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The map label for this gene is afr [H]

Identifier: 89053959

GI number: 89053959

Start: 1437784

End: 1438905

Strand: Direct

Name: afr [H]

Synonym: Jann_1468

Alternate gene names: 89053959

Gene position: 1437784-1438905 (Clockwise)

Preceding gene: 89053958

Following gene: 89053960

Centisome position: 33.3

GC content: 63.37

Gene sequence:

>1122_bases
ATGAGCGAGACACGCGACGCCGGTGACAGCGATACTTACGCCTTGAAGGCGGCGGATCTGCCCGAGATTGCCGCGCCCGA
TGTGGCCTACCGCCCGCCGACGCCCAAGATCTATCGCCCGAACATCGGGGTGATTGGAACCGGCGGCATCTCCGGCTCGC
ACCTCGATGCCTACCGTGATGCGGGATGGCAAGTGGCCGCGATGTGGAACCGCACGCCTGCCAAGGCGGAGGCAAAGGCG
GCGGAGTTTTGCCCCAATGCGCGGATCGAGGCGGATTGGCAGGCGATCCTGACCAACCCGGATATCGACGTGGTCGACAT
AACGCTGCACCCCGAACATCGGACCGAGATCATCCGAGCGGCGCTGACAGCGGGCAAGCATGTGCTGAGCCAGAAGCCGT
TTGTCACCGATCTGGATACCGGCATGGACCTGGTGAAACTCGCGCAGGACAGGGGCGTGAAACTGGCCGTGAACCAGAAC
GCACGGTGGGCACCCTATTTTGCATTCATGCGTGAAGCGGCGCGGGCCGGACATATCGGACACGTTCTGTCAGTTCATCT
GGGCGTCCATTGGACCCATGGCTGGACCGCTGGCACGCCCTTTGATGAGATTGAGGATCTGATCCTTTACGACTTCGGTG
TGCACTGGTTCGACTTCGTGAATTCAATCGTGGGGGAGCGGGCCGAGGCCGTCTTTGCCAGCGGTGCGCGCGGGGCGGGG
CAAGCCAACAAGGTGCCGCTGTTTGCGCAGGCCATGGTCAGGATGCAGGGTGGACAAGCCTCCCTGGTGTTTGACGGCGG
TGTGCCCCATGGACCCCGCGACACGACGTTTGTGGGCGGAACGGAAGGGTCCATTGTGTCCGACGGGCCGGACCTCAGCC
AACAGAGTGTCACGCTCACGACGCCCAACGGCATCGCGCGGCCCAAGTTGGACGGTCAATGGTTCAACGACGGCTTCAGG
GGCACGATGGGAGAGCTGTTGTGCGCGATTGAGGAGGATAGAGAACCCTCCAACGACGCGGCCGAAAACCTGCGCAGCCT
GGCGATGGCCTTTGCCGCCGTGCGGTCCCGGATGACGGGGCGCGAAGTTGAGATCGGCGAAGCCCGCCGTTTGGAGAGCT
GA

Upstream 100 bases:

>100_bases
CTGAGGGGCCGTTTGATCCGGCCCTGTGCCTGACCGCGCAACGCATTGTGGACACGGCCGCGGCCTCTGCCGCCGCCAAA
CGCACCCTGGAGATCCTGCC

Downstream 100 bases:

>100_bases
CACTCATGCGCGTTTTGGTCGTCGGATTGGGGAACATGGGCCAAAGCCACGCGCTGGCGCATCACGCCGCCGACGGTGTT
GAAATTATTGGCCTCGTCAA

Product: oxidoreductase-like protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSETRDAGDSDTYALKAADLPEIAAPDVAYRPPTPKIYRPNIGVIGTGGISGSHLDAYRDAGWQVAAMWNRTPAKAEAKA
AEFCPNARIEADWQAILTNPDIDVVDITLHPEHRTEIIRAALTAGKHVLSQKPFVTDLDTGMDLVKLAQDRGVKLAVNQN
ARWAPYFAFMREAARAGHIGHVLSVHLGVHWTHGWTAGTPFDEIEDLILYDFGVHWFDFVNSIVGERAEAVFASGARGAG
QANKVPLFAQAMVRMQGGQASLVFDGGVPHGPRDTTFVGGTEGSIVSDGPDLSQQSVTLTTPNGIARPKLDGQWFNDGFR
GTMGELLCAIEEDREPSNDAAENLRSLAMAFAAVRSRMTGREVEIGEARRLES

Sequences:

>Translated_373_residues
MSETRDAGDSDTYALKAADLPEIAAPDVAYRPPTPKIYRPNIGVIGTGGISGSHLDAYRDAGWQVAAMWNRTPAKAEAKA
AEFCPNARIEADWQAILTNPDIDVVDITLHPEHRTEIIRAALTAGKHVLSQKPFVTDLDTGMDLVKLAQDRGVKLAVNQN
ARWAPYFAFMREAARAGHIGHVLSVHLGVHWTHGWTAGTPFDEIEDLILYDFGVHWFDFVNSIVGERAEAVFASGARGAG
QANKVPLFAQAMVRMQGGQASLVFDGGVPHGPRDTTFVGGTEGSIVSDGPDLSQQSVTLTTPNGIARPKLDGQWFNDGFR
GTMGELLCAIEEDREPSNDAAENLRSLAMAFAAVRSRMTGREVEIGEARRLES
>Mature_372_residues
SETRDAGDSDTYALKAADLPEIAAPDVAYRPPTPKIYRPNIGVIGTGGISGSHLDAYRDAGWQVAAMWNRTPAKAEAKAA
EFCPNARIEADWQAILTNPDIDVVDITLHPEHRTEIIRAALTAGKHVLSQKPFVTDLDTGMDLVKLAQDRGVKLAVNQNA
RWAPYFAFMREAARAGHIGHVLSVHLGVHWTHGWTAGTPFDEIEDLILYDFGVHWFDFVNSIVGERAEAVFASGARGAGQ
ANKVPLFAQAMVRMQGGQASLVFDGGVPHGPRDTTFVGGTEGSIVSDGPDLSQQSVTLTTPNGIARPKLDGQWFNDGFRG
TMGELLCAIEEDREPSNDAAENLRSLAMAFAAVRSRMTGREVEIGEARRLES

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI1789848, Length=191, Percent_Identity=29.3193717277487, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323975, Length=313, Percent_Identity=26.1980830670927, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 40372; Mature: 40240

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETRDAGDSDTYALKAADLPEIAAPDVAYRPPTPKIYRPNIGVIGTGGISGSHLDAYRD
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCCCHHHHHC
AGWQVAAMWNRTPAKAEAKAAEFCPNARIEADWQAILTNPDIDVVDITLHPEHRTEIIRA
CCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHH
ALTAGKHVLSQKPFVTDLDTGMDLVKLAQDRGVKLAVNQNARWAPYFAFMREAARAGHIG
HHHHHHHHHCCCCCCEECCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
HVLSVHLGVHWTHGWTAGTPFDEIEDLILYDFGVHWFDFVNSIVGERAEAVFASGARGAG
EEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC
QANKVPLFAQAMVRMQGGQASLVFDGGVPHGPRDTTFVGGTEGSIVSDGPDLSQQSVTLT
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCEECCCCCCCCCEEEEE
TPNGIARPKLDGQWFNDGFRGTMGELLCAIEEDREPSNDAAENLRSLAMAFAAVRSRMTG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
REVEIGEARRLES
CCEECCCHHCCCC
>Mature Secondary Structure 
SETRDAGDSDTYALKAADLPEIAAPDVAYRPPTPKIYRPNIGVIGTGGISGSHLDAYRD
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCCCHHHHHC
AGWQVAAMWNRTPAKAEAKAAEFCPNARIEADWQAILTNPDIDVVDITLHPEHRTEIIRA
CCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECCHHHHHHHHH
ALTAGKHVLSQKPFVTDLDTGMDLVKLAQDRGVKLAVNQNARWAPYFAFMREAARAGHIG
HHHHHHHHHCCCCCCEECCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCC
HVLSVHLGVHWTHGWTAGTPFDEIEDLILYDFGVHWFDFVNSIVGERAEAVFASGARGAG
EEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC
QANKVPLFAQAMVRMQGGQASLVFDGGVPHGPRDTTFVGGTEGSIVSDGPDLSQQSVTLT
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCCCCEECCCCCCCCCEEEEE
TPNGIARPKLDGQWFNDGFRGTMGELLCAIEEDREPSNDAAENLRSLAMAFAAVRSRMTG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
REVEIGEARRLES
CCEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA