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The map label for this gene is lipA

Identifier: 88657773

GI number: 88657773

Start: 477049

End: 477942

Strand: Direct

Name: lipA

Synonym: ECH_0490

Alternate gene names: 88657773

Gene position: 477049-477942 (Clockwise)

Preceding gene: 88657885

Following gene: 88658263

Centisome position: 40.56

GC content: 34.34

Gene sequence:

>894_bases
ATGAGAAGTAAACCTGATTGGTTAAAAGTTAAGATGCCTACAGGGGATACTTTTTATCAAGTTCGCAATTTAATGAAATT
GTATAAATTAAATACTGTGTGTGAAGAAGCAGCTTGTCCCAACATTGGTGAGTGTTGGAATAAAAGACATGCAACAGTAA
TGATTTTAGGTTCTACATGTACTAGAGCATGTGCATTTTGTAATGTTGTTTCTGGAATTCCAGATAAACTGGATCCTCAT
GAGCCTCAAAACTTAGCTAAGGCTGTTGGTTTACTTAAACTTGAGCACGTTGTTATTACATCTGTAGACAGGGATGACTT
GGAAGATGGTGGATCTGGACATTTTGTAGAGTGTATAGAAGAAATACGAAAAAACGATCAGAATGTTACTATTGAAGTAT
TAACTCCAGATTTTTTGAATAAACATGGTGCAATTGAAAAGGTTGCTGATGCTGCTCCTGATGTTTACAATCATAATATA
GAAACTGTTCCAAGGTTGTATGCAAAAATTAGACCAAAAGCACGTTACTTTCATTCTCTTTATTTGTTAAAAACAGTTAA
ATATAAAAATCCTAAAGTATTTACTAAATCTGGAATTATGGTAGGATTGGGTGAAACAAAAGAAGAAATATATCAAGTAA
TGAATGATTTAAGATCTGCGGATGTTGATTTTATAACGATTGGTCAATATTTGCAGCCTACTCCTAAGCATGCTGCAGTT
GATAGGTATGTAACTCCTGAAGAATTTGATCATTACAAGTATGTTGCATACTCTAAAGGGTTTTTAATGGTTGCGTCGGG
CCCTTTAGTAAGGTCTTCATACCATGCTGGAGAGGATTTTCAAAGGTTAAAGAAAAATCGTGCTGCTATGTTCATGCATG
CTAAAAGCAATTAG

Upstream 100 bases:

>100_bases
ACACTTAATAAACTGTAAGTGGTATCAAATGTTATCCTTTCTTGACATTAATACCACTGTTATTTATACTCAACTAATAA
GAATTTCTAAATTTTACTGT

Downstream 100 bases:

>100_bases
AATTAACATGGTAGTTAATATTTTTTAGAAGTTTGAATGTTTATACCAGAAGGGTTTTTCATTAGTTTTATATGATATTT
AATTTTTTAGATAGTTAATC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRSKPDWLKVKMPTGDTFYQVRNLMKLYKLNTVCEEAACPNIGECWNKRHATVMILGSTCTRACAFCNVVSGIPDKLDPH
EPQNLAKAVGLLKLEHVVITSVDRDDLEDGGSGHFVECIEEIRKNDQNVTIEVLTPDFLNKHGAIEKVADAAPDVYNHNI
ETVPRLYAKIRPKARYFHSLYLLKTVKYKNPKVFTKSGIMVGLGETKEEIYQVMNDLRSADVDFITIGQYLQPTPKHAAV
DRYVTPEEFDHYKYVAYSKGFLMVASGPLVRSSYHAGEDFQRLKKNRAAMFMHAKSN

Sequences:

>Translated_297_residues
MRSKPDWLKVKMPTGDTFYQVRNLMKLYKLNTVCEEAACPNIGECWNKRHATVMILGSTCTRACAFCNVVSGIPDKLDPH
EPQNLAKAVGLLKLEHVVITSVDRDDLEDGGSGHFVECIEEIRKNDQNVTIEVLTPDFLNKHGAIEKVADAAPDVYNHNI
ETVPRLYAKIRPKARYFHSLYLLKTVKYKNPKVFTKSGIMVGLGETKEEIYQVMNDLRSADVDFITIGQYLQPTPKHAAV
DRYVTPEEFDHYKYVAYSKGFLMVASGPLVRSSYHAGEDFQRLKKNRAAMFMHAKSN
>Mature_297_residues
MRSKPDWLKVKMPTGDTFYQVRNLMKLYKLNTVCEEAACPNIGECWNKRHATVMILGSTCTRACAFCNVVSGIPDKLDPH
EPQNLAKAVGLLKLEHVVITSVDRDDLEDGGSGHFVECIEEIRKNDQNVTIEVLTPDFLNKHGAIEKVADAAPDVYNHNI
ETVPRLYAKIRPKARYFHSLYLLKTVKYKNPKVFTKSGIMVGLGETKEEIYQVMNDLRSADVDFITIGQYLQPTPKHAAV
DRYVTPEEFDHYKYVAYSKGFLMVASGPLVRSSYHAGEDFQRLKKNRAAMFMHAKSN

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=283, Percent_Identity=48.4098939929329, Blast_Score=281, Evalue=5e-76,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=47.1311475409836, Blast_Score=237, Evalue=7e-63,
Organism=Escherichia coli, GI1786846, Length=275, Percent_Identity=50.9090909090909, Blast_Score=290, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI32564533, Length=254, Percent_Identity=49.2125984251969, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324770, Length=283, Percent_Identity=47.3498233215548, Blast_Score=271, Evalue=1e-73,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=49.1166077738516, Blast_Score=281, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_EHRCR (Q2GGX7)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507306.1
- ProteinModelPortal:   Q2GGX7
- STRING:   Q2GGX7
- GeneID:   3927088
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0490
- TIGR:   ECH_0490
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q2GGX7
- ProtClustDB:   PRK05481
- BioCyc:   ECHA205920:ECH_0490-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 33676; Mature: 33676

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKPDWLKVKMPTGDTFYQVRNLMKLYKLNTVCEEAACPNIGECWNKRHATVMILGSTC
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCHH
TRACAFCNVVSGIPDKLDPHEPQNLAKAVGLLKLEHVVITSVDRDDLEDGGSGHFVECIE
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHCCCCCCHHHHHHH
EIRKNDQNVTIEVLTPDFLNKHGAIEKVADAAPDVYNHNIETVPRLYAKIRPKARYFHSL
HHHCCCCCEEEEEECHHHHHHCCCHHHHHHCCCHHHCCCHHHHHHHHHHHCHHHHHHHHH
YLLKTVKYKNPKVFTKSGIMVGLGETKEEIYQVMNDLRSADVDFITIGQYLQPTPKHAAV
HHHHHHCCCCCCEEEECCEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHCCCCCHHHH
DRYVTPEEFDHYKYVAYSKGFLMVASGPLVRSSYHAGEDFQRLKKNRAAMFMHAKSN
CCCCCHHHCCCEEEEEEECCEEEEECCCCEECCCCCCHHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure
MRSKPDWLKVKMPTGDTFYQVRNLMKLYKLNTVCEEAACPNIGECWNKRHATVMILGSTC
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCHH
TRACAFCNVVSGIPDKLDPHEPQNLAKAVGLLKLEHVVITSVDRDDLEDGGSGHFVECIE
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCHHHHCCCCCCHHHHHHH
EIRKNDQNVTIEVLTPDFLNKHGAIEKVADAAPDVYNHNIETVPRLYAKIRPKARYFHSL
HHHCCCCCEEEEEECHHHHHHCCCHHHHHHCCCHHHCCCHHHHHHHHHHHCHHHHHHHHH
YLLKTVKYKNPKVFTKSGIMVGLGETKEEIYQVMNDLRSADVDFITIGQYLQPTPKHAAV
HHHHHHCCCCCCEEEECCEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHCCCCCHHHH
DRYVTPEEFDHYKYVAYSKGFLMVASGPLVRSSYHAGEDFQRLKKNRAAMFMHAKSN
CCCCCHHHCCCEEEEEEECCEEEEECCCCEECCCCCCHHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA