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The map label for this gene is plsX

Identifier: 88657589

GI number: 88657589

Start: 424074

End: 425087

Strand: Direct

Name: plsX

Synonym: ECH_0447

Alternate gene names: 88657589

Gene position: 424074-425087 (Clockwise)

Preceding gene: 88657733

Following gene: 88658133

Centisome position: 36.05

GC content: 33.73

Gene sequence:

>1014_bases
ATGAGCATAATATCAATTGCAGTGGATGCTATGGGTGGAGATTTTGCTCCTGAAGCAGTAGTAAGCGGGTTGGATTTTGC
TTTAACAAATTTATTAGATGACCAAAATGTTTCTTTTAATATATACGGGCAGGGAAGCCAAGTGTTGCCTATATTAGATA
AATATAAGGATTTAAAAGAACATAGTGTATTTATTGATACTCCAGAAGTAGTATTAGCAAATGATAAACCATCCTTTGCG
CTAAGAAAACGTCGATCTTCTAGTATGTGGTGTGCAATTGATAGTATAAAAAGTGGTGTAACCTCTGGAGTTGTATCTTC
TGGTAACACAGGTGCTTTAATGGCTATTTCACGTTTTTTGTTGGGAACACTTCCTAACATAGATCGTCCAGCGATTTGTA
CTGCTCTTCCTTCTAGAGGTGAGGAGTATTTTGTATTATTAGATTTGGGTGCAAATATTGAAAGCAGTTCAAATGCTTTA
TTTCAATTTGCTATTATGGGGAGTGCTTTTGCAAAAGCTGTGTTGAATATTGCTAGCCCTAAAGTAGCATTACTAAATGT
TGGCCAAGAAGAAGTTAAAGGCACAGATGTTATCAGAGAAGCATTTTTATTATTGAAACAAGCTGAAGGTAGGATAAATT
TTTGTGGGTATATTGAACCTATTGATATACTAGGAGATAAAGTTGATGTTGTAGTTACTGATGGATTTTGTGGGAATATT
GTATTAAAGGTAGCTGAGTCTATTGCATATACTTTTAAGTCAGTTTTTGAAAAGTCCGTTACTAGTTCTATAATATCAAA
ATTTGCAGGATTATTGCTAAAATCCCAGATGAAAAAAGATTTTATGAGGTTTAATCCTAAAATGTATAATGGGGCTATGT
TATTAGGTCTAAATGGTGTTGTGGTGAAAAGTCATGGAAATGCTGATAAGGTAGCTTTTGCTCATGCTATTAAAGTGACT
GTAAATGCTGTGCGTAATGATATCAATGCTAAAATTATTCATGAATTAAGTTAA

Upstream 100 bases:

>100_bases
TCTCATCATGTATGCTTAGGTGGATATTATAATGAGAAACAGGTATTAGAAGTAGATAGTAGTAATGTATAGTTAATTTA
ATTTTTTGGGAAGTATGTAA

Downstream 100 bases:

>100_bases
TGTTATTCTATGAAAAGATCTACTATACTGGGAATAGGCTCATATTTGCCTAAAAAAATAGTAACAAATGATGAACTTGC
TTTAACTGTTGAAACTAGTG

Product: putative glycerol-3-phosphate acyltransferase PlsX

Products: NA

Alternate protein names: Acyl-ACP phosphotransacylase; Acyl-[acyl-carrier-protein]--phosphate acyltransferase; Phosphate-acyl-ACP acyltransferase

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSIISIAVDAMGGDFAPEAVVSGLDFALTNLLDDQNVSFNIYGQGSQVLPILDKYKDLKEHSVFIDTPEVVLANDKPSFA
LRKRRSSSMWCAIDSIKSGVTSGVVSSGNTGALMAISRFLLGTLPNIDRPAICTALPSRGEEYFVLLDLGANIESSSNAL
FQFAIMGSAFAKAVLNIASPKVALLNVGQEEVKGTDVIREAFLLLKQAEGRINFCGYIEPIDILGDKVDVVVTDGFCGNI
VLKVAESIAYTFKSVFEKSVTSSIISKFAGLLLKSQMKKDFMRFNPKMYNGAMLLGLNGVVVKSHGNADKVAFAHAIKVT
VNAVRNDINAKIIHELS

Sequences:

>Translated_337_residues
MSIISIAVDAMGGDFAPEAVVSGLDFALTNLLDDQNVSFNIYGQGSQVLPILDKYKDLKEHSVFIDTPEVVLANDKPSFA
LRKRRSSSMWCAIDSIKSGVTSGVVSSGNTGALMAISRFLLGTLPNIDRPAICTALPSRGEEYFVLLDLGANIESSSNAL
FQFAIMGSAFAKAVLNIASPKVALLNVGQEEVKGTDVIREAFLLLKQAEGRINFCGYIEPIDILGDKVDVVVTDGFCGNI
VLKVAESIAYTFKSVFEKSVTSSIISKFAGLLLKSQMKKDFMRFNPKMYNGAMLLGLNGVVVKSHGNADKVAFAHAIKVT
VNAVRNDINAKIIHELS
>Mature_336_residues
SIISIAVDAMGGDFAPEAVVSGLDFALTNLLDDQNVSFNIYGQGSQVLPILDKYKDLKEHSVFIDTPEVVLANDKPSFAL
RKRRSSSMWCAIDSIKSGVTSGVVSSGNTGALMAISRFLLGTLPNIDRPAICTALPSRGEEYFVLLDLGANIESSSNALF
QFAIMGSAFAKAVLNIASPKVALLNVGQEEVKGTDVIREAFLLLKQAEGRINFCGYIEPIDILGDKVDVVVTDGFCGNIV
LKVAESIAYTFKSVFEKSVTSSIISKFAGLLLKSQMKKDFMRFNPKMYNGAMLLGLNGVVVKSHGNADKVAFAHAIKVTV
NAVRNDINAKIIHELS

Specific function: Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA

COG id: COG0416

COG function: function code I; Fatty acid/phospholipid biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm. Note=Associated with the membrane possibly through plsY (By similarity)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the plsX family

Homologues:

Organism=Escherichia coli, GI87081831, Length=341, Percent_Identity=41.0557184750733, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSX_EHRCR (Q2GH18)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507265.1
- ProteinModelPortal:   Q2GH18
- SMR:   Q2GH18
- STRING:   Q2GH18
- GeneID:   3927710
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0447
- TIGR:   ECH_0447
- eggNOG:   COG0416
- HOGENOM:   HBG288268
- OMA:   EGFSGNI
- PhylomeDB:   Q2GH18
- ProtClustDB:   PRK05331
- BioCyc:   ECHA205920:ECH_0447-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00019
- InterPro:   IPR003664
- InterPro:   IPR012281
- PIRSF:   PIRSF002465
- TIGRFAMs:   TIGR00182

Pfam domain/function: PF02504 FA_synthesis

EC number: NA

Molecular weight: Translated: 36251; Mature: 36120

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIISIAVDAMGGDFAPEAVVSGLDFALTNLLDDQNVSFNIYGQGSQVLPILDKYKDLKE
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHH
HSVFIDTPEVVLANDKPSFALRKRRSSSMWCAIDSIKSGVTSGVVSSGNTGALMAISRFL
CEEEEECCCEEEECCCCCHHHHHCCCCCCEEEHHHHHCCHHHCCCCCCCCCHHHHHHHHH
LGTLPNIDRPAICTALPSRGEEYFVLLDLGANIESSSNALFQFAIMGSAFAKAVLNIASP
HHCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHCCC
KVALLNVGQEEVKGTDVIREAFLLLKQAEGRINFCGYIEPIDILGDKVDVVVTDGFCGNI
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCEEEEEEECCCHHHH
VLKVAESIAYTFKSVFEKSVTSSIISKFAGLLLKSQMKKDFMRFNPKMYNGAMLLGLNGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEE
VVKSHGNADKVAFAHAIKVTVNAVRNDINAKIIHELS
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure 
SIISIAVDAMGGDFAPEAVVSGLDFALTNLLDDQNVSFNIYGQGSQVLPILDKYKDLKE
CEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHH
HSVFIDTPEVVLANDKPSFALRKRRSSSMWCAIDSIKSGVTSGVVSSGNTGALMAISRFL
CEEEEECCCEEEECCCCCHHHHHCCCCCCEEEHHHHHCCHHHCCCCCCCCCHHHHHHHHH
LGTLPNIDRPAICTALPSRGEEYFVLLDLGANIESSSNALFQFAIMGSAFAKAVLNIASP
HHCCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHCCC
KVALLNVGQEEVKGTDVIREAFLLLKQAEGRINFCGYIEPIDILGDKVDVVVTDGFCGNI
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCEEEEEEECCCHHHH
VLKVAESIAYTFKSVFEKSVTSSIISKFAGLLLKSQMKKDFMRFNPKMYNGAMLLGLNGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEE
VVKSHGNADKVAFAHAIKVTVNAVRNDINAKIIHELS
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA