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The map label for this gene is pcaF [H]

Identifier: 89053141

GI number: 89053141

Start: 621182

End: 622387

Strand: Direct

Name: pcaF [H]

Synonym: Jann_0650

Alternate gene names: 89053141

Gene position: 621182-622387 (Clockwise)

Preceding gene: 89053140

Following gene: 89053149

Centisome position: 14.39

GC content: 67.5

Gene sequence:

>1206_bases
ATGGCCGAGGCATTTATCTGCGATTACATCCGCACACCGATTGGACGGTTCGGTGGCGCGTTGGCCAAGGTGCGCGCCGA
TGACCTGGGCGCGGTGCCGCTGGCGGCGCTGATGGCGCGGAATTCAGGCGTGGATTGGGCGGCGGTGGACGACATTTACT
ATGGCTGCGCCAACCAGGCGGGCGAGGATAACCGCAACGTGGCGCGCATGTCGGCGCTTCTGGCGGGCTTGCCGGAGGCG
GTGCCGGGGACCACGATCAACCGCCTGTGCGGCTCTGGCATGGACGCGATCATCGCAGCGGCGCGGGCGATCAAGGCGGG
CGAGACGGAGTTGATGATTGCCGGCGGGGTCGAAAGCATGTCCCGCGCGCCCTTCGTGATGCCCAAGGCGGACACGGCGT
TTTCCCGCAAGGCCGAGATCTACGACACGACTATCGGCTGGCGCTTCGTGAACCCGGTGATGAAGGCGCAATACGGGATC
GAGTCCATGCCAGAGACGGCAGAGAATGTGGCCGAAGACTACACCGTGTCCCGCGCGGATCAGGATGCGTTTGCCGCGCG
GTCGCAAGCCAAGGCCGGGGCGGCCATGGCGTCGGGGCGGTTGGCGGAGGAAATCACGCCGGTGACGATCCCGCGTCGCA
AGGGCGAGCCTGTGGTTGTCTCTGAGGATGAGCACCCGAGGCCGGACACGGACGTGGCCGCGCTGTCGAAGTTGCGCGCG
CCGTTTCGGGATGGGGGCAGTGTGACGGCGGGCAATGCGTCCGGGGTGAACGACGGGGCAGCGGCGCTGGTGATCGCGTC
AGAGGCGGCTGCGGTGACGCATGGATTGACGCCCATCGCGCGGATTGTGGGCGGGGCCGTGGCAGGCGTGGCCCCCCGGA
TCATGGGGATCGGGCCGCTCCCCGCCTCGCAAAAGCTGATGGCGCGCCTGGGCCTGACCATTAGCGATTTCGACGTGATC
GAGTTGAACGAGGCCTTCGCGGCGCAGGGTCTGGCCGTCACGCGGGGCCTTGGGATTGCTGACGATGATGCCCGTGTGAA
TCCCAATGGCGGCGCGATAGCCCTGGGGCATCCCTTGGGGATGTCGGGCGCCCGCATCGCCGGATCTGCCGCGCTGGAGA
TGAAGTTGCGTGGTGGCACGCGCGCCTTGGCGACGATGTGCATCGGCGTGGGTCAGGGGATCGCTGTGGCGCTGGAGGCG
GTTTGA

Upstream 100 bases:

>100_bases
AATTTGCGTTACCGCGCGGCGGGTGCAATGCGGGCTGTGTAACGCTTTCCTCCCGATGACGGGCCTGATAGCGTCCCGCT
TGCAAATTGGGAGGACGACT

Downstream 100 bases:

>100_bases
TCCGGATGCGGTCGCGCGGGTGGCGCCTTCGGGACGCTCAGCGGGAGTTTTATGGGCCAAGATGAAGGAGCGGGAGCTTT
AGTGGTCCTGGTATTCCACT

Product: Beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAEAFICDYIRTPIGRFGGALAKVRADDLGAVPLAALMARNSGVDWAAVDDIYYGCANQAGEDNRNVARMSALLAGLPEA
VPGTTINRLCGSGMDAIIAAARAIKAGETELMIAGGVESMSRAPFVMPKADTAFSRKAEIYDTTIGWRFVNPVMKAQYGI
ESMPETAENVAEDYTVSRADQDAFAARSQAKAGAAMASGRLAEEITPVTIPRRKGEPVVVSEDEHPRPDTDVAALSKLRA
PFRDGGSVTAGNASGVNDGAAALVIASEAAAVTHGLTPIARIVGGAVAGVAPRIMGIGPLPASQKLMARLGLTISDFDVI
ELNEAFAAQGLAVTRGLGIADDDARVNPNGGAIALGHPLGMSGARIAGSAALEMKLRGGTRALATMCIGVGQGIAVALEA
V

Sequences:

>Translated_401_residues
MAEAFICDYIRTPIGRFGGALAKVRADDLGAVPLAALMARNSGVDWAAVDDIYYGCANQAGEDNRNVARMSALLAGLPEA
VPGTTINRLCGSGMDAIIAAARAIKAGETELMIAGGVESMSRAPFVMPKADTAFSRKAEIYDTTIGWRFVNPVMKAQYGI
ESMPETAENVAEDYTVSRADQDAFAARSQAKAGAAMASGRLAEEITPVTIPRRKGEPVVVSEDEHPRPDTDVAALSKLRA
PFRDGGSVTAGNASGVNDGAAALVIASEAAAVTHGLTPIARIVGGAVAGVAPRIMGIGPLPASQKLMARLGLTISDFDVI
ELNEAFAAQGLAVTRGLGIADDDARVNPNGGAIALGHPLGMSGARIAGSAALEMKLRGGTRALATMCIGVGQGIAVALEA
V
>Mature_400_residues
AEAFICDYIRTPIGRFGGALAKVRADDLGAVPLAALMARNSGVDWAAVDDIYYGCANQAGEDNRNVARMSALLAGLPEAV
PGTTINRLCGSGMDAIIAAARAIKAGETELMIAGGVESMSRAPFVMPKADTAFSRKAEIYDTTIGWRFVNPVMKAQYGIE
SMPETAENVAEDYTVSRADQDAFAARSQAKAGAAMASGRLAEEITPVTIPRRKGEPVVVSEDEHPRPDTDVAALSKLRAP
FRDGGSVTAGNASGVNDGAAALVIASEAAAVTHGLTPIARIVGGAVAGVAPRIMGIGPLPASQKLMARLGLTISDFDVIE
LNEAFAAQGLAVTRGLGIADDDARVNPNGGAIALGHPLGMSGARIAGSAALEMKLRGGTRALATMCIGVGQGIAVALEAV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=397, Percent_Identity=41.8136020151133, Blast_Score=281, Evalue=5e-76,
Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=39.6419437340153, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4557237, Length=403, Percent_Identity=38.2133995037221, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=38.6363636363636, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=30.0699300699301, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI194353979, Length=287, Percent_Identity=29.9651567944251, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=66.3341645885287, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1788554, Length=401, Percent_Identity=46.8827930174564, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI87082165, Length=399, Percent_Identity=44.6115288220551, Blast_Score=290, Evalue=9e-80,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=45.2970297029703, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1788683, Length=425, Percent_Identity=32.4705882352941, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=43.640897755611, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17535921, Length=407, Percent_Identity=37.1007371007371, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI25147385, Length=395, Percent_Identity=34.4303797468354, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17551802, Length=432, Percent_Identity=29.6296296296296, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=29.5285359801489, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325229, Length=405, Percent_Identity=36.0493827160494, Blast_Score=206, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6322031, Length=401, Percent_Identity=35.1620947630923, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24655093, Length=399, Percent_Identity=42.6065162907268, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI17648125, Length=397, Percent_Identity=39.5465994962217, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24640423, Length=407, Percent_Identity=35.6265356265356, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=29.4811320754717, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41068; Mature: 40937

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAFICDYIRTPIGRFGGALAKVRADDLGAVPLAALMARNSGVDWAAVDDIYYGCANQA
CCCHHHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHCCCCC
GEDNRNVARMSALLAGLPEAVPGTTINRLCGSGMDAIIAAARAIKAGETELMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCHHHH
SRAPFVMPKADTAFSRKAEIYDTTIGWRFVNPVMKAQYGIESMPETAENVAEDYTVSRAD
CCCCEECCCCCCHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
QDAFAARSQAKAGAAMASGRLAEEITPVTIPRRKGEPVVVSEDEHPRPDTDVAALSKLRA
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCC
PFRDGGSVTAGNASGVNDGAAALVIASEAAAVTHGLTPIARIVGGAVAGVAPRIMGIGPL
CCCCCCCEECCCCCCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCC
PASQKLMARLGLTISDFDVIELNEAFAAQGLAVTRGLGIADDDARVNPNGGAIALGHPLG
CHHHHHHHHHCCEECCCCEEECCHHHHHCCHHHHCCCCCCCCCCEECCCCCEEEECCCCC
MSGARIAGSAALEMKLRGGTRALATMCIGVGQGIAVALEAV
CCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
AEAFICDYIRTPIGRFGGALAKVRADDLGAVPLAALMARNSGVDWAAVDDIYYGCANQA
CCHHHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHCCCCC
GEDNRNVARMSALLAGLPEAVPGTTINRLCGSGMDAIIAAARAIKAGETELMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCHHHH
SRAPFVMPKADTAFSRKAEIYDTTIGWRFVNPVMKAQYGIESMPETAENVAEDYTVSRAD
CCCCEECCCCCCHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
QDAFAARSQAKAGAAMASGRLAEEITPVTIPRRKGEPVVVSEDEHPRPDTDVAALSKLRA
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHCC
PFRDGGSVTAGNASGVNDGAAALVIASEAAAVTHGLTPIARIVGGAVAGVAPRIMGIGPL
CCCCCCCEECCCCCCCCCCCEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCC
PASQKLMARLGLTISDFDVIELNEAFAAQGLAVTRGLGIADDDARVNPNGGAIALGHPLG
CHHHHHHHHHCCEECCCCEEECCHHHHHCCHHHHCCCCCCCCCCEECCCCCEEEECCCCC
MSGARIAGSAALEMKLRGGTRALATMCIGVGQGIAVALEAV
CCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]