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The map label for this gene is nudG [H]

Identifier: 89052783

GI number: 89052783

Start: 300162

End: 300548

Strand: Direct

Name: nudG [H]

Synonym: Jann_0292

Alternate gene names: 89052783

Gene position: 300162-300548 (Clockwise)

Preceding gene: 89052782

Following gene: 89052786

Centisome position: 6.95

GC content: 61.76

Gene sequence:

>387_bases
TTGGTGTCCGCCGTCGCCCTGATCGACGCAGAGGGGCGTTTGTTGCTCGCGCAGCGGCCCGAAGGGAAGTCCATGGCCGG
CCTGTGGGAATTTCCCGGCGGCAAGGTTGAAGACGGTGAAACGCCGGAACAGGCATTGATCCGGGAGTTGCAGGAAGAGC
TTGGCATCGACACTTGGGAAAGCTGCCTCGCGCCGCTGACCTTTGCCAGCCACGCCTACGAGACATTCCACCTGCTTATG
CCGCTTTTTGCCTGCCGCAAATGGAACGGCATTCCGCAATCGCGGGAGGGGCAGACGCTCAAATGGGTGCGCGCGGCTGA
TCTGCGCGATTACCCCATGCCTCCTGCCGATATTCCCCTGATCCCGATCCTTCGCGACTGGCTGTAA

Upstream 100 bases:

>100_bases
TCGATCTGGGCCTCGGTTCCGGCGCGGCGACGGTCTGGACCTGTGACCTGACCCACGGATATATCCAGATCAACGCGGAT
TATCGGTCGTGAGCATTCTT

Downstream 100 bases:

>100_bases
CCGCAAGTTGTATACATGTGCGCAAAAAATGTGCAGAAAAATGCGCCAATCGGTCATTTTCTGAGTGCTTTGTTAATCTT
TCTCAGTAGACTGAATTAAC

Product: mutator mutT protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MVSAVALIDAEGRLLLAQRPEGKSMAGLWEFPGGKVEDGETPEQALIRELQEELGIDTWESCLAPLTFASHAYETFHLLM
PLFACRKWNGIPQSREGQTLKWVRAADLRDYPMPPADIPLIPILRDWL

Sequences:

>Translated_128_residues
MVSAVALIDAEGRLLLAQRPEGKSMAGLWEFPGGKVEDGETPEQALIRELQEELGIDTWESCLAPLTFASHAYETFHLLM
PLFACRKWNGIPQSREGQTLKWVRAADLRDYPMPPADIPLIPILRDWL
>Mature_128_residues
MVSAVALIDAEGRLLLAQRPEGKSMAGLWEFPGGKVEDGETPEQALIRELQEELGIDTWESCLAPLTFASHAYETFHLLM
PLFACRKWNGIPQSREGQTLKWVRAADLRDYPMPPADIPLIPILRDWL

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=124, Percent_Identity=43.5483870967742, Blast_Score=89, Evalue=9e-20,
Organism=Escherichia coli, GI1786288, Length=125, Percent_Identity=38.4, Blast_Score=77, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.-

Molecular weight: Translated: 14414; Mature: 14414

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSAVALIDAEGRLLLAQRPEGKSMAGLWEFPGGKVEDGETPEQALIRELQEELGIDTWE
CCCEEEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHH
SCLAPLTFASHAYETFHLLMPLFACRKWNGIPQSREGQTLKWVRAADLRDYPMPPADIPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHCCCCCCCCCCCCCCCCH
IPILRDWL
HHHHHHCC
>Mature Secondary Structure
MVSAVALIDAEGRLLLAQRPEGKSMAGLWEFPGGKVEDGETPEQALIRELQEELGIDTWE
CCCEEEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHH
SCLAPLTFASHAYETFHLLMPLFACRKWNGIPQSREGQTLKWVRAADLRDYPMPPADIPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHCCCCCCCCCCCCCCCCH
IPILRDWL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]