Click here to switch to the map view.

The map label for this gene is argG

Identifier: 89052558

GI number: 89052558

Start: 61301

End: 62524

Strand: Reverse

Name: argG

Synonym: Jann_0067

Alternate gene names: 89052558

Gene position: 62524-61301 (Counterclockwise)

Preceding gene: 89052561

Following gene: 89052552

Centisome position: 1.45

GC content: 60.87

Gene sequence:

>1224_bases
ATGTCAGCGCCCAAAAAGGTTGTGCTCGCCTATTCCGGCGGCCTTGATACCTCGATCATCCTGAAATGGTTGCAGACGGA
ATATGGCTGCGAGGTGGTGACCTTCACCGCCGATCTGGGCCAGGGGGAAGAGTTGGAGCCTGCCCGCAAGAAGGCCGAGA
TGATGGGGGCCGCGGGCATCTATGTGGAGGACCTGCGCGAGGAATTCGTGCGTGATTTCGTCTTCCCGATGTTCCGCGCC
AACGCGCTGTATGAGGGGTTGTACCTTCTGGGCACCTCCATCGCGCGCCCTCTGATCTCCAAGCGGCTGGTGGAGATTGC
CGAGGAAACCGGGGCCGATGCCGTGTCCCATGGTGCGACGGGCAAGGGCAATGATCAGGTCCGGTTTGAACTTTGCGCCT
ATTCGCTCAACCCTGAAATCGAGGTGATCGCGCCCTGGCGAAAGTGGGAGCTGGGAAGCCGGACAAAGCTGATCGAGTTC
GCCGAAAAGCATCAGATCCCGATTGCCAAGGACAAGCGTGGGGAGGCCCCGTTCAGCGTCGACGCAAACCTTCTGCACAC
CTCATCCGAGGGCAAGATGCTGGAGGATCCGGCCGAGGAAGCGCCTGACCATATCCTCCAGCGCATCACCCGCCCGGAGG
ACGCGCCCGACACGCCCGAGATGGTAGAGATCACCTTCGAGAAGGGCGACGCCGTCGCGATCAATGGCGAGCCGATGAGC
CCGGCCACGATCCTGACCGAGCTGAACCGCCTGGGCGGCAAGCACGGCATCGGCATCCTTGATCTGGTGGAAAACCGCTT
CGTCGGCATGAAATCCCGCGGCATCTACGAGACGCCCGGCGGTACGGTCCTGCTGGAAGCGCACCGGGGGATCGAGCAGA
TCACCCTCGATGGGGCGTCCGGCCATCTCAAGGACAGCATCATGCCGCGCTATGCAGAGCTGATTTACAACGGTTTCTGG
TTCTCGCCCGAGCGGGAAATGCTGCAAGCGCTGATCGACAAAAGCCAAGAGCATGTCACCGGCACCGTTCGCCTGAAACT
CTATAAAGGGGCCGCGCGCTGCGTTGCCCGCTGGTCTGACCATTCCCTCTACTCAGAGGCCCACGTCACGTTTGAGGAAG
ACATGGGAGCCTATGACCAATCCGATGCCCAGGGCTTCATTCAGCTCAACGCGCTGCGCCTTAAGTTGCTGTCCGCCCGC
AATCGCAAGTTTAACGGAAGTTAG

Upstream 100 bases:

>100_bases
AGTTGATAACCGTAATCCATGGGGCGCTCCACTTGCTTGCTTGCCCCTTGAAAACCCGGTATCGCCGCTGCGGTCAATCG
CTACTTACGGAGTGTTCCCC

Downstream 100 bases:

>100_bases
TGCGCCCCTTTTAGGGGCTCACTATTTCCTGGCTACGCTTGTTGCTGTTCTCTTACCGGGGCAAGTGTTGAACTTTTCAC
AAGATTGATTGCTGAAAGGC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSAPKKVVLAYSGGLDTSIILKWLQTEYGCEVVTFTADLGQGEELEPARKKAEMMGAAGIYVEDLREEFVRDFVFPMFRA
NALYEGLYLLGTSIARPLISKRLVEIAEETGADAVSHGATGKGNDQVRFELCAYSLNPEIEVIAPWRKWELGSRTKLIEF
AEKHQIPIAKDKRGEAPFSVDANLLHTSSEGKMLEDPAEEAPDHILQRITRPEDAPDTPEMVEITFEKGDAVAINGEPMS
PATILTELNRLGGKHGIGILDLVENRFVGMKSRGIYETPGGTVLLEAHRGIEQITLDGASGHLKDSIMPRYAELIYNGFW
FSPEREMLQALIDKSQEHVTGTVRLKLYKGAARCVARWSDHSLYSEAHVTFEEDMGAYDQSDAQGFIQLNALRLKLLSAR
NRKFNGS

Sequences:

>Translated_407_residues
MSAPKKVVLAYSGGLDTSIILKWLQTEYGCEVVTFTADLGQGEELEPARKKAEMMGAAGIYVEDLREEFVRDFVFPMFRA
NALYEGLYLLGTSIARPLISKRLVEIAEETGADAVSHGATGKGNDQVRFELCAYSLNPEIEVIAPWRKWELGSRTKLIEF
AEKHQIPIAKDKRGEAPFSVDANLLHTSSEGKMLEDPAEEAPDHILQRITRPEDAPDTPEMVEITFEKGDAVAINGEPMS
PATILTELNRLGGKHGIGILDLVENRFVGMKSRGIYETPGGTVLLEAHRGIEQITLDGASGHLKDSIMPRYAELIYNGFW
FSPEREMLQALIDKSQEHVTGTVRLKLYKGAARCVARWSDHSLYSEAHVTFEEDMGAYDQSDAQGFIQLNALRLKLLSAR
NRKFNGS
>Mature_406_residues
SAPKKVVLAYSGGLDTSIILKWLQTEYGCEVVTFTADLGQGEELEPARKKAEMMGAAGIYVEDLREEFVRDFVFPMFRAN
ALYEGLYLLGTSIARPLISKRLVEIAEETGADAVSHGATGKGNDQVRFELCAYSLNPEIEVIAPWRKWELGSRTKLIEFA
EKHQIPIAKDKRGEAPFSVDANLLHTSSEGKMLEDPAEEAPDHILQRITRPEDAPDTPEMVEITFEKGDAVAINGEPMSP
ATILTELNRLGGKHGIGILDLVENRFVGMKSRGIYETPGGTVLLEAHRGIEQITLDGASGHLKDSIMPRYAELIYNGFWF
SPEREMLQALIDKSQEHVTGTVRLKLYKGAARCVARWSDHSLYSEAHVTFEEDMGAYDQSDAQGFIQLNALRLKLLSARN
RKFNGS

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=404, Percent_Identity=48.5148514851485, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI16950633, Length=404, Percent_Identity=48.5148514851485, Blast_Score=366, Evalue=1e-101,
Organism=Escherichia coli, GI1789563, Length=423, Percent_Identity=30.2600472813239, Blast_Score=143, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324514, Length=405, Percent_Identity=42.2222222222222, Blast_Score=318, Evalue=1e-87,
Organism=Drosophila melanogaster, GI21358151, Length=407, Percent_Identity=44.4717444717445, Blast_Score=332, Evalue=2e-91,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_JANSC (Q28WC8)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508009.1
- ProteinModelPortal:   Q28WC8
- SMR:   Q28WC8
- STRING:   Q28WC8
- GeneID:   3932503
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0067
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- PhylomeDB:   Q28WC8
- ProtClustDB:   PRK00509
- BioCyc:   JSP290400:JANN_0067-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45204; Mature: 45073

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 37-37 BINDING 88-88 BINDING 93-93 BINDING 118-118 BINDING 120-120 BINDING 124-124 BINDING 124-124 BINDING 125-125 BINDING 128-128 BINDING 179-179 BINDING 188-188 BINDING 264-264 BINDING 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPKKVVLAYSGGLDTSIILKWLQTEYGCEVVTFTADLGQGEELEPARKKAEMMGAAGI
CCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCH
YVEDLREEFVRDFVFPMFRANALYEGLYLLGTSIARPLISKRLVEIAEETGADAVSHGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
GKGNDQVRFELCAYSLNPEIEVIAPWRKWELGSRTKLIEFAEKHQIPIAKDKRGEAPFSV
CCCCCCEEEEEEEEECCCCEEEEECCHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
DANLLHTSSEGKMLEDPAEEAPDHILQRITRPEDAPDTPEMVEITFEKGDAVAINGEPMS
CCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEECCCCCC
PATILTELNRLGGKHGIGILDLVENRFVGMKSRGIYETPGGTVLLEAHRGIEQITLDGAS
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCEEEEECCCCCEEEEECCCC
GHLKDSIMPRYAELIYNGFWFSPEREMLQALIDKSQEHVTGTVRLKLYKGAARCVARWSD
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHEEEEEEEEEECCHHHHHHHCCC
HSLYSEAHVTFEEDMGAYDQSDAQGFIQLNALRLKLLSARNRKFNGS
CCHHHHHCEEEHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAPKKVVLAYSGGLDTSIILKWLQTEYGCEVVTFTADLGQGEELEPARKKAEMMGAAGI
CCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCH
YVEDLREEFVRDFVFPMFRANALYEGLYLLGTSIARPLISKRLVEIAEETGADAVSHGAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
GKGNDQVRFELCAYSLNPEIEVIAPWRKWELGSRTKLIEFAEKHQIPIAKDKRGEAPFSV
CCCCCCEEEEEEEEECCCCEEEEECCHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
DANLLHTSSEGKMLEDPAEEAPDHILQRITRPEDAPDTPEMVEITFEKGDAVAINGEPMS
CCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEECCCCCC
PATILTELNRLGGKHGIGILDLVENRFVGMKSRGIYETPGGTVLLEAHRGIEQITLDGAS
HHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEECCCCEEEEECCCCCEEEEECCCC
GHLKDSIMPRYAELIYNGFWFSPEREMLQALIDKSQEHVTGTVRLKLYKGAARCVARWSD
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHEEEEEEEEEECCHHHHHHHCCC
HSLYSEAHVTFEEDMGAYDQSDAQGFIQLNALRLKLLSARNRKFNGS
CCHHHHHCEEEHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA