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The map label for this gene is gltD [H]

Identifier: 89052739

GI number: 89052739

Start: 244681

End: 246117

Strand: Direct

Name: gltD [H]

Synonym: Jann_0248

Alternate gene names: 89052739

Gene position: 244681-246117 (Clockwise)

Preceding gene: 89052737

Following gene: 89052740

Centisome position: 5.67

GC content: 59.22

Gene sequence:

>1437_bases
ATGGCGAAGCAACCCATGTTGAAGTTTGTAACCGTTGGCAAGGAAATGCCTCAAAAGCGGGAAGCGGACCAGCGGAAAGA
GGATTTCGCCGAGATCTACGGCGAGTATGTGACCGCCAAGGCAGAGGAGCAGGCCGGGCGTTGCAGCCAGTGTGGAGTGC
CCTATTGTCAATCTCACTGCCCGCTGCATAACAATATACCCGATTGGTTAAAATTAACTGCAGAAGGGCGGCTGCGCGAG
GCCTATGACCTCAGCCAAGCCACCAACACCTTCCCTGAGATCTGTGGCCGCATCTGCCCGCAGGACCGTTTGTGCGAGGG
AAATTGCGTGATCGAGCAGTCGGGCCATGGCACTGTTACCATCGGCTCGGTCGAGAAATATATCACCGATACCGCCTGGG
ATAACGGGTGGGTCATGCCCATTGCGCCGCATACAGAACGCCCTGAATCCGTTGGGATAATCGGTGCCGGCCCCGGTGGC
TTGGCCGCCGCAGATATGCTGCGGCGCGCGGGTCTCCAGGTCACTGTTTACGATCGCTATGATCGCGCGGGTGGGCTGAT
GGTCTATGGCATTCCTGGCTTCAAGCTGGAGAAAGACGTTGTGATGCGGCGCAACAAACAGCTGGAAGATGGTGGCGTGG
AGTTCGTGTTGAACTGCAATGTCGGCGAGGACATTTCGTTAACCGACATCCGCGAAAAGCACGATTTCGTCGTGATTGCC
ACGGGTGTCTACAAGTCGCGTGAGCTGGACGGCCCCGGATCGGGCAGCGACGGGATTATCCGCGCGATTGATTATCTAAC
CGCCTCTAATCGCAAGTCTTTTGGCGATGACGTGCCGGAATTTGACAGCGGCGAGTTGGACGCCAAGGGAAAGCGGATTG
TGGTTATTGGTGGCGGCGACACGGCGATGGACTGTGTCCGCACGGCGACGCGGCAGGGGGCGACCTCGGTCAAATGCCTC
TACCGTCGCGACGAGGTCAATATGCCCGGCTCCATGCGTGAAGTGCAGAACGCCAAGGAAGAAGGTGTTGAATTCGTTTG
GCTTTCCGCACCCAAGGGGTTCAGCGACGCCAAGGGTGCCGTTGATGGTGTCATGGTCCAGAAAATGCGCCTCGGCGCGC
CCGACGCAACGGGGCGTCAAAGCCCGGAAGTCATTGAAAACGCAGATTATGTTGAGGGCGCGGACATGGTGATCAAGGCG
CTGGGCTTCTCCCCCGAGGATTTGCCGATGCTGTGGGACACGCCCGAGTTGGAGATCACGCGCTGGGGCACTGTGAAGGC
CAACCGCACCCATGAAACCGCCCTGCCCAATGTTTTCGCGGTGGGTGATATCGTGCGGGGTGCCAGCCTGGTCGTCTGGG
CGATCCGTGATGGCCGTGAGGCAGCCGAGGCGATCCTTGAGCGGGTTTACGCAACCGGTACGGTGGCCGCGGAGTGA

Upstream 100 bases:

>100_bases
ATCGCAGATCGCTTAATACCTCAAAATAGGACAGTAATTCTGTCTTTTTATCCCCGGAAACCCCCGGTATATGGGCAGCT
CACTCGGCGGAGGAAGCGTG

Downstream 100 bases:

>100_bases
GTGTCGGTTAACGCGAGATCTGAACCCGCTTGGCCCGACCGGCCTTTGCAAGAACCTGTAATTATCCTGTAGTTTTTCTG
TAACTACACCCGTCTCTCAG

Product: putative oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MAKQPMLKFVTVGKEMPQKREADQRKEDFAEIYGEYVTAKAEEQAGRCSQCGVPYCQSHCPLHNNIPDWLKLTAEGRLRE
AYDLSQATNTFPEICGRICPQDRLCEGNCVIEQSGHGTVTIGSVEKYITDTAWDNGWVMPIAPHTERPESVGIIGAGPGG
LAAADMLRRAGLQVTVYDRYDRAGGLMVYGIPGFKLEKDVVMRRNKQLEDGGVEFVLNCNVGEDISLTDIREKHDFVVIA
TGVYKSRELDGPGSGSDGIIRAIDYLTASNRKSFGDDVPEFDSGELDAKGKRIVVIGGGDTAMDCVRTATRQGATSVKCL
YRRDEVNMPGSMREVQNAKEEGVEFVWLSAPKGFSDAKGAVDGVMVQKMRLGAPDATGRQSPEVIENADYVEGADMVIKA
LGFSPEDLPMLWDTPELEITRWGTVKANRTHETALPNVFAVGDIVRGASLVVWAIRDGREAAEAILERVYATGTVAAE

Sequences:

>Translated_478_residues
MAKQPMLKFVTVGKEMPQKREADQRKEDFAEIYGEYVTAKAEEQAGRCSQCGVPYCQSHCPLHNNIPDWLKLTAEGRLRE
AYDLSQATNTFPEICGRICPQDRLCEGNCVIEQSGHGTVTIGSVEKYITDTAWDNGWVMPIAPHTERPESVGIIGAGPGG
LAAADMLRRAGLQVTVYDRYDRAGGLMVYGIPGFKLEKDVVMRRNKQLEDGGVEFVLNCNVGEDISLTDIREKHDFVVIA
TGVYKSRELDGPGSGSDGIIRAIDYLTASNRKSFGDDVPEFDSGELDAKGKRIVVIGGGDTAMDCVRTATRQGATSVKCL
YRRDEVNMPGSMREVQNAKEEGVEFVWLSAPKGFSDAKGAVDGVMVQKMRLGAPDATGRQSPEVIENADYVEGADMVIKA
LGFSPEDLPMLWDTPELEITRWGTVKANRTHETALPNVFAVGDIVRGASLVVWAIRDGREAAEAILERVYATGTVAAE
>Mature_477_residues
AKQPMLKFVTVGKEMPQKREADQRKEDFAEIYGEYVTAKAEEQAGRCSQCGVPYCQSHCPLHNNIPDWLKLTAEGRLREA
YDLSQATNTFPEICGRICPQDRLCEGNCVIEQSGHGTVTIGSVEKYITDTAWDNGWVMPIAPHTERPESVGIIGAGPGGL
AAADMLRRAGLQVTVYDRYDRAGGLMVYGIPGFKLEKDVVMRRNKQLEDGGVEFVLNCNVGEDISLTDIREKHDFVVIAT
GVYKSRELDGPGSGSDGIIRAIDYLTASNRKSFGDDVPEFDSGELDAKGKRIVVIGGGDTAMDCVRTATRQGATSVKCLY
RRDEVNMPGSMREVQNAKEEGVEFVWLSAPKGFSDAKGAVDGVMVQKMRLGAPDATGRQSPEVIENADYVEGADMVIKAL
GFSPEDLPMLWDTPELEITRWGTVKANRTHETALPNVFAVGDIVRGASLVVWAIRDGREAAEAILERVYATGTVAAE

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=457, Percent_Identity=26.4770240700219, Blast_Score=138, Evalue=9e-33,
Organism=Escherichia coli, GI1789606, Length=468, Percent_Identity=44.8717948717949, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1788811, Length=458, Percent_Identity=44.5414847161572, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI87082180, Length=455, Percent_Identity=43.0769230769231, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1789243, Length=425, Percent_Identity=31.5294117647059, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788468, Length=451, Percent_Identity=28.159645232816, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17570289, Length=497, Percent_Identity=34.20523138833, Blast_Score=254, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI71984108, Length=450, Percent_Identity=27.3333333333333, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17543860, Length=164, Percent_Identity=29.8780487804878, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=485, Percent_Identity=34.8453608247423, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI28574881, Length=483, Percent_Identity=37.4741200828157, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24665539, Length=483, Percent_Identity=37.4741200828157, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24665547, Length=483, Percent_Identity=37.4741200828157, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24665543, Length=483, Percent_Identity=37.4741200828157, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24640763, Length=466, Percent_Identity=25.3218884120172, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI18858217, Length=466, Percent_Identity=25.3218884120172, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52165; Mature: 52033

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQPMLKFVTVGKEMPQKREADQRKEDFAEIYGEYVTAKAEEQAGRCSQCGVPYCQSHC
CCCCCHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHCCCCHHHCCC
PLHNNIPDWLKLTAEGRLREAYDLSQATNTFPEICGRICPQDRLCEGNCVIEQSGHGTVT
CCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEE
IGSVEKYITDTAWDNGWVMPIAPHTERPESVGIIGAGPGGLAAADMLRRAGLQVTVYDRY
ECCHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEECC
DRAGGLMVYGIPGFKLEKDVVMRRNKQLEDGGVEFVLNCNVGEDISLTDIREKHDFVVIA
CCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCEEEEE
TGVYKSRELDGPGSGSDGIIRAIDYLTASNRKSFGDDVPEFDSGELDAKGKRIVVIGGGD
ECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
TAMDCVRTATRQGATSVKCLYRRDEVNMPGSMREVQNAKEEGVEFVWLSAPKGFSDAKGA
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
VDGVMVQKMRLGAPDATGRQSPEVIENADYVEGADMVIKALGFSPEDLPMLWDTPELEIT
HHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCEEEE
RWGTVKANRTHETALPNVFAVGDIVRGASLVVWAIRDGREAAEAILERVYATGTVAAE
EECEEECCCCCHHHCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AKQPMLKFVTVGKEMPQKREADQRKEDFAEIYGEYVTAKAEEQAGRCSQCGVPYCQSHC
CCCCHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHCCCCHHHCCC
PLHNNIPDWLKLTAEGRLREAYDLSQATNTFPEICGRICPQDRLCEGNCVIEQSGHGTVT
CCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEE
IGSVEKYITDTAWDNGWVMPIAPHTERPESVGIIGAGPGGLAAADMLRRAGLQVTVYDRY
ECCHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEECC
DRAGGLMVYGIPGFKLEKDVVMRRNKQLEDGGVEFVLNCNVGEDISLTDIREKHDFVVIA
CCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCEEEEE
TGVYKSRELDGPGSGSDGIIRAIDYLTASNRKSFGDDVPEFDSGELDAKGKRIVVIGGGD
ECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
TAMDCVRTATRQGATSVKCLYRRDEVNMPGSMREVQNAKEEGVEFVWLSAPKGFSDAKGA
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
VDGVMVQKMRLGAPDATGRQSPEVIENADYVEGADMVIKALGFSPEDLPMLWDTPELEIT
HHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCEEEE
RWGTVKANRTHETALPNVFAVGDIVRGASLVVWAIRDGREAAEAILERVYATGTVAAE
EECEEECCCCCHHHCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8428988; 2198943 [H]