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The map label for this gene is glyA

Identifier: 88657955

GI number: 88657955

Start: 297856

End: 299118

Strand: Direct

Name: glyA

Synonym: ECH_0311

Alternate gene names: 88657955

Gene position: 297856-299118 (Clockwise)

Preceding gene: 88658574

Following gene: 88657643

Centisome position: 25.32

GC content: 35.0

Gene sequence:

>1263_bases
ATGGTTGGGTATATCTTAGATGATGATTTACAGGAAGTTGATGCTGAGGTTTTTAATTGTATTTCTGGAGAGTTAAATCG
ACAAAATTCTGGACTACAGTTAATAGCGTCAGAAAATTTTGTGAGTAAGGCAGTATTGCAAGCTCAGGGTTCTATATTTA
CAAATAAGTATGCTGAAGGTTATCCAGGAAAAAGATATTATTGTGGATGTCATTTTGCAGATATTGTAGAAAATTTGGCA
ATAGAACGTTTATGTCGATTGTTTGGATGTAAATTTGCAAATGTTCAACCGCATTCTGGTTCACAAGCTAATCAAGGAGT
ATTTGCTGCATTGTTAAAGCCAGGTGACACTGTCATTGGTATGTCACTTGATTGTGGTGGTCATTTAACTCATGGTTCTG
CTCCTAGTATTTCTGGAAAGTGGTTTAATGCTGTGCAGTATCAAGTTGATCGTGATACTGGTTTAATAGATATGGATGAA
ATAGAGAAGTTAGCTGTTGAACATAATCCATCATTAATTATTGCTGGTTCATCTTCTTATCCTCGTGTAATAGATTTTAA
AAGGTTTAGGGAAATAGCTGATAAAGTGGGAGCATATCTTCTAGCTGATATAGCGCATTATGCTGGGTTAATTGCTGCTG
GAGAATTTCCTTCGCCTGTTGAATATGCTCATGTTATTACGTCTACTACTCACAAGACATTGCGTGGCCCTAGGGGAGCA
GTGATAATGACTAATTATGAAGATATCCATAAGAAAATTCAATCTTCTATTTTTCCTGGTATGCAGGGTGGTCCGTTAAT
GCATGTTATTGCTGCTAAAGCTGTAGCATTTGCAGAAGCATTGAAACCTGAGTTTAAAGATTATGCGAAACAAATAATAA
AAAATTCTAAGGCTTTGGGTGAAGTATTTAAAGAAAGAGGGCTTGATCTTGTGACAGGTGGTACAGATTCTCATATGGTT
GTTCTTGATTTACGTTCAAAAAGTGTTACAGGTAAAGATGCAGTTCTTGCTTTAGAAAAACTAGGAATTATTTGTAATAA
AAATGCTATTCCTTTTGATCCAGAAAAGCCTTTTGTAACTTCTGGTTTACGTTTTGGTAGTGCTGCTGAGACTTCCAGAG
GGTTACAAGAATCAGAATTTAGGGAAATTGGCAGTATGGTTTGTGATGTTATTGATAGTTTAAAAGCTAGTGATAGTGTT
AGATTATCTGTTGAAAGAGATATAATAAAAAGAGTTAAGGAATTAACTTCTAATTTTATTTAG

Upstream 100 bases:

>100_bases
AGTGAGAAACAGGATAGCGTTTCTGAATAACACTTTAAGTTGTATCTAATTGTAATAGCGCTGTTAAGAGTTTTTTATAA
TTTATTACGGGGTTCTTTCT

Downstream 100 bases:

>100_bases
TTAAGTCAATGTAAAATGATATTTAAAAATGTAAATAATTTAGTTTTTATTTACATACTTTTTTAAATGTGATTAACATA
TAATTAGCTATATTTTTGAT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MVGYILDDDLQEVDAEVFNCISGELNRQNSGLQLIASENFVSKAVLQAQGSIFTNKYAEGYPGKRYYCGCHFADIVENLA
IERLCRLFGCKFANVQPHSGSQANQGVFAALLKPGDTVIGMSLDCGGHLTHGSAPSISGKWFNAVQYQVDRDTGLIDMDE
IEKLAVEHNPSLIIAGSSSYPRVIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPVEYAHVITSTTHKTLRGPRGA
VIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFAEALKPEFKDYAKQIIKNSKALGEVFKERGLDLVTGGTDSHMV
VLDLRSKSVTGKDAVLALEKLGIICNKNAIPFDPEKPFVTSGLRFGSAAETSRGLQESEFREIGSMVCDVIDSLKASDSV
RLSVERDIIKRVKELTSNFI

Sequences:

>Translated_420_residues
MVGYILDDDLQEVDAEVFNCISGELNRQNSGLQLIASENFVSKAVLQAQGSIFTNKYAEGYPGKRYYCGCHFADIVENLA
IERLCRLFGCKFANVQPHSGSQANQGVFAALLKPGDTVIGMSLDCGGHLTHGSAPSISGKWFNAVQYQVDRDTGLIDMDE
IEKLAVEHNPSLIIAGSSSYPRVIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPVEYAHVITSTTHKTLRGPRGA
VIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFAEALKPEFKDYAKQIIKNSKALGEVFKERGLDLVTGGTDSHMV
VLDLRSKSVTGKDAVLALEKLGIICNKNAIPFDPEKPFVTSGLRFGSAAETSRGLQESEFREIGSMVCDVIDSLKASDSV
RLSVERDIIKRVKELTSNFI
>Mature_420_residues
MVGYILDDDLQEVDAEVFNCISGELNRQNSGLQLIASENFVSKAVLQAQGSIFTNKYAEGYPGKRYYCGCHFADIVENLA
IERLCRLFGCKFANVQPHSGSQANQGVFAALLKPGDTVIGMSLDCGGHLTHGSAPSISGKWFNAVQYQVDRDTGLIDMDE
IEKLAVEHNPSLIIAGSSSYPRVIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPVEYAHVITSTTHKTLRGPRGA
VIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFAEALKPEFKDYAKQIIKNSKALGEVFKERGLDLVTGGTDSHMV
VLDLRSKSVTGKDAVLALEKLGIICNKNAIPFDPEKPFVTSGLRFGSAAETSRGLQESEFREIGSMVCDVIDSLKASDSV
RLSVERDIIKRVKELTSNFI

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=419, Percent_Identity=43.6754176610979, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI261862350, Length=419, Percent_Identity=43.6754176610979, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI261862348, Length=419, Percent_Identity=43.6754176610979, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI19923315, Length=419, Percent_Identity=43.6754176610979, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI22547186, Length=412, Percent_Identity=46.1165048543689, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI261862346, Length=419, Percent_Identity=42.4821002386635, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI22547189, Length=395, Percent_Identity=44.5569620253165, Blast_Score=295, Evalue=4e-80,
Organism=Escherichia coli, GI1788902, Length=407, Percent_Identity=54.5454545454545, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI25144729, Length=454, Percent_Identity=43.8325991189427, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI25144732, Length=454, Percent_Identity=43.8325991189427, Blast_Score=352, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6323087, Length=411, Percent_Identity=45.0121654501217, Blast_Score=339, Evalue=6e-94,
Organism=Saccharomyces cerevisiae, GI6319739, Length=435, Percent_Identity=41.3793103448276, Blast_Score=331, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24640005, Length=408, Percent_Identity=44.3627450980392, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI221329721, Length=408, Percent_Identity=44.3627450980392, Blast_Score=342, Evalue=2e-94,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_EHRCR (Q2GHF1)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507132.1
- HSSP:   P34897
- ProteinModelPortal:   Q2GHF1
- SMR:   Q2GHF1
- STRING:   Q2GHF1
- GeneID:   3927190
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0311
- TIGR:   ECH_0311
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   DYDQIAA
- PhylomeDB:   Q2GHF1
- ProtClustDB:   PRK00011
- BioCyc:   ECHA205920:ECH_0311-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45758; Mature: 45758

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGYILDDDLQEVDAEVFNCISGELNRQNSGLQLIASENFVSKAVLQAQGSIFTNKYAEG
CCCCEECCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHCCCCCCC
YPGKRYYCGCHFADIVENLAIERLCRLFGCKFANVQPHSGSQANQGVFAALLKPGDTVIG
CCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
MSLDCGGHLTHGSAPSISGKWFNAVQYQVDRDTGLIDMDEIEKLAVEHNPSLIIAGSSSY
EEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCC
PRVIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPVEYAHVITSTTHKTLRGPRGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCCE
VIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFAEALKPEFKDYAKQIIKNSKALG
EEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHH
EVFKERGLDLVTGGTDSHMVVLDLRSKSVTGKDAVLALEKLGIICNKNAIPFDPEKPFVT
HHHHHCCCEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCEECCCCCCCCCCCCHHH
SGLRFGSAAETSRGLQESEFREIGSMVCDVIDSLKASDSVRLSVERDIIKRVKELTSNFI
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVGYILDDDLQEVDAEVFNCISGELNRQNSGLQLIASENFVSKAVLQAQGSIFTNKYAEG
CCCCEECCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCHHCCCCCCC
YPGKRYYCGCHFADIVENLAIERLCRLFGCKFANVQPHSGSQANQGVFAALLKPGDTVIG
CCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEE
MSLDCGGHLTHGSAPSISGKWFNAVQYQVDRDTGLIDMDEIEKLAVEHNPSLIIAGSSSY
EEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCC
PRVIDFKRFREIADKVGAYLLADIAHYAGLIAAGEFPSPVEYAHVITSTTHKTLRGPRGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHCCCCCE
VIMTNYEDIHKKIQSSIFPGMQGGPLMHVIAAKAVAFAEALKPEFKDYAKQIIKNSKALG
EEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHH
EVFKERGLDLVTGGTDSHMVVLDLRSKSVTGKDAVLALEKLGIICNKNAIPFDPEKPFVT
HHHHHCCCEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCEECCCCCCCCCCCCHHH
SGLRFGSAAETSRGLQESEFREIGSMVCDVIDSLKASDSVRLSVERDIIKRVKELTSNFI
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA