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The map label for this gene is yleA [C]

Identifier: 88658127

GI number: 88658127

Start: 204031

End: 205269

Strand: Direct

Name: yleA [C]

Synonym: ECH_0217

Alternate gene names: 88658127

Gene position: 204031-205269 (Clockwise)

Preceding gene: 88658643

Following gene: 88657851

Centisome position: 17.35

GC content: 31.48

Gene sequence:

>1239_bases
ATGAGTGACGTAATTACCTTTGGATGTAGATTAAATTTTTATGAAAGTGAAGTTATAAAAAATAACTTAAAAAAGGCTCA
GTTGGATGATGTTATAGTTGTTCATAGTTGTGCTGTAACTAATGAAGCTGAACGTCAAGTAAGGTCTAGGATACGTAAAC
TTTATAGAGATAATGCTAATGTTAAAATTATTGTAGCAGGCTGTGCTGTTCAATTAAATCCTGAGTTATATGTTAATATG
CCAGGAGTAATTAAGGTATTAGGTAATCAGGATAAACTTAAATATGAGAGTTATCTTGCTAAGGATAAAATTATTGTAAG
TAAGATAGAGAATTCAAAAGAAGTTGTGCAAGATTCAGTTAAAGGATTTTCTGGCAAATCTAGAGCATTAATTGAAATTC
AAAATGGATGTAATCATGAATGTACTTTTTGTGTAATTACAAAGGCAAGAGGTAATAACCGTTCTCTATATATAGAAGAC
ATTATCACTAAAGTTAAAGCTTGTGTTGATAATGGGTATAATGAAGTTGTGTTTACTGGGGTAGATATAAGTGATTTTGG
ATTAGATATTTATGGACAACGTGTTCTTGGGACAATGATAAAAAGAGTATTGAGTGCCGTTCCACAGTTGCGTAGATTGA
GATTATCATCCATAGATGTAGCAGAAATAGAGGATGATCTTATTAATATTATTATTAATGAGCCTAGATTTATGCCACAC
TTACATCTAAGCTTACAATCAGGAAATAATTTAATCCTTAAAAGAATGAAGCGTAGACATAGTAGAGAGCAGGTAATAGA
GTTTTGTAATAAAATAACTAGTAAAAGGAAAGAAGTAGTATTTGGGGCTGATATTATAGTAGGATTCCCTACGGAAACTG
AAGAGATGTTTAATGATACAGTGAGATTGATAGAAGAAGCAAATATTTCTTATCTACATGTTTTTCCATATTCAAAGAGA
GAAGGTACCCCAGCTGCTAGAATGCCACAAGTTGTACAAGAAGTGAAGAAAAGACGTGTAAAACACTTATTAGAGTTTGC
TGAGAAAAGATTGCATGATTTTTATAGTACTCTGTTACATACAAAGCAAAGTGTTGTAGTTGAAAAGTCAGGAACTGGTA
GAGCAGAAAATTTTGCTTTGGTGAAATTTTTGGATGATAATGTTCAATTGCAAAGTATTGTTGAAGTGGAAATAAAAAGA
GTAGAAGGCAATTATTTAGTAGGTCAGGTATTTGTTTAG

Upstream 100 bases:

>100_bases
AAATAGCAATTAATACGTATTTGTTAGTTGTCTTGATTTTTAATTAGAAGATATCATAGTTATATAGGGTATGCTGGTTG
ATATGAGTTAGAGAGTCGTA

Downstream 100 bases:

>100_bases
TAATGTCTAATTTTAAAACTAAAGCTTCTAAATGTATACTGTGCTAATTCTTTTTTTGTGTGTCTATAAAAATAAAACTT
AATTTTATTGTGATGAAGCT

Product: MiaB family tRNA modification protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSDVITFGCRLNFYESEVIKNNLKKAQLDDVIVVHSCAVTNEAERQVRSRIRKLYRDNANVKIIVAGCAVQLNPELYVNM
PGVIKVLGNQDKLKYESYLAKDKIIVSKIENSKEVVQDSVKGFSGKSRALIEIQNGCNHECTFCVITKARGNNRSLYIED
IITKVKACVDNGYNEVVFTGVDISDFGLDIYGQRVLGTMIKRVLSAVPQLRRLRLSSIDVAEIEDDLINIIINEPRFMPH
LHLSLQSGNNLILKRMKRRHSREQVIEFCNKITSKRKEVVFGADIIVGFPTETEEMFNDTVRLIEEANISYLHVFPYSKR
EGTPAARMPQVVQEVKKRRVKHLLEFAEKRLHDFYSTLLHTKQSVVVEKSGTGRAENFALVKFLDDNVQLQSIVEVEIKR
VEGNYLVGQVFV

Sequences:

>Translated_412_residues
MSDVITFGCRLNFYESEVIKNNLKKAQLDDVIVVHSCAVTNEAERQVRSRIRKLYRDNANVKIIVAGCAVQLNPELYVNM
PGVIKVLGNQDKLKYESYLAKDKIIVSKIENSKEVVQDSVKGFSGKSRALIEIQNGCNHECTFCVITKARGNNRSLYIED
IITKVKACVDNGYNEVVFTGVDISDFGLDIYGQRVLGTMIKRVLSAVPQLRRLRLSSIDVAEIEDDLINIIINEPRFMPH
LHLSLQSGNNLILKRMKRRHSREQVIEFCNKITSKRKEVVFGADIIVGFPTETEEMFNDTVRLIEEANISYLHVFPYSKR
EGTPAARMPQVVQEVKKRRVKHLLEFAEKRLHDFYSTLLHTKQSVVVEKSGTGRAENFALVKFLDDNVQLQSIVEVEIKR
VEGNYLVGQVFV
>Mature_411_residues
SDVITFGCRLNFYESEVIKNNLKKAQLDDVIVVHSCAVTNEAERQVRSRIRKLYRDNANVKIIVAGCAVQLNPELYVNMP
GVIKVLGNQDKLKYESYLAKDKIIVSKIENSKEVVQDSVKGFSGKSRALIEIQNGCNHECTFCVITKARGNNRSLYIEDI
ITKVKACVDNGYNEVVFTGVDISDFGLDIYGQRVLGTMIKRVLSAVPQLRRLRLSSIDVAEIEDDLINIIINEPRFMPHL
HLSLQSGNNLILKRMKRRHSREQVIEFCNKITSKRKEVVFGADIIVGFPTETEEMFNDTVRLIEEANISYLHVFPYSKRE
GTPAARMPQVVQEVKKRRVKHLLEFAEKRLHDFYSTLLHTKQSVVVEKSGTGRAENFALVKFLDDNVQLQSIVEVEIKRV
EGNYLVGQVFV

Specific function: Unknown

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MTTase N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=449, Percent_Identity=28.7305122494432, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI28872784, Length=457, Percent_Identity=24.72647702407, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI28872782, Length=457, Percent_Identity=24.72647702407, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1786882, Length=443, Percent_Identity=27.313769751693, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1787057, Length=367, Percent_Identity=28.0653950953678, Blast_Score=131, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17553146, Length=419, Percent_Identity=24.8210023866348, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71996771, Length=213, Percent_Identity=30.0469483568075, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21356207, Length=424, Percent_Identity=27.122641509434, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19922432, Length=390, Percent_Identity=26.6666666666667, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006467
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 46951; Mature: 46820

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVITFGCRLNFYESEVIKNNLKKAQLDDVIVVHSCAVTNEAERQVRSRIRKLYRDNAN
CCCEEEEEEEEEHHHHHHHHHCCCHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
VKIIVAGCAVQLNPELYVNMPGVIKVLGNQDKLKYESYLAKDKIIVSKIENSKEVVQDSV
EEEEEEEEEEEECCEEEEECCHHEEEECCCCCHHHHHHHHHCHHEEHHHCCHHHHHHHHH
KGFSGKSRALIEIQNGCNHECTFCVITKARGNNRSLYIEDIITKVKACVDNGYNEVVFTG
HCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEE
VDISDFGLDIYGQRVLGTMIKRVLSAVPQLRRLRLSSIDVAEIEDDLINIIINEPRFMPH
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCCCE
LHLSLQSGNNLILKRMKRRHSREQVIEFCNKITSKRKEVVFGADIIVGFPTETEEMFNDT
EEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEECEEEEECCCCHHHHHHHH
VRLIEEANISYLHVFPYSKREGTPAARMPQVVQEVKKRRVKHLLEFAEKRLHDFYSTLLH
HHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKQSVVVEKSGTGRAENFALVKFLDDNVQLQSIVEVEIKRVEGNYLVGQVFV
CCHHEEEECCCCCCCCCEEEEEEECCCCHHHHHHEEHHEEECCCEEEEEEEC
>Mature Secondary Structure 
SDVITFGCRLNFYESEVIKNNLKKAQLDDVIVVHSCAVTNEAERQVRSRIRKLYRDNAN
CCEEEEEEEEEHHHHHHHHHCCCHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
VKIIVAGCAVQLNPELYVNMPGVIKVLGNQDKLKYESYLAKDKIIVSKIENSKEVVQDSV
EEEEEEEEEEEECCEEEEECCHHEEEECCCCCHHHHHHHHHCHHEEHHHCCHHHHHHHHH
KGFSGKSRALIEIQNGCNHECTFCVITKARGNNRSLYIEDIITKVKACVDNGYNEVVFTG
HCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEE
VDISDFGLDIYGQRVLGTMIKRVLSAVPQLRRLRLSSIDVAEIEDDLINIIINEPRFMPH
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCCCE
LHLSLQSGNNLILKRMKRRHSREQVIEFCNKITSKRKEVVFGADIIVGFPTETEEMFNDT
EEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEEECEEEEECCCCHHHHHHHH
VRLIEEANISYLHVFPYSKREGTPAARMPQVVQEVKKRRVKHLLEFAEKRLHDFYSTLLH
HHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKQSVVVEKSGTGRAENFALVKFLDDNVQLQSIVEVEIKRVEGNYLVGQVFV
CCHHEEEECCCCCCCCCEEEEEEECCCCHHHHHHEEHHEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]