Click here to switch to the map view.

The map label for this gene is cefE [H]

Identifier: 89054225

GI number: 89054225

Start: 1727223

End: 1728143

Strand: Direct

Name: cefE [H]

Synonym: Jann_1734

Alternate gene names: 89054225

Gene position: 1727223-1728143 (Clockwise)

Preceding gene: 89054223

Following gene: 89054234

Centisome position: 40.0

GC content: 64.39

Gene sequence:

>921_bases
ATGATCCCGCATATCGACGCAAACGCCCTCGCCGCCCGTGACCCGGACGCCCTGGCCGCCACCAAGATCGGGGTGGAGGA
TGTGGGCTTCCTGACCCTTCACAACACGGCGCTGACGGCGGATCAGGTGCGTGCCACGCTTGCCGCATATCGCACCTTCT
TCAAAGGCGATGTCGCCAACAAGCAGGCGGTGAACATGAATGTCACAGGCTCCAATCGCGGCTGGGGCGGGGCGGGGTCC
GAACAGGTCGATCCTGACGCCAACGCGGACTACAAGGAGGTCTTCGATTGCGGGGTGGAGCTGCCCAAGGACGATCCCTT
CGCGCGGTTGCCCGTCTATGCGCCCAACCAATGGCCCGCGCGGCCCGACGGGTTTCAGCAGGCGGTGGAGGGGTATTTCA
CCGCCGCCCGCGTGGTGTGTCTGGACCTGTTGCAAGGCATTTCCGCGGCCATCGGCGCGGATCCCACCTATTTCGCGGAC
AAGTTCGCCAAGCCGATGGCGCTTCTGCGCGGCAATTATTACCCGGAACGGCCCGATTGGGCGGGGGAGAAGGATTTCGG
CATCGCGGCGCACACTGATTACGGCTGCCTGACGCTTTTGGCGACCGATGGCCAGCCGGGTCTTGAGGTGCAGATGCGCG
ACGGGGCGTGGGTGCCGGTGCAGGCCGAGCCGGGCACCTTCATCATCAATTTCGGTGAAATGCTGGAGATGTGGACCGCC
AATCCCGACGGCGGTGGCCAGATCCGCGCCACGCCGCACCGGGTGGTGGGCGGCGCCCATGAGCGGATCTCCATCCCGCT
ATTCTTCAACCCGGTCTATGATACCAATGTCACGCCGCCGGGCACGCCACGCCTGAGTGCGGGCGAGCACCTCTCAAGAC
GGTATCAGGAAACGTATACGCATTTGCAGGACACGGAGTGA

Upstream 100 bases:

>100_bases
TTGCCTGCCGCCAGTTTCCGTGCCATCGCCGCCTCCAGAGTTCATTTGTTCCCGTGGTCGCACCCACGGCACAGGCGGTC
AACCCGACAGGAGGTCCCCG

Downstream 100 bases:

>100_bases
TTACAGCAGCTGGCGGTAGAGGATGATGGCGCTGGCGAGCAGGATGGTCAGGCCAATGCGAGGGCGATCCGGGCCTGCAT
TGTCGCGCGCCTCGGTCAGG

Product: 2OG-Fe(II) oxygenase

Products: NA

Alternate protein names: DAOCS; Expandase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MIPHIDANALAARDPDALAATKIGVEDVGFLTLHNTALTADQVRATLAAYRTFFKGDVANKQAVNMNVTGSNRGWGGAGS
EQVDPDANADYKEVFDCGVELPKDDPFARLPVYAPNQWPARPDGFQQAVEGYFTAARVVCLDLLQGISAAIGADPTYFAD
KFAKPMALLRGNYYPERPDWAGEKDFGIAAHTDYGCLTLLATDGQPGLEVQMRDGAWVPVQAEPGTFIINFGEMLEMWTA
NPDGGGQIRATPHRVVGGAHERISIPLFFNPVYDTNVTPPGTPRLSAGEHLSRRYQETYTHLQDTE

Sequences:

>Translated_306_residues
MIPHIDANALAARDPDALAATKIGVEDVGFLTLHNTALTADQVRATLAAYRTFFKGDVANKQAVNMNVTGSNRGWGGAGS
EQVDPDANADYKEVFDCGVELPKDDPFARLPVYAPNQWPARPDGFQQAVEGYFTAARVVCLDLLQGISAAIGADPTYFAD
KFAKPMALLRGNYYPERPDWAGEKDFGIAAHTDYGCLTLLATDGQPGLEVQMRDGAWVPVQAEPGTFIINFGEMLEMWTA
NPDGGGQIRATPHRVVGGAHERISIPLFFNPVYDTNVTPPGTPRLSAGEHLSRRYQETYTHLQDTE
>Mature_306_residues
MIPHIDANALAARDPDALAATKIGVEDVGFLTLHNTALTADQVRATLAAYRTFFKGDVANKQAVNMNVTGSNRGWGGAGS
EQVDPDANADYKEVFDCGVELPKDDPFARLPVYAPNQWPARPDGFQQAVEGYFTAARVVCLDLLQGISAAIGADPTYFAD
KFAKPMALLRGNYYPERPDWAGEKDFGIAAHTDYGCLTLLATDGQPGLEVQMRDGAWVPVQAEPGTFIINFGEMLEMWTA
NPDGGGQIRATPHRVVGGAHERISIPLFFNPVYDTNVTPPGTPRLSAGEHLSRRYQETYTHLQDTE

Specific function: Catalyzes the step from penicillin N to deacetoxy- cephalosporin C [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI28572124, Length=282, Percent_Identity=28.3687943262411, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.14.20.1 [H]

Molecular weight: Translated: 33256; Mature: 33256

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPHIDANALAARDPDALAATKIGVEDVGFLTLHNTALTADQVRATLAAYRTFFKGDVAN
CCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCC
KQAVNMNVTGSNRGWGGAGSEQVDPDANADYKEVFDCGVELPKDDPFARLPVYAPNQWPA
CEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEECCEECCCCCCC
RPDGFQQAVEGYFTAARVVCLDLLQGISAAIGADPTYFADKFAKPMALLRGNYYPERPDW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
AGEKDFGIAAHTDYGCLTLLATDGQPGLEVQMRDGAWVPVQAEPGTFIINFGEMLEMWTA
CCCCCCCEEEECCCCEEEEEEECCCCCCEEEECCCCEEEEECCCCEEEEEHHHHHHHHCC
NPDGGGQIRATPHRVVGGAHERISIPLFFNPVYDTNVTPPGTPRLSAGEHLSRRYQETYT
CCCCCCEEEECCHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLQDTE
HHCCCC
>Mature Secondary Structure
MIPHIDANALAARDPDALAATKIGVEDVGFLTLHNTALTADQVRATLAAYRTFFKGDVAN
CCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCC
KQAVNMNVTGSNRGWGGAGSEQVDPDANADYKEVFDCGVELPKDDPFARLPVYAPNQWPA
CEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEECCEECCCCCCC
RPDGFQQAVEGYFTAARVVCLDLLQGISAAIGADPTYFADKFAKPMALLRGNYYPERPDW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
AGEKDFGIAAHTDYGCLTLLATDGQPGLEVQMRDGAWVPVQAEPGTFIINFGEMLEMWTA
CCCCCCCEEEECCCCEEEEEEECCCCCCEEEECCCCEEEEECCCCEEEEEHHHHHHHHCC
NPDGGGQIRATPHRVVGGAHERISIPLFFNPVYDTNVTPPGTPRLSAGEHLSRRYQETYT
CCCCCCEEEECCHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLQDTE
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8437592 [H]