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The map label for this gene is pepE [H]

Identifier: 88657638

GI number: 88657638

Start: 458144

End: 459868

Strand: Direct

Name: pepE [H]

Synonym: ECH_0479

Alternate gene names: 88657638

Gene position: 458144-459868 (Clockwise)

Preceding gene: 88658231

Following gene: 88657586

Centisome position: 38.95

GC content: 30.49

Gene sequence:

>1725_bases
ATGAAAAATAGGTTAAATCAATTAATTGGTTTAATGGAAGAGTATGAAATTGATGTATTGTTATTGCAGAATACGGATGA
ATATCAGTGTGAGTATGTGCATATTAACAAGCAAAGAATAAGATGGTTGTGTGGTTTTTCTGGCTCAAATGCTACTTTGA
TAATATCAAGGGAAGGTAAACAGAATTTTTTTACTGATGGTAGATATACATTACAAGCAACAAGGGAGTTGGACTGTAGT
TATTATCAAATACATAATGTGTGTGAGTTAACTCCTTGGCAATGGTGTGTAGAAAATTGTCTATCTCATACTGTTGTAGC
TTATGAATCTGCATTGTTTACTTTGAGTCAAATAAGAAAGTATGAAGATTGTGGTATTTTTTTAAAACCAATAGATCAAA
TTTTGATTGATAAGTTGTGGATTCGTGATTTTGCTATAGAACATGATATAGTACAGCATTCTTTAGAATATTCTGGTGTT
GAGAGTTATACAAAGTCTTGTGAAGTTGCAAAATATTTGTCTGACAAAGATGCTGCATTAATCACAAATACTGATGTTAT
TTCATGGATGTTAAATATACGTAATAAAAAGTTTCTTTATAATCCTTCAGTGTTGTCTAGAGCGATTTTATATAAGGATG
GAAGAGTTGATTTATTTATTGATGATGTTTACTCAGTAAATGTTAAATATGAGCATCTTAATATATGTTCTTTGAATAAT
CTATTTAATGTTTTGAAATCTGTAAAATCAGTAGTTGTAGATGCATCTACTATACCAATGAGTATTTTTCTATCTTTACA
ACAACAGGATGTATTAGTTAATGATGCAGATTTTTGTCTTTTAATGAAAGCCAGAAAGAATGATGTTGAAATACAAGGTG
CAATTAATGCTCATGTTAGAGATGGAATTTCTATAGTTAATTTATTATACTGGTTAAATATGCAATTGGATAATAATCAG
AAAATTACAGAGCTGGATGTTGAGTCAAAATTATTAGATTTCAGAAAACAGCAAAGTTTGTTTCAAGGTGAAAGTTTTTC
TACAATTTCTGGTTTTCAAGAAAACGGAGCTGTAATACATTACAGAGCGAATAATGATACAAATAAGTTAATATGTAAGA
ATGGATTATATTTATTAGATTCTGGTGGTCAATATCTTGATGGTACAACAGATGTTACGCGTACTGTTGCTATAGGTGAA
CCTACATCTGAGCAAATTACTAATTTTACGTTAGTTTTAAAAGGTCATATTGCTTTAGCGATGGCAGTCTTTCCTTTAGG
TACTACTGGTGGAATGTTAGATATATTAGCTAGACAGTATTTGTGGAAATCAGGACTTGATTATCAACATGGTACGGGTC
ATGGGGTGGGAAGTTTTTTATCAGTTCATGAAGGTCCTTGTGCTATTTCGTACAAGAATGATGTTGTATTGCAGCCAAAT
ATGGTGCTATCAAATGAGCCAGGATATTATAAAAATGGTGAATATGGAATAAGGATTGAAAATCTGATGTACGTTGAAGA
ATATATGAATGGCTTTTTAAGATTTAAACAGTTAACATGTGTGCCTATAGATTTAAGATTAATAGATGTAGATATGTTAA
ATCATGAGGAAATCAATTACATAGACCAGTATCATAATTTTGTATATAACACTATTGCTCCACATGTAAGTGAAGAAGTA
AAACATTGGTTATGTCATGCATGTCAGAGTTTAAAAGGTAAGTAG

Upstream 100 bases:

>100_bases
ATAAGATTGTTAGTAGTAAGTTTGTAGTTGATAAAAAAGGCTGCTGTGCCTTGTATTATGTAGTAATATGTATCTTTATT
ATTTATCAAGTGTGAAATTT

Downstream 100 bases:

>100_bases
ATTTGTTTTATAAGCACTTTTTGTATGTTTGAATATTTTTGTTTTTTAGGTATATCTAAATCATAACCTTATGTATGAAA
TAATAGCTGTATTCTATTGT

Product: M24 family metallopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MKNRLNQLIGLMEEYEIDVLLLQNTDEYQCEYVHINKQRIRWLCGFSGSNATLIISREGKQNFFTDGRYTLQATRELDCS
YYQIHNVCELTPWQWCVENCLSHTVVAYESALFTLSQIRKYEDCGIFLKPIDQILIDKLWIRDFAIEHDIVQHSLEYSGV
ESYTKSCEVAKYLSDKDAALITNTDVISWMLNIRNKKFLYNPSVLSRAILYKDGRVDLFIDDVYSVNVKYEHLNICSLNN
LFNVLKSVKSVVVDASTIPMSIFLSLQQQDVLVNDADFCLLMKARKNDVEIQGAINAHVRDGISIVNLLYWLNMQLDNNQ
KITELDVESKLLDFRKQQSLFQGESFSTISGFQENGAVIHYRANNDTNKLICKNGLYLLDSGGQYLDGTTDVTRTVAIGE
PTSEQITNFTLVLKGHIALAMAVFPLGTTGGMLDILARQYLWKSGLDYQHGTGHGVGSFLSVHEGPCAISYKNDVVLQPN
MVLSNEPGYYKNGEYGIRIENLMYVEEYMNGFLRFKQLTCVPIDLRLIDVDMLNHEEINYIDQYHNFVYNTIAPHVSEEV
KHWLCHACQSLKGK

Sequences:

>Translated_574_residues
MKNRLNQLIGLMEEYEIDVLLLQNTDEYQCEYVHINKQRIRWLCGFSGSNATLIISREGKQNFFTDGRYTLQATRELDCS
YYQIHNVCELTPWQWCVENCLSHTVVAYESALFTLSQIRKYEDCGIFLKPIDQILIDKLWIRDFAIEHDIVQHSLEYSGV
ESYTKSCEVAKYLSDKDAALITNTDVISWMLNIRNKKFLYNPSVLSRAILYKDGRVDLFIDDVYSVNVKYEHLNICSLNN
LFNVLKSVKSVVVDASTIPMSIFLSLQQQDVLVNDADFCLLMKARKNDVEIQGAINAHVRDGISIVNLLYWLNMQLDNNQ
KITELDVESKLLDFRKQQSLFQGESFSTISGFQENGAVIHYRANNDTNKLICKNGLYLLDSGGQYLDGTTDVTRTVAIGE
PTSEQITNFTLVLKGHIALAMAVFPLGTTGGMLDILARQYLWKSGLDYQHGTGHGVGSFLSVHEGPCAISYKNDVVLQPN
MVLSNEPGYYKNGEYGIRIENLMYVEEYMNGFLRFKQLTCVPIDLRLIDVDMLNHEEINYIDQYHNFVYNTIAPHVSEEV
KHWLCHACQSLKGK
>Mature_574_residues
MKNRLNQLIGLMEEYEIDVLLLQNTDEYQCEYVHINKQRIRWLCGFSGSNATLIISREGKQNFFTDGRYTLQATRELDCS
YYQIHNVCELTPWQWCVENCLSHTVVAYESALFTLSQIRKYEDCGIFLKPIDQILIDKLWIRDFAIEHDIVQHSLEYSGV
ESYTKSCEVAKYLSDKDAALITNTDVISWMLNIRNKKFLYNPSVLSRAILYKDGRVDLFIDDVYSVNVKYEHLNICSLNN
LFNVLKSVKSVVVDASTIPMSIFLSLQQQDVLVNDADFCLLMKARKNDVEIQGAINAHVRDGISIVNLLYWLNMQLDNNQ
KITELDVESKLLDFRKQQSLFQGESFSTISGFQENGAVIHYRANNDTNKLICKNGLYLLDSGGQYLDGTTDVTRTVAIGE
PTSEQITNFTLVLKGHIALAMAVFPLGTTGGMLDILARQYLWKSGLDYQHGTGHGVGSFLSVHEGPCAISYKNDVVLQPN
MVLSNEPGYYKNGEYGIRIENLMYVEEYMNGFLRFKQLTCVPIDLRLIDVDMLNHEEINYIDQYHNFVYNTIAPHVSEEV
KHWLCHACQSLKGK

Specific function: Unknown

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24B family [H]

Homologues:

Organism=Homo sapiens, GI264681563, Length=616, Percent_Identity=34.5779220779221, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI264681565, Length=616, Percent_Identity=33.1168831168831, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI93141226, Length=607, Percent_Identity=29.159802306425, Blast_Score=278, Evalue=1e-74,
Organism=Escherichia coli, GI1788728, Length=191, Percent_Identity=31.413612565445, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17509539, Length=604, Percent_Identity=33.7748344370861, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25149105, Length=610, Percent_Identity=28.6885245901639, Blast_Score=205, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6322999, Length=625, Percent_Identity=32.32, Blast_Score=307, Evalue=2e-84,
Organism=Drosophila melanogaster, GI17137632, Length=590, Percent_Identity=34.0677966101695, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI161078230, Length=592, Percent_Identity=28.7162162162162, Blast_Score=217, Evalue=2e-56,
Organism=Drosophila melanogaster, GI21357287, Length=592, Percent_Identity=28.7162162162162, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR000994
- InterPro:   IPR001131 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 65707; Mature: 65707

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRLNQLIGLMEEYEIDVLLLQNTDEYQCEYVHINKQRIRWLCGFSGSNATLIISREGK
CCCHHHHHHHHHHHCCEEEEEEECCCCCEEEEEEECHHHEEEEEECCCCCEEEEEEECCC
QNFFTDGRYTLQATRELDCSYYQIHNVCELTPWQWCVENCLSHTVVAYESALFTLSQIRK
CCCCCCCEEEEEEECCCCCCHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEDCGIFLKPIDQILIDKLWIRDFAIEHDIVQHSLEYSGVESYTKSCEVAKYLSDKDAAL
HHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEE
ITNTDVISWMLNIRNKKFLYNPSVLSRAILYKDGRVDLFIDDVYSVNVKYEHLNICSLNN
EECHHHHHHHHHCCCCEEEECHHHHHHHHEEECCCEEEEEECEEEEEEEEEEEEEEEHHH
LFNVLKSVKSVVVDASTIPMSIFLSLQQQDVLVNDADFCLLMKARKNDVEIQGAINAHVR
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCCCEEEEEECCCCCEEEEEEEEHHHH
DGISIVNLLYWLNMQLDNNQKITELDVESKLLDFRKQQSLFQGESFSTISGFQENGAVIH
CCHHHHHHHHHEEEEECCCCCEEEECHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEEE
YRANNDTNKLICKNGLYLLDSGGQYLDGTTDVTRTVAIGEPTSEQITNFTLVLKGHIALA
EECCCCCCEEEEECCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHCEEEEEEECHHEEE
MAVFPLGTTGGMLDILARQYLWKSGLDYQHGTGHGVGSFLSVHEGPCAISYKNDVVLQPN
EHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEECCCCEEEEECCCEEECCC
MVLSNEPGYYKNGEYGIRIENLMYVEEYMNGFLRFKQLTCVPIDLRLIDVDMLNHEEINY
EEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEEECCCCHHHH
IDQYHNFVYNTIAPHVSEEVKHWLCHACQSLKGK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNRLNQLIGLMEEYEIDVLLLQNTDEYQCEYVHINKQRIRWLCGFSGSNATLIISREGK
CCCHHHHHHHHHHHCCEEEEEEECCCCCEEEEEEECHHHEEEEEECCCCCEEEEEEECCC
QNFFTDGRYTLQATRELDCSYYQIHNVCELTPWQWCVENCLSHTVVAYESALFTLSQIRK
CCCCCCCEEEEEEECCCCCCHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEDCGIFLKPIDQILIDKLWIRDFAIEHDIVQHSLEYSGVESYTKSCEVAKYLSDKDAAL
HHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEE
ITNTDVISWMLNIRNKKFLYNPSVLSRAILYKDGRVDLFIDDVYSVNVKYEHLNICSLNN
EECHHHHHHHHHCCCCEEEECHHHHHHHHEEECCCEEEEEECEEEEEEEEEEEEEEEHHH
LFNVLKSVKSVVVDASTIPMSIFLSLQQQDVLVNDADFCLLMKARKNDVEIQGAINAHVR
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCCCEEEEEECCCCCEEEEEEEEHHHH
DGISIVNLLYWLNMQLDNNQKITELDVESKLLDFRKQQSLFQGESFSTISGFQENGAVIH
CCHHHHHHHHHEEEEECCCCCEEEECHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEEE
YRANNDTNKLICKNGLYLLDSGGQYLDGTTDVTRTVAIGEPTSEQITNFTLVLKGHIALA
EECCCCCCEEEEECCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHCEEEEEEECHHEEE
MAVFPLGTTGGMLDILARQYLWKSGLDYQHGTGHGVGSFLSVHEGPCAISYKNDVVLQPN
EHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEECCCCEEEEECCCEEECCC
MVLSNEPGYYKNGEYGIRIENLMYVEEYMNGFLRFKQLTCVPIDLRLIDVDMLNHEEINY
EEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEEECCCCHHHH
IDQYHNFVYNTIAPHVSEEVKHWLCHACQSLKGK
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]