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The map label for this gene is mdh

Identifier: 88657609

GI number: 88657609

Start: 645953

End: 646894

Strand: Reverse

Name: mdh

Synonym: ECH_0641

Alternate gene names: 88657609

Gene position: 646894-645953 (Counterclockwise)

Preceding gene: 88657839

Following gene: 88658578

Centisome position: 55.0

GC content: 33.44

Gene sequence:

>942_bases
ATGATAAAACGTAAAAAGATTGCTCTTATTGGTGCAGGTAGTATAGGTGGCATGATAGCATATTTAGTTAGGTCACGAAA
TTTAGGAGATGTCGTTTTATTAGATGTCAATGGTGGTATAGCCAAAGGTAAAGCATTAGATATTGCTGAATCTTCACCAG
TAGCAAAACATAATGGAGAGATATTAGGTACTAACAATTATGCTGATATCGAAGGTGCTGATGCAATCATTGTTACTGCT
GGAATATCTAGAAAGCCCGGAATGAGCCGTGATGATCTGATTAATACTAATGTGCATGTTATAAAAGAAGTAGCAGAAAA
TATTGCTAAATATGCTCCTAATGCATTTGTTGTAGTAGTCACTAATCCACTTGATATAATGGTTTTAGCTATGCATAAAT
ATTCTCATTTGCCAAGTAATATGGTTGTTGGTATGGCTGGGGTACTTGATGCAGCAAGGTTCTCTTATTTTATTGCAAAA
GAATTGAATGTATCAGTAGATAGTGTGAGTTCTATAGTATTGGGTGGCCACGGAGATTTTATGCTTCCTTTAGTTAAGTA
CTCATCAGTTGGTGGGATATCTATTGCTGATCTGGTGAAAATGAATTTAATTACACAAGATAGGGTTAATGAGATTATAG
AAAAAACTAGAAAAGGTGGAGAAGAAATAGTAAATTTATTAAAGGTAGGTTCAGCTTATTATGCACCTGCTGAGTCTGCT
TTATTGATGGTTGACTCTTATTTGAATGATAGAAGATTAATGTTATCTTGCTCTGTTTATTTAAAGGGAGAGTATGGAGT
TCATGATCTATTTGTAGGTGTTCCTGTAATTATTGGTAAAAATGGAGTGGAAAAAGTAATAGAACTTCAACTAACTGAGG
AGGAAAAGAATGTATTTAATGACTCTGTTATGTCAATTAGAAAGTTAGTAAGTAATATTTAA

Upstream 100 bases:

>100_bases
AAACTACGAAAAGTAATAGTTAGTTAGATTAAGTATTGTATATATTCTATAATCAGGTAAACTTCATAAGTAGTGGTCTT
GAAATAAAGTAGAGGAAATA

Downstream 100 bases:

>100_bases
TGCTTGTTTATAAGCTTTTATTATAATCTGAATTTCATGTTTTAATTATTATATAAGCATATCGTAGTGTGATCTTGAAT
GATAATGCATATTTTTTATA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MIKRKKIALIGAGSIGGMIAYLVRSRNLGDVVLLDVNGGIAKGKALDIAESSPVAKHNGEILGTNNYADIEGADAIIVTA
GISRKPGMSRDDLINTNVHVIKEVAENIAKYAPNAFVVVVTNPLDIMVLAMHKYSHLPSNMVVGMAGVLDAARFSYFIAK
ELNVSVDSVSSIVLGGHGDFMLPLVKYSSVGGISIADLVKMNLITQDRVNEIIEKTRKGGEEIVNLLKVGSAYYAPAESA
LLMVDSYLNDRRLMLSCSVYLKGEYGVHDLFVGVPVIIGKNGVEKVIELQLTEEEKNVFNDSVMSIRKLVSNI

Sequences:

>Translated_313_residues
MIKRKKIALIGAGSIGGMIAYLVRSRNLGDVVLLDVNGGIAKGKALDIAESSPVAKHNGEILGTNNYADIEGADAIIVTA
GISRKPGMSRDDLINTNVHVIKEVAENIAKYAPNAFVVVVTNPLDIMVLAMHKYSHLPSNMVVGMAGVLDAARFSYFIAK
ELNVSVDSVSSIVLGGHGDFMLPLVKYSSVGGISIADLVKMNLITQDRVNEIIEKTRKGGEEIVNLLKVGSAYYAPAESA
LLMVDSYLNDRRLMLSCSVYLKGEYGVHDLFVGVPVIIGKNGVEKVIELQLTEEEKNVFNDSVMSIRKLVSNI
>Mature_313_residues
MIKRKKIALIGAGSIGGMIAYLVRSRNLGDVVLLDVNGGIAKGKALDIAESSPVAKHNGEILGTNNYADIEGADAIIVTA
GISRKPGMSRDDLINTNVHVIKEVAENIAKYAPNAFVVVVTNPLDIMVLAMHKYSHLPSNMVVGMAGVLDAARFSYFIAK
ELNVSVDSVSSIVLGGHGDFMLPLVKYSSVGGISIADLVKMNLITQDRVNEIIEKTRKGGEEIVNLLKVGSAYYAPAESA
LLMVDSYLNDRRLMLSCSVYLKGEYGVHDLFVGVPVIIGKNGVEKVIELQLTEEEKNVFNDSVMSIRKLVSNI

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=314, Percent_Identity=32.484076433121, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI221136809, Length=314, Percent_Identity=32.484076433121, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI15082234, Length=314, Percent_Identity=28.0254777070064, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI5031857, Length=313, Percent_Identity=30.0319488817891, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI260099723, Length=313, Percent_Identity=30.0319488817891, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI9257228, Length=312, Percent_Identity=29.4871794871795, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4504973, Length=312, Percent_Identity=29.4871794871795, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI291575128, Length=298, Percent_Identity=28.8590604026846, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI4557032, Length=298, Percent_Identity=28.8590604026846, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI260099727, Length=196, Percent_Identity=32.6530612244898, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=32.6530612244898, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI21735621, Length=317, Percent_Identity=26.4984227129338, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI207028494, Length=197, Percent_Identity=29.9492385786802, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI103472011, Length=299, Percent_Identity=24.0802675585284, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1789632, Length=317, Percent_Identity=29.3375394321767, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17535107, Length=300, Percent_Identity=26, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17554310, Length=325, Percent_Identity=28, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322765, Length=301, Percent_Identity=28.9036544850498, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=276, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324446, Length=253, Percent_Identity=29.2490118577075, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17136226, Length=294, Percent_Identity=27.5510204081633, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45550422, Length=298, Percent_Identity=27.1812080536913, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24647881, Length=316, Percent_Identity=29.4303797468354, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24663595, Length=301, Percent_Identity=27.5747508305648, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24663599, Length=245, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_EHRCR (Q2GGI2)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507451.1
- ProteinModelPortal:   Q2GGI2
- SMR:   Q2GGI2
- STRING:   Q2GGI2
- GeneID:   3927483
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0641
- TIGR:   ECH_0641
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   LDKPAND
- PhylomeDB:   Q2GGI2
- ProtClustDB:   PRK06223
- BioCyc:   ECHA205920:ECH_0641-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33689; Mature: 33689

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: ACT_SITE 177-177 BINDING 35-35 BINDING 84-84 BINDING 90-90 BINDING 97-97 BINDING 122-122 BINDING 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRKKIALIGAGSIGGMIAYLVRSRNLGDVVLLDVNGGIAKGKALDIAESSPVAKHNGE
CCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCE
ILGTNNYADIEGADAIIVTAGISRKPGMSRDDLINTNVHVIKEVAENIAKYAPNAFVVVV
EEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEE
TNPLDIMVLAMHKYSHLPSNMVVGMAGVLDAARFSYFIAKELNVSVDSVSSIVLGGHGDF
ECCHHEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEECCCCCE
MLPLVKYSSVGGISIADLVKMNLITQDRVNEIIEKTRKGGEEIVNLLKVGSAYYAPAESA
EEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
LLMVDSYLNDRRLMLSCSVYLKGEYGVHDLFVGVPVIIGKNGVEKVIELQLTEEEKNVFN
EEEEEHHCCCCEEEEEEEEEEECCCCHHHHHHCCEEEECCCCHHHHEEEEECCHHHHHHH
DSVMSIRKLVSNI
HHHHHHHHHHHCC
>Mature Secondary Structure
MIKRKKIALIGAGSIGGMIAYLVRSRNLGDVVLLDVNGGIAKGKALDIAESSPVAKHNGE
CCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCE
ILGTNNYADIEGADAIIVTAGISRKPGMSRDDLINTNVHVIKEVAENIAKYAPNAFVVVV
EEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEE
TNPLDIMVLAMHKYSHLPSNMVVGMAGVLDAARFSYFIAKELNVSVDSVSSIVLGGHGDF
ECCHHEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEECCCCCE
MLPLVKYSSVGGISIADLVKMNLITQDRVNEIIEKTRKGGEEIVNLLKVGSAYYAPAESA
EEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
LLMVDSYLNDRRLMLSCSVYLKGEYGVHDLFVGVPVIIGKNGVEKVIELQLTEEEKNVFN
EEEEEHHCCCCEEEEEEEEEEECCCCHHHHHHCCEEEECCCCHHHHEEEEECCHHHHHHH
DSVMSIRKLVSNI
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA