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The map label for this gene is xth [H]

Identifier: 88657831

GI number: 88657831

Start: 685485

End: 686330

Strand: Reverse

Name: xth [H]

Synonym: ECH_0675

Alternate gene names: 88657831

Gene position: 686330-685485 (Counterclockwise)

Preceding gene: 88658175

Following gene: 88657890

Centisome position: 58.35

GC content: 30.97

Gene sequence:

>846_bases
ATGACTTTAAAAATAGCTACATGGAATGTCAACTCTATTAGGAAAAGGTTGGATCATTTATGTAATTGGCTAATTAATAG
TGCTATTGATATAGCGTTGTTGCAAGAAATAAAGTGTACAGATGAACAGTTTCCTTTTTTTGATGTGGAATCATTAGGAT
ATAAGTGCTATGTACATGGGCAGAAGGCTAGGAATGGAGTAGCCATTATAACAAGGTATCCTATAGTTGGGGAATTGGTT
ACAAGTATTTTTTCATCTGATTACAAATCTAAGTTATGTTACGAATTAAGTTCAGAGAATTTTATTTATAATTGTGATGA
GTCTCGTTATTTGGAGTGTGTAGTTCTACATCATAATATTAAAATTCGTATTGCTAGTATATATGTTCCTAATGGACAAA
GTATTGATTCTGATGCTTTTCAATATAAACTTGGGTTTTTTGATCAATTAAGAGAACATGCATTATCTTTACTTAAAAAA
GAAGAAATTTTAATATTAGGTGGCGATTATAATGTAGCACCTTATCCTATAGATGTCTATGATCCTGAAGTTATGGATGG
TAAATTATGTTTTCATAAGTCTGAAAGAGAAAAGTTTCGATCTATTTTGAATTTAGGGTTTACAGATTCTTTTCGTGTAT
TGAATGACTATGAAAAGAAATTTAGTTGGTGGAATTATAAAGCAGGTGCATGGCAGCAAAATAGGGGTTTAAGAATAGAT
AACTTGTTATTATCACCACAAGCTACAGATAAGCTATTGTCTTGTGTTATTCATGATAAACTTAGAGGATTGGATACACC
TTCTGACCATGCTCCAGTTGTATGTGAACTTGATTTAAGTTCTTAA

Upstream 100 bases:

>100_bases
TTTCGATGCAACTGTGTGGACCTGTGATTTAACTCATAGATTTGTTGATATTAATGGTAAGTATTTAACTTAATTTGATA
AGTAGTTTTTAGAGATCACA

Downstream 100 bases:

>100_bases
TGATTAATTAAAGATTATAAATATTTTAGCATTATAATATAAGTATACTGCTTATCAATATTGAGCATTTATATTTTACA
ACATTAATATATATTTTGTT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTLKIATWNVNSIRKRLDHLCNWLINSAIDIALLQEIKCTDEQFPFFDVESLGYKCYVHGQKARNGVAIITRYPIVGELV
TSIFSSDYKSKLCYELSSENFIYNCDESRYLECVVLHHNIKIRIASIYVPNGQSIDSDAFQYKLGFFDQLREHALSLLKK
EEILILGGDYNVAPYPIDVYDPEVMDGKLCFHKSEREKFRSILNLGFTDSFRVLNDYEKKFSWWNYKAGAWQQNRGLRID
NLLLSPQATDKLLSCVIHDKLRGLDTPSDHAPVVCELDLSS

Sequences:

>Translated_281_residues
MTLKIATWNVNSIRKRLDHLCNWLINSAIDIALLQEIKCTDEQFPFFDVESLGYKCYVHGQKARNGVAIITRYPIVGELV
TSIFSSDYKSKLCYELSSENFIYNCDESRYLECVVLHHNIKIRIASIYVPNGQSIDSDAFQYKLGFFDQLREHALSLLKK
EEILILGGDYNVAPYPIDVYDPEVMDGKLCFHKSEREKFRSILNLGFTDSFRVLNDYEKKFSWWNYKAGAWQQNRGLRID
NLLLSPQATDKLLSCVIHDKLRGLDTPSDHAPVVCELDLSS
>Mature_280_residues
TLKIATWNVNSIRKRLDHLCNWLINSAIDIALLQEIKCTDEQFPFFDVESLGYKCYVHGQKARNGVAIITRYPIVGELVT
SIFSSDYKSKLCYELSSENFIYNCDESRYLECVVLHHNIKIRIASIYVPNGQSIDSDAFQYKLGFFDQLREHALSLLKKE
EILILGGDYNVAPYPIDVYDPEVMDGKLCFHKSEREKFRSILNLGFTDSFRVLNDYEKKFSWWNYKAGAWQQNRGLRIDN
LLLSPQATDKLLSCVIHDKLRGLDTPSDHAPVVCELDLSS

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375507, Length=326, Percent_Identity=25.4601226993865, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788046, Length=280, Percent_Identity=29.6428571428571, Blast_Score=121, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71989536, Length=138, Percent_Identity=36.231884057971, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI221330655, Length=279, Percent_Identity=29.7491039426523, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17136678, Length=279, Percent_Identity=29.7491039426523, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32409; Mature: 32278

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKIATWNVNSIRKRLDHLCNWLINSAIDIALLQEIKCTDEQFPFFDVESLGYKCYVHG
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCEEEEEEC
QKARNGVAIITRYPIVGELVTSIFSSDYKSKLCYELSSENFIYNCDESRYLECVVLHHNI
CCCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHEEECCCCEEEECCCCCEEEEEEEECCE
KIRIASIYVPNGQSIDSDAFQYKLGFFDQLREHALSLLKKEEILILGGDYNVAPYPIDVY
EEEEEEEEECCCCCCCCCHHEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECC
DPEVMDGKLCFHKSEREKFRSILNLGFTDSFRVLNDYEKKFSWWNYKAGAWQQNRGLRID
CCCCCCCEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCCEEH
NLLLSPQATDKLLSCVIHDKLRGLDTPSDHAPVVCELDLSS
HEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
TLKIATWNVNSIRKRLDHLCNWLINSAIDIALLQEIKCTDEQFPFFDVESLGYKCYVHG
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCEEEEEEC
QKARNGVAIITRYPIVGELVTSIFSSDYKSKLCYELSSENFIYNCDESRYLECVVLHHNI
CCCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHEEECCCCEEEECCCCCEEEEEEEECCE
KIRIASIYVPNGQSIDSDAFQYKLGFFDQLREHALSLLKKEEILILGGDYNVAPYPIDVY
EEEEEEEEECCCCCCCCCHHEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECC
DPEVMDGKLCFHKSEREKFRSILNLGFTDSFRVLNDYEKKFSWWNYKAGAWQQNRGLRID
CCCCCCCEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCCEEH
NLLLSPQATDKLLSCVIHDKLRGLDTPSDHAPVVCELDLSS
HEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]