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The map label for this gene is ndhF [H]

Identifier: 89053750

GI number: 89053750

Start: 1217313

End: 1218872

Strand: Reverse

Name: ndhF [H]

Synonym: Jann_1259

Alternate gene names: 89053750

Gene position: 1218872-1217313 (Counterclockwise)

Preceding gene: 89053755

Following gene: 89053749

Centisome position: 28.23

GC content: 65.64

Gene sequence:

>1560_bases
TTGTTTATCGCCTTCCTGCCCCTTGTGGCCCCGGTCGTCTTTGTCGGTCTCGCGGCCATCGCGTTTCGTGGACCGCAGAT
GCGCCCGACAGCGGTGTTAAGACAGGTCGAAATCGGCGCGCTGGGTGTCCTGGCCCTGGCTGCGTTGGCGGCTTTGATGC
TTATCCTGACAGGCCCGGGGACCAGCCCTCTGATCGGTGCGAACGGTCTTGGACTATCCGCGCGGCTCGATGCGATCAGC
GGTGTTATGCTGTTGCTGGTCGCGTTCGTGGGCTGGGTCGTGGTGCGCTATTCCGCAACATATATGGACGGCGAAGCGCG
GCAGGGGCCCTTTACCGGCTGGCTATGCCTCACGCTCGCGGCTGTGATGCTGCTGGTGATATCCGGCAACCTTCTGCAAT
TTGCCCTTGCGTGGATCGCCACCAGTCTGTCGCTGCACAAGCTGCTGCTGCATTACCCTGAGCGGGTTGCAGCCCGGCGG
GCGGCGCGCAAGAAATGGATCACCGCCCGGCTGGGGGACGCCGCGATGCTGGCGGCGATGATCCTGATCTTCATCACCTA
TGAAACGGGTGATCTGGCAACCATTCTGGACGCAGCGCGCAGCGGTCAGGGCAGCGAGCTGGCCACGTGGATCGCCGCCG
CCCTCGCCCTCGCGGCCATCCTGAAATCCGCGCAATTCCCGATGCACGGCTGGCTGACCGAGGTGATGGAGACGCCAACG
CCGGTATCCGCGCTGCTGCACGCAGGCGTCATCAATGCGGGCGGCTTCCTGTTGATCCGCTTTGCCGACGTGATGCTGCT
GTCGCCGGGCGTGCTGGCGGTGCTGGTGATGATCGGCGGGTTCACGGCGCTTTTCGGCGGTCTGGTGATGCTGACGCAAT
CGGCGGTCAAAACCTCCCTCGCGTGGTCCACGGTGGCGCAGATGGGGTTCATGATCCTGCAATGCGGTCTGGCGCTGTTC
CCGCTGGCGCTGCTGCATATCGTGGCGCATTCGATGTATAAGGCGCATGCCTTTCTTGCCTCTGGCGGTGCCGTGGACGG
CGTCGCCGCGATCCGCAGGCCGGGCCCCGTCGCGATCCCCAACGGCGGGGCGGTGGGGCGCGCGTTTCTGATCGCGCTGG
TCATCTACGGCGCGGTCGGGCTGATCTTCGGCCTGACGGGCAAGTCGCCGCAAGCCATCGCCCTGGGCGCGATCCTGATC
TTCGGCGTGGCGTATCTTTTGGCGCAAGGCCTGGCCGACGCAGCCCCGCGTGTTCTGACGCAACGCACCGCAGCCTATTC
CGTCGCAGCGGCGGTGGGTTACTTTGCCCTGCAAACCGCAGCCGAGTGGCTGATGGCAGGCACCCTTCCCGCCACGCCCG
CCCCCGGCCCGCTGGAATGGGCGTTGATCGTGCTGGCCGTGCTCAGCTTTGGGGTGGTGGCGGTGGCGCAGGCGATGTTC
CCGCTCTGGGCCTATCACCCCGCCGCGGCCGGATTGCGGGTGCATCTGTCCAACGGGCTGTATGCCAATGCCGTCTTCGA
CAAGCTTCTGGGTGGCTGGACCACCGAAAACCGCTCCTGA

Upstream 100 bases:

>100_bases
ATATATTAAACAGAATGATTTCCGCTAAACAATGAATTTTTCTAACGTAGATGAGGCTGCTATCTGAACCGGACACGGTC
TGTCAGAAGGATGCCCTGCT

Downstream 100 bases:

>100_bases
TTTCGGGGAAGGATAGATCCGATGACCACCGTCCATGACCTCTCGTCCGACACCATGACCACAGCCGCCGATCGCGCCGC
ACGCGCCATTCCTCCGGTTT

Product: NADH dehydrogenase (quinone)

Products: NAD+; ubiquinol [C]

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MFIAFLPLVAPVVFVGLAAIAFRGPQMRPTAVLRQVEIGALGVLALAALAALMLILTGPGTSPLIGANGLGLSARLDAIS
GVMLLLVAFVGWVVVRYSATYMDGEARQGPFTGWLCLTLAAVMLLVISGNLLQFALAWIATSLSLHKLLLHYPERVAARR
AARKKWITARLGDAAMLAAMILIFITYETGDLATILDAARSGQGSELATWIAAALALAAILKSAQFPMHGWLTEVMETPT
PVSALLHAGVINAGGFLLIRFADVMLLSPGVLAVLVMIGGFTALFGGLVMLTQSAVKTSLAWSTVAQMGFMILQCGLALF
PLALLHIVAHSMYKAHAFLASGGAVDGVAAIRRPGPVAIPNGGAVGRAFLIALVIYGAVGLIFGLTGKSPQAIALGAILI
FGVAYLLAQGLADAAPRVLTQRTAAYSVAAAVGYFALQTAAEWLMAGTLPATPAPGPLEWALIVLAVLSFGVVAVAQAMF
PLWAYHPAAAGLRVHLSNGLYANAVFDKLLGGWTTENRS

Sequences:

>Translated_519_residues
MFIAFLPLVAPVVFVGLAAIAFRGPQMRPTAVLRQVEIGALGVLALAALAALMLILTGPGTSPLIGANGLGLSARLDAIS
GVMLLLVAFVGWVVVRYSATYMDGEARQGPFTGWLCLTLAAVMLLVISGNLLQFALAWIATSLSLHKLLLHYPERVAARR
AARKKWITARLGDAAMLAAMILIFITYETGDLATILDAARSGQGSELATWIAAALALAAILKSAQFPMHGWLTEVMETPT
PVSALLHAGVINAGGFLLIRFADVMLLSPGVLAVLVMIGGFTALFGGLVMLTQSAVKTSLAWSTVAQMGFMILQCGLALF
PLALLHIVAHSMYKAHAFLASGGAVDGVAAIRRPGPVAIPNGGAVGRAFLIALVIYGAVGLIFGLTGKSPQAIALGAILI
FGVAYLLAQGLADAAPRVLTQRTAAYSVAAAVGYFALQTAAEWLMAGTLPATPAPGPLEWALIVLAVLSFGVVAVAQAMF
PLWAYHPAAAGLRVHLSNGLYANAVFDKLLGGWTTENRS
>Mature_519_residues
MFIAFLPLVAPVVFVGLAAIAFRGPQMRPTAVLRQVEIGALGVLALAALAALMLILTGPGTSPLIGANGLGLSARLDAIS
GVMLLLVAFVGWVVVRYSATYMDGEARQGPFTGWLCLTLAAVMLLVISGNLLQFALAWIATSLSLHKLLLHYPERVAARR
AARKKWITARLGDAAMLAAMILIFITYETGDLATILDAARSGQGSELATWIAAALALAAILKSAQFPMHGWLTEVMETPT
PVSALLHAGVINAGGFLLIRFADVMLLSPGVLAVLVMIGGFTALFGGLVMLTQSAVKTSLAWSTVAQMGFMILQCGLALF
PLALLHIVAHSMYKAHAFLASGGAVDGVAAIRRPGPVAIPNGGAVGRAFLIALVIYGAVGLIFGLTGKSPQAIALGAILI
FGVAYLLAQGLADAAPRVLTQRTAAYSVAAAVGYFALQTAAEWLMAGTLPATPAPGPLEWALIVLAVLSFGVVAVAQAMF
PLWAYHPAAAGLRVHLSNGLYANAVFDKLLGGWTTENRS

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=293, Percent_Identity=33.1058020477816, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1788614, Length=284, Percent_Identity=32.3943661971831, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788829, Length=284, Percent_Identity=30.6338028169014, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788831, Length=307, Percent_Identity=28.6644951140065, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788827, Length=304, Percent_Identity=27.6315789473684, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 53920; Mature: 53920

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIAFLPLVAPVVFVGLAAIAFRGPQMRPTAVLRQVEIGALGVLALAALAALMLILTGPG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TSPLIGANGLGLSARLDAISGVMLLLVAFVGWVVVRYSATYMDGEARQGPFTGWLCLTLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHH
AVMLLVISGNLLQFALAWIATSLSLHKLLLHYPERVAARRAARKKWITARLGDAAMLAAM
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ILIFITYETGDLATILDAARSGQGSELATWIAAALALAAILKSAQFPMHGWLTEVMETPT
HHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
PVSALLHAGVINAGGFLLIRFADVMLLSPGVLAVLVMIGGFTALFGGLVMLTQSAVKTSL
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWSTVAQMGFMILQCGLALFPLALLHIVAHSMYKAHAFLASGGAVDGVAAIRRPGPVAIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEECC
NGGAVGRAFLIALVIYGAVGLIFGLTGKSPQAIALGAILIFGVAYLLAQGLADAAPRVLT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRTAAYSVAAAVGYFALQTAAEWLMAGTLPATPAPGPLEWALIVLAVLSFGVVAVAQAMF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PLWAYHPAAAGLRVHLSNGLYANAVFDKLLGGWTTENRS
HHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFIAFLPLVAPVVFVGLAAIAFRGPQMRPTAVLRQVEIGALGVLALAALAALMLILTGPG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TSPLIGANGLGLSARLDAISGVMLLLVAFVGWVVVRYSATYMDGEARQGPFTGWLCLTLA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHH
AVMLLVISGNLLQFALAWIATSLSLHKLLLHYPERVAARRAARKKWITARLGDAAMLAAM
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ILIFITYETGDLATILDAARSGQGSELATWIAAALALAAILKSAQFPMHGWLTEVMETPT
HHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
PVSALLHAGVINAGGFLLIRFADVMLLSPGVLAVLVMIGGFTALFGGLVMLTQSAVKTSL
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWSTVAQMGFMILQCGLALFPLALLHIVAHSMYKAHAFLASGGAVDGVAAIRRPGPVAIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEECC
NGGAVGRAFLIALVIYGAVGLIFGLTGKSPQAIALGAILIFGVAYLLAQGLADAAPRVLT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRTAAYSVAAAVGYFALQTAAEWLMAGTLPATPAPGPLEWALIVLAVLSFGVVAVAQAMF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PLWAYHPAAAGLRVHLSNGLYANAVFDKLLGGWTTENRS
HHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]