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The map label for this gene is greA

Identifier: 89052989

GI number: 89052989

Start: 496937

End: 497407

Strand: Direct

Name: greA

Synonym: Jann_0498

Alternate gene names: 89052989

Gene position: 496937-497407 (Clockwise)

Preceding gene: 89052988

Following gene: 89052990

Centisome position: 11.51

GC content: 56.26

Gene sequence:

>471_bases
ATGGAAAAGATCCCCATGACACCGAAAGGCCTGGAGGCCATGAACGGTGAACTCAAGCAGCTCAAGAGCGTTGAACGTCC
CGCGATCATCAAGGCGATTGCCGAGGCGCGAGAGCATGGGGACCTGTCGGAGAACGCTGAGTATCACTCCGCCAAGGAAA
AACAGTCCTTCATAGAGGGCCGGATCAAGGAGCTTGAGGGCTCTATTTCGCTGGCCCAGGTGATTGATCCCGCGACGCTG
TCTGGCGCGATCAAGTTCGGCGCCACCGTTGATCTTGTCGACGAAGAGACCGAAGAAGAAAAGACCTACATGATTGTTGG
TGAGGCCGAGGCCGATATCGAAAAGGGTCTGCTCAACATCAAATCCCCCCTCGCGCGCGCCTTGATCGGCAAGGAGGAGG
GCGATAGCGTTGAGGTTCGCACGCCCGGCGGCGCCAAAGGTTTTGAGATCGTCAAGATTTCTTATGTCTGA

Upstream 100 bases:

>100_bases
TTGATCCGGCGCGCGTTCGCGCCTAACCTCAAACGGTCCCGGCATCGCACAGGCGTTAGCCGGGTTTTCATTTGCTCCTG
TTCTTTAAAGGCACCGACCG

Downstream 100 bases:

>100_bases
GGCCTACGCCACGACCCTCTGTAACCTGGGGTGACACCAGACGATGACGTCCTCCTCTGAGCCCACATCATCTGCCCCGG
CGGCCCCCAAGGCCGCTCCG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MEKIPMTPKGLEAMNGELKQLKSVERPAIIKAIAEAREHGDLSENAEYHSAKEKQSFIEGRIKELEGSISLAQVIDPATL
SGAIKFGATVDLVDEETEEEKTYMIVGEAEADIEKGLLNIKSPLARALIGKEEGDSVEVRTPGGAKGFEIVKISYV

Sequences:

>Translated_156_residues
MEKIPMTPKGLEAMNGELKQLKSVERPAIIKAIAEAREHGDLSENAEYHSAKEKQSFIEGRIKELEGSISLAQVIDPATL
SGAIKFGATVDLVDEETEEEKTYMIVGEAEADIEKGLLNIKSPLARALIGKEEGDSVEVRTPGGAKGFEIVKISYV
>Mature_156_residues
MEKIPMTPKGLEAMNGELKQLKSVERPAIIKAIAEAREHGDLSENAEYHSAKEKQSFIEGRIKELEGSISLAQVIDPATL
SGAIKFGATVDLVDEETEEEKTYMIVGEAEADIEKGLLNIKSPLARALIGKEEGDSVEVRTPGGAKGFEIVKISYV

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=55.0632911392405, Blast_Score=177, Evalue=2e-46,
Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=33.974358974359, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_JANSC (Q28V47)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508440.1
- ProteinModelPortal:   Q28V47
- SMR:   Q28V47
- STRING:   Q28V47
- GeneID:   3932939
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0498
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- PhylomeDB:   Q28V47
- ProtClustDB:   PRK00226
- BioCyc:   JSP290400:JANN_0498-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 16911; Mature: 16911

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIPMTPKGLEAMNGELKQLKSVERPAIIKAIAEAREHGDLSENAEYHSAKEKQSFIEG
CCCCCCCCCCHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RIKELEGSISLAQVIDPATLSGAIKFGATVDLVDEETEEEKTYMIVGEAEADIEKGLLNI
HHHHHCCCCHHHHHHCCHHHCCEEECCCEEEECCCCCCCCCEEEEEECCHHHHHHHHHHH
KSPLARALIGKEEGDSVEVRTPGGAKGFEIVKISYV
HHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEC
>Mature Secondary Structure
MEKIPMTPKGLEAMNGELKQLKSVERPAIIKAIAEAREHGDLSENAEYHSAKEKQSFIEG
CCCCCCCCCCHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
RIKELEGSISLAQVIDPATLSGAIKFGATVDLVDEETEEEKTYMIVGEAEADIEKGLLNI
HHHHHCCCCHHHHHHCCHHHCCEEECCCEEEECCCCCCCCCEEEEEECCHHHHHHHHHHH
KSPLARALIGKEEGDSVEVRTPGGAKGFEIVKISYV
HHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA