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The map label for this gene is paaF [C]

Identifier: 89053156

GI number: 89053156

Start: 635820

End: 636572

Strand: Direct

Name: paaF [C]

Synonym: Jann_0665

Alternate gene names: 89053156

Gene position: 635820-636572 (Clockwise)

Preceding gene: 89053155

Following gene: 89053157

Centisome position: 14.72

GC content: 65.47

Gene sequence:

>753_bases
ATGAGCTTACTGGTCGAGATTGATGGGCGCATCGCGACGCTGACGCTGAACCGCCCCGAAAAGCGCAACGCGATGAATGA
TGCATTGATGGCGGAATTGTCCGCGTTTTGCGATGCCCCGCCCGAGGGCGTGCGCGTGATCATCCTAACGGGGGCGGGCG
GGCATTTTTGCGCGGGCCTTGATCTTGCGGAGCAGCATGACCGGGATCCGGCGGAGGCGATGGCCCATTCGCGCGGCTGG
CACCGGGCGCTGGAGCGGGTGCAGTTTGGCGGCCTGCCGGTCGTCGCCGCGATGTCCGGCGCGGTGATTGGGGGCGGTCT
GGAAGTTGCCTGTGCGGCCCATGTCCGGGTGGCGGGGCCTGACATGCGCTATCAGCTGCCCGAGGGACAACGCGGATTTT
TCGTGGGCGGCGGCGCGACGGTGCGTGTGGGCAGGATCATCGGGCCGGATCGGTTGACCGAGATGATGTTAACGGGCCGG
AGTTATGGCGCCGAGGAAGGCCTGGCCCTCGGGCTGTCGCATTATGTGGACGACGACCCGATGGCCAGGGCGCGGGTATT
GGCGGAGCAGATCGCGTCGAACGCGCAGATGTCGAATTATTTCGCGATCCATGGCGTGGCCCGGATCAACGCGATGGCCC
CGGAGGAGGGGTTCTTTGCGGAGGCCATGTGCTCCGCCCTTGCCCAGACTTCTCCGGACGCGAAGGAAGGGTTCGCCGCC
TTTCTTGAGAAAAGAGCGCCCCGGTTTGGCTGA

Upstream 100 bases:

>100_bases
ACCCGGCAGAGTTTGGGCAGATGGTCGCCAGTATCGTGGAAAACCCGTATCTGAACGGCGAAGTGATCCGCCTGGACGGC
GCCACACGGCTGCCGCCGAA

Downstream 100 bases:

>100_bases
TCGTGACGCGGATCTGCGGGCTCTGGTGAGCTACAACGTGAAGCGGGCGAATTCGGCCCTGATGGGGGATGTGGAACGCC
TAGTGGGGCGGTTCGGCTTG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSLLVEIDGRIATLTLNRPEKRNAMNDALMAELSAFCDAPPEGVRVIILTGAGGHFCAGLDLAEQHDRDPAEAMAHSRGW
HRALERVQFGGLPVVAAMSGAVIGGGLEVACAAHVRVAGPDMRYQLPEGQRGFFVGGGATVRVGRIIGPDRLTEMMLTGR
SYGAEEGLALGLSHYVDDDPMARARVLAEQIASNAQMSNYFAIHGVARINAMAPEEGFFAEAMCSALAQTSPDAKEGFAA
FLEKRAPRFG

Sequences:

>Translated_250_residues
MSLLVEIDGRIATLTLNRPEKRNAMNDALMAELSAFCDAPPEGVRVIILTGAGGHFCAGLDLAEQHDRDPAEAMAHSRGW
HRALERVQFGGLPVVAAMSGAVIGGGLEVACAAHVRVAGPDMRYQLPEGQRGFFVGGGATVRVGRIIGPDRLTEMMLTGR
SYGAEEGLALGLSHYVDDDPMARARVLAEQIASNAQMSNYFAIHGVARINAMAPEEGFFAEAMCSALAQTSPDAKEGFAA
FLEKRAPRFG
>Mature_249_residues
SLLVEIDGRIATLTLNRPEKRNAMNDALMAELSAFCDAPPEGVRVIILTGAGGHFCAGLDLAEQHDRDPAEAMAHSRGWH
RALERVQFGGLPVVAAMSGAVIGGGLEVACAAHVRVAGPDMRYQLPEGQRGFFVGGGATVRVGRIIGPDRLTEMMLTGRS
YGAEEGLALGLSHYVDDDPMARARVLAEQIASNAQMSNYFAIHGVARINAMAPEEGFFAEAMCSALAQTSPDAKEGFAAF
LEKRAPRFG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=30.241935483871, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI20127408, Length=173, Percent_Identity=30.635838150289, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI70995211, Length=173, Percent_Identity=30.0578034682081, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI4502327, Length=248, Percent_Identity=29.8387096774194, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI68989263, Length=182, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=25.8823529411765, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=33.0677290836653, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=29.3436293436293, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=185, Percent_Identity=33.5135135135135, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1790281, Length=167, Percent_Identity=29.3413173652695, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788597, Length=247, Percent_Identity=27.9352226720648, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=30.6122448979592, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=188, Percent_Identity=29.2553191489362, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=29.0983606557377, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=212, Percent_Identity=30.188679245283, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=182, Percent_Identity=32.967032967033, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144276, Length=162, Percent_Identity=32.7160493827161, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=162, Percent_Identity=32.7160493827161, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=175, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=162, Percent_Identity=32.7160493827161, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=189, Percent_Identity=28.042328042328, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=31.4741035856574, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=30.4878048780488, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=30.4878048780488, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21357171, Length=188, Percent_Identity=29.2553191489362, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920382, Length=198, Percent_Identity=28.2828282828283, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26554; Mature: 26422

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLVEIDGRIATLTLNRPEKRNAMNDALMAELSAFCDAPPEGVRVIILTGAGGHFCAGL
CEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECC
DLAEQHDRDPAEAMAHSRGWHRALERVQFGGLPVVAAMSGAVIGGGLEVACAAHVRVAGP
CHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCCEEECCCEEEEEEEEEEECCC
DMRYQLPEGQRGFFVGGGATVRVGRIIGPDRLTEMMLTGRSYGAEEGLALGLSHYVDDDP
CCEEECCCCCCCEEEECCCEEEEEEEECHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCH
MARARVLAEQIASNAQMSNYFAIHGVARINAMAPEEGFFAEAMCSALAQTSPDAKEGFAA
HHHHHHHHHHHHCCCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
FLEKRAPRFG
HHHHCCCCCC
>Mature Secondary Structure 
SLLVEIDGRIATLTLNRPEKRNAMNDALMAELSAFCDAPPEGVRVIILTGAGGHFCAGL
EEEEEECCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECC
DLAEQHDRDPAEAMAHSRGWHRALERVQFGGLPVVAAMSGAVIGGGLEVACAAHVRVAGP
CHHHHCCCCHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCCEEECCCEEEEEEEEEEECCC
DMRYQLPEGQRGFFVGGGATVRVGRIIGPDRLTEMMLTGRSYGAEEGLALGLSHYVDDDP
CCEEECCCCCCCEEEECCCEEEEEEEECHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCH
MARARVLAEQIASNAQMSNYFAIHGVARINAMAPEEGFFAEAMCSALAQTSPDAKEGFAA
HHHHHHHHHHHHCCCCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
FLEKRAPRFG
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA