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The map label for this gene is rne [H]

Identifier: 88658461

GI number: 88658461

Start: 446614

End: 448434

Strand: Direct

Name: rne [H]

Synonym: ECH_0470

Alternate gene names: 88658461

Gene position: 446614-448434 (Clockwise)

Preceding gene: 88657944

Following gene: 88658092

Centisome position: 37.97

GC content: 30.64

Gene sequence:

>1821_bases
GTGTCAAATCATAATAAAAGGATATTAATTGATGCAGTATATCAAGATGGAATTAGAGTAGCAGTATTACGTGATGGACA
GGTTGTAGAGTTTGATCAAGAATCAAAGAATAAAAAGTGTTTAAAAGGAAATGTATATTGTGCTGTAGTGAAACGTATAG
AACCATCTTTACAAGCAGTATTTATTGAATATGGAGCAAATAAACACGGGTTTTTGCCTTTTTCTGAAATATCATTAGAT
TATTATAATATTTCTGAAGAAGAGAAAGAAAAATTATATCAAGTATTATATGAAGATTCTGATGTAAAAAATGATGATGA
TGTAAAATCTGTAGATGACTCTGTTGCATGTGATACTAAGGATCAAGATAATGTTAGTGATTGTAATGACAAGTTGACAT
TATTGAAGAAAGATAATTTGCGAAATGGTAAGTCTAAATTACCTTTTTACAAATTGTATAAAGTGCAAGATGTAATAAAG
CTTGATCAAAAATTAATGGCGCAAATTGTAAAAGAGGAGCGTGGAAATAAAGGAGCAACTTTTTCAACATTTATTACCCT
GGTTGGTAGATATTGTATATTTATGCCAAATTCTGGTAATAAGAATAGTGGAATATCGCGTCGTATTGAAGATGCTGATG
ATAGAAAAATTTTAAAGAATTTTCTAAGTGCTATAAAACTGCCGAAAGAATCAGGAATAATTATTAGAACTGCTGGGTCT
AATAAGAACCAGAAAGAAATAGAGCAGGATTTATCTTATTTACAGTCGACTTGGCGAAGTATACAGAATAGTTATATAAG
TGTTACTATGCCTTTATTGTTATATGTTGAAGGTGATATAATAAAAAGAACATTACGGGATTATTGTGATGAGAATACGC
AAGATATTATTGTATCAGGTAGCTACGCATATGAGTTAGCAAAAGAATGTGTTAAGTTCATGTTTAGGAACAGGGTGCGT
CTTAGAATTTATAAGGGCAGTGTTCCTATTTTCTCTTATTACAAAATTGAGTCGCAAATTTGTGAACTATATGGAAATGT
AGTTAATTTGCCTTCTGGAGGATATATGGTTATTACTCCAACAGAAGCGTTGGTATCAATAGATATTAATTCTGGTAAGA
TGACAGGAGAGGATAGTATTGAGGAGACAGCTTATAAAACAAATATGGAAGCTGTAAAGGAAATAGCTAGACAAGTAAAC
CTGCGAGGGTTATCTGGATTAATAGTGATAGATTTTATTGATATGTTAAAATATCGCTATTGTAGGTCAGTTGAATCTGA
AATTAAAGAAGCTTTTAAATATGATAAAGCAAAAGTACAGTTTGGCTATATAAGTACATTTGGTTTGATGGAAATATCAA
GACAGAGGGTCAAGCAAAGTATATTAGAAGCGAATACGGTACAATGTTCACATTGTAAAGGAGTAGGTAGGGTTAGGTCT
TTGGAATCTTCTTCTGGAGATATATTACGTGATATTAGATATTGTGCTAATAAGAATCGTAATAAATTGATCAACATTTC
TGTTGCTGGACAAATGGTAGAGTATTTTTTTAATAATAAACGTCAACATATTGCTGAGATTGAAGAAGAATTCAGTGTGT
CAGTTAAGTTAACTGTGAATCAAAATACGAGTAGTGTATCTGATTTTAATATTAGTGTTGAAAATGATGATATGTTAAAT
CAAGGTAAGGATGCTGTTAATGTATTTACTGTTGATTATAAAATTTCTGAAGATAATAAATTAAAAGAGGAAATACAAAA
AACTCCTACTGGAAACATGTGGATAAAGAAGTGGTTGACCCGCATATTTTCACATATTTAA

Upstream 100 bases:

>100_bases
TTTTTGAGTGTAGATAAAATAAGGTGTTTTTATTTTTGAAATCTTGTCTATACTATTATAATTAAAGGCTATAGTTAAGT
GAGTTTTAGGTGTAAGTCTA

Downstream 100 bases:

>100_bases
TTCATAAATTTATGAACTTGTGTTAAATGGGGTTGTAATCTTATGATTTGGTTATTATATCTGTGATTATAGTTTTGATT
TTTATGTAAGGGGGCTATTT

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MSNHNKRILIDAVYQDGIRVAVLRDGQVVEFDQESKNKKCLKGNVYCAVVKRIEPSLQAVFIEYGANKHGFLPFSEISLD
YYNISEEEKEKLYQVLYEDSDVKNDDDVKSVDDSVACDTKDQDNVSDCNDKLTLLKKDNLRNGKSKLPFYKLYKVQDVIK
LDQKLMAQIVKEERGNKGATFSTFITLVGRYCIFMPNSGNKNSGISRRIEDADDRKILKNFLSAIKLPKESGIIIRTAGS
NKNQKEIEQDLSYLQSTWRSIQNSYISVTMPLLLYVEGDIIKRTLRDYCDENTQDIIVSGSYAYELAKECVKFMFRNRVR
LRIYKGSVPIFSYYKIESQICELYGNVVNLPSGGYMVITPTEALVSIDINSGKMTGEDSIEETAYKTNMEAVKEIARQVN
LRGLSGLIVIDFIDMLKYRYCRSVESEIKEAFKYDKAKVQFGYISTFGLMEISRQRVKQSILEANTVQCSHCKGVGRVRS
LESSSGDILRDIRYCANKNRNKLINISVAGQMVEYFFNNKRQHIAEIEEEFSVSVKLTVNQNTSSVSDFNISVENDDMLN
QGKDAVNVFTVDYKISEDNKLKEEIQKTPTGNMWIKKWLTRIFSHI

Sequences:

>Translated_606_residues
MSNHNKRILIDAVYQDGIRVAVLRDGQVVEFDQESKNKKCLKGNVYCAVVKRIEPSLQAVFIEYGANKHGFLPFSEISLD
YYNISEEEKEKLYQVLYEDSDVKNDDDVKSVDDSVACDTKDQDNVSDCNDKLTLLKKDNLRNGKSKLPFYKLYKVQDVIK
LDQKLMAQIVKEERGNKGATFSTFITLVGRYCIFMPNSGNKNSGISRRIEDADDRKILKNFLSAIKLPKESGIIIRTAGS
NKNQKEIEQDLSYLQSTWRSIQNSYISVTMPLLLYVEGDIIKRTLRDYCDENTQDIIVSGSYAYELAKECVKFMFRNRVR
LRIYKGSVPIFSYYKIESQICELYGNVVNLPSGGYMVITPTEALVSIDINSGKMTGEDSIEETAYKTNMEAVKEIARQVN
LRGLSGLIVIDFIDMLKYRYCRSVESEIKEAFKYDKAKVQFGYISTFGLMEISRQRVKQSILEANTVQCSHCKGVGRVRS
LESSSGDILRDIRYCANKNRNKLINISVAGQMVEYFFNNKRQHIAEIEEEFSVSVKLTVNQNTSSVSDFNISVENDDMLN
QGKDAVNVFTVDYKISEDNKLKEEIQKTPTGNMWIKKWLTRIFSHI
>Mature_605_residues
SNHNKRILIDAVYQDGIRVAVLRDGQVVEFDQESKNKKCLKGNVYCAVVKRIEPSLQAVFIEYGANKHGFLPFSEISLDY
YNISEEEKEKLYQVLYEDSDVKNDDDVKSVDDSVACDTKDQDNVSDCNDKLTLLKKDNLRNGKSKLPFYKLYKVQDVIKL
DQKLMAQIVKEERGNKGATFSTFITLVGRYCIFMPNSGNKNSGISRRIEDADDRKILKNFLSAIKLPKESGIIIRTAGSN
KNQKEIEQDLSYLQSTWRSIQNSYISVTMPLLLYVEGDIIKRTLRDYCDENTQDIIVSGSYAYELAKECVKFMFRNRVRL
RIYKGSVPIFSYYKIESQICELYGNVVNLPSGGYMVITPTEALVSIDINSGKMTGEDSIEETAYKTNMEAVKEIARQVNL
RGLSGLIVIDFIDMLKYRYCRSVESEIKEAFKYDKAKVQFGYISTFGLMEISRQRVKQSILEANTVQCSHCKGVGRVRSL
ESSSGDILRDIRYCANKNRNKLINISVAGQMVEYFFNNKRQHIAEIEEEFSVSVKLTVNQNTSSVSDFNISVENDDMLNQ
GKDAVNVFTVDYKISEDNKLKEEIQKTPTGNMWIKKWLTRIFSHI

Specific function: This protein matures 5S rRNA from its precursors from all the rRNA genes. It is the major endoribonuclease participating in mRNA turnover [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=376, Percent_Identity=43.3510638297872, Blast_Score=319, Evalue=4e-88,
Organism=Escherichia coli, GI87082235, Length=511, Percent_Identity=28.7671232876712, Blast_Score=204, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 69382; Mature: 69251

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNHNKRILIDAVYQDGIRVAVLRDGQVVEFDQESKNKKCLKGNVYCAVVKRIEPSLQAV
CCCCCCEEEEEEEECCCEEEEEEECCCEEEECCCCCCCCCCCCCEEEEEEHHCCCCCEEE
FIEYGANKHGFLPFSEISLDYYNISEEEKEKLYQVLYEDSDVKNDDDVKSVDDSVACDTK
EEEECCCCCCCCCHHHCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCC
DQDNVSDCNDKLTLLKKDNLRNGKSKLPFYKLYKVQDVIKLDQKLMAQIVKEERGNKGAT
CCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
FSTFITLVGRYCIFMPNSGNKNSGISRRIEDADDRKILKNFLSAIKLPKESGIIIRTAGS
HHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCC
NKNQKEIEQDLSYLQSTWRSIQNSYISVTMPLLLYVEGDIIKRTLRDYCDENTQDIIVSG
CCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCHHHHHHHHHHHCCCCCEEEEEC
SYAYELAKECVKFMFRNRVRLRIYKGSVPIFSYYKIESQICELYGNVVNLPSGGYMVITP
HHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEHHHHHHHHHHHCCEEECCCCCEEEEEC
TEALVSIDINSGKMTGEDSIEETAYKTNMEAVKEIARQVNLRGLSGLIVIDFIDMLKYRY
CCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
CRSVESEIKEAFKYDKAKVQFGYISTFGLMEISRQRVKQSILEANTVQCSHCKGVGRVRS
HHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHH
LESSSGDILRDIRYCANKNRNKLINISVAGQMVEYFFNNKRQHIAEIEEEFSVSVKLTVN
CCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHCEEEEEEEEC
QNTSSVSDFNISVENDDMLNQGKDAVNVFTVDYKISEDNKLKEEIQKTPTGNMWIKKWLT
CCCCCCCEEEEEECCCHHHHCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHH
RIFSHI
HHHHCC
>Mature Secondary Structure 
SNHNKRILIDAVYQDGIRVAVLRDGQVVEFDQESKNKKCLKGNVYCAVVKRIEPSLQAV
CCCCCEEEEEEEECCCEEEEEEECCCEEEECCCCCCCCCCCCCEEEEEEHHCCCCCEEE
FIEYGANKHGFLPFSEISLDYYNISEEEKEKLYQVLYEDSDVKNDDDVKSVDDSVACDTK
EEEECCCCCCCCCHHHCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCC
DQDNVSDCNDKLTLLKKDNLRNGKSKLPFYKLYKVQDVIKLDQKLMAQIVKEERGNKGAT
CCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
FSTFITLVGRYCIFMPNSGNKNSGISRRIEDADDRKILKNFLSAIKLPKESGIIIRTAGS
HHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCC
NKNQKEIEQDLSYLQSTWRSIQNSYISVTMPLLLYVEGDIIKRTLRDYCDENTQDIIVSG
CCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCHHHHHHHHHHHCCCCCEEEEEC
SYAYELAKECVKFMFRNRVRLRIYKGSVPIFSYYKIESQICELYGNVVNLPSGGYMVITP
HHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEHHHHHHHHHHHCCEEECCCCCEEEEEC
TEALVSIDINSGKMTGEDSIEETAYKTNMEAVKEIARQVNLRGLSGLIVIDFIDMLKYRY
CCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
CRSVESEIKEAFKYDKAKVQFGYISTFGLMEISRQRVKQSILEANTVQCSHCKGVGRVRS
HHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHH
LESSSGDILRDIRYCANKNRNKLINISVAGQMVEYFFNNKRQHIAEIEEEFSVSVKLTVN
CCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHCEEEEEEEEC
QNTSSVSDFNISVENDDMLNQGKDAVNVFTVDYKISEDNKLKEEIQKTPTGNMWIKKWLT
CCCCCCCEEEEEECCCHHHHCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHH
RIFSHI
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]