVYPTAEDAEARFIKSGEDEYIYARYGNPTVAMFEDRIASLEG
AEAGFATASGMAAVNGALTSMLRAGDHVVSSRALFGSCHYVLDEILTRFGVDVTFVDGPDLDAWRAAMRPDTKAVFFESL
SNPTLEMIDIRAVAEIAHAVGATVICDNVFATPTFSDAIAQGVDVVVYSTTKHIDGQGRCLGGVILGTEEFIRKTVEPYL
KHTGGAMSPFNAWVMLKGLETMDLRVRAQTASAQAIAEALQDAPGVARVIYPGLADHPQHALCQAQMGEGGTVVAVEATD
GQAGAFRALNALEIFTISNNLGDAKSIATHPTTTTHQRLTDEQRAEMGITPGLIRLSIGLEDTDDLVADLLDALELA

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=372, Percent_Identity=31.7204301075269, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI299473759, Length=372, Percent_Identity=28.7634408602151, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI25453487, Length=371, Percent_Identity=27.2237196765499, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1790375, Length=372, Percent_Identity=31.4516129032258, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1789383, Length=390, Percent_Identity=26.6666666666667, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560056, Length=391, Percent_Identity=35.2941176470588, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17538055, Length=390, Percent_Identity=35.3846153846154, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=407, Percent_Identity=29.7297297297297, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=393, Percent_Identity=33.8422391857506, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=383, Percent_Identity=30.0261096605744, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=24.8768472906404, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=277, Percent_Identity=29.2418772563177, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=401, Percent_Identity=24.9376558603491, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922554, Length=390, Percent_Identity=33.8461538461538, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42603; Mature: 42603

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTARKHWSKRTRAVHAGSRRSQYGELSEAMFLTQGFVYPTAEDAEARFIKSGEDEYIY
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCEEE
ARYGNPTVAMFEDRIASLEGAEAGFATASGMAAVNGALTSMLRAGDHVVSSRALFGSCHY
EEECCCEEEEHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VLDEILTRFGVDVTFVDGPDLDAWRAAMRPDTKAVFFESLSNPTLEMIDIRAVAEIAHAV
HHHHHHHHHCCEEEEECCCCHHHHHHHCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHH
GATVICDNVFATPTFSDAIAQGVDVVVYSTTKHIDGQGRCLGGVILGTEEFIRKTVEPYL
CCCEEECCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHH
KHTGGAMSPFNAWVMLKGLETMDLRVRAQTASAQAIAEALQDAPGVARVIYPGLADHPQH
HHCCCCCCCCCEEEEEECCCEEEEEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHH
ALCQAQMGEGGTVVAVEATDGQAGAFRALNALEIFTISNNLGDAKSIATHPTTTTHQRLT
HHHHHHCCCCCEEEEEEECCCCCCHHHHCCEEEEEEEECCCCCCHHCCCCCCCCHHHHHC
DEQRAEMGITPGLIRLSIGLEDTDDLVADLLDALELA
HHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDTARKHWSKRTRAVHAGSRRSQYGELSEAMFLTQGFVYPTAEDAEARFIKSGEDEYIY
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCEEE
ARYGNPTVAMFEDRIASLEGAEAGFATASGMAAVNGALTSMLRAGDHVVSSRALFGSCHY
EEECCCEEEEHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VLDEILTRFGVDVTFVDGPDLDAWRAAMRPDTKAVFFESLSNPTLEMIDIRAVAEIAHAV
HHHHHHHHHCCEEEEECCCCHHHHHHHCCCCCHHHHHHHCCCCCEEHHHHHHHHHHHHHH
GATVICDNVFATPTFSDAIAQGVDVVVYSTTKHIDGQGRCLGGVILGTEEFIRKTVEPYL
CCCEEECCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHH
KHTGGAMSPFNAWVMLKGLETMDLRVRAQTASAQAIAEALQDAPGVARVIYPGLADHPQH
HHCCCCCCCCCEEEEEECCCEEEEEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHH
ALCQAQMGEGGTVVAVEATDGQAGAFRALNALEIFTISNNLGDAKSIATHPTTTTHQRLT
HHHHHHCCCCCEEEEEEECCCCCCHHHHCCEEEEEEEECCCCCCHHCCCCCCCCHHHHHC
DEQRAEMGITPGLIRLSIGLEDTDDLVADLLDALELA
HHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]