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The map label for this gene is dnaB [H]

Identifier: 88658191

GI number: 88658191

Start: 427624

End: 429090

Strand: Direct

Name: dnaB [H]

Synonym: ECH_0451

Alternate gene names: 88658191

Gene position: 427624-429090 (Clockwise)

Preceding gene: 88657817

Following gene: 88657874

Centisome position: 36.35

GC content: 32.04

Gene sequence:

>1467_bases
TTGGAATTAAAATCAAACACTAGTAAGAATTTTGGCCAATTAATGTCTGAGCTTCCCTGTAATATTGAAGCTGAGCAAAT
ACTATTGGGTTCAATGTTGCGTGATAATAGAATTTGTGATGCGGTTGAAGATATAATAACAAGTGATGTGTTTTTTGATA
GTCTTCATAGGCGTATTTTTGAACAAATAGTAAAAATTAATAAGCGTGGATTGGTCGCTAATGAAGTTAGCTTAAAAATG
TTTTTTGATAATGATGAAGATTTAATTGAATCTGGTGGAGTTGACTATTTAGCAAAAATTGCAGCAAAAGCTAGTATTAT
TTTTGATATTAAGAATGTTGCAAATATAGTTGTTGATACTTATTTGAGGAGATGCCTGATTTCATTTGGGCAAGATTTAA
TTAGTGGTGCATATAATTATGATGTTGATAATTCTGCAGTTTATCAAATAGAAAGTGCTGCGCAAAAGTTATTCTTACTT
GGCAATCAAGGAAAATCTGATAAAACTTATCATCAATTTGCACTTTTAGTTGCAAATATTAAGAAAAGAATAGAATTTGC
AAAAAATAATGAAGGGACATTGCAAGGTGTGTCTGTAGGGTTTCGTGATTTAGATCAGCTGCTAAATGGATTACAGAAGT
CTGATTTACTTATTTTAGCTGCACGTCCATCTATGGGTAAGACAGCTTTAGCACTAAATATGGCATTAAATGCATGTAAA
ATTTTAAGTAATAGTAGTCAGTACAATGGGCAACATATTGGTTTTTTTTCATTAGAAATGTCAGCGGAGCAGTTGGCTTC
TAGGGTAATAGCAATTGAATCAGATGTAAGTTCTTATAAAGCATTATCTGGAAAAATTAGTGGGGAAGAACTTAAAAAAG
TACTTGATGCTAGTACGAAGGTATGTAATTTGCCGTTAATTATTGATGATTCATCTAGTATTTCTATAAGTAGCTTAAGA
ACTAGAATTCGTAGGATGCATCAGCTGTATAATTTGGAAGCTGTTTTTATAGATTACTTGCAATTAGTCAAGGGTACGAC
TAAACGCAGTGGGGAAAATAGAGTTCAGGAGGTATCAGAAGTTACACAAGGGTTAAAAGCTATTGCAAAAGAATTAAATA
TACCGGTTGTAGCCTTATCTCAATTATCAAGGTTAGTTGAGCAGCGTGATGATAAAAAGCCTCAGTTATCGGATTTAAGG
GATTCTGGTAGTATAGAACAGGATGCTGATGTTGTAATGTTTTTATATAGAGAAGAATACTATGAACTTAGAAAACAGCC
ATCTGAAGGTACAGCTAAACATGTTGAATGGCAAGAAAGGATGAATTCAATAAGTAATGTTGCAGATATATTGATTTCAA
AACAGCGTAATGGTCCAATTGGAAATATTAAATTATTTTTTGAAGCAGAAAAAGGTAGATTTAGGGATTACACAGCAAAA
TATAATCCTGCAGATTTTAATTTTTAA

Upstream 100 bases:

>100_bases
TAATTGGGTTTTTTTAAGTTGTTAGTGACTGTAAGATGTTATAAATAGTTATAATCGAGTTTTTATCTAAATTGTAGTAA
TGTTATTTTGAGAGGGTATT

Downstream 100 bases:

>100_bases
AATTCGTTTTTTTATACTGTGGCTTTCTAGATAGAGATCACTGTATAAAATGAGTAGGTTTATTTTTGCAAATAGTAGTA
GTAGTGATCTTACGTACAAG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MELKSNTSKNFGQLMSELPCNIEAEQILLGSMLRDNRICDAVEDIITSDVFFDSLHRRIFEQIVKINKRGLVANEVSLKM
FFDNDEDLIESGGVDYLAKIAAKASIIFDIKNVANIVVDTYLRRCLISFGQDLISGAYNYDVDNSAVYQIESAAQKLFLL
GNQGKSDKTYHQFALLVANIKKRIEFAKNNEGTLQGVSVGFRDLDQLLNGLQKSDLLILAARPSMGKTALALNMALNACK
ILSNSSQYNGQHIGFFSLEMSAEQLASRVIAIESDVSSYKALSGKISGEELKKVLDASTKVCNLPLIIDDSSSISISSLR
TRIRRMHQLYNLEAVFIDYLQLVKGTTKRSGENRVQEVSEVTQGLKAIAKELNIPVVALSQLSRLVEQRDDKKPQLSDLR
DSGSIEQDADVVMFLYREEYYELRKQPSEGTAKHVEWQERMNSISNVADILISKQRNGPIGNIKLFFEAEKGRFRDYTAK
YNPADFNF

Sequences:

>Translated_488_residues
MELKSNTSKNFGQLMSELPCNIEAEQILLGSMLRDNRICDAVEDIITSDVFFDSLHRRIFEQIVKINKRGLVANEVSLKM
FFDNDEDLIESGGVDYLAKIAAKASIIFDIKNVANIVVDTYLRRCLISFGQDLISGAYNYDVDNSAVYQIESAAQKLFLL
GNQGKSDKTYHQFALLVANIKKRIEFAKNNEGTLQGVSVGFRDLDQLLNGLQKSDLLILAARPSMGKTALALNMALNACK
ILSNSSQYNGQHIGFFSLEMSAEQLASRVIAIESDVSSYKALSGKISGEELKKVLDASTKVCNLPLIIDDSSSISISSLR
TRIRRMHQLYNLEAVFIDYLQLVKGTTKRSGENRVQEVSEVTQGLKAIAKELNIPVVALSQLSRLVEQRDDKKPQLSDLR
DSGSIEQDADVVMFLYREEYYELRKQPSEGTAKHVEWQERMNSISNVADILISKQRNGPIGNIKLFFEAEKGRFRDYTAK
YNPADFNF
>Mature_488_residues
MELKSNTSKNFGQLMSELPCNIEAEQILLGSMLRDNRICDAVEDIITSDVFFDSLHRRIFEQIVKINKRGLVANEVSLKM
FFDNDEDLIESGGVDYLAKIAAKASIIFDIKNVANIVVDTYLRRCLISFGQDLISGAYNYDVDNSAVYQIESAAQKLFLL
GNQGKSDKTYHQFALLVANIKKRIEFAKNNEGTLQGVSVGFRDLDQLLNGLQKSDLLILAARPSMGKTALALNMALNACK
ILSNSSQYNGQHIGFFSLEMSAEQLASRVIAIESDVSSYKALSGKISGEELKKVLDASTKVCNLPLIIDDSSSISISSLR
TRIRRMHQLYNLEAVFIDYLQLVKGTTKRSGENRVQEVSEVTQGLKAIAKELNIPVVALSQLSRLVEQRDDKKPQLSDLR
DSGSIEQDADVVMFLYREEYYELRKQPSEGTAKHVEWQERMNSISNVADILISKQRNGPIGNIKLFFEAEKGRFRDYTAK
YNPADFNF

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=478, Percent_Identity=34.7280334728033, Blast_Score=254, Evalue=6e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54753; Mature: 54753

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKSNTSKNFGQLMSELPCNIEAEQILLGSMLRDNRICDAVEDIITSDVFFDSLHRRIF
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
EQIVKINKRGLVANEVSLKMFFDNDEDLIESGGVDYLAKIAAKASIIFDIKNVANIVVDT
HHHHHHCCCCCEEECEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHEEEHHHHHHHHHHH
YLRRCLISFGQDLISGAYNYDVDNSAVYQIESAAQKLFLLGNQGKSDKTYHQFALLVANI
HHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHH
KKRIEFAKNNEGTLQGVSVGFRDLDQLLNGLQKSDLLILAARPSMGKTALALNMALNACK
HHHHHHHCCCCCCEEEHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
ILSNSSQYNGQHIGFFSLEMSAEQLASRVIAIESDVSSYKALSGKISGEELKKVLDASTK
HHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCC
VCNLPLIIDDSSSISISSLRTRIRRMHQLYNLEAVFIDYLQLVKGTTKRSGENRVQEVSE
EEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VTQGLKAIAKELNIPVVALSQLSRLVEQRDDKKPQLSDLRDSGSIEQDADVVMFLYREEY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHH
YELRKQPSEGTAKHVEWQERMNSISNVADILISKQRNGPIGNIKLFFEAEKGRFRDYTAK
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCC
YNPADFNF
CCCCCCCC
>Mature Secondary Structure
MELKSNTSKNFGQLMSELPCNIEAEQILLGSMLRDNRICDAVEDIITSDVFFDSLHRRIF
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
EQIVKINKRGLVANEVSLKMFFDNDEDLIESGGVDYLAKIAAKASIIFDIKNVANIVVDT
HHHHHHCCCCCEEECEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHEEEHHHHHHHHHHH
YLRRCLISFGQDLISGAYNYDVDNSAVYQIESAAQKLFLLGNQGKSDKTYHQFALLVANI
HHHHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHH
KKRIEFAKNNEGTLQGVSVGFRDLDQLLNGLQKSDLLILAARPSMGKTALALNMALNACK
HHHHHHHCCCCCCEEEHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
ILSNSSQYNGQHIGFFSLEMSAEQLASRVIAIESDVSSYKALSGKISGEELKKVLDASTK
HHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHCCCC
VCNLPLIIDDSSSISISSLRTRIRRMHQLYNLEAVFIDYLQLVKGTTKRSGENRVQEVSE
EEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VTQGLKAIAKELNIPVVALSQLSRLVEQRDDKKPQLSDLRDSGSIEQDADVVMFLYREEY
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHH
YELRKQPSEGTAKHVEWQERMNSISNVADILISKQRNGPIGNIKLFFEAEKGRFRDYTAK
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCC
YNPADFNF
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA