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The map label for this gene is dapE [H]

Identifier: 89053274

GI number: 89053274

Start: 744320

End: 745627

Strand: Reverse

Name: dapE [H]

Synonym: Jann_0783

Alternate gene names: 89053274

Gene position: 745627-744320 (Counterclockwise)

Preceding gene: 89053277

Following gene: 89053273

Centisome position: 17.27

GC content: 62.61

Gene sequence:

>1308_bases
TTGCGGCGAGGGGCCGTCAGCGCCACTCTGCGGGCCATGTCTGACACCAAACTTCTGGCCGCCGTGGCCGCCCGCCGGGA
CGCGCTGATTGCGCTGACCCAGGATCTGATCCGCATTCCCACCCTGAACCCACCGGGGCGTAAGTATTGGGAAATCTGCA
ATTATCTCGCGACGCGCCTGAAAGCGCAGGGATTTACGGTCGAGATGATCCGCGCCCATGGGGCCGTCGCCGACAGCGAC
ACCTATCCGCGCTGGAACATGGTCGCACGGCTGCAGCGGGGGGCCGGCGAGTGCGTGCATTTCAACAGCCACCACGATGT
CGTCGCCGTGGGTCATGGCTGGACCCGCGATCCGTTTGGCGGAGAGTTGGACGACGGCAAGATCTACGGGCGCGGGGCCT
GTGACATGAAAGGCGGGCTTGCGGCGTCGATCATCGCGGCGGAGGCGTTTATCGAGACCTATCCGGATTTCAACGGTGTG
ATTGAAATTTCGGCGACAGCAGATGAGGAATCCGGCGGCTATGGCGGCGTCGCCTATCTGGCGGAGCAGGGGTATTTCAG
CCCGGACCGCGTGCAGCACGTCATCATCCCCGAGCCGCTCAACAAGGATCGCATCTGTCTGGGCCATCGCGGCGTCTGGT
GGGCGGAGATCGAGACCCATGGCCGCATCGCCCACGGCTCCATGCCCTTCCTCGGCGATTGCGCCGTCCGCCACATGGGG
GCCGTTCTGGAAGAGATGGAGGCGTCGCTGTTCCCGCTTCTGGCCACGAAACGCACCGATATGCCCGTGGTGCCGGAAGG
AGCCAAGCAATCCACGATGAACATCAACGCCATCCACGGCGGAGAGCCGGTGCAGGACGCGGATTACACCGGCCTGCCTG
CGGCTTGCGTGCCAGATCGCTGCAAGATCACCATCGATCGCCGCTATCTTATAGAAGAAAATGAGGGCGACGTGCGGGCT
GAGATTATCGCTGTGCTGGAGAAGGTCAAAGCCGCCCGGCCCACGTTCCATTACGAGCTGAATGAGCTATGGAGCGTGTC
GCCCACCATGACGGACCGCGACGCGCCGGTGGTCAGGACGGTGGAGGCTGCCATCGCCAAGGTGCTGGAAACGCAGGCTG
AATTTGTCGTGTCCCCCGGCACTTACGACCAGAAACACATCGACCGGATCGGCACGTTGAAGAATTGCATCGCCTACGGG
CCGGGCATCCTTGACCTCGCCCACCAACCCGATGAATGGGTGGGCGTACAGGATATGGAAGACAGCGCCAAGGTCATGGC
GCTGACGCTTGCGGAGCTTCTTTTATAA

Upstream 100 bases:

>100_bases
CATCACAATCGCCGCCCGGCCAATTCCAAACATGCGTTATCGCCTCCAATCCCGTGGTATCGCCCTCTATATAGGTCACC
TCGCGCGCGAATGGCAGGGC

Downstream 100 bases:

>100_bases
GATCGAGGCGATCAGGCGTATTCCGGAAATCCAAGGTGGCGCAGGGCGCGTGCGGCAAGCTGGCTCGCTGCCGCATCGCC
CGCGATCAACACCTCGTTGA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MRRGAVSATLRAMSDTKLLAAVAARRDALIALTQDLIRIPTLNPPGRKYWEICNYLATRLKAQGFTVEMIRAHGAVADSD
TYPRWNMVARLQRGAGECVHFNSHHDVVAVGHGWTRDPFGGELDDGKIYGRGACDMKGGLAASIIAAEAFIETYPDFNGV
IEISATADEESGGYGGVAYLAEQGYFSPDRVQHVIIPEPLNKDRICLGHRGVWWAEIETHGRIAHGSMPFLGDCAVRHMG
AVLEEMEASLFPLLATKRTDMPVVPEGAKQSTMNINAIHGGEPVQDADYTGLPAACVPDRCKITIDRRYLIEENEGDVRA
EIIAVLEKVKAARPTFHYELNELWSVSPTMTDRDAPVVRTVEAAIAKVLETQAEFVVSPGTYDQKHIDRIGTLKNCIAYG
PGILDLAHQPDEWVGVQDMEDSAKVMALTLAELLL

Sequences:

>Translated_435_residues
MRRGAVSATLRAMSDTKLLAAVAARRDALIALTQDLIRIPTLNPPGRKYWEICNYLATRLKAQGFTVEMIRAHGAVADSD
TYPRWNMVARLQRGAGECVHFNSHHDVVAVGHGWTRDPFGGELDDGKIYGRGACDMKGGLAASIIAAEAFIETYPDFNGV
IEISATADEESGGYGGVAYLAEQGYFSPDRVQHVIIPEPLNKDRICLGHRGVWWAEIETHGRIAHGSMPFLGDCAVRHMG
AVLEEMEASLFPLLATKRTDMPVVPEGAKQSTMNINAIHGGEPVQDADYTGLPAACVPDRCKITIDRRYLIEENEGDVRA
EIIAVLEKVKAARPTFHYELNELWSVSPTMTDRDAPVVRTVEAAIAKVLETQAEFVVSPGTYDQKHIDRIGTLKNCIAYG
PGILDLAHQPDEWVGVQDMEDSAKVMALTLAELLL
>Mature_435_residues
MRRGAVSATLRAMSDTKLLAAVAARRDALIALTQDLIRIPTLNPPGRKYWEICNYLATRLKAQGFTVEMIRAHGAVADSD
TYPRWNMVARLQRGAGECVHFNSHHDVVAVGHGWTRDPFGGELDDGKIYGRGACDMKGGLAASIIAAEAFIETYPDFNGV
IEISATADEESGGYGGVAYLAEQGYFSPDRVQHVIIPEPLNKDRICLGHRGVWWAEIETHGRIAHGSMPFLGDCAVRHMG
AVLEEMEASLFPLLATKRTDMPVVPEGAKQSTMNINAIHGGEPVQDADYTGLPAACVPDRCKITIDRRYLIEENEGDVRA
EIIAVLEKVKAARPTFHYELNELWSVSPTMTDRDAPVVRTVEAAIAKVLETQAEFVVSPGTYDQKHIDRIGTLKNCIAYG
PGILDLAHQPDEWVGVQDMEDSAKVMALTLAELLL

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=419, Percent_Identity=27.2076372315036, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790395, Length=378, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789236, Length=344, Percent_Identity=25.2906976744186, Blast_Score=69, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24649206, Length=308, Percent_Identity=23.3766233766234, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 47630; Mature: 47630

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRGAVSATLRAMSDTKLLAAVAARRDALIALTQDLIRIPTLNPPGRKYWEICNYLATRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
KAQGFTVEMIRAHGAVADSDTYPRWNMVARLQRGAGECVHFNSHHDVVAVGHGWTRDPFG
HCCCCEEEEEHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCCCCC
GELDDGKIYGRGACDMKGGLAASIIAAEAFIETYPDFNGVIEISATADEESGGYGGVAYL
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHE
AEQGYFSPDRVQHVIIPEPLNKDRICLGHRGVWWAEIETHGRIAHGSMPFLGDCAVRHMG
ECCCCCCHHHEEEEECCCCCCCCCEEECCCCEEEEEEECCCEEECCCCCCHHHHHHHHHH
AVLEEMEASLFPLLATKRTDMPVVPEGAKQSTMNINAIHGGEPVQDADYTGLPAACVPDR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
CKITIDRRYLIEENEGDVRAEIIAVLEKVKAARPTFHYELNELWSVSPTMTDRDAPVVRT
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHEECCCCCCCCCCCHHHH
VEAAIAKVLETQAEFVVSPGTYDQKHIDRIGTLKNCIAYGPGILDLAHQPDEWVGVQDME
HHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCC
DSAKVMALTLAELLL
CHHHHHHHHHHHHHC
>Mature Secondary Structure
MRRGAVSATLRAMSDTKLLAAVAARRDALIALTQDLIRIPTLNPPGRKYWEICNYLATRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
KAQGFTVEMIRAHGAVADSDTYPRWNMVARLQRGAGECVHFNSHHDVVAVGHGWTRDPFG
HCCCCEEEEEHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCCCCC
GELDDGKIYGRGACDMKGGLAASIIAAEAFIETYPDFNGVIEISATADEESGGYGGVAYL
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHE
AEQGYFSPDRVQHVIIPEPLNKDRICLGHRGVWWAEIETHGRIAHGSMPFLGDCAVRHMG
ECCCCCCHHHEEEEECCCCCCCCCEEECCCCEEEEEEECCCEEECCCCCCHHHHHHHHHH
AVLEEMEASLFPLLATKRTDMPVVPEGAKQSTMNINAIHGGEPVQDADYTGLPAACVPDR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
CKITIDRRYLIEENEGDVRAEIIAVLEKVKAARPTFHYELNELWSVSPTMTDRDAPVVRT
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHEECCCCCCCCCCCHHHH
VEAAIAKVLETQAEFVVSPGTYDQKHIDRIGTLKNCIAYGPGILDLAHQPDEWVGVQDME
HHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCC
DSAKVMALTLAELLL
CHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA