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The map label for this gene is prfA [H]

Identifier: 88657945

GI number: 88657945

Start: 602148

End: 603227

Strand: Direct

Name: prfA [H]

Synonym: ECH_0597

Alternate gene names: 88657945

Gene position: 602148-603227 (Clockwise)

Preceding gene: 88658410

Following gene: 88657666

Centisome position: 51.19

GC content: 32.22

Gene sequence:

>1080_bases
ATGAGCTTTGATAATAGTTTAGAAGAGTTGTCTCAAAAATTCTACAAACTAAAAAGTATGTTAGAAGATCCAAGTCAATT
GAGTGTGGATTCCTTTGTTGCTGCTTCAAAAGAATATTCAGAATTATTGCCTGTGATATCAGTGATAGACCAATATAATA
TCTTACAAAAAGATATAGCAGGCTTAGAAGAACTGATAAATAATCCAGAAACTGATCATGAATTAAAAAGTCTAGCTAAA
GAAGAATTCTATGAACGGCAAAAACAATTACCTAAAGTTAAGCATAAATTAAAATTATCCTTACTTCCCAAGGATAAAGA
TGATGCACGTAATGCTATTTTAGAAATTAGAGCAGGTACAGGTGGAGAAGAAGCTGCATTATTTGTGACTGATTTATATA
GAATGTATACAAAATATGCTGAACAAAAGAATTGGAAATTTGAACAGATTAACTCATCTTCAACCGGTATAGGCGGACAT
AAGGAAATATCATTATGTATAAGCGGATCTAATGTATTTGCAAGGTTAAAATTTGAATCTGGAGTGCATAGAGTACAAAG
GGTACCGGAAACTGAAGCTTCTGGAAGACTTCATACTTCAGCTGCTACAGTAGCAGTTTTACCAGAAATTGAAGAAGTAG
ATTTAAAGATAGATGAAAAAGATTTAAGAATAGATGTATATCGTTCAAGCGGTCCAGGAGGACAATCTGTGAATACTACT
GATAGTGCTGTACGTATTACGCATATACCAAGCGGAATTGTCGTTATACAGCAAGATGAGAAATCTCAACATAAAAATAA
AAGTAAAGCTCTTAAGGTATTAAGAGCAAGGCTTTATAACCTAGAAAAACAAAAAAGAGATGCAGAAATTTCACAAATGA
GAAAAAGTCAGATAGGATCAGGAGACCGTTCTGAGCGTATAAGAACTTACAATTTTCCTCAATCTAGAATTACAGATCAT
AGGATAAATCTTACATTATATAGATTAGATGATATTATGAAAGAAGGAAATTTGGATGAGTTTATTGAAGCATTAATAGC
CGAAGATGAAGCAAATAAATTAAAGAACCTGCATATTTGA

Upstream 100 bases:

>100_bases
CATATAAAAATTTAAAGAAAAAAATTTTGCAATTAATATTACAAATGATATTATCTAGCAAAAAGTAGATAAAGATTATT
AATTGAGTGGTGACAAAGCT

Downstream 100 bases:

>100_bases
TCAAAAAAACTTTTACAAACCAGATTAGATTACTTTTTAAAGAATTACTGTAGGGTTCATCAATGGCTTAATATTAGCAA
TTGTGTCTTTTGTAGCACTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSFDNSLEELSQKFYKLKSMLEDPSQLSVDSFVAASKEYSELLPVISVIDQYNILQKDIAGLEELINNPETDHELKSLAK
EEFYERQKQLPKVKHKLKLSLLPKDKDDARNAILEIRAGTGGEEAALFVTDLYRMYTKYAEQKNWKFEQINSSSTGIGGH
KEISLCISGSNVFARLKFESGVHRVQRVPETEASGRLHTSAATVAVLPEIEEVDLKIDEKDLRIDVYRSSGPGGQSVNTT
DSAVRITHIPSGIVVIQQDEKSQHKNKSKALKVLRARLYNLEKQKRDAEISQMRKSQIGSGDRSERIRTYNFPQSRITDH
RINLTLYRLDDIMKEGNLDEFIEALIAEDEANKLKNLHI

Sequences:

>Translated_359_residues
MSFDNSLEELSQKFYKLKSMLEDPSQLSVDSFVAASKEYSELLPVISVIDQYNILQKDIAGLEELINNPETDHELKSLAK
EEFYERQKQLPKVKHKLKLSLLPKDKDDARNAILEIRAGTGGEEAALFVTDLYRMYTKYAEQKNWKFEQINSSSTGIGGH
KEISLCISGSNVFARLKFESGVHRVQRVPETEASGRLHTSAATVAVLPEIEEVDLKIDEKDLRIDVYRSSGPGGQSVNTT
DSAVRITHIPSGIVVIQQDEKSQHKNKSKALKVLRARLYNLEKQKRDAEISQMRKSQIGSGDRSERIRTYNFPQSRITDH
RINLTLYRLDDIMKEGNLDEFIEALIAEDEANKLKNLHI
>Mature_358_residues
SFDNSLEELSQKFYKLKSMLEDPSQLSVDSFVAASKEYSELLPVISVIDQYNILQKDIAGLEELINNPETDHELKSLAKE
EFYERQKQLPKVKHKLKLSLLPKDKDDARNAILEIRAGTGGEEAALFVTDLYRMYTKYAEQKNWKFEQINSSSTGIGGHK
EISLCISGSNVFARLKFESGVHRVQRVPETEASGRLHTSAATVAVLPEIEEVDLKIDEKDLRIDVYRSSGPGGQSVNTTD
SAVRITHIPSGIVVIQQDEKSQHKNKSKALKVLRARLYNLEKQKRDAEISQMRKSQIGSGDRSERIRTYNFPQSRITDHR
INLTLYRLDDIMKEGNLDEFIEALIAEDEANKLKNLHI

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=341, Percent_Identity=44.574780058651, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI34577120, Length=357, Percent_Identity=39.7759103641457, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI166795305, Length=245, Percent_Identity=42.4489795918367, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI1787462, Length=353, Percent_Identity=51.5580736543909, Blast_Score=354, Evalue=6e-99,
Organism=Escherichia coli, GI2367172, Length=317, Percent_Identity=41.0094637223975, Blast_Score=212, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=290, Percent_Identity=37.2413793103448, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=337, Percent_Identity=43.026706231454, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI19921226, Length=359, Percent_Identity=42.0612813370474, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40807; Mature: 40675

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDNSLEELSQKFYKLKSMLEDPSQLSVDSFVAASKEYSELLPVISVIDQYNILQKDIA
CCCCCHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLEELINNPETDHELKSLAKEEFYERQKQLPKVKHKLKLSLLPKDKDDARNAILEIRAGT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHEEEEEECCC
GGEEAALFVTDLYRMYTKYAEQKNWKFEQINSSSTGIGGHKEISLCISGSNVFARLKFES
CCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEEECCCEEEEEEHHH
GVHRVQRVPETEASGRLHTSAATVAVLPEIEEVDLKIDEKDLRIDVYRSSGPGGQSVNTT
HHHHHHHCCCCCCCCCEECCCEEEEECCCHHHCCCEECCCCCEEEEEECCCCCCCCCCCC
DSAVRITHIPSGIVVIQQDEKSQHKNKSKALKVLRARLYNLEKQKRDAEISQMRKSQIGS
CCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNFPQSRITDHRINLTLYRLDDIMKEGNLDEFIEALIAEDEANKLKNLHI
CCCCCCCEECCCCHHHCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure 
SFDNSLEELSQKFYKLKSMLEDPSQLSVDSFVAASKEYSELLPVISVIDQYNILQKDIA
CCCCHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLEELINNPETDHELKSLAKEEFYERQKQLPKVKHKLKLSLLPKDKDDARNAILEIRAGT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHEEEEEECCC
GGEEAALFVTDLYRMYTKYAEQKNWKFEQINSSSTGIGGHKEISLCISGSNVFARLKFES
CCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEEECCCEEEEEEHHH
GVHRVQRVPETEASGRLHTSAATVAVLPEIEEVDLKIDEKDLRIDVYRSSGPGGQSVNTT
HHHHHHHCCCCCCCCCEECCCEEEEECCCHHHCCCEECCCCCEEEEEECCCCCCCCCCCC
DSAVRITHIPSGIVVIQQDEKSQHKNKSKALKVLRARLYNLEKQKRDAEISQMRKSQIGS
CCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNFPQSRITDHRINLTLYRLDDIMKEGNLDEFIEALIAEDEANKLKNLHI
CCCCCCCEECCCCHHHCCCCEEEEEEEHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA