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The map label for this gene is alkB [H]

Identifier: 89052704

GI number: 89052704

Start: 208414

End: 209064

Strand: Direct

Name: alkB [H]

Synonym: Jann_0213

Alternate gene names: 89052704

Gene position: 208414-209064 (Clockwise)

Preceding gene: 89052701

Following gene: 89052706

Centisome position: 4.83

GC content: 61.29

Gene sequence:

>651_bases
GTGATTTGGGGCTGCAAGCGGCAGCACGCGATGGAATCCGAGGAATTTGCATTGAGTGTCGATGTCGGTGGGATTGTGGT
GCATCCCGAACACCTGGACGGGCCGGATCAAGCGGAACTGGTGGAGCAGGTGCGGCGTGTCGTGCGGTCCGCGCCACTAT
ACCGGCCCGAGACGCGGACGGGACGCAAGATGTCCGTGCGCATGACGTCTGCGGGGACGTATGGATGGATCTCGGACCGG
AGGGGCTATCGCTATGACCGGTGTCATCCGGACGGGCAGGATTGGCCGCCGATCCCGCCCATGGCGCTGGAGATTTGGCG
GGCGGTGTCGGGTGTGGCACAGGATCCGCAAAGTTGTCTGATCAACTATTATGATGCGGGTGCAAAGATGGGGATGCATC
AGGACCGGGATGAAGGGGATTTCGACATGCCGGTTGTCTCTGTGTCGCTGGGGGATGAGGCGCTGTTTCGTGTGGGCGGT
CCGAAGCGTGGCGGCAAAACGCAATCGGTCTGGCTGAAATCTGGCGATGTGGCTGTAATGGGCGGAGAGGCCCGTCTGAA
CTTCCACGGCATAGACCGCATTCGCGCAGGCTCCTCTACGCTTTTGCCCAATGGCGGGCGGATCAATCTGACGATGCGGG
TCGTGACATAG

Upstream 100 bases:

>100_bases
TGGCGGGAGCGGGTCCGAAGTTACGGCCCCCGTGCCGCCGTTATAGTGACTGTAACGGACATTGATGTGCCCGCATGAGT
TCGGAGGGTATATAAGAAGC

Downstream 100 bases:

>100_bases
GCCCTAGCGCTTTGCGTCCCAACTGGAGGAGGCATGTTTGCGGGTATATGCCTCAAACATCATGCCGAACAAAATGGCGC
CGAGCGTGAAGTAGAACGGA

Product: DNA-N1-methyladenine dioxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MIWGCKRQHAMESEEFALSVDVGGIVVHPEHLDGPDQAELVEQVRRVVRSAPLYRPETRTGRKMSVRMTSAGTYGWISDR
RGYRYDRCHPDGQDWPPIPPMALEIWRAVSGVAQDPQSCLINYYDAGAKMGMHQDRDEGDFDMPVVSVSLGDEALFRVGG
PKRGGKTQSVWLKSGDVAVMGGEARLNFHGIDRIRAGSSTLLPNGGRINLTMRVVT

Sequences:

>Translated_216_residues
MIWGCKRQHAMESEEFALSVDVGGIVVHPEHLDGPDQAELVEQVRRVVRSAPLYRPETRTGRKMSVRMTSAGTYGWISDR
RGYRYDRCHPDGQDWPPIPPMALEIWRAVSGVAQDPQSCLINYYDAGAKMGMHQDRDEGDFDMPVVSVSLGDEALFRVGG
PKRGGKTQSVWLKSGDVAVMGGEARLNFHGIDRIRAGSSTLLPNGGRINLTMRVVT
>Mature_216_residues
MIWGCKRQHAMESEEFALSVDVGGIVVHPEHLDGPDQAELVEQVRRVVRSAPLYRPETRTGRKMSVRMTSAGTYGWISDR
RGYRYDRCHPDGQDWPPIPPMALEIWRAVSGVAQDPQSCLINYYDAGAKMGMHQDRDEGDFDMPVVSVSLGDEALFRVGG
PKRGGKTQSVWLKSGDVAVMGGEARLNFHGIDRIRAGSSTLLPNGGRINLTMRVVT

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=170, Percent_Identity=26.4705882352941, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1788541, Length=207, Percent_Identity=35.7487922705314, Blast_Score=125, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45555401, Length=88, Percent_Identity=37.5, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23938; Mature: 23938

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWGCKRQHAMESEEFALSVDVGGIVVHPEHLDGPDQAELVEQVRRVVRSAPLYRPETRT
CCCCCCHHHCCCCCCEEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
GRKMSVRMTSAGTYGWISDRRGYRYDRCHPDGQDWPPIPPMALEIWRAVSGVAQDPQSCL
CCEEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHE
INYYDAGAKMGMHQDRDEGDFDMPVVSVSLGDEALFRVGGPKRGGKTQSVWLKSGDVAVM
EEHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCEEEEEECCCEEEE
GGEARLNFHGIDRIRAGSSTLLPNGGRINLTMRVVT
CCCEEEEECCCCEEECCCCEECCCCCEEEEEEEEEC
>Mature Secondary Structure
MIWGCKRQHAMESEEFALSVDVGGIVVHPEHLDGPDQAELVEQVRRVVRSAPLYRPETRT
CCCCCCHHHCCCCCCEEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
GRKMSVRMTSAGTYGWISDRRGYRYDRCHPDGQDWPPIPPMALEIWRAVSGVAQDPQSCL
CCEEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHE
INYYDAGAKMGMHQDRDEGDFDMPVVSVSLGDEALFRVGGPKRGGKTQSVWLKSGDVAVM
EEHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCEEEEEECCCEEEE
GGEARLNFHGIDRIRAGSSTLLPNGGRINLTMRVVT
CCCEEEEECCCCEEECCCCEECCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9150207 [H]