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The map label for this gene is gloA [H]

Identifier: 89053580

GI number: 89053580

Start: 1052388

End: 1052816

Strand: Reverse

Name: gloA [H]

Synonym: Jann_1089

Alternate gene names: 89053580

Gene position: 1052816-1052388 (Counterclockwise)

Preceding gene: 89053581

Following gene: 89053579

Centisome position: 24.38

GC content: 63.4

Gene sequence:

>429_bases
ATGACAATCCAATATCTACACACGATGGTGCGGGTCAAAGACCTCGACGCCTCGATCGCGTTTTTCCAGCTTCTGGGCCT
GGAGGAGACACGGCGCATGGACAGCGAGGGCGGGCGGTTCACGCTGGTGTTCATGACCCCGCCGGGGCAGGAGAATGCAT
CGGTGGAGCTGACGTACAACTGGGACGGGGACGAGGGCCTGCCGTCGGACAGCCGCCATTTCGGGCATCTGGCCTACCGC
GTCGATGATATCTACGGCCTGTGCCAGCATCTGCAGGACAACGGTGTCACGATCAACAGGCCACCGCGCGACGGGCATAT
GGCCTTCGTGCGCTCGCCCGACAATGTGTCGGTGGAACTGTTGCAGGCCGGCGAAGCGCTGGCCCCGGCGGAACCCTGGG
CGTCGATGGAAAACACGGGCCACTGGTGA

Upstream 100 bases:

>100_bases
GCCCGATCCAACCCACCCCACGCCGAATTGAAGAACAGGCGGGCTAGTCAAAGCCTGTCAGCGCGTTAGTCTATGCCCCG
AACTTAGGGGAGCTTTCGGC

Downstream 100 bases:

>100_bases
GGCCGGTGGCGCTCCGGCCCCTCCTCGGCGCTGCTGCCGCAGGCCTCCTCGCAGCCGCCCCGGTGGCCGCGCAGGATCAA
TGCCGGTTGGCGTTGCAGCT

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MTIQYLHTMVRVKDLDASIAFFQLLGLEETRRMDSEGGRFTLVFMTPPGQENASVELTYNWDGDEGLPSDSRHFGHLAYR
VDDIYGLCQHLQDNGVTINRPPRDGHMAFVRSPDNVSVELLQAGEALAPAEPWASMENTGHW

Sequences:

>Translated_142_residues
MTIQYLHTMVRVKDLDASIAFFQLLGLEETRRMDSEGGRFTLVFMTPPGQENASVELTYNWDGDEGLPSDSRHFGHLAYR
VDDIYGLCQHLQDNGVTINRPPRDGHMAFVRSPDNVSVELLQAGEALAPAEPWASMENTGHW
>Mature_141_residues
TIQYLHTMVRVKDLDASIAFFQLLGLEETRRMDSEGGRFTLVFMTPPGQENASVELTYNWDGDEGLPSDSRHFGHLAYRV
DDIYGLCQHLQDNGVTINRPPRDGHMAFVRSPDNVSVELLQAGEALAPAEPWASMENTGHW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=142, Percent_Identity=31.6901408450704, Blast_Score=67, Evalue=7e-12,
Organism=Escherichia coli, GI1787940, Length=131, Percent_Identity=34.3511450381679, Blast_Score=73, Evalue=7e-15,
Organism=Drosophila melanogaster, GI20129743, Length=150, Percent_Identity=32, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15977; Mature: 15846

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQYLHTMVRVKDLDASIAFFQLLGLEETRRMDSEGGRFTLVFMTPPGQENASVELTYN
CCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEE
WDGDEGLPSDSRHFGHLAYRVDDIYGLCQHLQDNGVTINRPPRDGHMAFVRSPDNVSVEL
CCCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCEEEE
LQAGEALAPAEPWASMENTGHW
EECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIQYLHTMVRVKDLDASIAFFQLLGLEETRRMDSEGGRFTLVFMTPPGQENASVELTYN
CHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEE
WDGDEGLPSDSRHFGHLAYRVDDIYGLCQHLQDNGVTINRPPRDGHMAFVRSPDNVSVEL
CCCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCCCEEEE
LQAGEALAPAEPWASMENTGHW
EECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]