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The map label for this gene is rpoA

Identifier: 89053108

GI number: 89053108

Start: 590815

End: 591831

Strand: Direct

Name: rpoA

Synonym: Jann_0617

Alternate gene names: 89053108

Gene position: 590815-591831 (Clockwise)

Preceding gene: 89053107

Following gene: 89053109

Centisome position: 13.68

GC content: 59.39

Gene sequence:

>1017_bases
ATGATCCATAAAAATTGGCAAGAGCTGATCAAACCCACCCAGCTGGTCGTCCAGCCGGGCACTGATCCTGCGCGGAAAGC
CACCGTCGTGGCTGAACCGCTGGAGCGGGGCTTCGGCCTGACGCTTGGCAACGCGCTGCGGCGTGTTCTGATGTCCTCGC
TGCAAGGCGCGGCCATTACGTCGGTGCAGATTGACGGCGTGTTGCACGAATTCTCCAGCGTCGCCGGTGTGCGCGAAGAT
GTCACCGACATCGTGCTGAACCTCAAGGGTGTGGCAATCCGCATGGACGCCGAAGGCCCCAAGCGTGTTTCCATCTCCGC
CAAGGGCCCCCGGGTCGTGACGGCGGGTGACATCAGCGAGAGCGCAGGTATCGACGTCCTCAATAAGGATCACGTGATTT
GCCACCTCGACGACGGTGCTGACCTCTACATCGAGCTGACGGTCAACACCGGCAAAGGCTATGTGGCGGCTGACAAGAAC
CGCCCGGAAGATGCGCCGATTGGCCTGATGCCGATCGATGCGATCTATTCGCCGGTCAAGAAGGTGTCCTATGACGTGCA
GCCCACCCGTGAGGGGCAGGTGCTGGACTATGACAAGCTGACGCTGAAGCTGGAAACCGACGGCTCGCTGAGCCCTGACG
ATGCCGTGGCCTATGCCGCGCGGATCATCCAGGACCAGCTGTCGATCTTCGTGAACTTCGACGAGCCTGAGTCCGCGACG
CGTCAGGACGACGAAGACGATCTGGAATTCAACCCGCTTCTCCTGAAGAAGGTTGATGAGCTGGAGCTGTCCGTGCGGTC
GGCCAACTGCCTGAAGAACGACAATATCGTGTATATTGGCGATCTGATCCAGAAGACCGAAGCCGAGATGCTGCGCACGC
CGAACTTTGGCCGCAAGTCCTTGAACGAGATCAAGGAAGTGCTGTCCGGCATGGGCCTGCACCTTGGCATGGATATCGTG
GACTGGCCGCCCGACAACATCGAAGAGTTGGCCAAGAAGTACGAAGACAACTTCTAA

Upstream 100 bases:

>100_bases
CTACTTCTCCCCGCGTCATTCCAGGTGGCGCGGGGCGACGTCATTTTGAACCTCGAGCGTGTCGGACCCGGATCCCACCG
ACACAGGAATGGAGGGACGT

Downstream 100 bases:

>100_bases
GGTTTACGCGGGCGGGGGCTGACCTTCGCCCGCGACAATGCCCGGGTGAGCCGGGGAAAATATGGTTTCGCCGCGCTGAA
GCGCAGCTTTACCCAAGGAG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MIHKNWQELIKPTQLVVQPGTDPARKATVVAEPLERGFGLTLGNALRRVLMSSLQGAAITSVQIDGVLHEFSSVAGVRED
VTDIVLNLKGVAIRMDAEGPKRVSISAKGPRVVTAGDISESAGIDVLNKDHVICHLDDGADLYIELTVNTGKGYVAADKN
RPEDAPIGLMPIDAIYSPVKKVSYDVQPTREGQVLDYDKLTLKLETDGSLSPDDAVAYAARIIQDQLSIFVNFDEPESAT
RQDDEDDLEFNPLLLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLSGMGLHLGMDIV
DWPPDNIEELAKKYEDNF

Sequences:

>Translated_338_residues
MIHKNWQELIKPTQLVVQPGTDPARKATVVAEPLERGFGLTLGNALRRVLMSSLQGAAITSVQIDGVLHEFSSVAGVRED
VTDIVLNLKGVAIRMDAEGPKRVSISAKGPRVVTAGDISESAGIDVLNKDHVICHLDDGADLYIELTVNTGKGYVAADKN
RPEDAPIGLMPIDAIYSPVKKVSYDVQPTREGQVLDYDKLTLKLETDGSLSPDDAVAYAARIIQDQLSIFVNFDEPESAT
RQDDEDDLEFNPLLLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLSGMGLHLGMDIV
DWPPDNIEELAKKYEDNF
>Mature_338_residues
MIHKNWQELIKPTQLVVQPGTDPARKATVVAEPLERGFGLTLGNALRRVLMSSLQGAAITSVQIDGVLHEFSSVAGVRED
VTDIVLNLKGVAIRMDAEGPKRVSISAKGPRVVTAGDISESAGIDVLNKDHVICHLDDGADLYIELTVNTGKGYVAADKN
RPEDAPIGLMPIDAIYSPVKKVSYDVQPTREGQVLDYDKLTLKLETDGSLSPDDAVAYAARIIQDQLSIFVNFDEPESAT
RQDDEDDLEFNPLLLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLSGMGLHLGMDIV
DWPPDNIEELAKKYEDNF

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=307, Percent_Identity=47.8827361563518, Blast_Score=295, Evalue=2e-81,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_JANSC (Q28US8)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508559.1
- ProteinModelPortal:   Q28US8
- STRING:   Q28US8
- GeneID:   3933059
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0617
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q28US8
- ProtClustDB:   PRK05182
- BioCyc:   JSP290400:JANN_0617-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37151; Mature: 37151

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHKNWQELIKPTQLVVQPGTDPARKATVVAEPLERGFGLTLGNALRRVLMSSLQGAAIT
CCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEE
SVQIDGVLHEFSSVAGVREDVTDIVLNLKGVAIRMDAEGPKRVSISAKGPRVVTAGDISE
EEEHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCEEEEECCCCC
SAGIDVLNKDHVICHLDDGADLYIELTVNTGKGYVAADKNRPEDAPIGLMPIDAIYSPVK
CCCCEEECCCEEEEEECCCCEEEEEEEEECCCEEEEECCCCCCCCCCCEEEHHHHHHHHH
KVSYDVQPTREGQVLDYDKLTLKLETDGSLSPDDAVAYAARIIQDQLSIFVNFDEPESAT
HHCCCCCCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCC
RQDDEDDLEFNPLLLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKS
CCCCCCCCCCCCHHHHHHHHHHEEEHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHH
LNEIKEVLSGMGLHLGMDIVDWPPDNIEELAKKYEDNF
HHHHHHHHHCCCCEECCEEECCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIHKNWQELIKPTQLVVQPGTDPARKATVVAEPLERGFGLTLGNALRRVLMSSLQGAAIT
CCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEE
SVQIDGVLHEFSSVAGVREDVTDIVLNLKGVAIRMDAEGPKRVSISAKGPRVVTAGDISE
EEEHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCEEEEECCCCC
SAGIDVLNKDHVICHLDDGADLYIELTVNTGKGYVAADKNRPEDAPIGLMPIDAIYSPVK
CCCCEEECCCEEEEEECCCCEEEEEEEEECCCEEEEECCCCCCCCCCCEEEHHHHHHHHH
KVSYDVQPTREGQVLDYDKLTLKLETDGSLSPDDAVAYAARIIQDQLSIFVNFDEPESAT
HHCCCCCCCCCCCEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCC
RQDDEDDLEFNPLLLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKS
CCCCCCCCCCCCHHHHHHHHHHEEEHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHH
LNEIKEVLSGMGLHLGMDIVDWPPDNIEELAKKYEDNF
HHHHHHHHHCCCCEECCEEECCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA