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The map label for this gene is nth [C]

Identifier: 88657996

GI number: 88657996

Start: 877542

End: 877679

Strand: Reverse

Name: nth [C]

Synonym: ECH_0856

Alternate gene names: 88657996

Gene position: 877679-877542 (Counterclockwise)

Preceding gene: 88658370

Following gene: 88657889

Centisome position: 74.62

GC content: 31.88

Gene sequence:

>138_bases
ATGAACTTGCTAGAGTGTGATGTTAAGTGTATTACTTGGTTAATATTGCATGATAGACATGTGTGTAAGTCAAGAAAGCC
ATTGTGTAGTCAGTGTGTTGTTCAAGATTTATGTGAATATGAAAGTAAAAGTTTATGA

Upstream 100 bases:

>100_bases
ATTGGTTAGTATTACATGGTAGGTATGTGTGTAAGTCAAGAAAGCCATTGTGTAGTCAGTGTGTTGTTCAAGATTTATGT
GAATATGAAAGTAAAAGTTT

Downstream 100 bases:

>100_bases
ACTTGCTAGAGTGTGATGTTAAGTGTATTATTTGATTAATATTGCATGATAGATATGTGTGTAAGTCAAGAAAGCCGTTG
TGTAGTTAGTGTGTTGTTCA

Product: endonuclease III

Products: 3'-terminal 1-oxo-4,5-dihydroxy-2-pentene; 5'-terminal cyclobutadipyrimidine deoxyribose 5'-phosphate DNA [C]

Alternate protein names: NA

Number of amino acids: Translated: 45; Mature: 45

Protein sequence:

>45_residues
MNLLECDVKCITWLILHDRHVCKSRKPLCSQCVVQDLCEYESKSL

Sequences:

>Translated_45_residues
MNLLECDVKCITWLILHDRHVCKSRKPLCSQCVVQDLCEYESKSL
>Mature_45_residues
MNLLECDVKCITWLILHDRHVCKSRKPLCSQCVVQDLCEYESKSL

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.99.18 [C]

Molecular weight: Translated: 5281; Mature: 5281

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

13.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
15.6 %Cys+Met (Translated Protein)
13.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
15.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLECDVKCITWLILHDRHVCKSRKPLCSQCVVQDLCEYESKSL
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLLECDVKCITWLILHDRHVCKSRKPLCSQCVVQDLCEYESKSL
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyclobutadipyrimidine (cross-linked pyrimidine dimer) in DNA; apyrimidinic phosphodiester; DNA [C]

Specific reaction: Endonucleolytic Cleavage Near Apurinic Or Apyrimidinic Sites To Products With 5'-Phosphate Protein + DNA = Protein-DNA [C]

General reaction: Carbon-Oxygen Lyase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA