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The map label for this gene is ispG [H]

Identifier: 88657947

GI number: 88657947

Start: 562145

End: 563374

Strand: Direct

Name: ispG [H]

Synonym: ECH_0559

Alternate gene names: 88657947

Gene position: 562145-563374 (Clockwise)

Preceding gene: 88657823

Following gene: 88658564

Centisome position: 47.79

GC content: 33.66

Gene sequence:

>1230_bases
TTGTTTAATTGTATTGCAAATAAAAATAAAATAACCTATGAAGTGAAAGTTGGTGATGTAGTAATTGGTGGCAACAATCC
TGTAGTAGTGCAGTCAATGGCGTTAGGCGGATCTGGAGATGTGTATAAAGATGCACATGAGGTTTTAGAGTTAGCGCAAG
CTGGATCTGAATTGGTAAGAGTTGCAGTTAATTCAGAACAAGCTATGAAAAATGTTCCGTATATAAGAGATGTATTGGTA
GATCATGGCTTTAGTGCTAAGATGATAATAGGATGTGGACAATATGAAATTGCTAGATTGGTAAATGAGTATCCTGATTG
TGCAGCTGCTTTAGGAAAAATACGTATTAATCCAGGAAATGTTGGTTTTGGAAATAAACGGGATAAGAATTTTGAAGATA
TTGTTGAGTTTGCAATAAAACATGATATCCCTATCAGAATAGGTGTAAATTGGGGGAGTTTAGATAAGTATTTAGCTTCA
AAATTAATGAATGATAATGCATTACTTATCAACCCTAAGCCAGATTATATAGTTTTGCAGAAAGCATTGGTAATTTCTGC
TATAACAAGTGCTAAACGTGCAGAAGAAATTGGCTTATCTAAAAATAAGATAGTTATATCTTGTAAAACAAGTAAAATAC
AAGATTTAATACCTGTTTATACAGTATTGTCAAATGTATGTAATTATCCATTACATTTAGGGTTGACAGAAGCAGGGTCT
GGTACAAAAGGAATGGTTAGCAGTGCTGCAGGAATATCTTACTTATTGTTAAATGGTATAGGAGATACTATACGTGTTTC
CTTAACTCAACAACCTGGTGAAGCAAGAAGTATTGAAGTCAAGTTATGTCAAGAAATTTTGCAAAGTATAGGTTTAAGAA
ATTTTTCTGCGCAGGTAACTTCATGTCCAGGTTGTAATAGAACTAATCCTAAGTATTTTCACCAATTAGCTAAAGATATT
AATGATTATATAAAGCAACGTATGCCTGTGTGGAGAAATGATAATCCCGGATCTGAAAATATGACTGTAGCAGTAATGGG
TTGTATAGTCAATGGTCCAGGTGAAAGTAAACACGCAAATTTAGGTATTAGTCTTCCTGGCTATGGTGAGAGGCCTGTAG
CTGCAGTGTATCAGAATGGAGAGAAGTTGTGTACTTTAGAAGGCGGTAATATCTTTGAACAATTTGTATCAATTATCGAA
AATTATGTTAATGTTTATTACAAACAATAG

Upstream 100 bases:

>100_bases
TAGATTTGTTAGGGTTAACTGTATATAATTTTGCTTTGATACAAGGTTATGTTTTTGTATGCAATGTGAGATTGTAGATA
GAATTTTAAATGAAGATGGG

Downstream 100 bases:

>100_bases
TAAAAAAGGTAATCTATAATTATGGAATCTACAACTGGCGAAATGTTATTTTTTAAGCATTTTGAAGAATTGAGATATAG
AGTATTTTTTTGTTTTGCAT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MFNCIANKNKITYEVKVGDVVIGGNNPVVVQSMALGGSGDVYKDAHEVLELAQAGSELVRVAVNSEQAMKNVPYIRDVLV
DHGFSAKMIIGCGQYEIARLVNEYPDCAAALGKIRINPGNVGFGNKRDKNFEDIVEFAIKHDIPIRIGVNWGSLDKYLAS
KLMNDNALLINPKPDYIVLQKALVISAITSAKRAEEIGLSKNKIVISCKTSKIQDLIPVYTVLSNVCNYPLHLGLTEAGS
GTKGMVSSAAGISYLLLNGIGDTIRVSLTQQPGEARSIEVKLCQEILQSIGLRNFSAQVTSCPGCNRTNPKYFHQLAKDI
NDYIKQRMPVWRNDNPGSENMTVAVMGCIVNGPGESKHANLGISLPGYGERPVAAVYQNGEKLCTLEGGNIFEQFVSIIE
NYVNVYYKQ

Sequences:

>Translated_409_residues
MFNCIANKNKITYEVKVGDVVIGGNNPVVVQSMALGGSGDVYKDAHEVLELAQAGSELVRVAVNSEQAMKNVPYIRDVLV
DHGFSAKMIIGCGQYEIARLVNEYPDCAAALGKIRINPGNVGFGNKRDKNFEDIVEFAIKHDIPIRIGVNWGSLDKYLAS
KLMNDNALLINPKPDYIVLQKALVISAITSAKRAEEIGLSKNKIVISCKTSKIQDLIPVYTVLSNVCNYPLHLGLTEAGS
GTKGMVSSAAGISYLLLNGIGDTIRVSLTQQPGEARSIEVKLCQEILQSIGLRNFSAQVTSCPGCNRTNPKYFHQLAKDI
NDYIKQRMPVWRNDNPGSENMTVAVMGCIVNGPGESKHANLGISLPGYGERPVAAVYQNGEKLCTLEGGNIFEQFVSIIE
NYVNVYYKQ
>Mature_409_residues
MFNCIANKNKITYEVKVGDVVIGGNNPVVVQSMALGGSGDVYKDAHEVLELAQAGSELVRVAVNSEQAMKNVPYIRDVLV
DHGFSAKMIIGCGQYEIARLVNEYPDCAAALGKIRINPGNVGFGNKRDKNFEDIVEFAIKHDIPIRIGVNWGSLDKYLAS
KLMNDNALLINPKPDYIVLQKALVISAITSAKRAEEIGLSKNKIVISCKTSKIQDLIPVYTVLSNVCNYPLHLGLTEAGS
GTKGMVSSAAGISYLLLNGIGDTIRVSLTQQPGEARSIEVKLCQEILQSIGLRNFSAQVTSCPGCNRTNPKYFHQLAKDI
NDYIKQRMPVWRNDNPGSENMTVAVMGCIVNGPGESKHANLGISLPGYGERPVAAVYQNGEKLCTLEGGNIFEQFVSIIE
NYVNVYYKQ

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=408, Percent_Identity=31.1274509803922, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44547; Mature: 44547

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNCIANKNKITYEVKVGDVVIGGNNPVVVQSMALGGSGDVYKDAHEVLELAQAGSELVR
CCCCCCCCCEEEEEEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHEEE
VAVNSEQAMKNVPYIRDVLVDHGFSAKMIIGCGQYEIARLVNEYPDCAAALGKIRINPGN
EEECCHHHHHCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCHHHHHCEEEECCCC
VGFGNKRDKNFEDIVEFAIKHDIPIRIGVNWGSLDKYLASKLMNDNALLINPKPDYIVLQ
CCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCCCEEEHH
KALVISAITSAKRAEEIGLSKNKIVISCKTSKIQDLIPVYTVLSNVCNYPLHLGLTEAGS
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCC
GTKGMVSSAAGISYLLLNGIGDTIRVSLTQQPGEARSIEVKLCQEILQSIGLRNFSAQVT
CCCCHHHHHCCHHEEEECCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCCCEE
SCPGCNRTNPKYFHQLAKDINDYIKQRMPVWRNDNPGSENMTVAVMGCIVNGPGESKHAN
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCE
LGISLPGYGERPVAAVYQNGEKLCTLEGGNIFEQFVSIIENYVNVYYKQ
EEEECCCCCCCCHHHEECCCCEEEEEECCHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MFNCIANKNKITYEVKVGDVVIGGNNPVVVQSMALGGSGDVYKDAHEVLELAQAGSELVR
CCCCCCCCCEEEEEEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCHHEEE
VAVNSEQAMKNVPYIRDVLVDHGFSAKMIIGCGQYEIARLVNEYPDCAAALGKIRINPGN
EEECCHHHHHCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCHHHHHCEEEECCCC
VGFGNKRDKNFEDIVEFAIKHDIPIRIGVNWGSLDKYLASKLMNDNALLINPKPDYIVLQ
CCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEEEECCCCCEEEHH
KALVISAITSAKRAEEIGLSKNKIVISCKTSKIQDLIPVYTVLSNVCNYPLHLGLTEAGS
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCC
GTKGMVSSAAGISYLLLNGIGDTIRVSLTQQPGEARSIEVKLCQEILQSIGLRNFSAQVT
CCCCHHHHHCCHHEEEECCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCCCEE
SCPGCNRTNPKYFHQLAKDINDYIKQRMPVWRNDNPGSENMTVAVMGCIVNGPGESKHAN
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCE
LGISLPGYGERPVAAVYQNGEKLCTLEGGNIFEQFVSIIENYVNVYYKQ
EEEECCCCCCCCHHHEECCCCEEEEEECCHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA