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The map label for this gene is gshB [H]

Identifier: 88658015

GI number: 88658015

Start: 325798

End: 326745

Strand: Direct

Name: gshB [H]

Synonym: ECH_0336

Alternate gene names: 88658015

Gene position: 325798-326745 (Clockwise)

Preceding gene: 88658506

Following gene: 88657809

Centisome position: 27.7

GC content: 30.59

Gene sequence:

>948_bases
ATGGCACTAATTGTTGCTTTTCAAATGGATAAAGATGTTGTTGTTGATAAGGATATGTCAGTTAAATTTATACAAGAAGC
GCAAAATAGGGGGCATACTGTATTCTTTTATTCTCCTAATCAATTAGCAGTAAAAGATAACATGCCAATTGCCAGAGCAT
GCTTGGTAACTATAGAAGGTCAAGAACTACGATTTGCTGATGTACAAGAATTGTTGCTTGAAGATGTTAATATAATTTTT
GTGCGTCAAAACCCACCATTTGACATGCGTTATATTACAAGTACATATATTTTAGAAAGATTAAAAAGACCACTTGTGAT
AAATAACCCTAAAAGTCTTAGAGATCATACAGAAAAATTATTACCACTTTCATTCCCTGAATTTATACCAGATACTATAG
TTACTGAAGATTATATTATGTTGCAGGATTTCTATAATAAACATAAGGATATAGTGTTAAAGCCTTTATATAGTTATGGT
GGAAATGATGTTACTAGAATGCATAGTAATGTTGATATTGGGGTTATAGCGAAATTAATGACAGAAAAATATGGATGCCC
TATAATTGCTCAAAAATTTATACCAAATGTCAGTAACGATAAGAGAATTATTCTTTTGGATGGTAAGCCTATAGGAGTTT
TTAAACGTAGAATATCATTTAATGGAATTAATGAAGAAATAAGAACTAATTTGCGAATGGGATCAGTTCCAGAAGTTGCA
ACATTGTCAGATCGAGATATAGCTATATGTTCAGCTATAGAACAAGAGTTGTTGGGCAAAGGAATGGTATTTGTTGGAAT
TGATGTGTTAGATGGGTATTTGATAGAAATAAATGTTACTTCTCCATGTGGTATTTTACAAATTAATGAGTTGTATGGTG
TGTCTTTAGAAAAAAAATGTTGGGATTGTTTTGAAGAGAAGTTTTATGAAAAACATAATAGTATATAG

Upstream 100 bases:

>100_bases
CTTTTATATTTCGATATCTTAAGAATTTTGTTATTCTCATTCAATAAAGTAAGGGTTATAAGAAGCTATCGGCTTAGTAG
AGAAATATAAGAGGTAATAT

Downstream 100 bases:

>100_bases
ATTATGCTGTTTGTAAAAACTAGTAACTTTAATAGTGCTTCTATTATTTGAATTGAATATAATAACGTATTAATAAAAAT
TTTCTGACTATAGGGATAAA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MALIVAFQMDKDVVVDKDMSVKFIQEAQNRGHTVFFYSPNQLAVKDNMPIARACLVTIEGQELRFADVQELLLEDVNIIF
VRQNPPFDMRYITSTYILERLKRPLVINNPKSLRDHTEKLLPLSFPEFIPDTIVTEDYIMLQDFYNKHKDIVLKPLYSYG
GNDVTRMHSNVDIGVIAKLMTEKYGCPIIAQKFIPNVSNDKRIILLDGKPIGVFKRRISFNGINEEIRTNLRMGSVPEVA
TLSDRDIAICSAIEQELLGKGMVFVGIDVLDGYLIEINVTSPCGILQINELYGVSLEKKCWDCFEEKFYEKHNSI

Sequences:

>Translated_315_residues
MALIVAFQMDKDVVVDKDMSVKFIQEAQNRGHTVFFYSPNQLAVKDNMPIARACLVTIEGQELRFADVQELLLEDVNIIF
VRQNPPFDMRYITSTYILERLKRPLVINNPKSLRDHTEKLLPLSFPEFIPDTIVTEDYIMLQDFYNKHKDIVLKPLYSYG
GNDVTRMHSNVDIGVIAKLMTEKYGCPIIAQKFIPNVSNDKRIILLDGKPIGVFKRRISFNGINEEIRTNLRMGSVPEVA
TLSDRDIAICSAIEQELLGKGMVFVGIDVLDGYLIEINVTSPCGILQINELYGVSLEKKCWDCFEEKFYEKHNSI
>Mature_314_residues
ALIVAFQMDKDVVVDKDMSVKFIQEAQNRGHTVFFYSPNQLAVKDNMPIARACLVTIEGQELRFADVQELLLEDVNIIFV
RQNPPFDMRYITSTYILERLKRPLVINNPKSLRDHTEKLLPLSFPEFIPDTIVTEDYIMLQDFYNKHKDIVLKPLYSYGG
NDVTRMHSNVDIGVIAKLMTEKYGCPIIAQKFIPNVSNDKRIILLDGKPIGVFKRRISFNGINEEIRTNLRMGSVPEVAT
LSDRDIAICSAIEQELLGKGMVFVGIDVLDGYLIEINVTSPCGILQINELYGVSLEKKCWDCFEEKFYEKHNSI

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=309, Percent_Identity=35.2750809061489, Blast_Score=178, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 36047; Mature: 35916

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVAFQMDKDVVVDKDMSVKFIQEAQNRGHTVFFYSPNQLAVKDNMPIARACLVTIEG
CEEEEEEECCCCEEECCCCCHHHHHHHHHCCCEEEEECCCEEEEECCCCEEEEEEEEECC
QELRFADVQELLLEDVNIIFVRQNPPFDMRYITSTYILERLKRPLVINNPKSLRDHTEKL
CCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHC
LPLSFPEFIPDTIVTEDYIMLQDFYNKHKDIVLKPLYSYGGNDVTRMHSNVDIGVIAKLM
CCCCCHHHCCHHHCCCCHHHHHHHHCCCCCEEEEEHHHCCCCCEEEEECCCCHHHHHHHH
TEKYGCPIIAQKFIPNVSNDKRIILLDGKPIGVFKRRISFNGINEEIRTNLRMGSVPEVA
HHHCCCCCHHHHHCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCEE
TLSDRDIAICSAIEQELLGKGMVFVGIDVLDGYLIEINVTSPCGILQINELYGVSLEKKC
ECCCCCHHHHHHHHHHHHHCCEEEEEEEECCCEEEEEEECCCCCEEEEHHHHCCCHHHHH
WDCFEEKFYEKHNSI
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALIVAFQMDKDVVVDKDMSVKFIQEAQNRGHTVFFYSPNQLAVKDNMPIARACLVTIEG
EEEEEEECCCCEEECCCCCHHHHHHHHHCCCEEEEECCCEEEEECCCCEEEEEEEEECC
QELRFADVQELLLEDVNIIFVRQNPPFDMRYITSTYILERLKRPLVINNPKSLRDHTEKL
CCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHC
LPLSFPEFIPDTIVTEDYIMLQDFYNKHKDIVLKPLYSYGGNDVTRMHSNVDIGVIAKLM
CCCCCHHHCCHHHCCCCHHHHHHHHCCCCCEEEEEHHHCCCCCEEEEECCCCHHHHHHHH
TEKYGCPIIAQKFIPNVSNDKRIILLDGKPIGVFKRRISFNGINEEIRTNLRMGSVPEVA
HHHCCCCCHHHHHCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHCCCCCCCCCEE
TLSDRDIAICSAIEQELLGKGMVFVGIDVLDGYLIEINVTSPCGILQINELYGVSLEKKC
ECCCCCHHHHHHHHHHHHHCCEEEEEEEECCCEEEEEEECCCCCEEEEHHHHCCCHHHHH
WDCFEEKFYEKHNSI
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1540152 [H]