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The map label for this gene is tig

Identifier: 89054104

GI number: 89054104

Start: 1601355

End: 1602680

Strand: Direct

Name: tig

Synonym: Jann_1613

Alternate gene names: 89054104

Gene position: 1601355-1602680 (Clockwise)

Preceding gene: 89054101

Following gene: 89054105

Centisome position: 37.09

GC content: 60.71

Gene sequence:

>1326_bases
ATGCAAATCACCGAGACCCTGGCCGAGGGCCTGAAGCGCGAATACACGATCACCGTCCCCGCGAGTGATCTGGAAGCCCG
CGTGAACACCAAGCTGGAAGAGGCCCGCCCCGAGGTGGAGATGAAGGGCTTCCGCAAGGGCAAGGTGCCCATGGCGCTGC
TGAAAAAGCAGTTCGGCCCGAAGGTCATGGGCGAAGCGATGCAGGAATCGGTCGATGAGGCCATGCAGGGCCATCTGGAC
GAAAGCGGTGATCGCCCCGCTCTCCAGCCCGAGGTCAAGATGACCAACGAGGATTGGAAAGAGGGCGACGATATCGTGGT
CTCCATGGCCTATGAGAAGCTGCCCGAGATCCCCGACGTCGATTACAAGGCGATCAAACTGGAAAAGCTGGTTGTGACGC
CCGGCGACGACGAAGTGAAAGAGGCGCTGGATAATCTGGCCGAAAACGCCGAGAGCTTTGCCACCAAGAAGGGCAAGGCG
GCCGACGGCGACCAGGTCGTGTTCGATTTCGTCGGCACCGTGGACGGTGAGGCGTTTGAAGGCGGATCGGCGGAAGATTT
CCCCCTGAAGCTCGGCTCCGGTCAGTTCATCCCCGGCTTTGAAGAGCAGTTGGTGGGCGTGAAGGCGAAGGACGAGAAAG
ACGTCGAAGTCTCCTTCCCCGAGGATTACCAGGCCGAGCATCTGGCCGGTAAAGCGGCTGTGTTTGCCTGCACCATCAAG
GAAGTGAAGAAGCCCGTCCCGGCCGAGGTTGACGACGAGCTGGCCAAGAAGTTCGGGGCCGAGGATCTGGAGGCGCTGAA
AGGTCAGATCTCCGAGCGTCTGGGCACGGAATACAAAGGCGCCGCGCGGGCGGTGATGAAGCGGTCCCTGCTGGATCAAC
TGGACGATGTCGTGTCGTTTGAGCTGCCGCCCTCGCTGGTGGAGGCCGAGGCCAAGCAGATCGCCCATCAGCTGTGGCAC
GAGGAAAACCCCGAGGTGGAAGGCCACGACCATCCCGAGATCGAGACCACGGAAGAGCACACATCGCTCGCCGCGCGCCG
CGTGAAGCTGGGTCTGTTGCTGGCTGAGCTTGGTCAGAAGAACGATGTGACCGTGTCCGATGCCGAGATGACGCAAGCCA
TCATGACCCAGGCCCGCCAGTACCCGGGTCAGGAGCGGGCGTTCTTTGAGTTCATCCAGCAGAACCAACAGGCCCAACAG
CAGGTCCGCGCGCCGTTGTTTGAAGACAAGGTCGTCGATTTTATCGGTGAGATGGCCGAGGTGTCTGAGAAAGAGGTCTC
CAAAGACGACCTCAAAGCTGCGGTAGATGCCCTCGACGAGGAATAA

Upstream 100 bases:

>100_bases
CATGCCCTCCCCCCATAGAAGGAGCGCGATTTTCAAGACGATTATGCCCGCGCGATCACCTATGACGTCGCCGCGCGCGC
GAGCTGCAAAGAGGTAACCC

Downstream 100 bases:

>100_bases
TCCACGCGACTCGCGCGATGTTTCGCATCCAGGGGGCGTCCTTCACGGGGCGCCCTTTTTTCGTTTGTTAAGCGGCCCTT
AAGCCCTCGTGTTATTGAAT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MQITETLAEGLKREYTITVPASDLEARVNTKLEEARPEVEMKGFRKGKVPMALLKKQFGPKVMGEAMQESVDEAMQGHLD
ESGDRPALQPEVKMTNEDWKEGDDIVVSMAYEKLPEIPDVDYKAIKLEKLVVTPGDDEVKEALDNLAENAESFATKKGKA
ADGDQVVFDFVGTVDGEAFEGGSAEDFPLKLGSGQFIPGFEEQLVGVKAKDEKDVEVSFPEDYQAEHLAGKAAVFACTIK
EVKKPVPAEVDDELAKKFGAEDLEALKGQISERLGTEYKGAARAVMKRSLLDQLDDVVSFELPPSLVEAEAKQIAHQLWH
EENPEVEGHDHPEIETTEEHTSLAARRVKLGLLLAELGQKNDVTVSDAEMTQAIMTQARQYPGQERAFFEFIQQNQQAQQ
QVRAPLFEDKVVDFIGEMAEVSEKEVSKDDLKAAVDALDEE

Sequences:

>Translated_441_residues
MQITETLAEGLKREYTITVPASDLEARVNTKLEEARPEVEMKGFRKGKVPMALLKKQFGPKVMGEAMQESVDEAMQGHLD
ESGDRPALQPEVKMTNEDWKEGDDIVVSMAYEKLPEIPDVDYKAIKLEKLVVTPGDDEVKEALDNLAENAESFATKKGKA
ADGDQVVFDFVGTVDGEAFEGGSAEDFPLKLGSGQFIPGFEEQLVGVKAKDEKDVEVSFPEDYQAEHLAGKAAVFACTIK
EVKKPVPAEVDDELAKKFGAEDLEALKGQISERLGTEYKGAARAVMKRSLLDQLDDVVSFELPPSLVEAEAKQIAHQLWH
EENPEVEGHDHPEIETTEEHTSLAARRVKLGLLLAELGQKNDVTVSDAEMTQAIMTQARQYPGQERAFFEFIQQNQQAQQ
QVRAPLFEDKVVDFIGEMAEVSEKEVSKDDLKAAVDALDEE
>Mature_441_residues
MQITETLAEGLKREYTITVPASDLEARVNTKLEEARPEVEMKGFRKGKVPMALLKKQFGPKVMGEAMQESVDEAMQGHLD
ESGDRPALQPEVKMTNEDWKEGDDIVVSMAYEKLPEIPDVDYKAIKLEKLVVTPGDDEVKEALDNLAENAESFATKKGKA
ADGDQVVFDFVGTVDGEAFEGGSAEDFPLKLGSGQFIPGFEEQLVGVKAKDEKDVEVSFPEDYQAEHLAGKAAVFACTIK
EVKKPVPAEVDDELAKKFGAEDLEALKGQISERLGTEYKGAARAVMKRSLLDQLDDVVSFELPPSLVEAEAKQIAHQLWH
EENPEVEGHDHPEIETTEEHTSLAARRVKLGLLLAELGQKNDVTVSDAEMTQAIMTQARQYPGQERAFFEFIQQNQQAQQ
QVRAPLFEDKVVDFIGEMAEVSEKEVSKDDLKAAVDALDEE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=439, Percent_Identity=31.6628701594533, Blast_Score=205, Evalue=4e-54,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_JANSC (Q28RY2)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509555.1
- ProteinModelPortal:   Q28RY2
- SMR:   Q28RY2
- STRING:   Q28RY2
- GeneID:   3934061
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1613
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q28RY2
- ProtClustDB:   PRK01490
- BioCyc:   JSP290400:JANN_1613-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48795; Mature: 48795

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITETLAEGLKREYTITVPASDLEARVNTKLEEARPEVEMKGFRKGKVPMALLKKQFGP
CCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCH
KVMGEAMQESVDEAMQGHLDESGDRPALQPEVKMTNEDWKEGDDIVVSMAYEKLPEIPDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCC
DYKAIKLEKLVVTPGDDEVKEALDNLAENAESFATKKGKAADGDQVVFDFVGTVDGEAFE
CCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCC
GGSAEDFPLKLGSGQFIPGFEEQLVGVKAKDEKDVEVSFPEDYQAEHLAGKAAVFACTIK
CCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCCCEEECCCCCCHHHHCCCHHHHHHHHH
EVKKPVPAEVDDELAKKFGAEDLEALKGQISERLGTEYKGAARAVMKRSLLDQLDDVVSF
HHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
ELPPSLVEAEAKQIAHQLWHEENPEVEGHDHPEIETTEEHTSLAARRVKLGLLLAELGQK
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
NDVTVSDAEMTQAIMTQARQYPGQERAFFEFIQQNQQAQQQVRAPLFEDKVVDFIGEMAE
CCCEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VSEKEVSKDDLKAAVDALDEE
HHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQITETLAEGLKREYTITVPASDLEARVNTKLEEARPEVEMKGFRKGKVPMALLKKQFGP
CCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCH
KVMGEAMQESVDEAMQGHLDESGDRPALQPEVKMTNEDWKEGDDIVVSMAYEKLPEIPDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCC
DYKAIKLEKLVVTPGDDEVKEALDNLAENAESFATKKGKAADGDQVVFDFVGTVDGEAFE
CCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCC
GGSAEDFPLKLGSGQFIPGFEEQLVGVKAKDEKDVEVSFPEDYQAEHLAGKAAVFACTIK
CCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCCCEEECCCCCCHHHHCCCHHHHHHHHH
EVKKPVPAEVDDELAKKFGAEDLEALKGQISERLGTEYKGAARAVMKRSLLDQLDDVVSF
HHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
ELPPSLVEAEAKQIAHQLWHEENPEVEGHDHPEIETTEEHTSLAARRVKLGLLLAELGQK
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
NDVTVSDAEMTQAIMTQARQYPGQERAFFEFIQQNQQAQQQVRAPLFEDKVVDFIGEMAE
CCCEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VSEKEVSKDDLKAAVDALDEE
HHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA