Click here to switch to the map view.

The map label for this gene is pdhC [H]

Identifier: 88657701

GI number: 88657701

Start: 87593

End: 88843

Strand: Direct

Name: pdhC [H]

Synonym: ECH_0098

Alternate gene names: 88657701

Gene position: 87593-88843 (Clockwise)

Preceding gene: 88658165

Following gene: 88658652

Centisome position: 7.45

GC content: 32.61

Gene sequence:

>1251_bases
ATGCCAATAGAAGTATTGATGCCTGCTCTTTCTCCAACTATGAAAAGTGGCACTATAAGAAAGTGGTATAAAGCAGAAGG
GGATGTAGTAAAATCAGGTGATGTAATAGCTGATATAGAAACTGATAAAGCTGTTATGGAATGTGAGTATACAGATGAAG
ATGGTATCATGGGTAAAATTTTTTTTGCTGAAGGAAGCAAAAATATAGAGGTAAATCAATTGATAGCATTGATTGCTGTA
GATGAACAGGATTTAGCTAAAGTTCATTCATATGAAAAAGGTGATAATGTTGTAAAAAATGAGTTAGTTGCGTTACAAGA
TAGTCAACCTGCTCAAGATGAGTCAGTTGTATTGCAAATGAATCAACAAATTGTGAATGCTAGCGAAGTATTGGTTAATT
CATCTAATTCTTCTGAAAGAGTTAAGGTGAGTCCTTTAGCAAAAAAAATTGCTTCGAACCTTGGTGTTGATGTAAATTTG
GTAAAAGGAACAGGCCCGTACGGTAGGATTATAAAAGCTGATATATTGGATGTTATAAATCAGCATGGTCACATTGCTAA
CTCTCCTGAGGATGCTTCTTTTACTGAAATTAGTAGTATGCGTAGAGTAATAGCAGAACGTTTAGTGTATTCAAAACAAA
CAATTCCTCACTTTTACGTTTCAATAGATTGTCTTGTGGATAGTTTGTTAAAATTAAGGTTAGAAATAAATGCTGAAAAT
CCTGATACTAAGGTTACAGTGAATGATTTCATCATTAAAGCTGTTGCCATGAGTATTAAGAAATTTCCTGAAATTAATGT
ATCTTGGTCTGATGATAAGATAGTTGTATTTCCTAGTATTGATATTTCTGTTGCTGTATCTATTGATAATGGACTTATCA
CACCAATTATTTTTGGTGCAGATAAGAAATCTTTGTTAGAAATATCAAGAGAAGTGAAAGCATTAGCTAGTAAGGCTAAA
TCTGGAAAATTGAAACCTGAAGAATTTCAAGGCGGAGGTTTTACTGTTTCTAATCTTGGTATGTTTGGTATTAAAGAATT
CTACGCAATTGTTAATCCTCCACAGTCTTGTATAATGTCTGTTGGATGCTCTGAAAAACGAGCAATGGTTGTTAATGAGC
AAATATGTATTTCAAATGTTGTGACAGTAACATTATCTGTAGACCATAGAGTTATTGATGGTGTACTAGCGGCAAAATTT
TTAAATTGCTTTAAATCTTATCTAGAGAAACCATTTTTAATGTTGATATAA

Upstream 100 bases:

>100_bases
TGTTTTTCTGTTCTTTAGAAGAGAGCAATGTCTATAATTGAATTGACAATGAATGTTTAATACTGTAATGGTTTATAAAA
TGATTTTTTGATATTAAGTA

Downstream 100 bases:

>100_bases
ACAGTTTATAAGTTTTTCTTAAATTCATGCCCCTTTTTTGATTGGCAAAGTCTATTTCTACAATAATATTTATCTTACCT
TATTTTTTACTTTGTTGTGC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MPIEVLMPALSPTMKSGTIRKWYKAEGDVVKSGDVIADIETDKAVMECEYTDEDGIMGKIFFAEGSKNIEVNQLIALIAV
DEQDLAKVHSYEKGDNVVKNELVALQDSQPAQDESVVLQMNQQIVNASEVLVNSSNSSERVKVSPLAKKIASNLGVDVNL
VKGTGPYGRIIKADILDVINQHGHIANSPEDASFTEISSMRRVIAERLVYSKQTIPHFYVSIDCLVDSLLKLRLEINAEN
PDTKVTVNDFIIKAVAMSIKKFPEINVSWSDDKIVVFPSIDISVAVSIDNGLITPIIFGADKKSLLEISREVKALASKAK
SGKLKPEEFQGGGFTVSNLGMFGIKEFYAIVNPPQSCIMSVGCSEKRAMVVNEQICISNVVTVTLSVDHRVIDGVLAAKF
LNCFKSYLEKPFLMLI

Sequences:

>Translated_416_residues
MPIEVLMPALSPTMKSGTIRKWYKAEGDVVKSGDVIADIETDKAVMECEYTDEDGIMGKIFFAEGSKNIEVNQLIALIAV
DEQDLAKVHSYEKGDNVVKNELVALQDSQPAQDESVVLQMNQQIVNASEVLVNSSNSSERVKVSPLAKKIASNLGVDVNL
VKGTGPYGRIIKADILDVINQHGHIANSPEDASFTEISSMRRVIAERLVYSKQTIPHFYVSIDCLVDSLLKLRLEINAEN
PDTKVTVNDFIIKAVAMSIKKFPEINVSWSDDKIVVFPSIDISVAVSIDNGLITPIIFGADKKSLLEISREVKALASKAK
SGKLKPEEFQGGGFTVSNLGMFGIKEFYAIVNPPQSCIMSVGCSEKRAMVVNEQICISNVVTVTLSVDHRVIDGVLAAKF
LNCFKSYLEKPFLMLI
>Mature_415_residues
PIEVLMPALSPTMKSGTIRKWYKAEGDVVKSGDVIADIETDKAVMECEYTDEDGIMGKIFFAEGSKNIEVNQLIALIAVD
EQDLAKVHSYEKGDNVVKNELVALQDSQPAQDESVVLQMNQQIVNASEVLVNSSNSSERVKVSPLAKKIASNLGVDVNLV
KGTGPYGRIIKADILDVINQHGHIANSPEDASFTEISSMRRVIAERLVYSKQTIPHFYVSIDCLVDSLLKLRLEINAENP
DTKVTVNDFIIKAVAMSIKKFPEINVSWSDDKIVVFPSIDISVAVSIDNGLITPIIFGADKKSLLEISREVKALASKAKS
GKLKPEEFQGGGFTVSNLGMFGIKEFYAIVNPPQSCIMSVGCSEKRAMVVNEQICISNVVTVTLSVDHRVIDGVLAAKFL
NCFKSYLEKPFLMLI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=433, Percent_Identity=41.5704387990762, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI203098816, Length=462, Percent_Identity=39.1774891774892, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI203098753, Length=462, Percent_Identity=39.1774891774892, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI260898739, Length=178, Percent_Identity=45.5056179775281, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=25.8064516129032, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=29.646017699115, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1786946, Length=436, Percent_Identity=27.2935779816514, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1786305, Length=296, Percent_Identity=30.4054054054054, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=42.0323325635104, Blast_Score=299, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI17538894, Length=313, Percent_Identity=34.5047923322684, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=27.8422273781903, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=26.3657957244656, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324258, Length=445, Percent_Identity=35.7303370786517, Blast_Score=259, Evalue=8e-70,
Organism=Saccharomyces cerevisiae, GI6320352, Length=413, Percent_Identity=28.0871670702179, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321632, Length=189, Percent_Identity=28.042328042328, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20129315, Length=435, Percent_Identity=40.2298850574713, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24582497, Length=424, Percent_Identity=39.3867924528302, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=26.056338028169, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24645909, Length=163, Percent_Identity=33.7423312883436, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45574; Mature: 45443

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIEVLMPALSPTMKSGTIRKWYKAEGDVVKSGDVIADIETDKAVMECEYTDEDGIMGKI
CCHHHEEHHCCCCCCCCCCHHHHCCCCCEEECCCEEEEECCCCEEEEEEECCCCCCEEEE
FFAEGSKNIEVNQLIALIAVDEQDLAKVHSYEKGDNVVKNELVALQDSQPAQDESVVLQM
EEECCCCCCCHHHEEEEEEECHHHHHHHHCCCCCCCHHHHCEEEECCCCCCCCCHHHEEH
NQQIVNASEVLVNSSNSSERVKVSPLAKKIASNLGVDVNLVKGTGPYGRIIKADILDVIN
HHHHHCHHHHEEECCCCCCEEEECHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHH
QHGHIANSPEDASFTEISSMRRVIAERLVYSKQTIPHFYVSIDCLVDSLLKLRLEINAEN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHEEEEEECCC
PDTKVTVNDFIIKAVAMSIKKFPEINVSWSDDKIVVFPSIDISVAVSIDNGLITPIIFGA
CCCEEEHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCEEEEEEECCCCEEEEEECC
DKKSLLEISREVKALASKAKSGKLKPEEFQGGGFTVSNLGMFGIKEFYAIVNPPQSCIMS
CHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHH
VGCSEKRAMVVNEQICISNVVTVTLSVDHRVIDGVLAAKFLNCFKSYLEKPFLMLI
CCCCCCCEEEECCHHEECEEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
PIEVLMPALSPTMKSGTIRKWYKAEGDVVKSGDVIADIETDKAVMECEYTDEDGIMGKI
CHHHEEHHCCCCCCCCCCHHHHCCCCCEEECCCEEEEECCCCEEEEEEECCCCCCEEEE
FFAEGSKNIEVNQLIALIAVDEQDLAKVHSYEKGDNVVKNELVALQDSQPAQDESVVLQM
EEECCCCCCCHHHEEEEEEECHHHHHHHHCCCCCCCHHHHCEEEECCCCCCCCCHHHEEH
NQQIVNASEVLVNSSNSSERVKVSPLAKKIASNLGVDVNLVKGTGPYGRIIKADILDVIN
HHHHHCHHHHEEECCCCCCEEEECHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHH
QHGHIANSPEDASFTEISSMRRVIAERLVYSKQTIPHFYVSIDCLVDSLLKLRLEINAEN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHEEEEEECCC
PDTKVTVNDFIIKAVAMSIKKFPEINVSWSDDKIVVFPSIDISVAVSIDNGLITPIIFGA
CCCEEEHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCEEEEEEECCCCEEEEEECC
DKKSLLEISREVKALASKAKSGKLKPEEFQGGGFTVSNLGMFGIKEFYAIVNPPQSCIMS
CHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHH
VGCSEKRAMVVNEQICISNVVTVTLSVDHRVIDGVLAAKFLNCFKSYLEKPFLMLI
CCCCCCCEEEECCHHEECEEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]