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The map label for this gene is nrdA [H]

Identifier: 88658153

GI number: 88658153

Start: 434039

End: 435826

Strand: Direct

Name: nrdA [H]

Synonym: ECH_0459

Alternate gene names: 88658153

Gene position: 434039-435826 (Clockwise)

Preceding gene: 88658469

Following gene: 88658457

Centisome position: 36.9

GC content: 32.05

Gene sequence:

>1788_bases
ATGGGGACAGTAATTGTTGATTATGAAAGAGATAATAATCTTACAGCTTTTGGCAAAGCTGTATTGTCTGATAGGTATTT
ACTTGAAAATGAAGATTATCAACAACTGTTTGTAAGAATTTCTGAATATTATTCAGACAATCCAGAACATGCACAAAGGT
TGTATGATTATATGAGTCAACTATGGTTTATGCCTGCTACTCCTATACTAAGTAATGGTGGTACAAATAGAGGGTTGCCA
GTTTCATGTTTTCTCAATGAAACAGAAGATAGTTTAAGAGGTATTGTTAATTTATGGAATGAAAATGTATGGTTGGCATC
TAAGGGTGGTGGAATAGGAAGTTATTGGGGAAATTTGCGTTCTATAGGTGAAAAAGTACGTGGTAGTGGTAAAACTTCTG
GTATTATACCGTTTATTGTAGTCCAAAATGCTTTAACTTTGGCAATTAGTCAAGGATCATTACGTCGGGGTAGTTCTGCT
GTATATTTGCCTGTTTGGCATCCAGAGATTGAAGAATTCTTAGATTTAAGAAAACCAACAGGTGGTGACCCTAATAGAAA
AGCATTAAATATACATCATGGGGTTGTATTATCAAATAAATTCATGAAATGTGTAGAAAATGATGAACCATGGGATTTGA
TCAGCCCTAAAGATCAGTCGGTACTTTTTACTATAAAAGCACGTGATATATGGATAAAAATTTTGACAACAAGAGTTGAA
ACAGGTGAGCCATATATATTATTTATTGACCAAGTTGATAACAATAAACCTGAAATTTATAAAAGACTGAATTTAGACAT
AAAAATGTCTAATTTATGTACAGAAATAACTCTCACTACTGGATATGATCATTTAAATAAGTCACGTACTGCTATATGTT
GTCTTGCATCATTGAATCTTGAATATTATGAACAATGGAAGGACAATTCGTTGTTTATTGAAGATGTTATGAGGTTTCTT
GATAATGTGCTAACTGATTTTATAGAAAAAGCTCCTAGTGAAATGGAAAGAGCAAAATATTCTGCGATGCGTGAACGTAG
TATAGGAGTTGGAGTGATGGGGTTCCATTCATTTTTGCAAAGTAAAATGATACCCTTTGAATCTGTAGCAGCTACAATAT
GGAATAAAAAGATATTTTCTCATATAAGAAAACATGCAGATGCTGCGTCTTATAATATTGCTATGGAAAAAGGTTCTTGT
CTTGATGCACAAGATGCTGGTATTGTAGAGAGGTTTGTAAATAAGTTATCTATTGCTCCTACTGCATCAATTTCTATTAT
TGCTGGAAATACTTCTCCTGGTATTGAACCATATGCAGCAAATGTTTTTACACATAAAACTTTAACTGGTTCTTTTATTG
TAAGAAATAAGTTTTTGCAAAAATTGTTAAAGCTAAAAGGTAAAGATAATGAACAGGTATGGTCTTCTATATCAACTAAT
GAGGGATCTGTACAGCATTTGGATTTTTTAACTGAAGAAGAAAAATTAATATTTAAAACTGCATATGAACTTGATCAAAG
GTGGATAATTGAGCATACAAGCGATAGATCAAAATATATATGCCAGGCACAATCTGTAAATATTTTTCTTCCTGCTAATG
TTCACAAAAGGTATTTGCATAAAATACATTTTCTTGCTTGGAGGAAAGGATTGAAAAGTTTGTATTACTGTAGATCAAAA
TCAATCCAACGTGCAGATAAAGTATCTCATGGGGCATTAGAAAAATCAATTGAACAATATAAGCAAATTACAAACTTTGA
TTATAATGAGTGTTTATCTTGTCAGTAG

Upstream 100 bases:

>100_bases
TCTATATATTGTGCTTTATATAGTAAATATATACAATATGTATGCGTGAGCAATAATTCCTATTATGCATAGGGTTTGAA
TAATAAGAAAGGTAGAGATT

Downstream 100 bases:

>100_bases
TAGATCTTATTATTAAGTAATTACTGTTCTTCATTATTTGCAGTTAGTATACTGCTGCTATATCATACTGAGCGTTTATT
TTACTAGTGCATGTTATAAA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase [H]

Number of amino acids: Translated: 595; Mature: 594

Protein sequence:

>595_residues
MGTVIVDYERDNNLTAFGKAVLSDRYLLENEDYQQLFVRISEYYSDNPEHAQRLYDYMSQLWFMPATPILSNGGTNRGLP
VSCFLNETEDSLRGIVNLWNENVWLASKGGGIGSYWGNLRSIGEKVRGSGKTSGIIPFIVVQNALTLAISQGSLRRGSSA
VYLPVWHPEIEEFLDLRKPTGGDPNRKALNIHHGVVLSNKFMKCVENDEPWDLISPKDQSVLFTIKARDIWIKILTTRVE
TGEPYILFIDQVDNNKPEIYKRLNLDIKMSNLCTEITLTTGYDHLNKSRTAICCLASLNLEYYEQWKDNSLFIEDVMRFL
DNVLTDFIEKAPSEMERAKYSAMRERSIGVGVMGFHSFLQSKMIPFESVAATIWNKKIFSHIRKHADAASYNIAMEKGSC
LDAQDAGIVERFVNKLSIAPTASISIIAGNTSPGIEPYAANVFTHKTLTGSFIVRNKFLQKLLKLKGKDNEQVWSSISTN
EGSVQHLDFLTEEEKLIFKTAYELDQRWIIEHTSDRSKYICQAQSVNIFLPANVHKRYLHKIHFLAWRKGLKSLYYCRSK
SIQRADKVSHGALEKSIEQYKQITNFDYNECLSCQ

Sequences:

>Translated_595_residues
MGTVIVDYERDNNLTAFGKAVLSDRYLLENEDYQQLFVRISEYYSDNPEHAQRLYDYMSQLWFMPATPILSNGGTNRGLP
VSCFLNETEDSLRGIVNLWNENVWLASKGGGIGSYWGNLRSIGEKVRGSGKTSGIIPFIVVQNALTLAISQGSLRRGSSA
VYLPVWHPEIEEFLDLRKPTGGDPNRKALNIHHGVVLSNKFMKCVENDEPWDLISPKDQSVLFTIKARDIWIKILTTRVE
TGEPYILFIDQVDNNKPEIYKRLNLDIKMSNLCTEITLTTGYDHLNKSRTAICCLASLNLEYYEQWKDNSLFIEDVMRFL
DNVLTDFIEKAPSEMERAKYSAMRERSIGVGVMGFHSFLQSKMIPFESVAATIWNKKIFSHIRKHADAASYNIAMEKGSC
LDAQDAGIVERFVNKLSIAPTASISIIAGNTSPGIEPYAANVFTHKTLTGSFIVRNKFLQKLLKLKGKDNEQVWSSISTN
EGSVQHLDFLTEEEKLIFKTAYELDQRWIIEHTSDRSKYICQAQSVNIFLPANVHKRYLHKIHFLAWRKGLKSLYYCRSK
SIQRADKVSHGALEKSIEQYKQITNFDYNECLSCQ
>Mature_594_residues
GTVIVDYERDNNLTAFGKAVLSDRYLLENEDYQQLFVRISEYYSDNPEHAQRLYDYMSQLWFMPATPILSNGGTNRGLPV
SCFLNETEDSLRGIVNLWNENVWLASKGGGIGSYWGNLRSIGEKVRGSGKTSGIIPFIVVQNALTLAISQGSLRRGSSAV
YLPVWHPEIEEFLDLRKPTGGDPNRKALNIHHGVVLSNKFMKCVENDEPWDLISPKDQSVLFTIKARDIWIKILTTRVET
GEPYILFIDQVDNNKPEIYKRLNLDIKMSNLCTEITLTTGYDHLNKSRTAICCLASLNLEYYEQWKDNSLFIEDVMRFLD
NVLTDFIEKAPSEMERAKYSAMRERSIGVGVMGFHSFLQSKMIPFESVAATIWNKKIFSHIRKHADAASYNIAMEKGSCL
DAQDAGIVERFVNKLSIAPTASISIIAGNTSPGIEPYAANVFTHKTLTGSFIVRNKFLQKLLKLKGKDNEQVWSSISTNE
GSVQHLDFLTEEEKLIFKTAYELDQRWIIEHTSDRSKYICQAQSVNIFLPANVHKRYLHKIHFLAWRKGLKSLYYCRSKS
IQRADKVSHGALEKSIEQYKQITNFDYNECLSCQ

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 ATP-cone domains [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=618, Percent_Identity=30.5825242718447, Blast_Score=255, Evalue=7e-68,
Organism=Escherichia coli, GI1789030, Length=598, Percent_Identity=26.7558528428094, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI2367133, Length=440, Percent_Identity=25.9090909090909, Blast_Score=125, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17555242, Length=618, Percent_Identity=31.5533980582524, Blast_Score=257, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6320914, Length=658, Percent_Identity=30.8510638297872, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322123, Length=627, Percent_Identity=30.7814992025518, Blast_Score=239, Evalue=8e-64,
Organism=Drosophila melanogaster, GI17352481, Length=624, Percent_Identity=29.0064102564103, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 67880; Mature: 67749

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTVIVDYERDNNLTAFGKAVLSDRYLLENEDYQQLFVRISEYYSDNPEHAQRLYDYMSQ
CCCEEEEEECCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LWFMPATPILSNGGTNRGLPVSCFLNETEDSLRGIVNLWNENVWLASKGGGIGSYWGNLR
HHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
SIGEKVRGSGKTSGIIPFIVVQNALTLAISQGSLRRGSSAVYLPVWHPEIEEFLDLRKPT
HHHHHHCCCCCCCCCCHHEECCCCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHCCCC
GGDPNRKALNIHHGVVLSNKFMKCVENDEPWDLISPKDQSVLFTIKARDIWIKILTTRVE
CCCCCCCEEEEECCEEECHHHHHHHCCCCCCCCCCCCCCEEEEEEEECEEEEEEEEEEEC
TGEPYILFIDQVDNNKPEIYKRLNLDIKMSNLCTEITLTTGYDHLNKSRTAICCLASLNL
CCCCEEEEEECCCCCCCHHHHHHCCCEEECCCEEEEEEECCCHHCCCCCCEEEEEECCCH
EYYEQWKDNSLFIEDVMRFLDNVLTDFIEKAPSEMERAKYSAMRERSIGVGVMGFHSFLQ
HHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SKMIPFESVAATIWNKKIFSHIRKHADAASYNIAMEKGSCLDAQDAGIVERFVNKLSIAP
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCC
TASISIIAGNTSPGIEPYAANVFTHKTLTGSFIVRNKFLQKLLKLKGKDNEQVWSSISTN
CCEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCC
EGSVQHLDFLTEEEKLIFKTAYELDQRWIIEHTSDRSKYICQAQSVNIFLPANVHKRYLH
CCCCHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCEEEEECCCHHHHHHH
KIHFLAWRKGLKSLYYCRSKSIQRADKVSHGALEKSIEQYKQITNFDYNECLSCQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
GTVIVDYERDNNLTAFGKAVLSDRYLLENEDYQQLFVRISEYYSDNPEHAQRLYDYMSQ
CCEEEEEECCCCCHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LWFMPATPILSNGGTNRGLPVSCFLNETEDSLRGIVNLWNENVWLASKGGGIGSYWGNLR
HHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
SIGEKVRGSGKTSGIIPFIVVQNALTLAISQGSLRRGSSAVYLPVWHPEIEEFLDLRKPT
HHHHHHCCCCCCCCCCHHEECCCCEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHCCCC
GGDPNRKALNIHHGVVLSNKFMKCVENDEPWDLISPKDQSVLFTIKARDIWIKILTTRVE
CCCCCCCEEEEECCEEECHHHHHHHCCCCCCCCCCCCCCEEEEEEEECEEEEEEEEEEEC
TGEPYILFIDQVDNNKPEIYKRLNLDIKMSNLCTEITLTTGYDHLNKSRTAICCLASLNL
CCCCEEEEEECCCCCCCHHHHHHCCCEEECCCEEEEEEECCCHHCCCCCCEEEEEECCCH
EYYEQWKDNSLFIEDVMRFLDNVLTDFIEKAPSEMERAKYSAMRERSIGVGVMGFHSFLQ
HHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
SKMIPFESVAATIWNKKIFSHIRKHADAASYNIAMEKGSCLDAQDAGIVERFVNKLSIAP
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCC
TASISIIAGNTSPGIEPYAANVFTHKTLTGSFIVRNKFLQKLLKLKGKDNEQVWSSISTN
CCEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCC
EGSVQHLDFLTEEEKLIFKTAYELDQRWIIEHTSDRSKYICQAQSVNIFLPANVHKRYLH
CCCCHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCEEEEECCCHHHHHHH
KIHFLAWRKGLKSLYYCRSKSIQRADKVSHGALEKSIEQYKQITNFDYNECLSCQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]