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The map label for this gene is tuf1 [H]

Identifier: 89053069

GI number: 89053069

Start: 567648

End: 568823

Strand: Direct

Name: tuf1 [H]

Synonym: Jann_0578

Alternate gene names: 89053069

Gene position: 567648-568823 (Clockwise)

Preceding gene: 89053068

Following gene: 89053071

Centisome position: 13.15

GC content: 61.05

Gene sequence:

>1176_bases
ATGGCGAAGGAAAAGTTTGAGCGTTCTAAGCCGCATGTGAACATCGGTACGATTGGTCATGTTGACCATGGTAAGACGAC
GTTGACGGCGGCGATTACGAAGCAGTTTGGCGATTTTCAGGATTACGCGTCGATTGACTCGGCGCCTGAGGAGCGGGCGC
GGGGGATCACGATCTCGACGGCGCACGTGGAATACGAGACGGAAAACCGTCACTATGCCCACGTCGACTGCCCTGGCCAC
GCGGATTATGTGAAGAACATGATCACCGGTGCGGCGCAGATGGACGGCGCGATCCTGGTGGTGAACGCGGCCGATGGCCC
GATGCCCCAGACGCGCGAGCACATCCTGCTGGGTCGTCAGGTCGGCATTCCGTACATGGTTGTCTACATGAACAAGGTCG
ACCAGGTGGATGACGAGGAGCTGCTGGAGCTTGTCGAGATGGAAATTCGGGAATTGTTAAGCTCCTACGAGTATCCTGGC
GACGATATCCCTGTGATCCCCGGTTCGGCCCTGGCCGCTCTGGAAGGTCGTGACGATGCGATCGGCAAAGACTCCATCGA
CAAGCTGATGGCGGCCGTGGACGAGTATATCCCGACGCCCGCGCGCGCCGTGGATCAGCCGTTCCTGATGCCGATCGAGG
ACGTGTTCTCGATCTCGGGTCGGGGTACGGTTGTGACCGGTCGTGTGGAGCGTGGCGTGATCAACGTGGGCGACGAGATC
TCCATCGTCGGCATCCGCGACACCACGAAGACGACCTGCACGGGCGTTGAGATGTTCCGCAAGCTGCTGGATCGCGGCGA
GGCGGGCGACAATATCGGCGCGCTGCTGCGTGGTGTGGACCGTGAAGGTGTTGAGCGCGGGCAAGTTCTCTGCAAGCCCG
GCTCGGTTGACCCGCACACGAAGTTCGAGGCGGAGGCGTATATCCTGACGAAGGAAGAGGGTGGCCGTCACACGCCGTTC
TTCGCCAACTACCGCCCGCAATTCTACTTCCGCACGACGGATGTGACCGGCACGGTGAACCTGCCTGCGGGCACGGAAAT
GGTGATGCCCGGCGACAACCTGAAGTTCGAGGTCGAGCTGATCGCGCCGATCGCGATGGAAGACGGCCTGCGCTTCGCTA
TCCGCGAAGGCGGCCGGACGGTTGGTGCTGGTGTGGTGGCGAAGATCATCGAGTAA

Upstream 100 bases:

>100_bases
CGAGATCCAAGCGAAATACGCATAAAGCGGGGCGGATCATCCGCCCGCCCCAACCGTTGGGTCGCCGACACGGTGCCCAA
ACACTTTTAAGGAGGCCATC

Downstream 100 bases:

>100_bases
GGTCGTGTTTCCCTCGGAAACGATGGCCAGCGGGGAAAGGCGGTTTTGCTAAGGCAAAACCTGCCGACCCGCCCAGAAGA
AATTAGAAAAGGCCGCCCCA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAKEKFERSKPHVNIGTIGHVDHGKTTLTAAITKQFGDFQDYASIDSAPEERARGITISTAHVEYETENRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLGRQVGIPYMVVYMNKVDQVDDEELLELVEMEIRELLSSYEYPG
DDIPVIPGSALAALEGRDDAIGKDSIDKLMAAVDEYIPTPARAVDQPFLMPIEDVFSISGRGTVVTGRVERGVINVGDEI
SIVGIRDTTKTTCTGVEMFRKLLDRGEAGDNIGALLRGVDREGVERGQVLCKPGSVDPHTKFEAEAYILTKEEGGRHTPF
FANYRPQFYFRTTDVTGTVNLPAGTEMVMPGDNLKFEVELIAPIAMEDGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_391_residues
MAKEKFERSKPHVNIGTIGHVDHGKTTLTAAITKQFGDFQDYASIDSAPEERARGITISTAHVEYETENRHYAHVDCPGH
ADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLGRQVGIPYMVVYMNKVDQVDDEELLELVEMEIRELLSSYEYPG
DDIPVIPGSALAALEGRDDAIGKDSIDKLMAAVDEYIPTPARAVDQPFLMPIEDVFSISGRGTVVTGRVERGVINVGDEI
SIVGIRDTTKTTCTGVEMFRKLLDRGEAGDNIGALLRGVDREGVERGQVLCKPGSVDPHTKFEAEAYILTKEEGGRHTPF
FANYRPQFYFRTTDVTGTVNLPAGTEMVMPGDNLKFEVELIAPIAMEDGLRFAIREGGRTVGAGVVAKIIE
>Mature_390_residues
AKEKFERSKPHVNIGTIGHVDHGKTTLTAAITKQFGDFQDYASIDSAPEERARGITISTAHVEYETENRHYAHVDCPGHA
DYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLGRQVGIPYMVVYMNKVDQVDDEELLELVEMEIRELLSSYEYPGD
DIPVIPGSALAALEGRDDAIGKDSIDKLMAAVDEYIPTPARAVDQPFLMPIEDVFSISGRGTVVTGRVERGVINVGDEIS
IVGIRDTTKTTCTGVEMFRKLLDRGEAGDNIGALLRGVDREGVERGQVLCKPGSVDPHTKFEAEAYILTKEEGGRHTPFF
ANYRPQFYFRTTDVTGTVNLPAGTEMVMPGDNLKFEVELIAPIAMEDGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.3756345177665, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=30.8924485125858, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=30.8924485125858, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI4503475, Length=449, Percent_Identity=26.9487750556793, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI4503471, Length=444, Percent_Identity=27.9279279279279, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI194018522, Length=330, Percent_Identity=30.6060606060606, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194018520, Length=329, Percent_Identity=30.6990881458967, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194097354, Length=329, Percent_Identity=30.6990881458967, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI54607086, Length=313, Percent_Identity=27.7955271565495, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI46094014, Length=326, Percent_Identity=29.7546012269939, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=27.8810408921933, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI157426893, Length=266, Percent_Identity=26.6917293233083, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=33.5820895522388, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=35.7798165137615, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=35.7798165137615, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=35.7798165137615, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=74.1772151898734, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=74.1772151898734, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=374, Percent_Identity=29.6791443850267, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI48994988, Length=269, Percent_Identity=31.2267657992565, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789108, Length=261, Percent_Identity=33.7164750957854, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1788922, Length=144, Percent_Identity=32.6388888888889, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.8796992481203, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=42.3772609819121, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI17552884, Length=443, Percent_Identity=28.4424379232506, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=443, Percent_Identity=28.4424379232506, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=28.1395348837209, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=450, Percent_Identity=26, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=27.5, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=26.8965517241379, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=296, Percent_Identity=27.027027027027, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=36.3636363636364, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=276, Percent_Identity=27.1739130434783, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=230, Percent_Identity=26.9565217391304, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.1616161616162, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=28.3446712018141, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=28.3446712018141, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=449, Percent_Identity=24.7216035634744, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.3185840707965, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=28.3018867924528, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=187, Percent_Identity=30.4812834224599, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=26.6393442622951, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322359, Length=131, Percent_Identity=32.0610687022901, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.2697201017812, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.2697201017812, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=49.2227979274611, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=28.1318681318681, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=28.1318681318681, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=27.4725274725275, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=27.4725274725275, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=27.4725274725275, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=27.4725274725275, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45550900, Length=446, Percent_Identity=26.6816143497758, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI19922690, Length=300, Percent_Identity=29.6666666666667, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=29.8850574712644, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24647048, Length=277, Percent_Identity=27.7978339350181, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24647046, Length=277, Percent_Identity=27.7978339350181, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28572034, Length=158, Percent_Identity=36.0759493670886, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42739; Mature: 42608

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERSKPHVNIGTIGHVDHGKTTLTAAITKQFGDFQDYASIDSAPEERARGITIST
CCCHHHHHCCCCEEECEEECCCCCCEEHHHHHHHHHCCHHHHHHCCCCHHHHHCCEEEEE
AHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLGRQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHEEEECC
VGIPYMVVYMNKVDQVDDEELLELVEMEIRELLSSYEYPGDDIPVIPGSALAALEGRDDA
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCC
IGKDSIDKLMAAVDEYIPTPARAVDQPFLMPIEDVFSISGRGTVVTGRVERGVINVGDEI
CCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEEECCCEEECCCEE
SIVGIRDTTKTTCTGVEMFRKLLDRGEAGDNIGALLRGVDREGVERGQVLCKPGSVDPHT
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCCCCC
KFEAEAYILTKEEGGRHTPFFANYRPQFYFRTTDVTGTVNLPAGTEMVMPGDNLKFEVEL
CCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEEEEE
IAPIAMEDGLRFAIREGGRTVGAGVVAKIIE
EEEEECCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERSKPHVNIGTIGHVDHGKTTLTAAITKQFGDFQDYASIDSAPEERARGITIST
CCHHHHHCCCCEEECEEECCCCCCEEHHHHHHHHHCCHHHHHHCCCCHHHHHCCEEEEE
AHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLGRQ
EEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHEEEECC
VGIPYMVVYMNKVDQVDDEELLELVEMEIRELLSSYEYPGDDIPVIPGSALAALEGRDDA
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCC
IGKDSIDKLMAAVDEYIPTPARAVDQPFLMPIEDVFSISGRGTVVTGRVERGVINVGDEI
CCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEEEEECCCEEECCCEE
SIVGIRDTTKTTCTGVEMFRKLLDRGEAGDNIGALLRGVDREGVERGQVLCKPGSVDPHT
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEECCCCCCCCC
KFEAEAYILTKEEGGRHTPFFANYRPQFYFRTTDVTGTVNLPAGTEMVMPGDNLKFEVEL
CCCCEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCCEEEEEE
IAPIAMEDGLRFAIREGGRTVGAGVVAKIIE
EEEEECCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA