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The map label for this gene is dnaA

Identifier: 88658026

GI number: 88658026

Start: 822485

End: 823879

Strand: Reverse

Name: dnaA

Synonym: ECH_0809

Alternate gene names: 88658026

Gene position: 823879-822485 (Counterclockwise)

Preceding gene: 88658534

Following gene: 88658591

Centisome position: 70.04

GC content: 32.04

Gene sequence:

>1395_bases
ATGAATGATGGTGGTACTGGGCTTTTAGGAGATTGTGTAAAAGAAAAGCTTCAATCTGACAACCAGGTAATTTGGAAAGA
TATTCAAGTCAAATTACATAAGTTTTATGGTAATGCCATATATGATAGTTGGTTGAGTGTACTAGTATATATTAATAATG
AAGGTGGTAAGGTTTTACTGTCTGCTCCAACTAGGTTTATCAAAGAATGGATATTAGTGCATTACTTGGATCAAATTTTA
AAATATTGGCAGGATGAAGATCAAAGTGTATGTTCTGTAGACATTTGTGTTGTGAATAATCAAGATACTAATTTGCTAGT
GAATATCAAAGATCAGCAAGATAAGGAAGTAAGAAATAATTATGATCATTTAAGTTCTCCTTTGGATCCTAGATTTACTT
TTGATAATTTTGTAGTAGGAAAACCAAATGAGCTTGCTTTTGCTGCAGCTAGAAGAGTGGCAGAGTCTAATGAACCAATA
TCTGGGAGTAATCCTTTATTTCTGTATGGTGGAGTTGGACTTGGGAAAACTCATTTGATGCATGCTATTGCTTGGTATAT
TATAAAATCTTCTTCGAAAAGAAAAATAGCTTATCTTTCAGCAGAAAAATTTATGTATCAATATGTTACAGCATTAAGAA
GCAAGGATATAATGTTGTTTAAAGAGCAATTTCGTTCTGTAGACATATTGATGGTTGATGATGTTCAGTTTATTAGTGGT
AAAGACAGTACTCAGGAGGAATTCTTTCATACTTTTAATGCTTTAATAGATCAGAATAAGCAGTTAGTGATATCTGCTGA
TAGATCACCTAGTGATTTAGATGGAGTTGAAGAAAGGATAAAATCAAGATTAGGTTGGGGATTAGTCGCAGATATTAATG
AAACAACTTTTGAGTTACGGTTAGGTATATTGCAGCTAAAAGTTGAAAAGATGGGTATTAATATTCCAAATAAAGTATTA
GAGTTTTTAGCAAAGAATATCAAATCCAATATTAGAGAATTAGAGGGTGCGCTTAACAAAGTAGTTGCACATTCTTCATT
AGTTGGATGTAGTATTACTTTGGATTCAGCTAGTGATATTTTATCTGATTTATTGCGTGCAAATCACAAATCAGTGACAT
TAGAATGTATTCAAAGGAAAGTTGCTGAATTCTTTAATATTAAGATAACTGACATGTACTCTACTAGAAGGTTAAGAACT
TTAGCGCGTCCTAGGCAAGTAGCAATGTATCTATCAAAGAAATTGACTCAGAAGAGTTTACCAGAAATTGGAAAAAGTTT
TGGGGGTAGAGATCATGCTACAGTAATTCATGCTGTTAAGCAGATAGAAAAATTAATAGATACAGACAGTAAACTGAGAG
ATGATATTAATTTATTGACTAGAATGTTGAGATAA

Upstream 100 bases:

>100_bases
GTTTTTCTATTGTATAATGCAGAGGATCGTAACTCTATATATAGTGATGCTATAAGATTTATGATTTTTCAAGTTATGAT
TGTAGTTTTGGATTGGCTAT

Downstream 100 bases:

>100_bases
ATGATACCTGAATAAGCATACTGTATTGAGAATTAGAATGTATTCAAAGGAAAGTTGCTGAGTTCTTTAAATTAAGACAA
CTGACATGTACTCTACTAGA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MNDGGTGLLGDCVKEKLQSDNQVIWKDIQVKLHKFYGNAIYDSWLSVLVYINNEGGKVLLSAPTRFIKEWILVHYLDQIL
KYWQDEDQSVCSVDICVVNNQDTNLLVNIKDQQDKEVRNNYDHLSSPLDPRFTFDNFVVGKPNELAFAAARRVAESNEPI
SGSNPLFLYGGVGLGKTHLMHAIAWYIIKSSSKRKIAYLSAEKFMYQYVTALRSKDIMLFKEQFRSVDILMVDDVQFISG
KDSTQEEFFHTFNALIDQNKQLVISADRSPSDLDGVEERIKSRLGWGLVADINETTFELRLGILQLKVEKMGINIPNKVL
EFLAKNIKSNIRELEGALNKVVAHSSLVGCSITLDSASDILSDLLRANHKSVTLECIQRKVAEFFNIKITDMYSTRRLRT
LARPRQVAMYLSKKLTQKSLPEIGKSFGGRDHATVIHAVKQIEKLIDTDSKLRDDINLLTRMLR

Sequences:

>Translated_464_residues
MNDGGTGLLGDCVKEKLQSDNQVIWKDIQVKLHKFYGNAIYDSWLSVLVYINNEGGKVLLSAPTRFIKEWILVHYLDQIL
KYWQDEDQSVCSVDICVVNNQDTNLLVNIKDQQDKEVRNNYDHLSSPLDPRFTFDNFVVGKPNELAFAAARRVAESNEPI
SGSNPLFLYGGVGLGKTHLMHAIAWYIIKSSSKRKIAYLSAEKFMYQYVTALRSKDIMLFKEQFRSVDILMVDDVQFISG
KDSTQEEFFHTFNALIDQNKQLVISADRSPSDLDGVEERIKSRLGWGLVADINETTFELRLGILQLKVEKMGINIPNKVL
EFLAKNIKSNIRELEGALNKVVAHSSLVGCSITLDSASDILSDLLRANHKSVTLECIQRKVAEFFNIKITDMYSTRRLRT
LARPRQVAMYLSKKLTQKSLPEIGKSFGGRDHATVIHAVKQIEKLIDTDSKLRDDINLLTRMLR
>Mature_464_residues
MNDGGTGLLGDCVKEKLQSDNQVIWKDIQVKLHKFYGNAIYDSWLSVLVYINNEGGKVLLSAPTRFIKEWILVHYLDQIL
KYWQDEDQSVCSVDICVVNNQDTNLLVNIKDQQDKEVRNNYDHLSSPLDPRFTFDNFVVGKPNELAFAAARRVAESNEPI
SGSNPLFLYGGVGLGKTHLMHAIAWYIIKSSSKRKIAYLSAEKFMYQYVTALRSKDIMLFKEQFRSVDILMVDDVQFISG
KDSTQEEFFHTFNALIDQNKQLVISADRSPSDLDGVEERIKSRLGWGLVADINETTFELRLGILQLKVEKMGINIPNKVL
EFLAKNIKSNIRELEGALNKVVAHSSLVGCSITLDSASDILSDLLRANHKSVTLECIQRKVAEFFNIKITDMYSTRRLRT
LARPRQVAMYLSKKLTQKSLPEIGKSFGGRDHATVIHAVKQIEKLIDTDSKLRDDINLLTRMLR

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=428, Percent_Identity=42.5233644859813, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI226510964, Length=230, Percent_Identity=23.9130434782609, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_EHRCR (Q2GG27)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507606.1
- ProteinModelPortal:   Q2GG27
- STRING:   Q2GG27
- GeneID:   3927615
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0809
- TIGR:   ECH_0809
- eggNOG:   COG0593
- HOGENOM:   HBG695666
- OMA:   CHTLNAL
- PhylomeDB:   Q2GG27
- ProtClustDB:   PRK00149
- BioCyc:   ECHA205920:ECH_0809-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 52768; Mature: 52768

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDGGTGLLGDCVKEKLQSDNQVIWKDIQVKLHKFYGNAIYDSWLSVLVYINNEGGKVLL
CCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCHHHHHHHHHEEEEEECCCCEEEE
SAPTRFIKEWILVHYLDQILKYWQDEDQSVCSVDICVVNNQDTNLLVNIKDQQDKEVRNN
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHHC
YDHLSSPLDPRFTFDNFVVGKPNELAFAAARRVAESNEPISGSNPLFLYGGVGLGKTHLM
HHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHH
HAIAWYIIKSSSKRKIAYLSAEKFMYQYVTALRSKDIMLFKEQFRSVDILMVDDVQFISG
HHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEECCCCEECC
KDSTQEEFFHTFNALIDQNKQLVISADRSPSDLDGVEERIKSRLGWGLVADINETTFELR
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHH
LGILQLKVEKMGINIPNKVLEFLAKNIKSNIRELEGALNKVVAHSSLVGCSITLDSASDI
HHHHHEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHH
LSDLLRANHKSVTLECIQRKVAEFFNIKITDMYSTRRLRTLARPRQVAMYLSKKLTQKSL
HHHHHHCCCCHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PEIGKSFGGRDHATVIHAVKQIEKLIDTDSKLRDDINLLTRMLR
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNDGGTGLLGDCVKEKLQSDNQVIWKDIQVKLHKFYGNAIYDSWLSVLVYINNEGGKVLL
CCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCHHHHHHHHHEEEEEECCCCEEEE
SAPTRFIKEWILVHYLDQILKYWQDEDQSVCSVDICVVNNQDTNLLVNIKDQQDKEVRNN
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHHC
YDHLSSPLDPRFTFDNFVVGKPNELAFAAARRVAESNEPISGSNPLFLYGGVGLGKTHLM
HHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHH
HAIAWYIIKSSSKRKIAYLSAEKFMYQYVTALRSKDIMLFKEQFRSVDILMVDDVQFISG
HHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEECCCCEECC
KDSTQEEFFHTFNALIDQNKQLVISADRSPSDLDGVEERIKSRLGWGLVADINETTFELR
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEECCCCHHHHHH
LGILQLKVEKMGINIPNKVLEFLAKNIKSNIRELEGALNKVVAHSSLVGCSITLDSASDI
HHHHHEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHH
LSDLLRANHKSVTLECIQRKVAEFFNIKITDMYSTRRLRTLARPRQVAMYLSKKLTQKSL
HHHHHHCCCCHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PEIGKSFGGRDHATVIHAVKQIEKLIDTDSKLRDDINLLTRMLR
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA