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The map label for this gene is gph [C]

Identifier: 89054149

GI number: 89054149

Start: 1641969

End: 1642655

Strand: Direct

Name: gph [C]

Synonym: Jann_1658

Alternate gene names: 89054149

Gene position: 1641969-1642655 (Clockwise)

Preceding gene: 89054148

Following gene: 89054150

Centisome position: 38.03

GC content: 65.65

Gene sequence:

>687_bases
ATGACCAAAATGACCACATGTGTGTTTGATGCTTATGGCACGCTCTTTGATGTCGCCGCGGCGGCCCGCGAATTGGCGGA
GCGGCCCGGGCGGGAGGCGTTTGCGGAGGTCTGGCAGCAGGTGGCGAATGACTGGCGACTGAAGCAGTTGCAGTATTCGT
GGCTGCGGGCCGTGGCGCGGGACCATTGCGATTTCTGGAGTGTCACCGGTGATGGCTTGGATTGGGCGCTGGAGAGGGCG
GGGCTGGCGGATATGGAACTGCGCGAAGAGTTGATGGGGCTGTATTTCACTCTGTCCGCCTATCCGGAGGTGCCGGAGAT
GTTGGGCCGCCTCAAGGCTGCGGGGCTGCAGACGGGGATCTTGTCCAACGGCTCGCCAGATATGCTGCGCGGTGCGGTGG
AAAGCGCGGGCCTGAGCGACCTGCTGGATGCCGTGTTGAGTGTGGAGGATGTGGGCGTCTTCAAGCCCGACATGCGGGTC
TACGACATGATCGGGCAGCGGTTCGGCTGCGCGCCGGGCGACGTTCTGTTCGTGTCGGCCAATGGCTGGGACGCGGCGTT
CGGGGCGGCCTATGGGTTCGACACGCTCTGGGTGAACCGGGGCGGGGAGCCGATGGATCGGCTGACGGGCAAACCGGGGC
GCGAGGCGCGCGATCTGACCGGCGTGGCCGCGCTTGTGGGGGCGTGA

Upstream 100 bases:

>100_bases
ATCGCAACCCCCCGGGATATTTGAAGAACGGGGAAGGAGGGGCGTTGCGGCTTGCCCTGAGGCATGGTTCCTTTGCGCTG
GACGCCGGGGAGGGCTTGCG

Downstream 100 bases:

>100_bases
GCGATGGCATTTGTGACGGCAGCTGATGGCGTGCGGTTGGCCTATGACGATCAGGGGGAGGGCGTGGCGCTGCTGTGCCT
GCCCGGTCTGACGCGCAACG

Product: haloacid dehalogenase, type II

Products: NA

Alternate protein names: 2-haloalkanoic acid dehalogenase I; DEHCI; Halocarboxylic acid halidohydrolase I; L-2-haloacid dehalogenase I [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTKMTTCVFDAYGTLFDVAAAARELAERPGREAFAEVWQQVANDWRLKQLQYSWLRAVARDHCDFWSVTGDGLDWALERA
GLADMELREELMGLYFTLSAYPEVPEMLGRLKAAGLQTGILSNGSPDMLRGAVESAGLSDLLDAVLSVEDVGVFKPDMRV
YDMIGQRFGCAPGDVLFVSANGWDAAFGAAYGFDTLWVNRGGEPMDRLTGKPGREARDLTGVAALVGA

Sequences:

>Translated_228_residues
MTKMTTCVFDAYGTLFDVAAAARELAERPGREAFAEVWQQVANDWRLKQLQYSWLRAVARDHCDFWSVTGDGLDWALERA
GLADMELREELMGLYFTLSAYPEVPEMLGRLKAAGLQTGILSNGSPDMLRGAVESAGLSDLLDAVLSVEDVGVFKPDMRV
YDMIGQRFGCAPGDVLFVSANGWDAAFGAAYGFDTLWVNRGGEPMDRLTGKPGREARDLTGVAALVGA
>Mature_227_residues
TKMTTCVFDAYGTLFDVAAAARELAERPGREAFAEVWQQVANDWRLKQLQYSWLRAVARDHCDFWSVTGDGLDWALERAG
LADMELREELMGLYFTLSAYPEVPEMLGRLKAAGLQTGILSNGSPDMLRGAVESAGLSDLLDAVLSVEDVGVFKPDMRVY
DMIGQRFGCAPGDVLFVSANGWDAAFGAAYGFDTLWVNRGGEPMDRLTGKPGREARDLTGVAALVGA

Specific function: Catalyzes the hydrolytic dehalogenation of small (S)-2- haloalkanoic acids to yield the corresponding (R)-2- hydroxyalkanoic acids. Acts on acids of short chain lengths, C(2) to C(4), with inversion of configuration at C-2 [H]

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. S-2- haloalkanoic acid dehalogenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006388
- InterPro:   IPR006328
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.8.1.2 [H]

Molecular weight: Translated: 24842; Mature: 24711

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKMTTCVFDAYGTLFDVAAAARELAERPGREAFAEVWQQVANDWRLKQLQYSWLRAVAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHCDFWSVTGDGLDWALERAGLADMELREELMGLYFTLSAYPEVPEMLGRLKAAGLQTGI
HCCCEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
LSNGSPDMLRGAVESAGLSDLLDAVLSVEDVGVFKPDMRVYDMIGQRFGCAPGDVLFVSA
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEC
NGWDAAFGAAYGFDTLWVNRGGEPMDRLTGKPGREARDLTGVAALVGA
CCCCHHHHHHHCCHHEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKMTTCVFDAYGTLFDVAAAARELAERPGREAFAEVWQQVANDWRLKQLQYSWLRAVAR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHCDFWSVTGDGLDWALERAGLADMELREELMGLYFTLSAYPEVPEMLGRLKAAGLQTGI
HCCCEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
LSNGSPDMLRGAVESAGLSDLLDAVLSVEDVGVFKPDMRVYDMIGQRFGCAPGDVLFVSA
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEC
NGWDAAFGAAYGFDTLWVNRGGEPMDRLTGKPGREARDLTGVAALVGA
CCCCHHHHHHHCCHHEEECCCCCHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1995594 [H]