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The map label for this gene is yogA [H]

Identifier: 89053284

GI number: 89053284

Start: 753467

End: 754774

Strand: Reverse

Name: yogA [H]

Synonym: Jann_0793

Alternate gene names: 89053284

Gene position: 754774-753467 (Counterclockwise)

Preceding gene: 89053285

Following gene: 89053283

Centisome position: 17.48

GC content: 63.76

Gene sequence:

>1308_bases
ATGACGGAGAGCACCATGGCCCTAGACGCGCCCACCACCGCCGCCCCTTACGACGCGCCCATGAAAGACCTCTACGAGAT
GGGCGAAATGCCCCCCATGGGCTACGTGCCGCCCAAAATGTACGCCTGGACCATCCGCCGCGAACGCCATGGCGAGCCGG
ACCAGGCCTTCAAGGTCGAAGTCGTCGATACCCCGGTCCTCGACAGCCACGAAGTGCTCGTCCTCGTGATGGCAGCGGGC
GTGAACTATAACGGCGTCTGGGCGGGTCTCGGCCAGCCGATCAGCCCCTTCGATGGCCACGGCGCGGACTACCACATTGC
GGGCTCGGACGCGTCCGGCATCGTGTGGGCCGTGGGCTCGGCGGTGAAGCGCTGGAAGGTGGGCGACGAGGTCGTGATCC
ACTGCAACCAGGACGACGGCGATGATGAACATTGCAACGGCGGCGACCCGATGTTCTCCACCTCCCAGCGGATCTGGGGC
TACGAGACGCCCGATGGCTCCTTCGCGCAGTTCACCAATGTGCAGGCCCAGCAGCTTATGCCGCGTCCCAAGCACCTGAC
GTGGGAAGAGGCCGCCTGCTACACCCTCACCCTGGCCACCGCCTACCGGATGCTGTTCGGGCACGAACCCCATGACCTGA
AGCCCGGCCAGAACGTCCTCGTCTGGGGCGCGTCGGGTGGCTTGGGTTCCTACGCGATCCAGCTGGCGAACACGGCGGGC
GCGAATGCCATTGCCGTCATCAGCGACGAATCCAAACGCGATTTCGTCCTGTCCCTCGGCGCGAAAGGCGTCATCAACCG
CAAGGACTTCAACTGTTGGGGTCAGCTGCCCACGGTCAACACGCCGGAATATGCGGAATGGTTCAAGGAAGCGCGCAAGT
TCGGCAAGTCGATCTGGGACATAACCGGCAAGGGCGTGAACGTCGACATGGTGTTTGAACACCCCGGCGAGGCAACCTTT
CCCGTCTCCACCCTCGTCTGCAAAAAAGGTGGCATGGTCGTGATCTGCGCGGGCACCACGGGCTTCAACTGCACCTTCGA
TGTGCGCTACATGTGGATGCACCAGAAGCGCTTGCAAGGCTCCCACTTCGCCCACCTCAAACAGGCGTCCGCCGCCAATG
ACCTGATGGTGCAGCAGCGCCTCGACCCCTGCATGTCCGAGGTTTTCCCCTGGGATGAGCTGCCCGGGGCCCACACCAAA
ATGCTCCGCAACGAACACAAGCCCGGCAATATGTCCGTGCTGGTCCAGGCTCCCCGCACCGGCCTGCGCACGCTGGAAGA
TGTGCTGGAGGCCGGGCCAAAAACCTGA

Upstream 100 bases:

>100_bases
AAACAAGCCTTTAAGGGTTGCAATATTACTCACCCGCCAATATCCCTCGCATCAGCAGCATTTGATTCCGCAGCGCGGCA
TCAACGCCCCGGAGGACCAC

Downstream 100 bases:

>100_bases
CATCGGCCACGCGCAGTTCAAGTGACCTCGCGTCATGACCCCGTGACGTGAGGTCGTTTGCCTGCAAACGCCCCTCCCAG
CGCTTGACCCACCACATTTG

Product: crotonyl-CoA reductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTESTMALDAPTTAAPYDAPMKDLYEMGEMPPMGYVPPKMYAWTIRRERHGEPDQAFKVEVVDTPVLDSHEVLVLVMAAG
VNYNGVWAGLGQPISPFDGHGADYHIAGSDASGIVWAVGSAVKRWKVGDEVVIHCNQDDGDDEHCNGGDPMFSTSQRIWG
YETPDGSFAQFTNVQAQQLMPRPKHLTWEEAACYTLTLATAYRMLFGHEPHDLKPGQNVLVWGASGGLGSYAIQLANTAG
ANAIAVISDESKRDFVLSLGAKGVINRKDFNCWGQLPTVNTPEYAEWFKEARKFGKSIWDITGKGVNVDMVFEHPGEATF
PVSTLVCKKGGMVVICAGTTGFNCTFDVRYMWMHQKRLQGSHFAHLKQASAANDLMVQQRLDPCMSEVFPWDELPGAHTK
MLRNEHKPGNMSVLVQAPRTGLRTLEDVLEAGPKT

Sequences:

>Translated_435_residues
MTESTMALDAPTTAAPYDAPMKDLYEMGEMPPMGYVPPKMYAWTIRRERHGEPDQAFKVEVVDTPVLDSHEVLVLVMAAG
VNYNGVWAGLGQPISPFDGHGADYHIAGSDASGIVWAVGSAVKRWKVGDEVVIHCNQDDGDDEHCNGGDPMFSTSQRIWG
YETPDGSFAQFTNVQAQQLMPRPKHLTWEEAACYTLTLATAYRMLFGHEPHDLKPGQNVLVWGASGGLGSYAIQLANTAG
ANAIAVISDESKRDFVLSLGAKGVINRKDFNCWGQLPTVNTPEYAEWFKEARKFGKSIWDITGKGVNVDMVFEHPGEATF
PVSTLVCKKGGMVVICAGTTGFNCTFDVRYMWMHQKRLQGSHFAHLKQASAANDLMVQQRLDPCMSEVFPWDELPGAHTK
MLRNEHKPGNMSVLVQAPRTGLRTLEDVLEAGPKT
>Mature_434_residues
TESTMALDAPTTAAPYDAPMKDLYEMGEMPPMGYVPPKMYAWTIRRERHGEPDQAFKVEVVDTPVLDSHEVLVLVMAAGV
NYNGVWAGLGQPISPFDGHGADYHIAGSDASGIVWAVGSAVKRWKVGDEVVIHCNQDDGDDEHCNGGDPMFSTSQRIWGY
ETPDGSFAQFTNVQAQQLMPRPKHLTWEEAACYTLTLATAYRMLFGHEPHDLKPGQNVLVWGASGGLGSYAIQLANTAGA
NAIAVISDESKRDFVLSLGAKGVINRKDFNCWGQLPTVNTPEYAEWFKEARKFGKSIWDITGKGVNVDMVFEHPGEATFP
VSTLVCKKGGMVVICAGTTGFNCTFDVRYMWMHQKRLQGSHFAHLKQASAANDLMVQQRLDPCMSEVFPWDELPGAHTKM
LRNEHKPGNMSVLVQAPRTGLRTLEDVLEAGPKT

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=364, Percent_Identity=22.5274725274725, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI13236495, Length=364, Percent_Identity=22.5274725274725, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI194239676, Length=224, Percent_Identity=25.8928571428571, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1790485, Length=371, Percent_Identity=24.2587601078167, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507255, Length=387, Percent_Identity=24.8062015503876, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71988145, Length=229, Percent_Identity=29.2576419213974, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323961, Length=301, Percent_Identity=23.9202657807309, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319621, Length=363, Percent_Identity=25.6198347107438, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324486, Length=375, Percent_Identity=24, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323729, Length=162, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 47838; Mature: 47707

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESTMALDAPTTAAPYDAPMKDLYEMGEMPPMGYVPPKMYAWTIRRERHGEPDQAFKVE
CCCCCEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEEE
VVDTPVLDSHEVLVLVMAAGVNYNGVWAGLGQPISPFDGHGADYHIAGSDASGIVWAVGS
EECCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEHHH
AVKRWKVGDEVVIHCNQDDGDDEHCNGGDPMFSTSQRIWGYETPDGSFAQFTNVQAQQLM
HHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHCCCCHHHHC
PRPKHLTWEEAACYTLTLATAYRMLFGHEPHDLKPGQNVLVWGASGGLGSYAIQLANTAG
CCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCC
ANAIAVISDESKRDFVLSLGAKGVINRKDFNCWGQLPTVNTPEYAEWFKEARKFGKSIWD
CCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
ITGKGVNVDMVFEHPGEATFPVSTLVCKKGGMVVICAGTTGFNCTFDVRYMWMHQKRLQG
CCCCCCEEEEEEECCCCCCCCHHEEEECCCCEEEEEECCCCCCEEEEEEEEEEHHHHHCC
SHFAHLKQASAANDLMVQQRLDPCMSEVFPWDELPGAHTKMLRNEHKPGNMSVLVQAPRT
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCH
GLRTLEDVLEAGPKT
HHHHHHHHHHCCCCC
>Mature Secondary Structure 
TESTMALDAPTTAAPYDAPMKDLYEMGEMPPMGYVPPKMYAWTIRRERHGEPDQAFKVE
CCCCEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEEE
VVDTPVLDSHEVLVLVMAAGVNYNGVWAGLGQPISPFDGHGADYHIAGSDASGIVWAVGS
EECCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEHHH
AVKRWKVGDEVVIHCNQDDGDDEHCNGGDPMFSTSQRIWGYETPDGSFAQFTNVQAQQLM
HHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHHHCCCCHHHHC
PRPKHLTWEEAACYTLTLATAYRMLFGHEPHDLKPGQNVLVWGASGGLGSYAIQLANTAG
CCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCC
ANAIAVISDESKRDFVLSLGAKGVINRKDFNCWGQLPTVNTPEYAEWFKEARKFGKSIWD
CCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
ITGKGVNVDMVFEHPGEATFPVSTLVCKKGGMVVICAGTTGFNCTFDVRYMWMHQKRLQG
CCCCCCEEEEEEECCCCCCCCHHEEEECCCCEEEEEECCCCCCEEEEEEEEEEHHHHHCC
SHFAHLKQASAANDLMVQQRLDPCMSEVFPWDELPGAHTKMLRNEHKPGNMSVLVQAPRT
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCH
GLRTLEDVLEAGPKT
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]