Click here to switch to the map view.

The map label for this gene is rnhA

Identifier: 88657670

GI number: 88657670

Start: 246522

End: 246962

Strand: Direct

Name: rnhA

Synonym: ECH_0263

Alternate gene names: 88657670

Gene position: 246522-246962 (Clockwise)

Preceding gene: 88657801

Following gene: 88658277

Centisome position: 20.96

GC content: 36.51

Gene sequence:

>441_bases
ATGAAAGATGAACTGAATAAAGTAGTTATTTATACTGATGGAGCATGTTCTGGTAACCCTGGTCCTGGAGGATGGGCAGC
AGTTTTGCTGTTTGATGATAATGAAAAGACAATCTGTGGTAATGATTCCGATACTACAAATAATAGGATGGAGTTAACAG
CGGTTATTGAGGCACTGAAGTTATTAAAAGTAGCATATAATGTTGATTTGTATACTGATAGCGTATATGTTAAAGATGGT
ATTACATTGTGGATCAGGAAATGGAAGGTTAATGGATGGAAGACTGCTAATAAGATGCCAGTGAAAAACTTGGAACTGTG
GTTAGAATTAGATAGTCTCGCTAATTTTCATAAAGTTACTTGGTATTGGGTTAGAGCTCATGTAGGGGATTTGTATAATC
AGAAAGCAGATATGCTTGCACGTAGTCAGATTGTCAGGTGA

Upstream 100 bases:

>100_bases
GTATATTTGTTATTGTATGAATAGATGCTTGTTTTCAGTGTTGATATAGTCAATATTAGATGTTATCCTTAAGATCAGGA
TTGTTTTGTATATATTTAAC

Downstream 100 bases:

>100_bases
ATTTTATCATGTCCTTATGTTTACATTTCTAGAACATTAGATCAGTGTAGTAAAGTAAATATATCTTTTGAGTAATATGT
ATAGATAACACCGCTGTGTA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MKDELNKVVIYTDGACSGNPGPGGWAAVLLFDDNEKTICGNDSDTTNNRMELTAVIEALKLLKVAYNVDLYTDSVYVKDG
ITLWIRKWKVNGWKTANKMPVKNLELWLELDSLANFHKVTWYWVRAHVGDLYNQKADMLARSQIVR

Sequences:

>Translated_146_residues
MKDELNKVVIYTDGACSGNPGPGGWAAVLLFDDNEKTICGNDSDTTNNRMELTAVIEALKLLKVAYNVDLYTDSVYVKDG
ITLWIRKWKVNGWKTANKMPVKNLELWLELDSLANFHKVTWYWVRAHVGDLYNQKADMLARSQIVR
>Mature_146_residues
MKDELNKVVIYTDGACSGNPGPGGWAAVLLFDDNEKTICGNDSDTTNNRMELTAVIEALKLLKVAYNVDLYTDSVYVKDG
ITLWIRKWKVNGWKTANKMPVKNLELWLELDSLANFHKVTWYWVRAHVGDLYNQKADMLARSQIVR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=149, Percent_Identity=35.5704697986577, Blast_Score=77, Evalue=7e-15,
Organism=Escherichia coli, GI1786408, Length=138, Percent_Identity=52.1739130434783, Blast_Score=152, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI115532542, Length=142, Percent_Identity=39.4366197183099, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17505482, Length=134, Percent_Identity=34.3283582089552, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI17137476, Length=142, Percent_Identity=37.3239436619718, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI45552509, Length=142, Percent_Identity=37.3239436619718, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_EHRCR (Q2GHJ9)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507084.1
- ProteinModelPortal:   Q2GHJ9
- STRING:   Q2GHJ9
- GeneID:   3927016
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0263
- TIGR:   ECH_0263
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q2GHJ9
- ProtClustDB:   PRK00203
- BioCyc:   ECHA205920:ECH_0263-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16638; Mature: 16638

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDELNKVVIYTDGACSGNPGPGGWAAVLLFDDNEKTICGNDSDTTNNRMELTAVIEALK
CCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCCEEHHHHHHHHHH
LLKVAYNVDLYTDSVYVKDGITLWIRKWKVNGWKTANKMPVKNLELWLELDSLANFHKVT
HHHHHHCEEEEECEEEEECCEEEEEEEEECCCEECCCCCCCCCEEEEEEECCCCCCHHEE
WYWVRAHVGDLYNQKADMLARSQIVR
EEEEEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDELNKVVIYTDGACSGNPGPGGWAAVLLFDDNEKTICGNDSDTTNNRMELTAVIEALK
CCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCCCCEEHHHHHHHHHH
LLKVAYNVDLYTDSVYVKDGITLWIRKWKVNGWKTANKMPVKNLELWLELDSLANFHKVT
HHHHHHCEEEEECEEEEECCEEEEEEEEECCCEECCCCCCCCCEEEEEEECCCCCCHHEE
WYWVRAHVGDLYNQKADMLARSQIVR
EEEEEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA