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The map label for this gene is hemK [H]

Identifier: 88658386

GI number: 88658386

Start: 1090653

End: 1091495

Strand: Reverse

Name: hemK [H]

Synonym: ECH_1063

Alternate gene names: 88658386

Gene position: 1091495-1090653 (Counterclockwise)

Preceding gene: 88657622

Following gene: 88658244

Centisome position: 92.79

GC content: 30.6

Gene sequence:

>843_bases
ATGGATACTGTAATTTCTTTATTTAATAGAGCTGTTGATTTTTTATCTACTTATAACATAGACAATCCAAAACGTGATGT
AGAGATAATAATAAAGCATCTTATTAACACTGAAAACTTAGTTACTATACTTGATCCACATATGCCTATTAATAAATGTG
ATGTGGAAGTATTTTGGAAAATGGTGAAACAACGTTCTACTAATATACCTATATCACATATTATAGGTAAAAGGGAATTT
TGGAGTACGGATTTTATTGTCAATTCTGATGTTTTAGATCCTAGACCAGATAGTGAAACGATCATTTCCTCTGTGTTTGC
AATGTATCCTTGTAAGAATCGTAGGTTAATTATAGGAGATTTTGGTACAGGGAGTGGATGTTTATTATCAATTTTATTAT
TAAGGTATAGAAATGCTGTTGGTATAGCTATTGAAAAAAGTGTTAAAGCTTATAGAGTTGCATATCAGAATTTTAAAAAT
CATGGATTATATAGTAGGATAAAGATGAGATTATCCTCTTGGAACACTTGCTATGATACTTTTGATTTGATAGTTAGTAA
TCCTCCGTATATTAGAAGGAGTAAAATTACTACATTACAGCCGGAAGTAAAATTACATGAGCCAATGGTAGCTTTGGATG
GTGGGCCAATAGGATTAGAAATTTATTCACAAATTTTTGTAATCATAAAAAGATGTTTAAAGCAGAATGGTGTAGCTATT
TTGGAAATAGGGGAAGATCAACATCAGATTCATAGAATAGTGCATCGTTATGGATTAAAATTCTGTACTTACCACAATGA
TTTATCTGGCAAGTTACGTTGCATAGTAGTTAAACAGATGTGA

Upstream 100 bases:

>100_bases
GCAATTTGAACATTCTTTGGGTGTTACTGAGAATGGAGTAGAAATATTTACATTATCTCCACAAAATATGCATTATCCTC
CTTATGTATGCTAATTTATT

Downstream 100 bases:

>100_bases
AATTAATTAGGGCTAATGTTTTAAGGTTAATGTGAAGAAAGGTTAGTATTTTATGGTTACATAAACCTAGGTATAGCTAT
TTTGGAAATAGGGGAAGTCA

Product: HemK family modification methylase

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MDTVISLFNRAVDFLSTYNIDNPKRDVEIIIKHLINTENLVTILDPHMPINKCDVEVFWKMVKQRSTNIPISHIIGKREF
WSTDFIVNSDVLDPRPDSETIISSVFAMYPCKNRRLIIGDFGTGSGCLLSILLLRYRNAVGIAIEKSVKAYRVAYQNFKN
HGLYSRIKMRLSSWNTCYDTFDLIVSNPPYIRRSKITTLQPEVKLHEPMVALDGGPIGLEIYSQIFVIIKRCLKQNGVAI
LEIGEDQHQIHRIVHRYGLKFCTYHNDLSGKLRCIVVKQM

Sequences:

>Translated_280_residues
MDTVISLFNRAVDFLSTYNIDNPKRDVEIIIKHLINTENLVTILDPHMPINKCDVEVFWKMVKQRSTNIPISHIIGKREF
WSTDFIVNSDVLDPRPDSETIISSVFAMYPCKNRRLIIGDFGTGSGCLLSILLLRYRNAVGIAIEKSVKAYRVAYQNFKN
HGLYSRIKMRLSSWNTCYDTFDLIVSNPPYIRRSKITTLQPEVKLHEPMVALDGGPIGLEIYSQIFVIIKRCLKQNGVAI
LEIGEDQHQIHRIVHRYGLKFCTYHNDLSGKLRCIVVKQM
>Mature_280_residues
MDTVISLFNRAVDFLSTYNIDNPKRDVEIIIKHLINTENLVTILDPHMPINKCDVEVFWKMVKQRSTNIPISHIIGKREF
WSTDFIVNSDVLDPRPDSETIISSVFAMYPCKNRRLIIGDFGTGSGCLLSILLLRYRNAVGIAIEKSVKAYRVAYQNFKN
HGLYSRIKMRLSSWNTCYDTFDLIVSNPPYIRRSKITTLQPEVKLHEPMVALDGGPIGLEIYSQIFVIIKRCLKQNGVAI
LEIGEDQHQIHRIVHRYGLKFCTYHNDLSGKLRCIVVKQM

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=261, Percent_Identity=24.904214559387, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1787463, Length=248, Percent_Identity=27.0161290322581, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082085, Length=187, Percent_Identity=31.0160427807487, Blast_Score=82, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24582226, Length=281, Percent_Identity=26.3345195729537, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32243; Mature: 32243

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVISLFNRAVDFLSTYNIDNPKRDVEIIIKHLINTENLVTILDPHMPINKCDVEVFWK
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
MVKQRSTNIPISHIIGKREFWSTDFIVNSDVLDPRPDSETIISSVFAMYPCKNRRLIIGD
HHHHCCCCCCHHHHHCCHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
FGTGSGCLLSILLLRYRNAVGIAIEKSVKAYRVAYQNFKNHGLYSRIKMRLSSWNTCYDT
CCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHH
FDLIVSNPPYIRRSKITTLQPEVKLHEPMVALDGGPIGLEIYSQIFVIIKRCLKQNGVAI
HHHHCCCCCCEECCCCEEECCCCEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEE
LEIGEDQHQIHRIVHRYGLKFCTYHNDLSGKLRCIVVKQM
EEECCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECC
>Mature Secondary Structure
MDTVISLFNRAVDFLSTYNIDNPKRDVEIIIKHLINTENLVTILDPHMPINKCDVEVFWK
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHH
MVKQRSTNIPISHIIGKREFWSTDFIVNSDVLDPRPDSETIISSVFAMYPCKNRRLIIGD
HHHHCCCCCCHHHHHCCHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEE
FGTGSGCLLSILLLRYRNAVGIAIEKSVKAYRVAYQNFKNHGLYSRIKMRLSSWNTCYDT
CCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHH
FDLIVSNPPYIRRSKITTLQPEVKLHEPMVALDGGPIGLEIYSQIFVIIKRCLKQNGVAI
HHHHCCCCCCEECCCCEEECCCCEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEE
LEIGEDQHQIHRIVHRYGLKFCTYHNDLSGKLRCIVVKQM
EEECCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]