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The map label for this gene is gatA

Identifier: 89053712

GI number: 89053712

Start: 1179939

End: 1181423

Strand: Reverse

Name: gatA

Synonym: Jann_1221

Alternate gene names: 89053712

Gene position: 1181423-1179939 (Counterclockwise)

Preceding gene: 89053713

Following gene: 89053711

Centisome position: 27.36

GC content: 63.97

Gene sequence:

>1485_bases
ATGTCTGATTTGAATGCCTTGACCATTGCCGACGCCCGGGACGCCCTGCGCAAGGGAGAGGTCACATCCGTGGACCTGAC
CCAGGCCTGCCTGTCCGCGATTGACCAAGCCGATGCGCTTGGCACCTTCGTCCACAAGACGCCGGATCTGGCGCTGGAAC
GTGCGAAGGCCGCGGACGCCCGGATCGCAGAGGGTGACGCGCCCGACATGTGCGGCATTCCCGTGGGCATCAAGGATCTG
TTTTGCACCGAAGGTGTCCCCAGCCAGGCGGCATCCAGAATCCTGGACGGCTTCAAACCGGAATACGAATCCACCGTGTC
TGGCAAGTTGCGGGACGCGGGCGCGGTGATGTTGGGCAAGCTGAACATGGACGAATTCGCCATGGGCTCCTCCAACGAAA
CGTCCGTTTACGGCAATGCCGTGAACCCATGGCGCGCGGGCAATTCCGACGCGGCCCTGACACCGGGGGGATCGTCAGGC
GGGAGTGCGTCAGCTGTGGCGGGCGATCTGTGTTTGGCGGCAACGGGTACGGATACGGGCGGCTCCATCCGGCAACCGGC
GGCGTTCACCGGCATCGTTGGCCTGAAGCCCACCTACGGGCGGTGCTCGCGCTGGGGGATCGTGGCGTTTGCGTCATCCC
TCGATCAGGCCGGGCCGATGACCAAAACCGTGCGCGACGCGGCGATCATGGGTCGTGCGATGATGGGCTATGACGCGAAG
GACAGCACGTCTGCCAATATCGACGTGCCGGATTTTGAGGCGATGCTGACCGGCGACATCCGCGGCAAAATCATTGGTAT
TCCAAAGGAATATCGCATGGACGGCATGCCAGACGAGATTGAGACACTCTGGGCCGACGGCACCGCAATGCTGAAGGACG
CGGGCGCTGTGATCCGCGATATCTCCCTGCCGCACACGAAATACGCCCTGCCCGCCTATTATGTGATCGCACCGGCGGAG
GCGTCATCGAACCTCGCGCGCTATGATGGCGTGCGCTTCGGCTACCGCGCCGATATGGCGCCGGGTGACGGCATCACCGA
GATGTATGAAAAGACCCGCGCGGAAGGCTTCGGGCCCGAGGTGCAGCGCCGCGTGATGATCGGCACCTATGTGCTCTCCG
CCGGGTTCTATGACGCCTATTACAACCGGGCGCGGCGCGTGCGGGCGTTGATCAAGCGTGATTTCGATGAGGTGTTCGCC
GCCGGTGTCGATGCAATCCTGACCCCCGCCACCCCCTCTGCCGCGTTCGAGTTGGGCAAGGAGGTCAGCGACCCGGTCGA
GATGTATCTGAACGACGTCTTCACCGTGACGGTCAACCTCGCGGGTCTTCCGGGCGTCGCGGTGCCGACGGGCGTGGACC
GCAACGGTCTGCCCCTTGGGTTGCAACTGATCGGCAAGCCGTGGGAGGAGGGCGAGTTGCTGAACACCGCCTATGCGCTG
GAGGCGGCCGCCGGTTTTGTGGCAAAGCCCGCGCGTTGGTGGTAG

Upstream 100 bases:

>100_bases
GCGAAGATGTGGTGACAGACGGTGACCAACAGACCCGCGTTCTGACCAATGCACCTGATGCGCGTGAAGGCTTCTTCTCA
GTCCCGAAAGTGGTGGAATA

Downstream 100 bases:

>100_bases
AGACCTCCTCAACCAAACGACATGAGGCTTCAGTGATGGGTTCCCCGCGATATCTTGTGCTGGCGTGCATGGCCCTTGCG
GCTTGCGCCAGCCCGCCCAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSDLNALTIADARDALRKGEVTSVDLTQACLSAIDQADALGTFVHKTPDLALERAKAADARIAEGDAPDMCGIPVGIKDL
FCTEGVPSQAASRILDGFKPEYESTVSGKLRDAGAVMLGKLNMDEFAMGSSNETSVYGNAVNPWRAGNSDAALTPGGSSG
GSASAVAGDLCLAATGTDTGGSIRQPAAFTGIVGLKPTYGRCSRWGIVAFASSLDQAGPMTKTVRDAAIMGRAMMGYDAK
DSTSANIDVPDFEAMLTGDIRGKIIGIPKEYRMDGMPDEIETLWADGTAMLKDAGAVIRDISLPHTKYALPAYYVIAPAE
ASSNLARYDGVRFGYRADMAPGDGITEMYEKTRAEGFGPEVQRRVMIGTYVLSAGFYDAYYNRARRVRALIKRDFDEVFA
AGVDAILTPATPSAAFELGKEVSDPVEMYLNDVFTVTVNLAGLPGVAVPTGVDRNGLPLGLQLIGKPWEEGELLNTAYAL
EAAAGFVAKPARWW

Sequences:

>Translated_494_residues
MSDLNALTIADARDALRKGEVTSVDLTQACLSAIDQADALGTFVHKTPDLALERAKAADARIAEGDAPDMCGIPVGIKDL
FCTEGVPSQAASRILDGFKPEYESTVSGKLRDAGAVMLGKLNMDEFAMGSSNETSVYGNAVNPWRAGNSDAALTPGGSSG
GSASAVAGDLCLAATGTDTGGSIRQPAAFTGIVGLKPTYGRCSRWGIVAFASSLDQAGPMTKTVRDAAIMGRAMMGYDAK
DSTSANIDVPDFEAMLTGDIRGKIIGIPKEYRMDGMPDEIETLWADGTAMLKDAGAVIRDISLPHTKYALPAYYVIAPAE
ASSNLARYDGVRFGYRADMAPGDGITEMYEKTRAEGFGPEVQRRVMIGTYVLSAGFYDAYYNRARRVRALIKRDFDEVFA
AGVDAILTPATPSAAFELGKEVSDPVEMYLNDVFTVTVNLAGLPGVAVPTGVDRNGLPLGLQLIGKPWEEGELLNTAYAL
EAAAGFVAKPARWW
>Mature_493_residues
SDLNALTIADARDALRKGEVTSVDLTQACLSAIDQADALGTFVHKTPDLALERAKAADARIAEGDAPDMCGIPVGIKDLF
CTEGVPSQAASRILDGFKPEYESTVSGKLRDAGAVMLGKLNMDEFAMGSSNETSVYGNAVNPWRAGNSDAALTPGGSSGG
SASAVAGDLCLAATGTDTGGSIRQPAAFTGIVGLKPTYGRCSRWGIVAFASSLDQAGPMTKTVRDAAIMGRAMMGYDAKD
STSANIDVPDFEAMLTGDIRGKIIGIPKEYRMDGMPDEIETLWADGTAMLKDAGAVIRDISLPHTKYALPAYYVIAPAEA
SSNLARYDGVRFGYRADMAPGDGITEMYEKTRAEGFGPEVQRRVMIGTYVLSAGFYDAYYNRARRVRALIKRDFDEVFAA
GVDAILTPATPSAAFELGKEVSDPVEMYLNDVFTVTVNLAGLPGVAVPTGVDRNGLPLGLQLIGKPWEEGELLNTAYALE
AAAGFVAKPARWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=39.5209580838323, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI195972892, Length=506, Percent_Identity=23.9130434782609, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI166795287, Length=153, Percent_Identity=32.6797385620915, Blast_Score=66, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=457, Percent_Identity=35.2297592997812, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17537465, Length=504, Percent_Identity=25.3968253968254, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17538252, Length=242, Percent_Identity=25.6198347107438, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=208, Percent_Identity=28.8461538461538, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=220, Percent_Identity=27.7272727272727, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=235, Percent_Identity=28.0851063829787, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=430, Percent_Identity=34.8837209302326, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=413, Percent_Identity=26.634382566586, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320448, Length=205, Percent_Identity=28.780487804878, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648113, Length=482, Percent_Identity=37.9668049792531, Blast_Score=295, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24652985, Length=496, Percent_Identity=24.1935483870968, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19922090, Length=496, Percent_Identity=24.1935483870968, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24652981, Length=496, Percent_Identity=24.1935483870968, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24652983, Length=496, Percent_Identity=24.1935483870968, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI161078093, Length=513, Percent_Identity=23.9766081871345, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24644968, Length=246, Percent_Identity=28.8617886178862, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356731, Length=505, Percent_Identity=24.3564356435644, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550774, Length=253, Percent_Identity=26.8774703557312, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648435, Length=253, Percent_Identity=26.8774703557312, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648437, Length=253, Percent_Identity=26.8774703557312, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=28.9772727272727, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=28.9772727272727, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_JANSC (Q28T24)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509163.1
- ProteinModelPortal:   Q28T24
- SMR:   Q28T24
- STRING:   Q28T24
- GeneID:   3933666
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1221
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- PhylomeDB:   Q28T24
- ProtClustDB:   PRK00012
- BioCyc:   JSP290400:JANN_1221-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52225; Mature: 52093

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 158-158 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLNALTIADARDALRKGEVTSVDLTQACLSAIDQADALGTFVHKTPDLALERAKAADA
CCCCCEEEEHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
RIAEGDAPDMCGIPVGIKDLFCTEGVPSQAASRILDGFKPEYESTVSGKLRDAGAVMLGK
EECCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEEE
LNMDEFAMGSSNETSVYGNAVNPWRAGNSDAALTPGGSSGGSASAVAGDLCLAATGTDTG
CCHHHHCCCCCCCCEEECCCCCCCCCCCCCCEECCCCCCCCCCHHHHCCEEEEECCCCCC
GSIRQPAAFTGIVGLKPTYGRCSRWGIVAFASSLDQAGPMTKTVRDAAIMGRAMMGYDAK
CCCCCCCHHEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
DSTSANIDVPDFEAMLTGDIRGKIIGIPKEYRMDGMPDEIETLWADGTAMLKDAGAVIRD
CCCCCCCCCCCCHHEEECCCCCEEECCCHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
ISLPHTKYALPAYYVIAPAEASSNLARYDGVRFGYRADMAPGDGITEMYEKTRAEGFGPE
CCCCCCCCCCCEEEEEEECCCCCCHHHHCCCEECEECCCCCCCCHHHHHHHHHHCCCCHH
VQRRVMIGTYVLSAGFYDAYYNRARRVRALIKRDFDEVFAAGVDAILTPATPSAAFELGK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHH
EVSDPVEMYLNDVFTVTVNLAGLPGVAVPTGVDRNGLPLGLQLIGKPWEEGELLNTAYAL
HCCHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCCCCCCHHHHHHHH
EAAAGFVAKPARWW
HHHHHHHCCCCCCC
>Mature Secondary Structure 
SDLNALTIADARDALRKGEVTSVDLTQACLSAIDQADALGTFVHKTPDLALERAKAADA
CCCCEEEEHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
RIAEGDAPDMCGIPVGIKDLFCTEGVPSQAASRILDGFKPEYESTVSGKLRDAGAVMLGK
EECCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEEE
LNMDEFAMGSSNETSVYGNAVNPWRAGNSDAALTPGGSSGGSASAVAGDLCLAATGTDTG
CCHHHHCCCCCCCCEEECCCCCCCCCCCCCCEECCCCCCCCCCHHHHCCEEEEECCCCCC
GSIRQPAAFTGIVGLKPTYGRCSRWGIVAFASSLDQAGPMTKTVRDAAIMGRAMMGYDAK
CCCCCCCHHEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
DSTSANIDVPDFEAMLTGDIRGKIIGIPKEYRMDGMPDEIETLWADGTAMLKDAGAVIRD
CCCCCCCCCCCCHHEEECCCCCEEECCCHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
ISLPHTKYALPAYYVIAPAEASSNLARYDGVRFGYRADMAPGDGITEMYEKTRAEGFGPE
CCCCCCCCCCCEEEEEEECCCCCCHHHHCCCEECEECCCCCCCCHHHHHHHHHHCCCCHH
VQRRVMIGTYVLSAGFYDAYYNRARRVRALIKRDFDEVFAAGVDAILTPATPSAAFELGK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHH
EVSDPVEMYLNDVFTVTVNLAGLPGVAVPTGVDRNGLPLGLQLIGKPWEEGELLNTAYAL
HCCHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCCCCCCHHHHHHHH
EAAAGFVAKPARWW
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA