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The map label for this gene is proP [H]

Identifier: 88657883

GI number: 88657883

Start: 184447

End: 185715

Strand: Direct

Name: proP [H]

Synonym: ECH_0193

Alternate gene names: 88657883

Gene position: 184447-185715 (Clockwise)

Preceding gene: 88657735

Following gene: 88657667

Centisome position: 15.68

GC content: 31.84

Gene sequence:

>1269_bases
ATGAATTTAAGAAGAGCTGTGTTATCGACAATCATGTGTAATACTTTAGTATGGTATGATTATGTGTTATTTGGGAATTT
GGTGAGTGTAATCAGTAAATTATTTTTTCCAGCAGAAGATAGATATTTTAGTCTTATTATGACATTCAGTATTTTTGCAG
TTGGATTTTTAATGCGTCCTTTTGGGGCAAGTATTTTTGGTTACATTGGCGATAAATATGGAAGAAAAGCTGCACTGACT
TTATCGATTATAGCAATATCTGTCCCTATTACTTTTATCTCAATATTGCCTACCTATGAAAAAATAGGAATATTGTCTCC
TATATTACTTATTATTTGTAGGTTGATGCAGGGGATATCTCTAGGTGGAGAAGCTGGTAATGCTACTTTCTTAATAGAGC
ATTCTAAAAAGGGAAAAAACATTGGTTTTTTTGGTAGTTTTGAGACCCTTAGTGCTGTGCTTGGTTCTATTATTGCATTA
TTTATGATCTTGTTATCTCAGTACTTTACAGGAGAAAATTTTGAAGTATGGGGTTGGAGAATACCTTTTGTAATTGGTTT
ATTATTGGGATTAATTAGTGTTTATATTAGGCGTATTACTGGTGAAAGTCCTGCGTATGATACTCATAAAGAAAATAATA
ATCTTTCTCAATCTCCTTTCTTAGAATTGTTAAAAAAGTATAAGCGCCCTTTAGTCTTGGCAACATGTATTGACTGTGTA
GAAAATTGTTCATTTCATATTTTTATGGTGTTTTTTATTACATTTATTAAGGAGTTCTCAAATATTCACCTGAATTTAAA
TGCTAATACTATAAGCATTATTGAAAGTTTTAATATAATGATTTGTGGTATTTTGAATGTATTTTTTGGATATATTTCAG
ATTATGTAGGGCGTAGAAAAGTAATGTTAATTGCATCTGTGTCATTGTTTTGTGTTGCAATACCAGTATTTTGGTTATTA
AGTCAAGATAGCTATGTTTCTTTGATTGCTGCATATTTAATATTTGTAATTCCGTTTTCTGCAACTTTAGGTCCAGCAAG
TGGTGCAATGTCTGAATTGTTCCCTACAAAAGTTAGATATACTGGTTTTGGATTATCGCGTAACATTGCTTCAGCTATAT
CTGGTGGTATGGCTCCTGTAGTATGTACATGGCTTATAAGGGCAACAGGGCTTTCGTTTATTCCTGGAGTATATGTTATG
TTTTGGGCATTGGTTGGAGTTATTGCATTGTGTCAGATCAGAAAAAAAGATGTTTATGCTGATTGGTAA

Upstream 100 bases:

>100_bases
AAAAGCAATATGATTAATTAGTCAAGCTTGTTCAAATTATGAAAAAGTGAAAAGATGTCCTTTTTGAAAAGGATATGTAA
TAGGAGGATATTTAAGTTAT

Downstream 100 bases:

>100_bases
TCTACTATTTAAATGTTGTGCATTGATCTAATGTAGTAGCTAAAATGATATTCTCACTAGAATGAATAGGGTTTTTGCGT
GGATATTTTGTTATATCTGG

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNLRRAVLSTIMCNTLVWYDYVLFGNLVSVISKLFFPAEDRYFSLIMTFSIFAVGFLMRPFGASIFGYIGDKYGRKAALT
LSIIAISVPITFISILPTYEKIGILSPILLIICRLMQGISLGGEAGNATFLIEHSKKGKNIGFFGSFETLSAVLGSIIAL
FMILLSQYFTGENFEVWGWRIPFVIGLLLGLISVYIRRITGESPAYDTHKENNNLSQSPFLELLKKYKRPLVLATCIDCV
ENCSFHIFMVFFITFIKEFSNIHLNLNANTISIIESFNIMICGILNVFFGYISDYVGRRKVMLIASVSLFCVAIPVFWLL
SQDSYVSLIAAYLIFVIPFSATLGPASGAMSELFPTKVRYTGFGLSRNIASAISGGMAPVVCTWLIRATGLSFIPGVYVM
FWALVGVIALCQIRKKDVYADW

Sequences:

>Translated_422_residues
MNLRRAVLSTIMCNTLVWYDYVLFGNLVSVISKLFFPAEDRYFSLIMTFSIFAVGFLMRPFGASIFGYIGDKYGRKAALT
LSIIAISVPITFISILPTYEKIGILSPILLIICRLMQGISLGGEAGNATFLIEHSKKGKNIGFFGSFETLSAVLGSIIAL
FMILLSQYFTGENFEVWGWRIPFVIGLLLGLISVYIRRITGESPAYDTHKENNNLSQSPFLELLKKYKRPLVLATCIDCV
ENCSFHIFMVFFITFIKEFSNIHLNLNANTISIIESFNIMICGILNVFFGYISDYVGRRKVMLIASVSLFCVAIPVFWLL
SQDSYVSLIAAYLIFVIPFSATLGPASGAMSELFPTKVRYTGFGLSRNIASAISGGMAPVVCTWLIRATGLSFIPGVYVM
FWALVGVIALCQIRKKDVYADW
>Mature_422_residues
MNLRRAVLSTIMCNTLVWYDYVLFGNLVSVISKLFFPAEDRYFSLIMTFSIFAVGFLMRPFGASIFGYIGDKYGRKAALT
LSIIAISVPITFISILPTYEKIGILSPILLIICRLMQGISLGGEAGNATFLIEHSKKGKNIGFFGSFETLSAVLGSIIAL
FMILLSQYFTGENFEVWGWRIPFVIGLLLGLISVYIRRITGESPAYDTHKENNNLSQSPFLELLKKYKRPLVLATCIDCV
ENCSFHIFMVFFITFIKEFSNIHLNLNANTISIIESFNIMICGILNVFFGYISDYVGRRKVMLIASVSLFCVAIPVFWLL
SQDSYVSLIAAYLIFVIPFSATLGPASGAMSELFPTKVRYTGFGLSRNIASAISGGMAPVVCTWLIRATGLSFIPGVYVM
FWALVGVIALCQIRKKDVYADW

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=33.1002331002331, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789941, Length=420, Percent_Identity=31.9047619047619, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1788292, Length=398, Percent_Identity=27.1356783919598, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1788942, Length=393, Percent_Identity=30.5343511450382, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI87082404, Length=400, Percent_Identity=27.75, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082231, Length=423, Percent_Identity=21.5130023640662, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47011; Mature: 47011

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRRAVLSTIMCNTLVWYDYVLFGNLVSVISKLFFPAEDRYFSLIMTFSIFAVGFLMRP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FGASIFGYIGDKYGRKAALTLSIIAISVPITFISILPTYEKIGILSPILLIICRLMQGIS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
LGGEAGNATFLIEHSKKGKNIGFFGSFETLSAVLGSIIALFMILLSQYFTGENFEVWGWR
CCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCH
IPFVIGLLLGLISVYIRRITGESPAYDTHKENNNLSQSPFLELLKKYKRPLVLATCIDCV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
ENCSFHIFMVFFITFIKEFSNIHLNLNANTISIIESFNIMICGILNVFFGYISDYVGRRK
HCCCHHHHHHHHHHHHHHHCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VMLIASVSLFCVAIPVFWLLSQDSYVSLIAAYLIFVIPFSATLGPASGAMSELFPTKVRY
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEE
TGFGLSRNIASAISGGMAPVVCTWLIRATGLSFIPGVYVMFWALVGVIALCQIRKKDVYA
ECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DW
CC
>Mature Secondary Structure
MNLRRAVLSTIMCNTLVWYDYVLFGNLVSVISKLFFPAEDRYFSLIMTFSIFAVGFLMRP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FGASIFGYIGDKYGRKAALTLSIIAISVPITFISILPTYEKIGILSPILLIICRLMQGIS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
LGGEAGNATFLIEHSKKGKNIGFFGSFETLSAVLGSIIALFMILLSQYFTGENFEVWGWR
CCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCH
IPFVIGLLLGLISVYIRRITGESPAYDTHKENNNLSQSPFLELLKKYKRPLVLATCIDCV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
ENCSFHIFMVFFITFIKEFSNIHLNLNANTISIIESFNIMICGILNVFFGYISDYVGRRK
HCCCHHHHHHHHHHHHHHHCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCH
VMLIASVSLFCVAIPVFWLLSQDSYVSLIAAYLIFVIPFSATLGPASGAMSELFPTKVRY
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEE
TGFGLSRNIASAISGGMAPVVCTWLIRATGLSFIPGVYVMFWALVGVIALCQIRKKDVYA
ECCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DW
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]