Click here to switch to the map view.

The map label for this gene is rnhB

Identifier: 89052882

GI number: 89052882

Start: 394195

End: 394794

Strand: Direct

Name: rnhB

Synonym: Jann_0391

Alternate gene names: 89052882

Gene position: 394195-394794 (Clockwise)

Preceding gene: 89052881

Following gene: 89052883

Centisome position: 9.13

GC content: 63.83

Gene sequence:

>600_bases
ATGGGACCTGATTTTGAGATTGAGCGCGAGCTGGGTGGCCTTGTCGCGGGCGTGGATGAAGTGGGGCGCGGGCCGTGGGC
CGGTCCCGTAACCGCCTGCGCCGTGGTTCTGGACCCCATGCAGGTCCCCGACGGCCTGAACGATTCCAAGAAGCTGTCAG
AGGCGCGGCGCGATGCCTTGGCGATGCAGATCCTGCGGGTCGCCGATGTCTCGCTGGGCTGGGCCAGCGTCGAGGAGATC
GACGCTCTCAACATCCGCCAGGCCACATTTCTTGCCATGCGCCGCGCGATGGACGGTCTGACCACCCCACCGACCCACGC
GCTGATCGACGGAAACGCCATCCCGCCGGGCTTGTCCTGTCCGGCCACCTGCGTCGTGAAGGGGGACGGGCGGTCTGTCT
CCATCGCGGCGGCGTCAATTGTGGCCAAAGTGCGGCGCGACGCCCTGATGAAAGAGCTTGCTGTGATGCATCCCGGTTAC
GGTTGGGAGACAAACATGGGCTATGGCACCGCCAAACACGCCGCTGGTCTACATCATCTAGGGGTGACCCAATATCATCG
GCGCAGCTTTGCGCCCATTGCCAAGATCTTGTGTGGATAA

Upstream 100 bases:

>100_bases
CCCGGCGGACCCCCTGCGCGGCAAAACCGACCGAGCAGTCAGGGCGGGGCGGCGTGACCATCTGGTTGCGCCGCCTTTGT
TGTTGGGCCATGGCGATGGC

Downstream 100 bases:

>100_bases
AATCGTACGGATTCGATTCGAAGCAAATTGACCGGGATTCGAAGTTCGTGGAATCTGACCTCAATAACAATGAGCGCCCA
AAGAGGCGCCTGTGCGAGGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MGPDFEIERELGGLVAGVDEVGRGPWAGPVTACAVVLDPMQVPDGLNDSKKLSEARRDALAMQILRVADVSLGWASVEEI
DALNIRQATFLAMRRAMDGLTTPPTHALIDGNAIPPGLSCPATCVVKGDGRSVSIAAASIVAKVRRDALMKELAVMHPGY
GWETNMGYGTAKHAAGLHHLGVTQYHRRSFAPIAKILCG

Sequences:

>Translated_199_residues
MGPDFEIERELGGLVAGVDEVGRGPWAGPVTACAVVLDPMQVPDGLNDSKKLSEARRDALAMQILRVADVSLGWASVEEI
DALNIRQATFLAMRRAMDGLTTPPTHALIDGNAIPPGLSCPATCVVKGDGRSVSIAAASIVAKVRRDALMKELAVMHPGY
GWETNMGYGTAKHAAGLHHLGVTQYHRRSFAPIAKILCG
>Mature_198_residues
GPDFEIERELGGLVAGVDEVGRGPWAGPVTACAVVLDPMQVPDGLNDSKKLSEARRDALAMQILRVADVSLGWASVEEID
ALNIRQATFLAMRRAMDGLTTPPTHALIDGNAIPPGLSCPATCVVKGDGRSVSIAAASIVAKVRRDALMKELAVMHPGYG
WETNMGYGTAKHAAGLHHLGVTQYHRRSFAPIAKILCG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=185, Percent_Identity=54.5945945945946, Blast_Score=171, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_JANSC (Q28VF4)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508333.1
- ProteinModelPortal:   Q28VF4
- SMR:   Q28VF4
- STRING:   Q28VF4
- GeneID:   3932832
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0391
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q28VF4
- BioCyc:   JSP290400:JANN_0391-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21063; Mature: 20931

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPDFEIERELGGLVAGVDEVGRGPWAGPVTACAVVLDPMQVPDGLNDSKKLSEARRDAL
CCCCCCHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHH
AMQILRVADVSLGWASVEEIDALNIRQATFLAMRRAMDGLTTPPTHALIDGNAIPPGLSC
HHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCC
PATCVVKGDGRSVSIAAASIVAKVRRDALMKELAVMHPGYGWETNMGYGTAKHAAGLHHL
CEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHH
GVTQYHRRSFAPIAKILCG
CHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GPDFEIERELGGLVAGVDEVGRGPWAGPVTACAVVLDPMQVPDGLNDSKKLSEARRDAL
CCCCCHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHH
AMQILRVADVSLGWASVEEIDALNIRQATFLAMRRAMDGLTTPPTHALIDGNAIPPGLSC
HHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCC
PATCVVKGDGRSVSIAAASIVAKVRRDALMKELAVMHPGYGWETNMGYGTAKHAAGLHHL
CEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHH
GVTQYHRRSFAPIAKILCG
CHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA