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The map label for this gene is xthA [H]

Identifier: 89052912

GI number: 89052912

Start: 424521

End: 425309

Strand: Direct

Name: xthA [H]

Synonym: Jann_0421

Alternate gene names: 89052912

Gene position: 424521-425309 (Clockwise)

Preceding gene: 89052911

Following gene: 89052913

Centisome position: 9.83

GC content: 61.09

Gene sequence:

>789_bases
ATGCCCTTCACTCTCGCCACCTGGAACATCAACTCCGTCCGTCTGCGCCAACCGATCGTGTCGCAACTCATGACCGAGGA
GGCGCCCGACATTCTGTGCCTTCAGGAATGCAAAAGCCCCGTCGACAAGATGCCGTCCGATGTCTTTGCAGCGCTTGGCT
ACACCCATGTCGTGGCGCGTGGGCAGAAGGGCTACAACGGTGTGGCGATCTACTCCAAACTCCCGATGGAGGAGGCCGGA
TCCCATGAATTCGCAGGCCTCGACCATGCGCGCCACATCGCCGGGCGGCTGGAAAACGGCGTCACCATCCACAACTTCTA
TGTGCCCGCGGGCGGCGACGTGCCGGACCGCGACGTGAACATCAAGTTCGGCCAGAAGCTCGACTACCTCACCGATATGC
GAGATTGGGCGAAGACGGACACACCGCAGAAATCCATCCTCTTGGGCGACCTGAACATCGCACCCCGGGAAGATGACGTC
TGGTCCCACAAACAATTGCTGAAAGTTGTATCCCATACGCCCATCGAGGTGGAGCATTTGGCCGAGGCGCAGGACGCCGG
GGGATGGGTCGACGTCACCCGCAAGGATATCCCCGACGGGCCGCTATTCTCGTGGTGGTCCTACCGCTCGCCGGATTGGG
ACAATGCCGACAAGGGGCGTCGTCTCGATCATGTGTGGGCCACGCCCGATATCTCCAATGCTGCGCATTCCTCCCGCATT
GCCCGCCATGTGCGCGGGTGGGAAAAGCCGTCCGATCACGCCCCGGTTTTTGCGACTTTCGACCTCTGA

Upstream 100 bases:

>100_bases
CGGGGGAGTCTGCGGCCTTTAGGCCGTGGACGGGGGCTGGCCCCCTAGCCACGCCCGCCCAAATCACTATCGTGATCGAA
ACTCGAAACCGGAGCCCGAC

Downstream 100 bases:

>100_bases
CCCTTGGCGAACGGCCCCGCGCCGCGCATATAGAGCGCAACACATGCAAATGACGGAGCTTGCACCATGCTCGAACTCGG
CCAAACCCCGAACGCGGCCC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MPFTLATWNINSVRLRQPIVSQLMTEEAPDILCLQECKSPVDKMPSDVFAALGYTHVVARGQKGYNGVAIYSKLPMEEAG
SHEFAGLDHARHIAGRLENGVTIHNFYVPAGGDVPDRDVNIKFGQKLDYLTDMRDWAKTDTPQKSILLGDLNIAPREDDV
WSHKQLLKVVSHTPIEVEHLAEAQDAGGWVDVTRKDIPDGPLFSWWSYRSPDWDNADKGRRLDHVWATPDISNAAHSSRI
ARHVRGWEKPSDHAPVFATFDL

Sequences:

>Translated_262_residues
MPFTLATWNINSVRLRQPIVSQLMTEEAPDILCLQECKSPVDKMPSDVFAALGYTHVVARGQKGYNGVAIYSKLPMEEAG
SHEFAGLDHARHIAGRLENGVTIHNFYVPAGGDVPDRDVNIKFGQKLDYLTDMRDWAKTDTPQKSILLGDLNIAPREDDV
WSHKQLLKVVSHTPIEVEHLAEAQDAGGWVDVTRKDIPDGPLFSWWSYRSPDWDNADKGRRLDHVWATPDISNAAHSSRI
ARHVRGWEKPSDHAPVFATFDL
>Mature_261_residues
PFTLATWNINSVRLRQPIVSQLMTEEAPDILCLQECKSPVDKMPSDVFAALGYTHVVARGQKGYNGVAIYSKLPMEEAGS
HEFAGLDHARHIAGRLENGVTIHNFYVPAGGDVPDRDVNIKFGQKLDYLTDMRDWAKTDTPQKSILLGDLNIAPREDDVW
SHKQLLKVVSHTPIEVEHLAEAQDAGGWVDVTRKDIPDGPLFSWWSYRSPDWDNADKGRRLDHVWATPDISNAAHSSRIA
RHVRGWEKPSDHAPVFATFDL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Escherichia coli, GI1788046, Length=272, Percent_Identity=32.7205882352941, Blast_Score=108, Evalue=4e-25,
Organism=Drosophila melanogaster, GI221330655, Length=267, Percent_Identity=28.4644194756554, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI17136678, Length=267, Percent_Identity=28.4644194756554, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29425; Mature: 29294

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFTLATWNINSVRLRQPIVSQLMTEEAPDILCLQECKSPVDKMPSDVFAALGYTHVVAR
CCEEEEEECCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCHHHHHHHHHHHHHHC
GQKGYNGVAIYSKLPMEEAGSHEFAGLDHARHIAGRLENGVTIHNFYVPAGGDVPDRDVN
CCCCCCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEE
IKFGQKLDYLTDMRDWAKTDTPQKSILLGDLNIAPREDDVWSHKQLLKVVSHTPIEVEHL
EECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHH
AEAQDAGGWVDVTRKDIPDGPLFSWWSYRSPDWDNADKGRRLDHVWATPDISNAAHSSRI
HHHHHCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHH
ARHVRGWEKPSDHAPVFATFDL
HHHHHCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
PFTLATWNINSVRLRQPIVSQLMTEEAPDILCLQECKSPVDKMPSDVFAALGYTHVVAR
CEEEEEECCCCEEHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCHHHHHHHHHHHHHHC
GQKGYNGVAIYSKLPMEEAGSHEFAGLDHARHIAGRLENGVTIHNFYVPAGGDVPDRDVN
CCCCCCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEE
IKFGQKLDYLTDMRDWAKTDTPQKSILLGDLNIAPREDDVWSHKQLLKVVSHTPIEVEHL
EECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHH
AEAQDAGGWVDVTRKDIPDGPLFSWWSYRSPDWDNADKGRRLDHVWATPDISNAAHSSRI
HHHHHCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHH
ARHVRGWEKPSDHAPVFATFDL
HHHHHCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]