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The map label for this gene is ispE

Identifier: 88657607

GI number: 88657607

Start: 763133

End: 763981

Strand: Reverse

Name: ispE

Synonym: ECH_0757

Alternate gene names: 88657607

Gene position: 763981-763133 (Counterclockwise)

Preceding gene: 88658374

Following gene: 88658525

Centisome position: 64.95

GC content: 32.39

Gene sequence:

>849_bases
ATGTTAAAGTTTTTAGTTAAAGCACCAGCAAAAGTTAATTTATTTTTGCATATTACTGGTAAACGGAGTGATCAACATCA
TTACTTAGAGTCATTGTTTGTCTTTGTTAATGTTTACGATATCTTAGAGGTTGATGTTGGTGGTTCGAAACGGGGAGTAT
ATTTCTCAAATCTGAGGATTAGCAAATATAATAATACTGTATATAAAGCAATAGAGTTGTTATTGAAACACAGTGCTGTA
TGTCCTAATGTTTCTGTGAGTATTATAAAAAATATTTTAGTATCTGCAGGTTTAGCAGGTGGATCAGCTGATGCTGCTGC
TATTATGCGTTTACTAGGTAATATGTGGAACATTGATTACACATTGTTACAGGATTTAGCCTTAAAAATTGGAAGTGATG
TTCCAGCATGTTTGGAATCAAAGACCCTTTTTGCTAAAGGGGTAGGGGAAGATATATTGTTATTACCTGATTTGTTATTG
CCAAAATATATTATACTTGTTGCTCCAAGGGGAAAGACGTTAAGTACAGCAAAGGTCTTTAATAATTACCAGAGTGCTAC
TTACTCTCCTTCCATATGTGATAAGCTTCCTGTAAAGCAAGATGATTGGATGGAATTAATTTGTAATGCTAAAAATGATT
TATTAGAAGTAGCGTTGAAATTTGTTCCTGAGATAGAAGAAATATTATTTGTATTAAAGCAATTAAAAAATTCTGTAATT
GCTCGTATGACTGGTAGTGGAGCAACTTGTTTTGCTTTATTTAATGAATTAAGTCATGCTGAAGATGCAGCTAGAAAATT
GCAGATGACACGTCCTGATTGGATAATTTTTAATGCTAAAATACTTTGA

Upstream 100 bases:

>100_bases
TTAAAAGGATTTTTTATTTAATAATCACTAAAGATATTTTACAAGCTATGTAGTCTTCACTATTAATAGTGTATAGTATA
TTGTTAATGATAATATAATT

Downstream 100 bases:

>100_bases
ATTTTCTTTTGACAATTATTAGTGGATTTATTTCCTGTATAAGCTTATTGTCTGATGAAATTATAATAATGACTATAGTG
TATAGGAAAGTGAACTCTTT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MLKFLVKAPAKVNLFLHITGKRSDQHHYLESLFVFVNVYDILEVDVGGSKRGVYFSNLRISKYNNTVYKAIELLLKHSAV
CPNVSVSIIKNILVSAGLAGGSADAAAIMRLLGNMWNIDYTLLQDLALKIGSDVPACLESKTLFAKGVGEDILLLPDLLL
PKYIILVAPRGKTLSTAKVFNNYQSATYSPSICDKLPVKQDDWMELICNAKNDLLEVALKFVPEIEEILFVLKQLKNSVI
ARMTGSGATCFALFNELSHAEDAARKLQMTRPDWIIFNAKIL

Sequences:

>Translated_282_residues
MLKFLVKAPAKVNLFLHITGKRSDQHHYLESLFVFVNVYDILEVDVGGSKRGVYFSNLRISKYNNTVYKAIELLLKHSAV
CPNVSVSIIKNILVSAGLAGGSADAAAIMRLLGNMWNIDYTLLQDLALKIGSDVPACLESKTLFAKGVGEDILLLPDLLL
PKYIILVAPRGKTLSTAKVFNNYQSATYSPSICDKLPVKQDDWMELICNAKNDLLEVALKFVPEIEEILFVLKQLKNSVI
ARMTGSGATCFALFNELSHAEDAARKLQMTRPDWIIFNAKIL
>Mature_282_residues
MLKFLVKAPAKVNLFLHITGKRSDQHHYLESLFVFVNVYDILEVDVGGSKRGVYFSNLRISKYNNTVYKAIELLLKHSAV
CPNVSVSIIKNILVSAGLAGGSADAAAIMRLLGNMWNIDYTLLQDLALKIGSDVPACLESKTLFAKGVGEDILLLPDLLL
PKYIILVAPRGKTLSTAKVFNNYQSATYSPSICDKLPVKQDDWMELICNAKNDLLEVALKFVPEIEEILFVLKQLKNSVI
ARMTGSGATCFALFNELSHAEDAARKLQMTRPDWIIFNAKIL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=277, Percent_Identity=29.2418772563177, Blast_Score=103, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_EHRCR (Q2GG78)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507555.1
- ProteinModelPortal:   Q2GG78
- SMR:   Q2GG78
- STRING:   Q2GG78
- GeneID:   3927481
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0757
- TIGR:   ECH_0757
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   ELAMIMQ
- PhylomeDB:   Q2GG78
- ProtClustDB:   PRK14610
- BioCyc:   ECHA205920:ECH_0757-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31277; Mature: 31277

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFLVKAPAKVNLFLHITGKRSDQHHYLESLFVFVNVYDILEVDVGGSKRGVYFSNLRI
CCCEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEE
SKYNNTVYKAIELLLKHSAVCPNVSVSIIKNILVSAGLAGGSADAAAIMRLLGNMWNIDY
ECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHH
TLLQDLALKIGSDVPACLESKTLFAKGVGEDILLLPDLLLPKYIILVAPRGKTLSTAKVF
HHHHHHHHHHCCCCCHHHCCCHHHHCCCCCCCEEHHHHHCCCEEEEECCCCCCCHHHHHH
NNYQSATYSPSICDKLPVKQDDWMELICNAKNDLLEVALKFVPEIEEILFVLKQLKNSVI
HCCCCCCCCCHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ARMTGSGATCFALFNELSHAEDAARKLQMTRPDWIIFNAKIL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
>Mature Secondary Structure
MLKFLVKAPAKVNLFLHITGKRSDQHHYLESLFVFVNVYDILEVDVGGSKRGVYFSNLRI
CCCEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEE
SKYNNTVYKAIELLLKHSAVCPNVSVSIIKNILVSAGLAGGSADAAAIMRLLGNMWNIDY
ECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHH
TLLQDLALKIGSDVPACLESKTLFAKGVGEDILLLPDLLLPKYIILVAPRGKTLSTAKVF
HHHHHHHHHHCCCCCHHHCCCHHHHCCCCCCCEEHHHHHCCCEEEEECCCCCCCHHHHHH
NNYQSATYSPSICDKLPVKQDDWMELICNAKNDLLEVALKFVPEIEEILFVLKQLKNSVI
HCCCCCCCCCHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ARMTGSGATCFALFNELSHAEDAARKLQMTRPDWIIFNAKIL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA