Click here to switch to the map view.

The map label for this gene is prfC [H]

Identifier: 89053694

GI number: 89053694

Start: 1164035

End: 1165672

Strand: Reverse

Name: prfC [H]

Synonym: Jann_1203

Alternate gene names: 89053694

Gene position: 1165672-1164035 (Counterclockwise)

Preceding gene: 89053695

Following gene: 89053693

Centisome position: 27.0

GC content: 61.78

Gene sequence:

>1638_bases
ATGCGGGCCATGCCCGATACAGCACACACCAATCGCCCCACCCTGCCGCCCGAGATCGCGCGTCGTCGCACATTCGCGAT
CATCTCGCACCCCGATGCGGGAAAAACGACGCTGACCGAGAAGTTTCTTCTGTATGGTGGCGCGATCCAGATGGCCGGAC
AGGTGCGCGCCAAAGGTGAGGCGCGGCGCACGCGCTCGGACTTCATGCAGATGGAAAAGGACCGGGGCATTTCCGTCTCG
GCCTCCGCCATGTCGTTTGACTTCCTGACACCGGATGTGAATTTTCGCTTCAATCTGGTCGACACGCCCGGCCACAGCGA
CTTCTCGGAAGACACCTACCGCACGCTGACCGCCGTGGACGCCGCGATCATGGTAATCGACGGCGCGAAGGGTGTGGAAA
GCCAGACCCAGAAGCTGTTCGAGGTCTGCCGCCTGCGCGATCTGCCGATCCTGACATTCTGCAACAAGATGGACCGTGAA
AGCCGCGACACGTTCGAGATCATCGACGAAATTCAGGAAAATCTGGCGATTGACGTCACACCCGCCTCATGGCCCATCGG
CGTCGGGCGGGAATTTCTGGGCTGCTACGACCTGCTCCACGACCGGCTGGAGCTGATGGACCGCGCCGACCGCAACAAGG
TGGCAGAAACGGTGTCTTTGCAGGGCCTCGACGATCCGGCCCTGGCTGATCACATCCCCGCTGACCTGTTGGACAAGCTG
CGTGAAGAGGTCGAAATGGCCCGCGAATTGCTGCCCGCCATGGATCCTGAGGCGTTTCAACAGGGCACGCTGACTCCGAT
CTGGTTCGGCTCGGCCATGAACTCCTTCGGCGTGAAGGAGCTGATGGACGGCATCGCGGAATACGGGCCTGAGCCGCAGG
TACAAAACGCCGATCCTCGGCAAGTGGCCCCCGATGAGAAGAAGGTTTCGGGTTTTGTGTTCAAGGTGCAGGCCAATATG
GACCCCAAGCACCGGGACCGGGTGGCGTTTGTGCGGTTGGTCTCGGGCCATTTCAATCGTGGCATGAAGCTTACGCACGT
GCGGTCGAAAAAGCCCATGGCGATCAGCAATCCGGTGATGTTTCTGGCCTCTGACCGGGAATTGGCGGAAGAAGCCTGGG
CCGGCGACATCATCGGCATCCCCAATCACGGCCAATTGCGCATCGGCGACGCGCTGACGGAAGGGGAGGCGCTGCGCTTC
ACCGGCATCCCGTCCTTCGCGCCGGAACTGCTTCAGTCCTGCCGCGCGGGCGATCCGCTGAAGGCCAAACATCTCGATAA
GGCCTTGATGCAATTCGCAGAAGAGGGGGCGGCCAAGGTGTTCAAACCGACCTTCGGGTCCGGCTTTATCGTCGGCGTCG
TGGGCGCGCTTCAATTTGAAGTGCTCGCGAGCCGGATCGAGCTGGAATACGGTCTTCCCGTCCGGTTCGAGGCGTCTCAA
TTCACCTCCGCCCGCTGGGTGCACGGCGACAAGGACGCGGTGGAGGCCTTCGCCAACGCCAACAAGCAGCACATCGCCAC
GGACAACGACGGCGACCCGGTCTACCTCACGCGCCTGCAATGGGACATCGACCGCGTGGCCCGCGATTATCCCGACATCA
CGCTGTCGGCGACGAAAGAACTGATGGTCGAGCGCTGA

Upstream 100 bases:

>100_bases
CGTTGGTCGCTAACCCGTTTGGGCGGCAGGAGGACGCTTCTGCCACCCATCAAACCACGACCTGATTTCCGCGATTGTCC
CTTGGGCCTGCGACCAATAT

Downstream 100 bases:

>100_bases
TGCCCCTGCGCAACCGCGTCCTGCCCACCGGTGAGATTGTCGCCGATCCGGCGCGCGGCACGTTGACGGGCAACCGCGGA
ATCCTCGTGGCCTCTGACGG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MRAMPDTAHTNRPTLPPEIARRRTFAIISHPDAGKTTLTEKFLLYGGAIQMAGQVRAKGEARRTRSDFMQMEKDRGISVS
ASAMSFDFLTPDVNFRFNLVDTPGHSDFSEDTYRTLTAVDAAIMVIDGAKGVESQTQKLFEVCRLRDLPILTFCNKMDRE
SRDTFEIIDEIQENLAIDVTPASWPIGVGREFLGCYDLLHDRLELMDRADRNKVAETVSLQGLDDPALADHIPADLLDKL
REEVEMARELLPAMDPEAFQQGTLTPIWFGSAMNSFGVKELMDGIAEYGPEPQVQNADPRQVAPDEKKVSGFVFKVQANM
DPKHRDRVAFVRLVSGHFNRGMKLTHVRSKKPMAISNPVMFLASDRELAEEAWAGDIIGIPNHGQLRIGDALTEGEALRF
TGIPSFAPELLQSCRAGDPLKAKHLDKALMQFAEEGAAKVFKPTFGSGFIVGVVGALQFEVLASRIELEYGLPVRFEASQ
FTSARWVHGDKDAVEAFANANKQHIATDNDGDPVYLTRLQWDIDRVARDYPDITLSATKELMVER

Sequences:

>Translated_545_residues
MRAMPDTAHTNRPTLPPEIARRRTFAIISHPDAGKTTLTEKFLLYGGAIQMAGQVRAKGEARRTRSDFMQMEKDRGISVS
ASAMSFDFLTPDVNFRFNLVDTPGHSDFSEDTYRTLTAVDAAIMVIDGAKGVESQTQKLFEVCRLRDLPILTFCNKMDRE
SRDTFEIIDEIQENLAIDVTPASWPIGVGREFLGCYDLLHDRLELMDRADRNKVAETVSLQGLDDPALADHIPADLLDKL
REEVEMARELLPAMDPEAFQQGTLTPIWFGSAMNSFGVKELMDGIAEYGPEPQVQNADPRQVAPDEKKVSGFVFKVQANM
DPKHRDRVAFVRLVSGHFNRGMKLTHVRSKKPMAISNPVMFLASDRELAEEAWAGDIIGIPNHGQLRIGDALTEGEALRF
TGIPSFAPELLQSCRAGDPLKAKHLDKALMQFAEEGAAKVFKPTFGSGFIVGVVGALQFEVLASRIELEYGLPVRFEASQ
FTSARWVHGDKDAVEAFANANKQHIATDNDGDPVYLTRLQWDIDRVARDYPDITLSATKELMVER
>Mature_545_residues
MRAMPDTAHTNRPTLPPEIARRRTFAIISHPDAGKTTLTEKFLLYGGAIQMAGQVRAKGEARRTRSDFMQMEKDRGISVS
ASAMSFDFLTPDVNFRFNLVDTPGHSDFSEDTYRTLTAVDAAIMVIDGAKGVESQTQKLFEVCRLRDLPILTFCNKMDRE
SRDTFEIIDEIQENLAIDVTPASWPIGVGREFLGCYDLLHDRLELMDRADRNKVAETVSLQGLDDPALADHIPADLLDKL
REEVEMARELLPAMDPEAFQQGTLTPIWFGSAMNSFGVKELMDGIAEYGPEPQVQNADPRQVAPDEKKVSGFVFKVQANM
DPKHRDRVAFVRLVSGHFNRGMKLTHVRSKKPMAISNPVMFLASDRELAEEAWAGDIIGIPNHGQLRIGDALTEGEALRF
TGIPSFAPELLQSCRAGDPLKAKHLDKALMQFAEEGAAKVFKPTFGSGFIVGVVGALQFEVLASRIELEYGLPVRFEASQ
FTSARWVHGDKDAVEAFANANKQHIATDNDGDPVYLTRLQWDIDRVARDYPDITLSATKELMVER

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=483, Percent_Identity=26.7080745341615, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI19923640, Length=506, Percent_Identity=28.0632411067194, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI25306283, Length=382, Percent_Identity=29.5811518324607, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI25306287, Length=481, Percent_Identity=28.0665280665281, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI157426893, Length=151, Percent_Identity=31.1258278145695, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI94966754, Length=142, Percent_Identity=33.0985915492958, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI4503483, Length=153, Percent_Identity=35.2941176470588, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310132016, Length=123, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI310110807, Length=123, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI310123363, Length=123, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1790835, Length=531, Percent_Identity=48.210922787194, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1789738, Length=486, Percent_Identity=26.5432098765432, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1788922, Length=174, Percent_Identity=33.9080459770115, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI48994988, Length=153, Percent_Identity=31.3725490196078, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17533571, Length=499, Percent_Identity=27.2545090180361, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17556745, Length=185, Percent_Identity=35.6756756756757, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=136, Percent_Identity=32.3529411764706, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17506493, Length=186, Percent_Identity=28.494623655914, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=491, Percent_Identity=27.0875763747454, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=363, Percent_Identity=26.9972451790634, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=176, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=31.6455696202532, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=31.6455696202532, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=502, Percent_Identity=28.0876494023904, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI221458488, Length=504, Percent_Identity=26.984126984127, Blast_Score=147, Evalue=3e-35,
Organism=Drosophila melanogaster, GI78706572, Length=174, Percent_Identity=31.6091954022989, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585709, Length=178, Percent_Identity=29.7752808988764, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585711, Length=178, Percent_Identity=29.7752808988764, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585713, Length=178, Percent_Identity=29.7752808988764, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60701; Mature: 60701

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAMPDTAHTNRPTLPPEIARRRTFAIISHPDAGKTTLTEKFLLYGGAIQMAGQVRAKGE
CCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCHHHCCCH
ARRTRSDFMQMEKDRGISVSASAMSFDFLTPDVNFRFNLVDTPGHSDFSEDTYRTLTAVD
HHHHHHHHHHHHHCCCCEEEECCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
AAIMVIDGAKGVESQTQKLFEVCRLRDLPILTFCNKMDRESRDTFEIIDEIQENLAIDVT
EEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEC
PASWPIGVGREFLGCYDLLHDRLELMDRADRNKVAETVSLQGLDDPALADHIPADLLDKL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCCHHHHHHH
REEVEMARELLPAMDPEAFQQGTLTPIWFGSAMNSFGVKELMDGIAEYGPEPQVQNADPR
HHHHHHHHHHHCCCCHHHHHCCCCCEEEECCHHHHCCHHHHHHHHHHHCCCCCCCCCCCC
QVAPDEKKVSGFVFKVQANMDPKHRDRVAFVRLVSGHFNRGMKLTHVRSKKPMAISNPVM
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCCEE
FLASDRELAEEAWAGDIIGIPNHGQLRIGDALTEGEALRFTGIPSFAPELLQSCRAGDPL
EEECCHHHHHHHCCCCEEECCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHCCCCCC
KAKHLDKALMQFAEEGAAKVFKPTFGSGFIVGVVGALQFEVLASRIELEYGLPVRFEASQ
CHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEHHH
FTSARWVHGDKDAVEAFANANKQHIATDNDGDPVYLTRLQWDIDRVARDYPDITLSATKE
CCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCCEEEEEEEHHHHHHHHCCCCEEEHHHHH
LMVER
HHCCC
>Mature Secondary Structure
MRAMPDTAHTNRPTLPPEIARRRTFAIISHPDAGKTTLTEKFLLYGGAIQMAGQVRAKGE
CCCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCHHHHCCHHHCCCH
ARRTRSDFMQMEKDRGISVSASAMSFDFLTPDVNFRFNLVDTPGHSDFSEDTYRTLTAVD
HHHHHHHHHHHHHCCCCEEEECCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
AAIMVIDGAKGVESQTQKLFEVCRLRDLPILTFCNKMDRESRDTFEIIDEIQENLAIDVT
EEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEC
PASWPIGVGREFLGCYDLLHDRLELMDRADRNKVAETVSLQGLDDPALADHIPADLLDKL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCCHHHHHHH
REEVEMARELLPAMDPEAFQQGTLTPIWFGSAMNSFGVKELMDGIAEYGPEPQVQNADPR
HHHHHHHHHHHCCCCHHHHHCCCCCEEEECCHHHHCCHHHHHHHHHHHCCCCCCCCCCCC
QVAPDEKKVSGFVFKVQANMDPKHRDRVAFVRLVSGHFNRGMKLTHVRSKKPMAISNPVM
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEECCCEE
FLASDRELAEEAWAGDIIGIPNHGQLRIGDALTEGEALRFTGIPSFAPELLQSCRAGDPL
EEECCHHHHHHHCCCCEEECCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHCCCCCC
KAKHLDKALMQFAEEGAAKVFKPTFGSGFIVGVVGALQFEVLASRIELEYGLPVRFEASQ
CHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCEEEEHHH
FTSARWVHGDKDAVEAFANANKQHIATDNDGDPVYLTRLQWDIDRVARDYPDITLSATKE
CCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCCEEEEEEEHHHHHHHHCCCCEEEHHHHH
LMVER
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]