Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 88658546

GI number: 88658546

Start: 462508

End: 464484

Strand: Direct

Name: priA [H]

Synonym: ECH_0483

Alternate gene names: 88658546

Gene position: 462508-464484 (Clockwise)

Preceding gene: 88658062

Following gene: 88657876

Centisome position: 39.32

GC content: 31.06

Gene sequence:

>1977_bases
ATGATAGCTGAAATTTTACTTCCGGTACCGATTAATAAGACATTTTATTACACTATTCCAGAGAATGATATGTTCTGTGT
TGGTGAATATGTTCTTGTACCTTTTGGAGCTAGAACCTTGGTAGGTGTTATATTGAAATTGAATGATATGATACCTTCAG
ATTGTAATGTAGAAAAGTTACAATTAAAAGTTGTAATTTCTAAAAATTCTTTGCCAAGAGTTAATGTTGCGTTACTTAAT
TTTATTAAATGGGTGAGCGACTATAATATTGTTCCAGCAGGTTTAGTATTAAAGATGGTGTTCAGTAATGTAGTAAATGC
TAAATCTTTTAGTAAGCTAAAATTTTATGCAAAACAAAATGACAGATGTAATAGTGTAGAAATTAATTTATCTAATGAAC
AAAGGATTGCATATAATAGTATTGTTAATAAGATTTCAGGTTATTCAGTTATAGTATTAGATGGGGAAACTGGCTCTGGA
AAAACTGAAGTTTATTGTGAAGTTATACGTGAATTAATAAAAAGGGATAGTACAGCACAAGCATTAATTTTACTTCCAGA
GATAGTGTTAACATTGCAGTTAATAAAGCGTATAAAGCATTATTTTGATAGTTATTATCCAGTAGAATGGCATTCAAATT
TAACATTAAAGAATCGTAAGGAATATTGGTTATCCATAGCATATGGGCAGTCTTTGATTGTTATAGGTGCAAGATCTGCT
TTATTTCTACCATATAAAAACCTTAAGATGATTATTGTAGATGAAGAACATGATTCATCGTTTAAGCAGGAATGTGGAGT
GCTATATAATGCACGTGATATGTCTATTGTGTTGGCAAAAAATTTGGATATACCAATAATTTTGTCTTCGGCTACTCCAT
CTCTAGAGGCGATCAACAATGTATTAAAGTCTCAGTATTATCATGTGAAATTAACGCAAAGGTTTGGAAATGCTAAATTG
CCATCAGTGAGGATTGTTGATTTATGTAAGAGCAAGATGGTCAATGTGTGGTTATCAAATAAACTTTATAATAGTATATT
GGAAACTTTGCAAAGGCAAGATCAAGTTATGCTTTTCTTGAATCGTCGTGGATATGCAAAATTGAGATTATGCAAAGCTT
GCGGTTTTAAAATAAACTGTAAAAACTGTGCTACTTGGCTTGTAGAACATAAAAAGAAAAACATATTATTATGTCATTAT
TGTGGGTATTCTTGTCCCATGCAAGATGAGTGTACTAACTGTTCAGATAAATCTTCTCTTATATCATATGGGGTAGGTGT
AGAAAAAATAGCAGAGGACATTGTTGCACTAATACCAAATGCTAAAGTTGCGATAATTAGCAGTGATATTAGTAGTAAGG
ATGTAAGTGTTATGATTGATATGATTATGCGTAATGAGGTAAATGTTATTATTGGTACTCAAGTGATAGCAAAAGGTCAT
AATTTTCCCAGATTAACGTTAGTTGGTATAATAGATGCTGATTTAAGTTTGAATAATAGTGATTTGCGTGCTACAGAGAG
AACTTATCAGCTGTTGCATCAGGTATCAGGGCGTTCTGGTAGATTTGCAGATAACGGTGAAGTAATTTTACAAACTTATG
ATACCAATAGCCCATTGATTAAATCATTGTCATCTTATAATCGTGAATTGTTTTATAGTTTAGAGCTAGAATCTAGGTTA
TTAACTAAAATGCCACCATATACAAGATTAATAGGTATAATAATTAGTGGTAAGGATGAAACTCATGTCATAAATGTGTC
GAAGCAAATAGTAAAGAGTCTTCCCCATACTTTGACTGTTTTGGGTCCAGCTCCTGCTCCTATAAGTTTTTTAAATAGGA
AATATAGATATAGAATTTTGATTAAGGTACAGCATAACGTTATTATACAAAAACTACTTGCTAAGTATAAGGAGTATTAT
GCAAAAGCAAATAAAATTAGGATTACTATAGATGTTGATCCAGTAAATTTTATGTAA

Upstream 100 bases:

>100_bases
TATTTAAAAAAATGTGTAGTTGAAAGATAGCTTTACAAGTAAAGGTGTAAATTTTGATTCTTTTGTTATAATAAAACAGC
TCTGTTATTTAAATGTTTAG

Downstream 100 bases:

>100_bases
ATTGTTATTGGGTATAGATAAGACAATAAGTTATTGTTAATGAATATTAAGAGATATTGCTTGTAGCATGTAACTAACGT
GTAATTCAGCAAGGTACATT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MIAEILLPVPINKTFYYTIPENDMFCVGEYVLVPFGARTLVGVILKLNDMIPSDCNVEKLQLKVVISKNSLPRVNVALLN
FIKWVSDYNIVPAGLVLKMVFSNVVNAKSFSKLKFYAKQNDRCNSVEINLSNEQRIAYNSIVNKISGYSVIVLDGETGSG
KTEVYCEVIRELIKRDSTAQALILLPEIVLTLQLIKRIKHYFDSYYPVEWHSNLTLKNRKEYWLSIAYGQSLIVIGARSA
LFLPYKNLKMIIVDEEHDSSFKQECGVLYNARDMSIVLAKNLDIPIILSSATPSLEAINNVLKSQYYHVKLTQRFGNAKL
PSVRIVDLCKSKMVNVWLSNKLYNSILETLQRQDQVMLFLNRRGYAKLRLCKACGFKINCKNCATWLVEHKKKNILLCHY
CGYSCPMQDECTNCSDKSSLISYGVGVEKIAEDIVALIPNAKVAIISSDISSKDVSVMIDMIMRNEVNVIIGTQVIAKGH
NFPRLTLVGIIDADLSLNNSDLRATERTYQLLHQVSGRSGRFADNGEVILQTYDTNSPLIKSLSSYNRELFYSLELESRL
LTKMPPYTRLIGIIISGKDETHVINVSKQIVKSLPHTLTVLGPAPAPISFLNRKYRYRILIKVQHNVIIQKLLAKYKEYY
AKANKIRITIDVDPVNFM

Sequences:

>Translated_658_residues
MIAEILLPVPINKTFYYTIPENDMFCVGEYVLVPFGARTLVGVILKLNDMIPSDCNVEKLQLKVVISKNSLPRVNVALLN
FIKWVSDYNIVPAGLVLKMVFSNVVNAKSFSKLKFYAKQNDRCNSVEINLSNEQRIAYNSIVNKISGYSVIVLDGETGSG
KTEVYCEVIRELIKRDSTAQALILLPEIVLTLQLIKRIKHYFDSYYPVEWHSNLTLKNRKEYWLSIAYGQSLIVIGARSA
LFLPYKNLKMIIVDEEHDSSFKQECGVLYNARDMSIVLAKNLDIPIILSSATPSLEAINNVLKSQYYHVKLTQRFGNAKL
PSVRIVDLCKSKMVNVWLSNKLYNSILETLQRQDQVMLFLNRRGYAKLRLCKACGFKINCKNCATWLVEHKKKNILLCHY
CGYSCPMQDECTNCSDKSSLISYGVGVEKIAEDIVALIPNAKVAIISSDISSKDVSVMIDMIMRNEVNVIIGTQVIAKGH
NFPRLTLVGIIDADLSLNNSDLRATERTYQLLHQVSGRSGRFADNGEVILQTYDTNSPLIKSLSSYNRELFYSLELESRL
LTKMPPYTRLIGIIISGKDETHVINVSKQIVKSLPHTLTVLGPAPAPISFLNRKYRYRILIKVQHNVIIQKLLAKYKEYY
AKANKIRITIDVDPVNFM
>Mature_658_residues
MIAEILLPVPINKTFYYTIPENDMFCVGEYVLVPFGARTLVGVILKLNDMIPSDCNVEKLQLKVVISKNSLPRVNVALLN
FIKWVSDYNIVPAGLVLKMVFSNVVNAKSFSKLKFYAKQNDRCNSVEINLSNEQRIAYNSIVNKISGYSVIVLDGETGSG
KTEVYCEVIRELIKRDSTAQALILLPEIVLTLQLIKRIKHYFDSYYPVEWHSNLTLKNRKEYWLSIAYGQSLIVIGARSA
LFLPYKNLKMIIVDEEHDSSFKQECGVLYNARDMSIVLAKNLDIPIILSSATPSLEAINNVLKSQYYHVKLTQRFGNAKL
PSVRIVDLCKSKMVNVWLSNKLYNSILETLQRQDQVMLFLNRRGYAKLRLCKACGFKINCKNCATWLVEHKKKNILLCHY
CGYSCPMQDECTNCSDKSSLISYGVGVEKIAEDIVALIPNAKVAIISSDISSKDVSVMIDMIMRNEVNVIIGTQVIAKGH
NFPRLTLVGIIDADLSLNNSDLRATERTYQLLHQVSGRSGRFADNGEVILQTYDTNSPLIKSLSSYNRELFYSLELESRL
LTKMPPYTRLIGIIISGKDETHVINVSKQIVKSLPHTLTVLGPAPAPISFLNRKYRYRILIKVQHNVIIQKLLAKYKEYY
AKANKIRITIDVDPVNFM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=544, Percent_Identity=36.3970588235294, Blast_Score=355, Evalue=6e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 74584; Mature: 74584

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAEILLPVPINKTFYYTIPENDMFCVGEYVLVPFGARTLVGVILKLNDMIPSDCNVEKL
CCCEEEEECCCCCEEEEEECCCCEEEECCEEEECCCHHHHHHHHHHHHCCCCCCCCEEEE
QLKVVISKNSLPRVNVALLNFIKWVSDYNIVPAGLVLKMVFSNVVNAKSFSKLKFYAKQN
EEEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHEEEEECCC
DRCNSVEINLSNEQRIAYNSIVNKISGYSVIVLDGETGSGKTEVYCEVIRELIKRDSTAQ
CCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
ALILLPEIVLTLQLIKRIKHYFDSYYPVEWHSNLTLKNRKEYWLSIAYGQSLIVIGARSA
EEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEECCCCEEEEEEEECCEEEEEECCCE
LFLPYKNLKMIIVDEEHDSSFKQECGVLYNARDMSIVLAKNLDIPIILSSATPSLEAINN
EEEECCCEEEEEEECCCCCCHHHHCCEEECCCCEEEEEECCCCEEEEECCCCCCHHHHHH
VLKSQYYHVKLTQRFGNAKLPSVRIVDLCKSKMVNVWLSNKLYNSILETLQRQDQVMLFL
HHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
NRRGYAKLRLCKACGFKINCKNCATWLVEHKKKNILLCHYCGYSCPMQDECTNCSDKSSL
ECCCCCEEEEHHHCCCEEECHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHH
ISYGVGVEKIAEDIVALIPNAKVAIISSDISSKDVSVMIDMIMRNEVNVIIGTQVIAKGH
HHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECEEEECCC
NFPRLTLVGIIDADLSLNNSDLRATERTYQLLHQVSGRSGRFADNGEVILQTYDTNSPLI
CCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHH
KSLSSYNRELFYSLELESRLLTKMPPYTRLIGIIISGKDETHVINVSKQIVKSLPHTLTV
HHHHHCCCEEEEEEEHHHHHHHCCCCCHHEEEEEEECCCCEEEEECHHHHHHHCCCEEEE
LGPAPAPISFLNRKYRYRILIKVQHNVIIQKLLAKYKEYYAKANKIRITIDVDPVNFM
ECCCCCCHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
>Mature Secondary Structure
MIAEILLPVPINKTFYYTIPENDMFCVGEYVLVPFGARTLVGVILKLNDMIPSDCNVEKL
CCCEEEEECCCCCEEEEEECCCCEEEECCEEEECCCHHHHHHHHHHHHCCCCCCCCEEEE
QLKVVISKNSLPRVNVALLNFIKWVSDYNIVPAGLVLKMVFSNVVNAKSFSKLKFYAKQN
EEEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHEEEEECCC
DRCNSVEINLSNEQRIAYNSIVNKISGYSVIVLDGETGSGKTEVYCEVIRELIKRDSTAQ
CCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
ALILLPEIVLTLQLIKRIKHYFDSYYPVEWHSNLTLKNRKEYWLSIAYGQSLIVIGARSA
EEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEECCCCEEEEEEEECCEEEEEECCCE
LFLPYKNLKMIIVDEEHDSSFKQECGVLYNARDMSIVLAKNLDIPIILSSATPSLEAINN
EEEECCCEEEEEEECCCCCCHHHHCCEEECCCCEEEEEECCCCEEEEECCCCCCHHHHHH
VLKSQYYHVKLTQRFGNAKLPSVRIVDLCKSKMVNVWLSNKLYNSILETLQRQDQVMLFL
HHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
NRRGYAKLRLCKACGFKINCKNCATWLVEHKKKNILLCHYCGYSCPMQDECTNCSDKSSL
ECCCCCEEEEHHHCCCEEECHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHH
ISYGVGVEKIAEDIVALIPNAKVAIISSDISSKDVSVMIDMIMRNEVNVIIGTQVIAKGH
HHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEECEEEECCC
NFPRLTLVGIIDADLSLNNSDLRATERTYQLLHQVSGRSGRFADNGEVILQTYDTNSPLI
CCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHH
KSLSSYNRELFYSLELESRLLTKMPPYTRLIGIIISGKDETHVINVSKQIVKSLPHTLTV
HHHHHCCCEEEEEEEHHHHHHHCCCCCHHEEEEEEECCCCEEEEECHHHHHHHCCCEEEE
LGPAPAPISFLNRKYRYRILIKVQHNVIIQKLLAKYKEYYAKANKIRITIDVDPVNFM
ECCCCCCHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]