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The map label for this gene is puuA [H]

Identifier: 89053616

GI number: 89053616

Start: 1085729

End: 1087090

Strand: Reverse

Name: puuA [H]

Synonym: Jann_1125

Alternate gene names: 89053616

Gene position: 1087090-1085729 (Counterclockwise)

Preceding gene: 89053617

Following gene: 89053615

Centisome position: 25.18

GC content: 63.14

Gene sequence:

>1362_bases
ATGAGCCAGTCAGACCTCCTCACCAAACTGCCGCAGATCGCGCGCGATTACCTGCAAGGCCGCACCTTGGATGAGGTCGA
ATGCATCGTCTCGGACCTCGCGGGCGTGGCCCGGGGCAAGGCGATGCCGGGCGCGAAATTCGCGCGGCAGACGCAGTTCT
ACCTGCCCAACTCGATCTTTTTCCAAACCATTACAGGCGATTGGGCCGATGATGTCGGCGATGGCTTCACCGAGCCGGAC
ATGATCCTGACGCCCGATTTCGACACCGCCCTGCCCGCGCCCTGGACCGCCGACTGGACGCTTCAGGTGATCCACGACAT
CCACGACCAGCAGGGTGATCCCGTCACGTTTGCCCCGCGCAACGTCCTCAAACGCGTGGTGGATCTGTACAACGCGCGCG
GCTGGCAGCCGATCGTGGCGCCCGAGATGGAATTCTACCTCGTCGCCCCCAACACCGACCCGGCCCGAGAGATCGAGCCG
CCCATGGGCCGGTCTGGCAGGCGCGCCGCGGCCCGGCAGGCCTATTCGATGTCGGCCGTGGATGAATATGGGCAAGTCAT
CGATGATATTTACGATTTCGCCGAAAGCATGGGCCTTGAGATCGACGGCATCCTGCAAGAAGGCGGAGCGGGGCAGATCG
AATTGAACCTGCGTCACGGCGATCCGGTGAAGCTGGCCGACGAGATGTTCTACTTCAAACGGATGATCCGGGAGGCCGCG
CTGCGCCACGACATGTACGCGACGTTCATGGCCAAACCCATCGCCGGAGAGCCGGGCAGCGCGATGCATATCCACCACTC
GGTCGTGGGCAGCGCCACGGGGGCCAACATCTTCTCCGATGCCGACGGTGCGGAAACCCCCGCCTTCGCCCATTTCATCG
GCGGCATGCAGCGCAATATGCCCGCCGTGATCGCCCTGCTTGCGCCCTATGTGAACAGCTACCGCCGCTATGTCCCTGAT
TTTGCGGCACCAATCAACCTTGAATGGGGCCGCGACAATCGCACCACGGGGTTGCGGGTGCCGGTCTCGGAACCCTCTGC
ACGGCGGGTCGAAAACCGTCTGCCAGGCATGGATTGCAACCCCTATCTGGGCATCGCCGCCAGCCTCGCCACGGGCTATC
TGGGGTTGATGGAGGGCGAGGGTCCCTCGGATGAGTTCAAGGGCGACGCCTATTCGGCAGAGTCGGGCGTGCCACGGGGT
CTGGGCGACGCGCTGGGGTTGCTGGAGGCCAATCTGGGTGTCCAACAGGTGTTGGGAGAGGACTTCTGCCGCGTCTATTC
CGCCGTCAAGCAATTGGAATACAGCGAATTCCTCCACGTCATCAGCCCCTGGGAGCGGGAGCATCTGTTGCTGAATGTCT
GA

Upstream 100 bases:

>100_bases
CGCGCGCCAATGACAACCGCCTGATCGCCGATTGGCTGGCCGATGTGCTGGAAGGCGCGGACGCCACGCAGCCGCGCCGC
TTCAGGGTGGATGCCGACGC

Downstream 100 bases:

>100_bases
CCCGGGCTCCCTCCGCTGGTGGCAAGACACCGCGCTGATCACGGCTGGTGGCGTGGCGCTGGCGATCTGGCTTTGGGACG
GGCCGGTCATCTTCACCCCC

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSQSDLLTKLPQIARDYLQGRTLDEVECIVSDLAGVARGKAMPGAKFARQTQFYLPNSIFFQTITGDWADDVGDGFTEPD
MILTPDFDTALPAPWTADWTLQVIHDIHDQQGDPVTFAPRNVLKRVVDLYNARGWQPIVAPEMEFYLVAPNTDPAREIEP
PMGRSGRRAAARQAYSMSAVDEYGQVIDDIYDFAESMGLEIDGILQEGGAGQIELNLRHGDPVKLADEMFYFKRMIREAA
LRHDMYATFMAKPIAGEPGSAMHIHHSVVGSATGANIFSDADGAETPAFAHFIGGMQRNMPAVIALLAPYVNSYRRYVPD
FAAPINLEWGRDNRTTGLRVPVSEPSARRVENRLPGMDCNPYLGIAASLATGYLGLMEGEGPSDEFKGDAYSAESGVPRG
LGDALGLLEANLGVQQVLGEDFCRVYSAVKQLEYSEFLHVISPWEREHLLLNV

Sequences:

>Translated_453_residues
MSQSDLLTKLPQIARDYLQGRTLDEVECIVSDLAGVARGKAMPGAKFARQTQFYLPNSIFFQTITGDWADDVGDGFTEPD
MILTPDFDTALPAPWTADWTLQVIHDIHDQQGDPVTFAPRNVLKRVVDLYNARGWQPIVAPEMEFYLVAPNTDPAREIEP
PMGRSGRRAAARQAYSMSAVDEYGQVIDDIYDFAESMGLEIDGILQEGGAGQIELNLRHGDPVKLADEMFYFKRMIREAA
LRHDMYATFMAKPIAGEPGSAMHIHHSVVGSATGANIFSDADGAETPAFAHFIGGMQRNMPAVIALLAPYVNSYRRYVPD
FAAPINLEWGRDNRTTGLRVPVSEPSARRVENRLPGMDCNPYLGIAASLATGYLGLMEGEGPSDEFKGDAYSAESGVPRG
LGDALGLLEANLGVQQVLGEDFCRVYSAVKQLEYSEFLHVISPWEREHLLLNV
>Mature_452_residues
SQSDLLTKLPQIARDYLQGRTLDEVECIVSDLAGVARGKAMPGAKFARQTQFYLPNSIFFQTITGDWADDVGDGFTEPDM
ILTPDFDTALPAPWTADWTLQVIHDIHDQQGDPVTFAPRNVLKRVVDLYNARGWQPIVAPEMEFYLVAPNTDPAREIEPP
MGRSGRRAAARQAYSMSAVDEYGQVIDDIYDFAESMGLEIDGILQEGGAGQIELNLRHGDPVKLADEMFYFKRMIREAAL
RHDMYATFMAKPIAGEPGSAMHIHHSVVGSATGANIFSDADGAETPAFAHFIGGMQRNMPAVIALLAPYVNSYRRYVPDF
AAPINLEWGRDNRTTGLRVPVSEPSARRVENRLPGMDCNPYLGIAASLATGYLGLMEGEGPSDEFKGDAYSAESGVPRGL
GDALGLLEANLGVQQVLGEDFCRVYSAVKQLEYSEFLHVISPWEREHLLLNV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=445, Percent_Identity=24.2696629213483, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI87081870, Length=435, Percent_Identity=36.551724137931, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1790301, Length=415, Percent_Identity=30.3614457831325, Blast_Score=149, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 49879; Mature: 49748

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSDLLTKLPQIARDYLQGRTLDEVECIVSDLAGVARGKAMPGAKFARQTQFYLPNSIF
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEE
FQTITGDWADDVGDGFTEPDMILTPDFDTALPAPWTADWTLQVIHDIHDQQGDPVTFAPR
EEEECCCCHHHHCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECHH
NVLKRVVDLYNARGWQPIVAPEMEFYLVAPNTDPAREIEPPMGRSGRRAAARQAYSMSAV
HHHHHHHHHHHCCCCCCEECCCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHH
DEYGQVIDDIYDFAESMGLEIDGILQEGGAGQIELNLRHGDPVKLADEMFYFKRMIREAA
HHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LRHDMYATFMAKPIAGEPGSAMHIHHSVVGSATGANIFSDADGAETPAFAHFIGGMQRNM
HHHHHHHHHHHCCCCCCCCCEEEEHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCHHCCH
PAVIALLAPYVNSYRRYVPDFAAPINLEWGRDNRTTGLRVPVSEPSARRVENRLPGMDCN
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHCCCCCCCC
PYLGIAASLATGYLGLMEGEGPSDEFKGDAYSAESGVPRGLGDALGLLEANLGVQQVLGE
CHHHHHHHHHHHHHEEECCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
DFCRVYSAVKQLEYSEFLHVISPWEREHLLLNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
>Mature Secondary Structure 
SQSDLLTKLPQIARDYLQGRTLDEVECIVSDLAGVARGKAMPGAKFARQTQFYLPNSIF
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEE
FQTITGDWADDVGDGFTEPDMILTPDFDTALPAPWTADWTLQVIHDIHDQQGDPVTFAPR
EEEECCCCHHHHCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECHH
NVLKRVVDLYNARGWQPIVAPEMEFYLVAPNTDPAREIEPPMGRSGRRAAARQAYSMSAV
HHHHHHHHHHHCCCCCCEECCCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHH
DEYGQVIDDIYDFAESMGLEIDGILQEGGAGQIELNLRHGDPVKLADEMFYFKRMIREAA
HHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LRHDMYATFMAKPIAGEPGSAMHIHHSVVGSATGANIFSDADGAETPAFAHFIGGMQRNM
HHHHHHHHHHHCCCCCCCCCEEEEHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCHHCCH
PAVIALLAPYVNSYRRYVPDFAAPINLEWGRDNRTTGLRVPVSEPSARRVENRLPGMDCN
HHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHCCCCCCCC
PYLGIAASLATGYLGLMEGEGPSDEFKGDAYSAESGVPRGLGDALGLLEANLGVQQVLGE
CHHHHHHHHHHHHHEEECCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
DFCRVYSAVKQLEYSEFLHVISPWEREHLLLNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]